Amino acid dipepetide frequency for Pseudoalteromonas amylolytica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.999AlaAla: 6.999 ± 0.09	1.05AlaCys: 1.05 ± 0.028	4.442AlaAsp: 4.442 ± 0.065	4.453AlaGlu: 4.453 ± 0.068	3.431AlaPhe: 3.431 ± 0.053	5.488AlaGly: 5.488 ± 0.073	2.002AlaHis: 2.002 ± 0.044	5.765AlaIle: 5.765 ± 0.077	5.417AlaLys: 5.417 ± 0.074	10.219AlaLeu: 10.219 ± 0.091	2.418AlaMet: 2.418 ± 0.042	3.911AlaAsn: 3.911 ± 0.058	2.928AlaPro: 2.928 ± 0.054	5.387AlaGln: 5.387 ± 0.071	3.505AlaArg: 3.505 ± 0.055	5.46AlaSer: 5.46 ± 0.065	4.461AlaThr: 4.461 ± 0.075	5.467AlaVal: 5.467 ± 0.067	0.921AlaTrp: 0.921 ± 0.028	2.58AlaTyr: 2.58 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.897CysAla: 0.897 ± 0.026	0.158CysCys: 0.158 ± 0.011	0.706CysAsp: 0.706 ± 0.024	0.716CysGlu: 0.716 ± 0.026	0.506CysPhe: 0.506 ± 0.02	0.824CysGly: 0.824 ± 0.027	0.348CysHis: 0.348 ± 0.016	0.665CysIle: 0.665 ± 0.022	0.523CysLys: 0.523 ± 0.021	1.03CysLeu: 1.03 ± 0.03	0.229CysMet: 0.229 ± 0.013	0.441CysAsn: 0.441 ± 0.017	0.41CysPro: 0.41 ± 0.02	0.569CysGln: 0.569 ± 0.025	0.454CysArg: 0.454 ± 0.019	0.725CysSer: 0.725 ± 0.023	0.562CysThr: 0.562 ± 0.02	0.73CysVal: 0.73 ± 0.025	0.133CysTrp: 0.133 ± 0.01	0.397CysTyr: 0.397 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	4.564AspAla: 4.564 ± 0.061	0.549AspCys: 0.549 ± 0.022	3.261AspAsp: 3.261 ± 0.059	4.078AspGlu: 4.078 ± 0.061	2.506AspPhe: 2.506 ± 0.049	3.79AspGly: 3.79 ± 0.076	1.056AspHis: 1.056 ± 0.03	4.122AspIle: 4.122 ± 0.058	3.419AspLys: 3.419 ± 0.055	5.118AspLeu: 5.118 ± 0.063	1.391AspMet: 1.391 ± 0.034	2.759AspAsn: 2.759 ± 0.051	1.885AspPro: 1.885 ± 0.039	1.741AspGln: 1.741 ± 0.034	1.832AspArg: 1.832 ± 0.038	3.493AspSer: 3.493 ± 0.065	2.895AspThr: 2.895 ± 0.063	4.061AspVal: 4.061 ± 0.054	0.819AspTrp: 0.819 ± 0.024	2.173AspTyr: 2.173 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	4.768GluAla: 4.768 ± 0.061	0.559GluCys: 0.559 ± 0.023	2.709GluAsp: 2.709 ± 0.052	3.082GluGlu: 3.082 ± 0.061	2.618GluPhe: 2.618 ± 0.047	3.184GluGly: 3.184 ± 0.052	1.991GluHis: 1.991 ± 0.044	3.457GluIle: 3.457 ± 0.05	3.423GluLys: 3.423 ± 0.061	7.355GluLeu: 7.355 ± 0.083	1.35GluMet: 1.35 ± 0.03	2.453GluAsn: 2.453 ± 0.045	2.001GluPro: 2.001 ± 0.049	5.408GluGln: 5.408 ± 0.074	3.188GluArg: 3.188 ± 0.053	3.433GluSer: 3.433 ± 0.053	2.58GluThr: 2.58 ± 0.04	4.29GluVal: 4.29 ± 0.064	0.534GluTrp: 0.534 ± 0.021	1.91GluTyr: 1.91 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	3.976PheAla: 3.976 ± 0.061	0.51PheCys: 0.51 ± 0.02	2.956PheAsp: 2.956 ± 0.05	2.741PheGlu: 2.741 ± 0.048	1.632PhePhe: 1.632 ± 0.036	2.917PheGly: 2.917 ± 0.05	0.788PheHis: 0.788 ± 0.025	2.774PheIle: 2.774 ± 0.045	2.221PheLys: 2.221 ± 0.04	3.215PheLeu: 3.215 ± 0.054	0.988PheMet: 0.988 ± 0.028	2.274PheAsn: 2.274 ± 0.036	1.272PhePro: 1.272 ± 0.031	1.128PheGln: 1.128 ± 0.029	1.39PheArg: 1.39 ± 0.027	3.573PheSer: 3.573 ± 0.058	2.385PheThr: 2.385 ± 0.048	2.901PheVal: 2.901 ± 0.052	0.497PheTrp: 0.497 ± 0.019	1.481PheTyr: 1.481 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	5.041GlyAla: 5.041 ± 0.073	0.837GlyCys: 0.837 ± 0.026	3.578GlyAsp: 3.578 ± 0.086	4.075GlyGlu: 4.075 ± 0.054	3.119GlyPhe: 3.119 ± 0.05	4.293GlyGly: 4.293 ± 0.087	1.58GlyHis: 1.58 ± 0.039	4.112GlyIle: 4.112 ± 0.063	3.707GlyLys: 3.707 ± 0.062	6.432GlyLeu: 6.432 ± 0.079	1.683GlyMet: 1.683 ± 0.038	2.632GlyAsn: 2.632 ± 0.069	1.597GlyPro: 1.597 ± 0.035	2.942GlyGln: 2.942 ± 0.048	2.681GlyArg: 2.681 ± 0.044	3.912GlySer: 3.912 ± 0.065	3.369GlyThr: 3.369 ± 0.076	4.957GlyVal: 4.957 ± 0.071	0.841GlyTrp: 0.841 ± 0.026	2.486GlyTyr: 2.486 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	1.773HisAla: 1.773 ± 0.039	0.37HisCys: 0.37 ± 0.019	1.223HisAsp: 1.223 ± 0.04	1.292HisGlu: 1.292 ± 0.034	1.194HisPhe: 1.194 ± 0.03	1.634HisGly: 1.634 ± 0.035	0.83HisHis: 0.83 ± 0.03	1.652HisIle: 1.652 ± 0.031	1.233HisLys: 1.233 ± 0.032	2.414HisLeu: 2.414 ± 0.041	0.52HisMet: 0.52 ± 0.016	1.121HisAsn: 1.121 ± 0.026	1.148HisPro: 1.148 ± 0.03	1.391HisGln: 1.391 ± 0.034	0.983HisArg: 0.983 ± 0.028	1.708HisSer: 1.708 ± 0.043	1.328HisThr: 1.328 ± 0.031	1.461HisVal: 1.461 ± 0.035	0.405HisTrp: 0.405 ± 0.017	0.989HisTyr: 0.989 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.114IleAla: 6.114 ± 0.065	0.698IleCys: 0.698 ± 0.02	4.169IleAsp: 4.169 ± 0.059	4.604IleGlu: 4.604 ± 0.061	2.138IlePhe: 2.138 ± 0.041	3.997IleGly: 3.997 ± 0.063	1.297IleHis: 1.297 ± 0.032	3.125IleIle: 3.125 ± 0.051	3.594IleLys: 3.594 ± 0.058	4.931IleLeu: 4.931 ± 0.076	1.204IleMet: 1.204 ± 0.034	3.271IleAsn: 3.271 ± 0.049	2.203IlePro: 2.203 ± 0.04	2.129IleGln: 2.129 ± 0.036	2.505IleArg: 2.505 ± 0.045	4.474IleSer: 4.474 ± 0.052	3.663IleThr: 3.663 ± 0.066	3.833IleVal: 3.833 ± 0.057	0.665IleTrp: 0.665 ± 0.025	1.837IleTyr: 1.837 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	5.009LysAla: 5.009 ± 0.07	0.368LysCys: 0.368 ± 0.019	2.749LysAsp: 2.749 ± 0.053	3.237LysGlu: 3.237 ± 0.059	1.656LysPhe: 1.656 ± 0.036	3.212LysGly: 3.212 ± 0.052	1.578LysHis: 1.578 ± 0.035	2.829LysIle: 2.829 ± 0.045	2.922LysLys: 2.922 ± 0.056	5.847LysLeu: 5.847 ± 0.067	1.228LysMet: 1.228 ± 0.031	2.207LysAsn: 2.207 ± 0.039	2.299LysPro: 2.299 ± 0.048	3.684LysGln: 3.684 ± 0.051	2.832LysArg: 2.832 ± 0.055	3.396LysSer: 3.396 ± 0.058	2.869LysThr: 2.869 ± 0.047	4.063LysVal: 4.063 ± 0.056	0.568LysTrp: 0.568 ± 0.023	1.536LysTyr: 1.536 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	10.01LeuAla: 10.01 ± 0.097	1.284LeuCys: 1.284 ± 0.03	6.009LeuAsp: 6.009 ± 0.076	6.285LeuGlu: 6.285 ± 0.075	4.251LeuPhe: 4.251 ± 0.069	6.535LeuGly: 6.535 ± 0.078	2.22LeuHis: 2.22 ± 0.043	5.845LeuIle: 5.845 ± 0.08	5.738LeuLys: 5.738 ± 0.076	11.009LeuLeu: 11.009 ± 0.131	2.438LeuMet: 2.438 ± 0.043	5.088LeuAsn: 5.088 ± 0.062	4.616LeuPro: 4.616 ± 0.059	4.377LeuGln: 4.377 ± 0.061	4.193LeuArg: 4.193 ± 0.062	8.651LeuSer: 8.651 ± 0.085	6.113LeuThr: 6.113 ± 0.066	6.852LeuVal: 6.852 ± 0.074	1.021LeuTrp: 1.021 ± 0.034	3.015LeuTyr: 3.015 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	2.299MetAla: 2.299 ± 0.044	0.217MetCys: 0.217 ± 0.014	1.081MetAsp: 1.081 ± 0.028	1.04MetGlu: 1.04 ± 0.028	0.874MetPhe: 0.874 ± 0.025	1.458MetGly: 1.458 ± 0.03	0.508MetHis: 0.508 ± 0.019	1.259MetIle: 1.259 ± 0.029	1.21MetLys: 1.21 ± 0.029	2.706MetLeu: 2.706 ± 0.046	0.646MetMet: 0.646 ± 0.024	0.991MetAsn: 0.991 ± 0.025	1.114MetPro: 1.114 ± 0.035	1.241MetGln: 1.241 ± 0.031	1.149MetArg: 1.149 ± 0.033	1.893MetSer: 1.893 ± 0.04	1.394MetThr: 1.394 ± 0.03	1.55MetVal: 1.55 ± 0.032	0.226MetTrp: 0.226 ± 0.014	0.577MetTyr: 0.577 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.816AsnAla: 3.816 ± 0.052	0.455AsnCys: 0.455 ± 0.018	2.572AsnAsp: 2.572 ± 0.062	2.743AsnGlu: 2.743 ± 0.048	1.632AsnPhe: 1.632 ± 0.029	3.036AsnGly: 3.036 ± 0.058	1.077AsnHis: 1.077 ± 0.03	2.889AsnIle: 2.889 ± 0.043	2.485AsnLys: 2.485 ± 0.049	4.161AsnLeu: 4.161 ± 0.05	1.067AsnMet: 1.067 ± 0.027	2.29AsnAsn: 2.29 ± 0.041	1.947AsnPro: 1.947 ± 0.037	2.244AsnGln: 2.244 ± 0.042	1.949AsnArg: 1.949 ± 0.038	2.868AsnSer: 2.868 ± 0.047	2.731AsnThr: 2.731 ± 0.049	2.82AsnVal: 2.82 ± 0.044	0.615AsnTrp: 0.615 ± 0.022	1.544AsnTyr: 1.544 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	2.792ProAla: 2.792 ± 0.053	0.351ProCys: 0.351 ± 0.016	2.318ProAsp: 2.318 ± 0.045	2.78ProGlu: 2.78 ± 0.052	1.671ProPhe: 1.671 ± 0.036	1.96ProGly: 1.96 ± 0.042	0.947ProHis: 0.947 ± 0.028	2.37ProIle: 2.37 ± 0.041	2.093ProLys: 2.093 ± 0.04	3.885ProLeu: 3.885 ± 0.063	0.891ProMet: 0.891 ± 0.025	1.798ProAsn: 1.798 ± 0.037	1.035ProPro: 1.035 ± 0.033	1.808ProGln: 1.808 ± 0.036	1.21ProArg: 1.21 ± 0.032	2.606ProSer: 2.606 ± 0.038	2.011ProThr: 2.011 ± 0.042	2.772ProVal: 2.772 ± 0.049	0.49ProTrp: 0.49 ± 0.021	1.334ProTyr: 1.334 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	5.063GlnAla: 5.063 ± 0.077	0.511GlnCys: 0.511 ± 0.019	2.301GlnAsp: 2.301 ± 0.039	2.455GlnGlu: 2.455 ± 0.046	2.083GlnPhe: 2.083 ± 0.039	3.481GlnGly: 3.481 ± 0.052	1.497GlnHis: 1.497 ± 0.039	2.775GlnIle: 2.775 ± 0.046	2.293GlnLys: 2.293 ± 0.042	6.503GlnLeu: 6.503 ± 0.082	1.075GlnMet: 1.075 ± 0.03	1.892GlnAsn: 1.892 ± 0.036	1.862GlnPro: 1.862 ± 0.047	4.196GlnGln: 4.196 ± 0.09	2.389GlnArg: 2.389 ± 0.043	3.557GlnSer: 3.557 ± 0.06	2.702GlnThr: 2.702 ± 0.051	3.687GlnVal: 3.687 ± 0.057	0.791GlnTrp: 0.791 ± 0.029	1.76GlnTyr: 1.76 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	3.457ArgAla: 3.457 ± 0.051	0.433ArgCys: 0.433 ± 0.018	2.247ArgAsp: 2.247 ± 0.041	2.538ArgGlu: 2.538 ± 0.044	2.233ArgPhe: 2.233 ± 0.045	2.357ArgGly: 2.357 ± 0.041	1.086ArgHis: 1.086 ± 0.028	2.74ArgIle: 2.74 ± 0.055	2.115ArgLys: 2.115 ± 0.045	4.733ArgLeu: 4.733 ± 0.064	1.013ArgMet: 1.013 ± 0.027	1.768ArgAsn: 1.768 ± 0.035	1.448ArgPro: 1.448 ± 0.03	1.994ArgGln: 1.994 ± 0.039	1.953ArgArg: 1.953 ± 0.038	2.458ArgSer: 2.458 ± 0.044	2.086ArgThr: 2.086 ± 0.04	3.086ArgVal: 3.086 ± 0.053	0.542ArgTrp: 0.542 ± 0.017	1.747ArgTyr: 1.747 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	5.947SerAla: 5.947 ± 0.065	0.734SerCys: 0.734 ± 0.021	4.026SerAsp: 4.026 ± 0.065	4.442SerGlu: 4.442 ± 0.062	2.963SerPhe: 2.963 ± 0.049	4.901SerGly: 4.901 ± 0.07	1.74SerHis: 1.74 ± 0.04	4.055SerIle: 4.055 ± 0.063	3.603SerLys: 3.603 ± 0.055	7.08SerLeu: 7.08 ± 0.087	1.518SerMet: 1.518 ± 0.035	3.016SerAsn: 3.016 ± 0.048	2.31SerPro: 2.31 ± 0.04	3.553SerGln: 3.553 ± 0.055	2.625SerArg: 2.625 ± 0.042	4.693SerSer: 4.693 ± 0.077	3.467SerThr: 3.467 ± 0.064	4.744SerVal: 4.744 ± 0.066	0.826SerTrp: 0.826 ± 0.027	2.429SerTyr: 2.429 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	4.267ThrAla: 4.267 ± 0.076	0.55ThrCys: 0.55 ± 0.02	2.805ThrAsp: 2.805 ± 0.059	2.96ThrGlu: 2.96 ± 0.05	2.157ThrPhe: 2.157 ± 0.041	3.768ThrGly: 3.768 ± 0.066	1.485ThrHis: 1.485 ± 0.036	3.206ThrIle: 3.206 ± 0.064	2.417ThrLys: 2.417 ± 0.047	6.694ThrLeu: 6.694 ± 0.078	1.069ThrMet: 1.069 ± 0.029	2.135ThrAsn: 2.135 ± 0.041	2.7ThrPro: 2.7 ± 0.044	3.059ThrGln: 3.059 ± 0.044	2.111ThrArg: 2.111 ± 0.039	3.595ThrSer: 3.595 ± 0.066	2.956ThrThr: 2.956 ± 0.059	3.67ThrVal: 3.67 ± 0.068	0.598ThrTrp: 0.598 ± 0.021	1.563ThrTyr: 1.563 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	6.293ValAla: 6.293 ± 0.077	0.804ValCys: 0.804 ± 0.024	4.044ValAsp: 4.044 ± 0.059	4.496ValGlu: 4.496 ± 0.056	2.825ValPhe: 2.825 ± 0.046	4.245ValGly: 4.245 ± 0.059	1.324ValHis: 1.324 ± 0.032	4.391ValIle: 4.391 ± 0.063	3.763ValLys: 3.763 ± 0.062	6.885ValLeu: 6.885 ± 0.083	1.768ValMet: 1.768 ± 0.036	3.171ValAsn: 3.171 ± 0.052	2.488ValPro: 2.488 ± 0.05	2.522ValGln: 2.522 ± 0.04	2.721ValArg: 2.721 ± 0.051	5.133ValSer: 5.133 ± 0.057	4.105ValThr: 4.105 ± 0.069	5.075ValVal: 5.075 ± 0.066	0.723ValTrp: 0.723 ± 0.022	2.066ValTyr: 2.066 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	0.74TrpAla: 0.74 ± 0.022	0.142TrpCys: 0.142 ± 0.01	0.54TrpAsp: 0.54 ± 0.023	0.473TrpGlu: 0.473 ± 0.019	0.598TrpPhe: 0.598 ± 0.022	0.671TrpGly: 0.671 ± 0.028	0.449TrpHis: 0.449 ± 0.017	0.53TrpIle: 0.53 ± 0.021	0.358TrpLys: 0.358 ± 0.016	1.746TrpLeu: 1.746 ± 0.044	0.283TrpMet: 0.283 ± 0.015	0.414TrpAsn: 0.414 ± 0.019	0.472TrpPro: 0.472 ± 0.018	1.26TrpGln: 1.26 ± 0.034	0.67TrpArg: 0.67 ± 0.023	0.756TrpSer: 0.756 ± 0.029	0.429TrpThr: 0.429 ± 0.02	0.749TrpVal: 0.749 ± 0.025	0.188TrpTrp: 0.188 ± 0.012	0.434TrpTyr: 0.434 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.477TyrAla: 2.477 ± 0.041	0.442TyrCys: 0.442 ± 0.022	1.776TyrAsp: 1.776 ± 0.037	1.8TyrGlu: 1.8 ± 0.038	1.531TyrPhe: 1.531 ± 0.033	2.125TyrGly: 2.125 ± 0.041	0.865TyrHis: 0.865 ± 0.031	1.892TyrIle: 1.892 ± 0.037	1.554TyrLys: 1.554 ± 0.038	3.588TyrLeu: 3.588 ± 0.058	0.652TyrMet: 0.652 ± 0.025	1.309TyrAsn: 1.309 ± 0.032	1.387TyrPro: 1.387 ± 0.032	2.238TyrGln: 2.238 ± 0.04	1.696TyrArg: 1.696 ± 0.037	2.289TyrSer: 2.289 ± 0.043	1.661TyrThr: 1.661 ± 0.038	2.074TyrVal: 2.074 ± 0.04	0.501TyrTrp: 0.501 ± 0.018	1.175TyrTyr: 1.175 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4015 proteins (1409126 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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