Halovirus HGTV-1
Average proteome isoelectric point is 5.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 281 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4T6P3|R4T6P3_9VIRU PD-(D/E)XK nuclease OS=Halovirus HGTV-1 OX=1273749 GN=39 PE=4 SV=1
MM1 pKa = 7.82 AYY3 pKa = 10.16 DD4 pKa = 3.71 PQYY7 pKa = 11.78 VSIDD11 pKa = 3.79 DD12 pKa = 4.19 VPVQIPDD19 pKa = 3.91 DD20 pKa = 4.01 YY21 pKa = 11.68 SPSEE25 pKa = 4.21 KK26 pKa = 10.28 EE27 pKa = 3.96 DD28 pKa = 3.35 ALEE31 pKa = 3.88 YY32 pKa = 11.26 AEE34 pKa = 5.94 ASLEE38 pKa = 4.14 LDD40 pKa = 4.29 LNDD43 pKa = 4.01 GAAIPSGSVSAMMKK57 pKa = 10.37 AAVKK61 pKa = 10.2 QLATCQLAKK70 pKa = 10.45 GAEE73 pKa = 4.18 HH74 pKa = 7.47 PDD76 pKa = 4.48 DD77 pKa = 3.72 ITLGDD82 pKa = 4.42 LNDD85 pKa = 3.93 TGDD88 pKa = 3.77 TKK90 pKa = 11.12 VSYY93 pKa = 11.08 AEE95 pKa = 4.31 SFCDD99 pKa = 3.67 EE100 pKa = 4.3 YY101 pKa = 11.58 EE102 pKa = 4.52 KK103 pKa = 10.82 IVDD106 pKa = 3.62 KK107 pKa = 11.02 LVNSGALEE115 pKa = 4.13 DD116 pKa = 4.9 LEE118 pKa = 5.99 PDD120 pKa = 3.76 GQSTSPYY127 pKa = 10.34 VYY129 pKa = 9.7 NTDD132 pKa = 4.24 DD133 pKa = 3.76 PTPGNRR139 pKa = 11.84 WSS141 pKa = 3.31
Molecular weight: 15.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.681
IPC_protein 3.681
Toseland 3.452
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.503
Grimsley 3.363
Solomon 3.668
Lehninger 3.617
Nozaki 3.795
DTASelect 4.037
Thurlkill 3.516
EMBOSS 3.63
Sillero 3.795
Patrickios 0.985
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|R4TGP3|R4TGP3_9VIRU Uncharacterized protein OS=Halovirus HGTV-1 OX=1273749 GN=99 PE=4 SV=1
MM1 pKa = 8.07 RR2 pKa = 11.84 DD3 pKa = 2.84 RR4 pKa = 11.84 GRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 SVRR11 pKa = 11.84 IGSVRR16 pKa = 11.84 IVGGTSSGIGGLSITGFRR34 pKa = 11.84 LLVQRR39 pKa = 11.84 RR40 pKa = 11.84 IGFTSFAIIVSLRR53 pKa = 11.84 RR54 pKa = 11.84 TRR56 pKa = 11.84 KK57 pKa = 9.38 FEE59 pKa = 4.12 SGNRR63 pKa = 11.84 NLYY66 pKa = 8.38 ITTTRR71 pKa = 11.84 FQSVV75 pKa = 2.76
Molecular weight: 8.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 10.804
IPC_protein 12.281
Toseland 12.442
ProMoST 12.939
Dawson 12.442
Bjellqvist 12.442
Wikipedia 12.925
Rodwell 11.974
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.442
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.711
IPC_peptide 12.939
IPC2_peptide 11.93
IPC2.peptide.svr19 9.183
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
281
0
281
42727
26
983
152.1
17.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.956 ± 0.243
1.039 ± 0.085
8.21 ± 0.188
9.945 ± 0.284
3.824 ± 0.122
7.183 ± 0.163
2.263 ± 0.102
4.908 ± 0.12
4.517 ± 0.145
6.963 ± 0.16
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.881 ± 0.105
4.313 ± 0.113
3.803 ± 0.105
3.27 ± 0.091
5.549 ± 0.154
6.385 ± 0.154
5.547 ± 0.173
7.092 ± 0.138
1.533 ± 0.066
3.82 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here