Lachnoclostridium sp. An118

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium; unclassified Lachnoclostridium

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4U3U2|A0A1Y4U3U2_9FIRM 50S ribosomal protein L20 OS=Lachnoclostridium sp. An118 OX=1965547 GN=rplT PE=3 SV=1
MM1 pKa = 7.98DD2 pKa = 5.9KK3 pKa = 10.39IKK5 pKa = 10.9HH6 pKa = 5.66SFNSIASKK14 pKa = 10.12QGSYY18 pKa = 10.88SVGLTALVIAIVVLINLIVGQLPSQIRR45 pKa = 11.84QIDD48 pKa = 3.23ISTNNIYY55 pKa = 10.59EE56 pKa = 3.98ITDD59 pKa = 3.39TSRR62 pKa = 11.84EE63 pKa = 4.1LLDD66 pKa = 4.11GLDD69 pKa = 3.43RR70 pKa = 11.84DD71 pKa = 4.02VTFTVLADD79 pKa = 3.56KK80 pKa = 11.06SSTDD84 pKa = 3.26EE85 pKa = 4.74RR86 pKa = 11.84ISNFIDD92 pKa = 3.74RR93 pKa = 11.84YY94 pKa = 9.66SALSSHH100 pKa = 6.96INVEE104 pKa = 4.01WIDD107 pKa = 3.95PVLHH111 pKa = 6.6PSALTTYY118 pKa = 10.42SADD121 pKa = 2.98SDD123 pKa = 4.57TIVVSCDD130 pKa = 2.98DD131 pKa = 3.9TDD133 pKa = 4.01RR134 pKa = 11.84STTVAFSDD142 pKa = 3.73IITYY146 pKa = 10.95DD147 pKa = 3.1EE148 pKa = 3.9MSYY151 pKa = 9.28YY152 pKa = 7.97TTGSATEE159 pKa = 4.24SEE161 pKa = 4.48FDD163 pKa = 4.43GEE165 pKa = 4.66GQLSSAVNYY174 pKa = 7.29VTTDD178 pKa = 3.04VTRR181 pKa = 11.84NVYY184 pKa = 8.17YY185 pKa = 10.43TSGHH189 pKa = 6.04GEE191 pKa = 4.05SSLSSSVTDD200 pKa = 3.97LLGKK204 pKa = 10.44SSLTLTEE211 pKa = 4.87LNLMMASSIPEE222 pKa = 4.34DD223 pKa = 3.98CDD225 pKa = 4.14LLLMDD230 pKa = 5.69APANDD235 pKa = 3.71LSEE238 pKa = 6.09DD239 pKa = 3.99EE240 pKa = 5.04VSMLTDD246 pKa = 3.29YY247 pKa = 11.02MDD249 pKa = 4.14GGGHH253 pKa = 7.21LFVILGSDD261 pKa = 3.89VSQTPNLASFLEE273 pKa = 4.93TYY275 pKa = 10.83GLTSAGGYY283 pKa = 8.31IADD286 pKa = 4.13MQRR289 pKa = 11.84SYY291 pKa = 11.07QGNYY295 pKa = 9.74YY296 pKa = 10.48YY297 pKa = 10.05IFPEE301 pKa = 4.25LSVSGDD307 pKa = 3.48MANEE311 pKa = 4.05LQSDD315 pKa = 3.92MVLLVNANGLVEE327 pKa = 4.24GTPSRR332 pKa = 11.84DD333 pKa = 3.65TITLTPFMTTSSSGYY348 pKa = 10.52AVTEE352 pKa = 4.8DD353 pKa = 3.7GQSTQGTYY361 pKa = 11.1VLGATATEE369 pKa = 3.94NDD371 pKa = 3.18SRR373 pKa = 11.84LTVISSEE380 pKa = 4.23SMIDD384 pKa = 3.36EE385 pKa = 5.09TITSQFTNIEE395 pKa = 3.96NLTLFMNAVTANFDD409 pKa = 4.29DD410 pKa = 4.32IEE412 pKa = 4.31NLSIEE417 pKa = 4.39PKK419 pKa = 10.35SLQIEE424 pKa = 4.81YY425 pKa = 8.42NTMQYY430 pKa = 9.99TGLIGLAAIVGIPVVFLLYY449 pKa = 10.73GLRR452 pKa = 11.84RR453 pKa = 11.84WMKK456 pKa = 9.67RR457 pKa = 11.84RR458 pKa = 11.84KK459 pKa = 9.4AA460 pKa = 3.44

Molecular weight: 50.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4TVF3|A0A1Y4TVF3_9FIRM Uncharacterized protein OS=Lachnoclostridium sp. An118 OX=1965547 GN=B5E62_12990 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.2VHH16 pKa = 5.65GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3184

0

3184

1015018

35

5317

318.8

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.929 ± 0.047

1.535 ± 0.018

5.694 ± 0.036

8.048 ± 0.049

3.965 ± 0.029

7.606 ± 0.041

1.762 ± 0.021

6.645 ± 0.041

5.855 ± 0.045

9.238 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.091 ± 0.025

3.495 ± 0.032

3.492 ± 0.026

3.331 ± 0.024

5.592 ± 0.047

5.593 ± 0.035

5.271 ± 0.04

6.955 ± 0.037

0.926 ± 0.015

3.979 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski