Fringilla coelebs papillomavirus (isolate Chaffinch/Netherlands/Dutch) (FcPV)
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8JNA7|Q8JNA7_FCPVN Putative E7 protein OS=Fringilla coelebs papillomavirus (isolate Chaffinch/Netherlands/Dutch) OX=654914 GN=E7 PE=4 SV=1
MM1 pKa = 8.06 RR2 pKa = 11.84 NRR4 pKa = 11.84 LPIGAQGPPGGQPSNNPSEE23 pKa = 5.26 NNFNPEE29 pKa = 3.8 DD30 pKa = 3.88 WDD32 pKa = 3.67 ILLDD36 pKa = 3.8 TDD38 pKa = 4.1 SSSGSEE44 pKa = 4.21 TEE46 pKa = 4.09 TAEE49 pKa = 3.98 ATSPSSSEE57 pKa = 3.72 EE58 pKa = 3.77 SAEE61 pKa = 3.96 FDD63 pKa = 3.55 WEE65 pKa = 4.22 VKK67 pKa = 10.78 LIVTGNSQDD76 pKa = 4.34 PITLQEE82 pKa = 4.77 LEE84 pKa = 5.1 DD85 pKa = 4.12 LNQVLVAEE93 pKa = 5.25 LGHH96 pKa = 6.87 PATVYY101 pKa = 10.56 SIQDD105 pKa = 3.39 QADD108 pKa = 3.88 GASYY112 pKa = 10.38 PPGSPTPSTSTFPFEE127 pKa = 4.29 TPQGNNQFTSMAVAGSSAAADD148 pKa = 3.75 PPSFPSPASVVDD160 pKa = 4.68 LVCHH164 pKa = 6.35 EE165 pKa = 4.65 SMGDD169 pKa = 3.25 SDD171 pKa = 4.1 VDD173 pKa = 3.99 EE174 pKa = 5.24 EE175 pKa = 4.34 EE176 pKa = 4.76 HH177 pKa = 7.2 LPNNPANTPEE187 pKa = 3.86 EE188 pKa = 4.27 SGANDD193 pKa = 3.59 TEE195 pKa = 4.89 FKK197 pKa = 10.35 CTICSKK203 pKa = 10.35 PLTEE207 pKa = 5.31 GEE209 pKa = 3.91 LDD211 pKa = 3.07 EE212 pKa = 4.79 WGLVQGDD219 pKa = 3.5 EE220 pKa = 4.58 GLCHH224 pKa = 6.49 FCGFGAGVVDD234 pKa = 5.76 FFPP237 pKa = 5.61
Molecular weight: 25.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.592
IPC_protein 3.579
Toseland 3.376
ProMoST 3.732
Dawson 3.554
Bjellqvist 3.706
Wikipedia 3.465
Rodwell 3.414
Grimsley 3.287
Solomon 3.541
Lehninger 3.503
Nozaki 3.668
DTASelect 3.846
Thurlkill 3.427
EMBOSS 3.478
Sillero 3.694
Patrickios 0.985
IPC_peptide 3.541
IPC2_peptide 3.681
IPC2.peptide.svr19 3.664
Protein with the highest isoelectric point:
>tr|Q8JNA6|Q8JNA6_FCPVN Putative replication protein E1 OS=Fringilla coelebs papillomavirus (isolate Chaffinch/Netherlands/Dutch) OX=654914 GN=E1 PE=4 SV=1
MM1 pKa = 7.48 EE2 pKa = 5.89 LSNPQGLPQTDD13 pKa = 3.46 RR14 pKa = 11.84 CIGSCCSSNSSSSGMRR30 pKa = 11.84 LTLFCGSWCKK40 pKa = 10.52 VVAIGNGGHH49 pKa = 7.5 LPRR52 pKa = 11.84 IPMTQLLISTISRR65 pKa = 11.84 AQCRR69 pKa = 11.84 KK70 pKa = 9.5 LCTAAPYY77 pKa = 10.71 LFLKK81 pKa = 10.43 QCVCIHH87 pKa = 6.77 RR88 pKa = 11.84 MHH90 pKa = 7.02 CLLPIFSQLKK100 pKa = 9.3 RR101 pKa = 11.84 ICAGLPPEE109 pKa = 4.46 GWLGTVADD117 pKa = 4.63 KK118 pKa = 10.5 LTMFICQMQNEE129 pKa = 4.64 CLKK132 pKa = 10.18 HH133 pKa = 5.74 YY134 pKa = 9.92 PYY136 pKa = 9.15 EE137 pKa = 3.98 TLEE140 pKa = 3.64 IVMPYY145 pKa = 8.58 TALRR149 pKa = 11.84 EE150 pKa = 4.39 LYY152 pKa = 10.65 SHH154 pKa = 6.94 LQLTASQRR162 pKa = 11.84 RR163 pKa = 11.84 TKK165 pKa = 10.73 VSLILRR171 pKa = 11.84 DD172 pKa = 3.32 IRR174 pKa = 11.84 HH175 pKa = 5.7 CQLVRR180 pKa = 11.84 EE181 pKa = 4.82 SKK183 pKa = 9.77 HH184 pKa = 3.94 QARR187 pKa = 11.84 GRR189 pKa = 11.84 KK190 pKa = 7.61 DD191 pKa = 2.89 HH192 pKa = 7.08 RR193 pKa = 11.84 NYY195 pKa = 10.52 FNAAA199 pKa = 3.23
Molecular weight: 22.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.782
IPC2_protein 8.507
IPC_protein 8.39
Toseland 8.287
ProMoST 8.916
Dawson 9.121
Bjellqvist 9.399
Wikipedia 9.107
Rodwell 9.18
Grimsley 8.156
Solomon 9.224
Lehninger 9.224
Nozaki 9.663
DTASelect 9.121
Thurlkill 9.194
EMBOSS 9.297
Sillero 9.487
Patrickios 4.202
IPC_peptide 9.224
IPC2_peptide 8.916
IPC2.peptide.svr19 8.489
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2540
199
694
423.3
47.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.535 ± 0.366
2.047 ± 0.548
5.906 ± 0.338
6.339 ± 0.527
3.386 ± 0.36
7.205 ± 0.767
2.283 ± 0.387
4.291 ± 0.411
3.858 ± 0.87
8.583 ± 0.67
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.693 ± 0.176
4.409 ± 0.736
7.087 ± 0.936
4.173 ± 0.298
6.378 ± 0.767
8.543 ± 0.763
5.827 ± 0.221
6.535 ± 0.736
1.535 ± 0.122
3.386 ± 0.419
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here