Capybara microvirus Cap3_SP_450
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVT8|A0A4V1FVT8_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_450 OX=2585460 PE=4 SV=1
MM1 pKa = 7.7 KK2 pKa = 10.07 KK3 pKa = 10.05 QKK5 pKa = 10.22 LFKK8 pKa = 10.51 GKK10 pKa = 9.26 EE11 pKa = 3.84 IKK13 pKa = 10.34 VEE15 pKa = 4.36 RR16 pKa = 11.84 ISEE19 pKa = 4.03 KK20 pKa = 10.23 EE21 pKa = 4.13 MYY23 pKa = 9.15 WGIPYY28 pKa = 10.34 GIYY31 pKa = 10.03 KK32 pKa = 9.63 EE33 pKa = 4.0 RR34 pKa = 11.84 EE35 pKa = 3.94 YY36 pKa = 11.13 YY37 pKa = 8.71 YY38 pKa = 10.93 TDD40 pKa = 4.03 LDD42 pKa = 4.24 DD43 pKa = 4.37 NGRR46 pKa = 11.84 EE47 pKa = 4.04 IGNMEE52 pKa = 4.03 YY53 pKa = 11.05 AEE55 pKa = 4.22 NDD57 pKa = 3.06 EE58 pKa = 4.7 EE59 pKa = 5.44 GIKK62 pKa = 10.32 KK63 pKa = 10.12 CIKK66 pKa = 10.31 NLEE69 pKa = 4.16 CLEE72 pKa = 4.83 EE73 pKa = 3.96 INKK76 pKa = 10.22 YY77 pKa = 10.42 YY78 pKa = 10.47 EE79 pKa = 3.81
Molecular weight: 9.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.909
IPC2_protein 5.08
IPC_protein 4.902
Toseland 4.774
ProMoST 4.863
Dawson 4.813
Bjellqvist 5.003
Wikipedia 4.622
Rodwell 4.736
Grimsley 4.685
Solomon 4.813
Lehninger 4.762
Nozaki 4.914
DTASelect 4.978
Thurlkill 4.749
EMBOSS 4.647
Sillero 5.003
Patrickios 3.719
IPC_peptide 4.825
IPC2_peptide 4.991
IPC2.peptide.svr19 5.038
Protein with the highest isoelectric point:
>tr|A0A4P8W580|A0A4P8W580_9VIRU Nonstructural protein OS=Capybara microvirus Cap3_SP_450 OX=2585460 PE=4 SV=1
MM1 pKa = 7.31 NKK3 pKa = 9.92 KK4 pKa = 9.94 IPWRR8 pKa = 11.84 EE9 pKa = 3.94 SEE11 pKa = 4.21 FKK13 pKa = 10.58 NWSTKK18 pKa = 7.65 EE19 pKa = 3.77 FEE21 pKa = 4.3 EE22 pKa = 4.6 FFLIKK27 pKa = 10.24 KK28 pKa = 8.35 RR29 pKa = 11.84 TVEE32 pKa = 3.76 NYY34 pKa = 9.16 RR35 pKa = 11.84 YY36 pKa = 10.02 RR37 pKa = 11.84 GIPEE41 pKa = 4.05 EE42 pKa = 4.18 KK43 pKa = 10.11 YY44 pKa = 10.74 KK45 pKa = 10.58 ILIYY49 pKa = 10.12 ILKK52 pKa = 9.76 LKK54 pKa = 11.01 NEE56 pKa = 3.95 IKK58 pKa = 10.34 EE59 pKa = 4.16 RR60 pKa = 11.84 EE61 pKa = 4.37 HH62 pKa = 8.97 NDD64 pKa = 2.89 MLQSKK69 pKa = 9.45 NKK71 pKa = 9.74 NRR73 pKa = 11.84 II74 pKa = 3.29
Molecular weight: 9.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.945
IPC2_protein 9.121
IPC_protein 9.048
Toseland 9.97
ProMoST 9.545
Dawson 10.131
Bjellqvist 9.736
Wikipedia 10.248
Rodwell 10.73
Grimsley 10.175
Solomon 10.16
Lehninger 10.131
Nozaki 9.926
DTASelect 9.736
Thurlkill 9.984
EMBOSS 10.35
Sillero 10.028
Patrickios 10.452
IPC_peptide 10.16
IPC2_peptide 8.112
IPC2.peptide.svr19 8.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1541
74
542
220.1
25.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.852 ± 1.93
1.038 ± 0.379
4.478 ± 0.778
8.955 ± 1.984
3.05 ± 0.397
6.684 ± 0.767
1.947 ± 0.413
7.592 ± 0.666
8.436 ± 2.047
6.684 ± 0.397
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.596 ± 0.382
5.97 ± 0.901
3.764 ± 0.791
4.153 ± 0.98
4.348 ± 0.673
5.97 ± 1.014
6.035 ± 0.866
3.18 ± 0.558
1.817 ± 0.234
5.451 ± 0.816
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here