Pseudoalteromonas amylolytica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4015 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S1N043|A0A1S1N043_9GAMM PspC domain-containing protein OS=Pseudoalteromonas amylolytica OX=1859457 GN=BET10_04505 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 9.85KK3 pKa = 10.15YY4 pKa = 10.44CIAAALSMACLAPVAQADD22 pKa = 4.25TLLGLYY28 pKa = 10.13LGAEE32 pKa = 4.97GWQADD37 pKa = 3.56NSGNFAEE44 pKa = 4.9SGNAQSFNFDD54 pKa = 3.82DD55 pKa = 4.03EE56 pKa = 5.05TFTSYY61 pKa = 10.9YY62 pKa = 10.51AALEE66 pKa = 4.23HH67 pKa = 6.5PVPFVPNIKK76 pKa = 10.12LRR78 pKa = 11.84YY79 pKa = 8.15TEE81 pKa = 5.21LEE83 pKa = 4.31LNGSTTLSEE92 pKa = 4.08TFEE95 pKa = 4.32FGGKK99 pKa = 10.03DD100 pKa = 3.52FIVGTTANTFTDD112 pKa = 4.75LSHH115 pKa = 7.76VDD117 pKa = 3.13YY118 pKa = 10.23TLYY121 pKa = 11.34YY122 pKa = 10.35EE123 pKa = 5.34IFDD126 pKa = 3.99NDD128 pKa = 3.68LVSFDD133 pKa = 4.12FGVNLKK139 pKa = 10.28QFDD142 pKa = 3.7GTIQVAGTVEE152 pKa = 4.56GNTTTEE158 pKa = 3.89TRR160 pKa = 11.84DD161 pKa = 3.43FSGVVPLGYY170 pKa = 10.44LRR172 pKa = 11.84AEE174 pKa = 4.82LGLPMTGFSVFAEE187 pKa = 4.6GSMLAVDD194 pKa = 4.09DD195 pKa = 5.77SKK197 pKa = 11.43IQDD200 pKa = 3.83YY201 pKa = 10.47QVGVAWQVIGNLAVDD216 pKa = 3.64VAVRR220 pKa = 11.84AGYY223 pKa = 10.48RR224 pKa = 11.84SMVLEE229 pKa = 5.05LDD231 pKa = 3.98DD232 pKa = 5.2VDD234 pKa = 6.45DD235 pKa = 4.59ISTDD239 pKa = 3.07IDD241 pKa = 3.54ASGPFAGIEE250 pKa = 3.85VHH252 pKa = 6.94FF253 pKa = 4.86

Molecular weight: 27.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S1MPJ4|A0A1S1MPJ4_9GAMM HTH araC/xylS-type domain-containing protein OS=Pseudoalteromonas amylolytica OX=1859457 GN=BET10_14955 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.93VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4015

0

4015

1409126

37

4761

351.0

39.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.63 ± 0.034

1.102 ± 0.014

5.551 ± 0.035

6.029 ± 0.035

4.162 ± 0.022

6.388 ± 0.044

2.467 ± 0.019

6.045 ± 0.027

5.409 ± 0.031

10.555 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.343 ± 0.016

4.494 ± 0.022

3.712 ± 0.024

5.287 ± 0.037

4.204 ± 0.028

6.867 ± 0.035

5.377 ± 0.037

6.884 ± 0.027

1.19 ± 0.014

3.303 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski