Erwinia sp. OLSSP12

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Erwinia; unclassified Erwinia

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3383 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G8EGV9|A0A2G8EGV9_9GAMM OmpR/PhoB-type domain-containing protein OS=Erwinia sp. OLSSP12 OX=1912096 GN=BK416_05855 PE=4 SV=1
MM1 pKa = 7.98SYY3 pKa = 11.22EE4 pKa = 4.04EE5 pKa = 4.36LLEE8 pKa = 4.09EE9 pKa = 4.17QRR11 pKa = 11.84EE12 pKa = 4.18EE13 pKa = 3.67TRR15 pKa = 11.84LIIDD19 pKa = 3.94EE20 pKa = 4.4LLEE23 pKa = 5.24DD24 pKa = 5.0GSDD27 pKa = 3.46PDD29 pKa = 3.46ALYY32 pKa = 9.93TIEE35 pKa = 4.77HH36 pKa = 6.75HH37 pKa = 6.84LSCANFDD44 pKa = 3.97ALEE47 pKa = 4.28KK48 pKa = 10.54VAVEE52 pKa = 4.15AFRR55 pKa = 11.84LGYY58 pKa = 10.25EE59 pKa = 4.01VTDD62 pKa = 3.98PEE64 pKa = 4.3EE65 pKa = 5.63LEE67 pKa = 5.16LEE69 pKa = 4.66DD70 pKa = 4.45GSKK73 pKa = 11.04VMCCDD78 pKa = 4.05IISEE82 pKa = 4.3LALNAALLDD91 pKa = 3.97EE92 pKa = 4.52QVEE95 pKa = 4.29QLVKK99 pKa = 10.66LAGKK103 pKa = 10.19FNVDD107 pKa = 2.85YY108 pKa = 10.7DD109 pKa = 3.37GWGTYY114 pKa = 10.12FEE116 pKa = 5.56DD117 pKa = 4.97PNAEE121 pKa = 4.4DD122 pKa = 4.13PQSDD126 pKa = 4.1DD127 pKa = 5.25DD128 pKa = 5.82YY129 pKa = 11.92VDD131 pKa = 4.04EE132 pKa = 6.24DD133 pKa = 4.64DD134 pKa = 5.86DD135 pKa = 4.65GVRR138 pKa = 11.84HH139 pKa = 6.05

Molecular weight: 15.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G8E5D7|A0A2G8E5D7_9GAMM UTP--glucose-1-phosphate uridylyltransferase OS=Erwinia sp. OLSSP12 OX=1912096 GN=BK416_14110 PE=3 SV=1
RR1 pKa = 7.86VLTMAVNLNRR11 pKa = 11.84LTTGTMNVNAGHH23 pKa = 6.64KK24 pKa = 7.69RR25 pKa = 11.84TSKK28 pKa = 10.78ARR30 pKa = 11.84IIHH33 pKa = 5.9QIQPIRR39 pKa = 11.84GITRR43 pKa = 3.65

Molecular weight: 4.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3383

0

3383

1038597

34

1725

307.0

33.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.099 ± 0.051

1.102 ± 0.015

5.164 ± 0.033

5.132 ± 0.043

3.727 ± 0.028

7.32 ± 0.04

2.364 ± 0.022

5.702 ± 0.037

3.921 ± 0.038

11.114 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.021

3.684 ± 0.031

4.557 ± 0.029

5.061 ± 0.046

6.103 ± 0.039

6.153 ± 0.034

5.166 ± 0.033

6.747 ± 0.033

1.501 ± 0.02

2.75 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski