Gulosibacter molinativorax
Average proteome isoelectric point is 5.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3115 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P7AFA5|A0A2P7AFA5_9MICO DUF3093 domain-containing protein OS=Gulosibacter molinativorax OX=256821 GN=GMOLON4_07830 PE=4 SV=1
MM1 pKa = 6.5 QRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 IANRR9 pKa = 11.84 MWLAGAAVAALLLSGCQQVSQSDD32 pKa = 3.64 QTEE35 pKa = 4.12 VDD37 pKa = 3.33 LGTPVAGEE45 pKa = 3.96 VPEE48 pKa = 4.58 GVFEE52 pKa = 4.3 GTTLTFAGSGGIFQDD67 pKa = 3.96 GQAAAAWDD75 pKa = 3.91 PFAEE79 pKa = 4.27 QTGATFLQDD88 pKa = 3.64 AYY90 pKa = 11.21 DD91 pKa = 4.21 PGKK94 pKa = 10.32 LKK96 pKa = 11.27 AMVDD100 pKa = 3.72 SGNTTWDD107 pKa = 3.8 LVNATQFDD115 pKa = 3.9 TAQYY119 pKa = 10.65 CGTLYY124 pKa = 11.07 EE125 pKa = 4.42 EE126 pKa = 5.32 LDD128 pKa = 3.64 YY129 pKa = 11.67 SQIDD133 pKa = 3.46 ISNVPEE139 pKa = 3.93 GTITDD144 pKa = 3.83 DD145 pKa = 4.46 CMVPQILYY153 pKa = 11.0 GLVLVYY159 pKa = 9.97 NTDD162 pKa = 3.32 TFGDD166 pKa = 3.95 NPPTTAADD174 pKa = 3.83 FFDD177 pKa = 4.0 TEE179 pKa = 4.35 KK180 pKa = 11.01 FPGQRR185 pKa = 11.84 TVSASPYY192 pKa = 9.66 VDD194 pKa = 2.97 PQMFEE199 pKa = 4.13 FAMVADD205 pKa = 5.12 DD206 pKa = 5.15 KK207 pKa = 11.03 DD208 pKa = 4.12 TSSVTADD215 pKa = 4.55 DD216 pKa = 4.36 IVPALDD222 pKa = 3.38 KK223 pKa = 11.07 YY224 pKa = 11.17 RR225 pKa = 11.84 EE226 pKa = 4.22 IEE228 pKa = 4.21 DD229 pKa = 4.77 SLITWTSGAQAQQQLEE245 pKa = 4.25 SGEE248 pKa = 4.17 AVMGLVWSGRR258 pKa = 11.84 GYY260 pKa = 10.74 GAANAGAPVAAMWDD274 pKa = 3.34 EE275 pKa = 3.92 WMVMVDD281 pKa = 3.08 STAIPKK287 pKa = 10.34 GSQNLQAAHH296 pKa = 6.16 SAINYY301 pKa = 9.21 YY302 pKa = 10.51 GGAEE306 pKa = 4.1 QQTKK310 pKa = 9.23 MSEE313 pKa = 4.05 LTSYY317 pKa = 11.46 GPINVNAKK325 pKa = 9.52 PDD327 pKa = 3.59 LSGEE331 pKa = 4.15 LGNWMTTDD339 pKa = 3.44 HH340 pKa = 7.63 LDD342 pKa = 3.32 TGHH345 pKa = 6.21 YY346 pKa = 9.53 PNVDD350 pKa = 3.81 FWVEE354 pKa = 3.96 NYY356 pKa = 9.18 DD357 pKa = 3.77 ALQSAWSSWVTTGG370 pKa = 4.48
Molecular weight: 39.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.617
EMBOSS 3.719
Sillero 3.91
Patrickios 1.252
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A2P7AEK4|A0A2P7AEK4_9MICO Type II toxin-antitoxin system RelE/ParE family toxin OS=Gulosibacter molinativorax OX=256821 GN=GMOLON4_08800 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SKK15 pKa = 10.39 KK16 pKa = 9.03 HH17 pKa = 4.36 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILASRR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.36 GRR40 pKa = 11.84 KK41 pKa = 8.53 VLSAA45 pKa = 4.05
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3115
0
3115
1006633
29
2245
323.2
34.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.119 ± 0.066
0.487 ± 0.01
6.023 ± 0.038
6.676 ± 0.046
3.278 ± 0.024
8.524 ± 0.04
2.032 ± 0.022
5.135 ± 0.033
2.578 ± 0.03
9.779 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.994 ± 0.018
2.524 ± 0.025
5.006 ± 0.029
3.249 ± 0.03
6.894 ± 0.05
5.904 ± 0.032
6.041 ± 0.033
8.219 ± 0.04
1.436 ± 0.016
2.103 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here