Gluconobacter sp. DsW_058

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Gluconobacter; unclassified Gluconobacter

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2448 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A252BPF8|A0A252BPF8_9PROT Pyrroloquinoline-quinone synthase OS=Gluconobacter sp. DsW_058 OX=1511210 GN=pqqC PE=3 SV=1
MM1 pKa = 7.82SIINSLNTAVSGLNSQSHH19 pKa = 6.18AFSDD23 pKa = 4.26LSNNIANSQTTGYY36 pKa = 10.07KK37 pKa = 10.3AATTSFADD45 pKa = 3.64YY46 pKa = 9.25VTSNDD51 pKa = 3.53LASDD55 pKa = 4.37GEE57 pKa = 4.43SLSDD61 pKa = 3.54SVAATTRR68 pKa = 11.84QHH70 pKa = 6.29TDD72 pKa = 2.65NQGMVVSSTNTLALAISGNGFFNVVQPTGSTTSSTAPTFSSQQFYY117 pKa = 9.49TRR119 pKa = 11.84NGDD122 pKa = 3.74FSQNNQGYY130 pKa = 8.85LVNTSGYY137 pKa = 8.75YY138 pKa = 10.2LEE140 pKa = 5.6GYY142 pKa = 9.3QVGSEE147 pKa = 4.28GALSSTLSPIKK158 pKa = 10.46ISDD161 pKa = 3.66SVAFQPTKK169 pKa = 9.97STTVSLSAAIGSTSGAEE186 pKa = 4.13GTNTSTATAYY196 pKa = 10.24DD197 pKa = 3.74SKK199 pKa = 11.83GNAQEE204 pKa = 5.22VSLNWKK210 pKa = 9.79QSSTDD215 pKa = 3.5PLVWTVSNSADD226 pKa = 3.33SSNSATVKK234 pKa = 10.45FNSDD238 pKa = 2.67GSLASVNGASQSTGSAATFSYY259 pKa = 10.26TGLPQDD265 pKa = 3.36MTVNLGTIGSTSGVSLATDD284 pKa = 3.57SSNYY288 pKa = 7.99STNPTMTTDD297 pKa = 3.31SVTSGTFTGLSMQSDD312 pKa = 4.64GSVMATFDD320 pKa = 3.57NGLSQLLAKK329 pKa = 10.27IPLSTFADD337 pKa = 3.94PNGLSAQNGQAYY349 pKa = 6.92TATASSGAPTVNAVNTNGAGTLSTSSTEE377 pKa = 4.18SSTTDD382 pKa = 3.25LTSDD386 pKa = 3.46LTKK389 pKa = 10.96LIVAQQAYY397 pKa = 9.02GANTKK402 pKa = 9.86IVTTANQLLQTTLAMIQQ419 pKa = 3.18

Molecular weight: 42.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A252BPG3|A0A252BPG3_9PROT Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase OS=Gluconobacter sp. DsW_058 OX=1511210 GN=HK24_06550 PE=3 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84ALLVCLFLTVTTLMTPDD19 pKa = 4.26ASAQGLEE26 pKa = 4.06SGMLSVRR33 pKa = 11.84MRR35 pKa = 11.84LAEE38 pKa = 4.28DD39 pKa = 3.29AGMSGVDD46 pKa = 3.48RR47 pKa = 11.84RR48 pKa = 11.84LIVCRR53 pKa = 11.84INPRR57 pKa = 11.84LLHH60 pKa = 5.52TVWVPNVKK68 pKa = 10.01EE69 pKa = 3.81PRR71 pKa = 11.84QPQVCTGGANRR82 pKa = 11.84LGAGYY87 pKa = 10.33VRR89 pKa = 11.84YY90 pKa = 9.89LSTSRR95 pKa = 11.84VSKK98 pKa = 10.84AEE100 pKa = 3.75KK101 pKa = 9.61PLRR104 pKa = 11.84GG105 pKa = 3.48

Molecular weight: 11.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2448

0

2448

805449

31

2117

329.0

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.081 ± 0.057

0.946 ± 0.015

5.558 ± 0.038

5.233 ± 0.044

3.566 ± 0.03

8.366 ± 0.047

2.453 ± 0.022

5.017 ± 0.034

3.189 ± 0.036

10.444 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.018

2.928 ± 0.034

5.404 ± 0.038

3.665 ± 0.03

6.638 ± 0.044

6.285 ± 0.04

5.908 ± 0.035

7.121 ± 0.039

1.388 ± 0.02

2.318 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski