Ralstonia phage RSS1

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Restivirus; Ralstonia virus RSS1

Average proteome isoelectric point is 7.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0JC27|A0JC27_9VIRU Uncharacterized protein OS=Ralstonia phage RSS1 OX=384360 PE=4 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84VLVLLLCWWASSAWASTIPLIPPPNIVLTGTGYY35 pKa = 8.68VTTGAVTLSEE45 pKa = 4.26VATATEE51 pKa = 3.73MRR53 pKa = 11.84AAVGAGAATIAATMTVGEE71 pKa = 4.37GAAAVALAALRR82 pKa = 11.84ATPAIATATSLAYY95 pKa = 10.22LAQLGIQKK103 pKa = 10.22CLDD106 pKa = 3.79GTWCTSKK113 pKa = 10.67RR114 pKa = 11.84SPAAGDD120 pKa = 3.22TGFNGWGWTYY130 pKa = 11.08GYY132 pKa = 7.89NTSATGGNIANGVAASPGAACSAMLAADD160 pKa = 5.01AYY162 pKa = 11.27LAGQKK167 pKa = 10.68AKK169 pKa = 10.24FAGMKK174 pKa = 9.0ATGNGTSYY182 pKa = 10.51EE183 pKa = 4.07CHH185 pKa = 5.33YY186 pKa = 10.2TNDD189 pKa = 3.75GGDD192 pKa = 3.15NFYY195 pKa = 11.16AGTSQASSCVDD206 pKa = 3.64GYY208 pKa = 10.97VVSGSACVPDD218 pKa = 3.98PAGPTVGASDD228 pKa = 4.38ADD230 pKa = 3.82WNKK233 pKa = 10.46GLTYY237 pKa = 9.63PLPAGVASDD246 pKa = 3.39MAAAKK251 pKa = 10.52VPIPVKK257 pKa = 9.49LTPSTTPVNVNLSDD271 pKa = 4.25PYY273 pKa = 11.06VDD275 pKa = 3.41PVTGKK280 pKa = 10.3RR281 pKa = 11.84YY282 pKa = 9.86RR283 pKa = 11.84DD284 pKa = 3.59VATVTPNSDD293 pKa = 2.78GKK295 pKa = 8.9TATLTTGKK303 pKa = 10.15QEE305 pKa = 3.53VDD307 pKa = 3.25ANGNPATDD315 pKa = 3.73PATGNGKK322 pKa = 9.89APEE325 pKa = 4.29KK326 pKa = 10.6QDD328 pKa = 3.76DD329 pKa = 3.81QCSGHH334 pKa = 5.4EE335 pKa = 4.06TRR337 pKa = 11.84MGCIEE342 pKa = 4.1QGEE345 pKa = 4.53IPDD348 pKa = 4.33GPDD351 pKa = 3.42LKK353 pKa = 10.65EE354 pKa = 3.58QQVNVKK360 pKa = 7.96VTPDD364 pKa = 3.69SGWGADD370 pKa = 3.69TAPCPSDD377 pKa = 3.24LTASIHH383 pKa = 5.69GMPISWSLKK392 pKa = 8.3PVCDD396 pKa = 3.58GADD399 pKa = 3.03MFRR402 pKa = 11.84PVIIACAWLGAALIVIGVGRR422 pKa = 11.84KK423 pKa = 9.67GEE425 pKa = 4.05EE426 pKa = 3.72

Molecular weight: 43.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0JC31|A0JC31_9VIRU Uncharacterized protein OS=Ralstonia phage RSS1 OX=384360 PE=4 SV=1
MM1 pKa = 6.98TRR3 pKa = 11.84YY4 pKa = 9.54VHH6 pKa = 6.68TGPPTYY12 pKa = 10.34KK13 pKa = 10.34HH14 pKa = 6.42PLSCKK19 pKa = 9.57PAFEE23 pKa = 4.24TLTIFGCAGCGGSLRR38 pKa = 11.84VPMLRR43 pKa = 11.84CSEE46 pKa = 4.14QPKK49 pKa = 10.4SAVSDD54 pKa = 4.15DD55 pKa = 3.62EE56 pKa = 4.5LKK58 pKa = 11.0AVARR62 pKa = 11.84EE63 pKa = 3.67IRR65 pKa = 11.84HH66 pKa = 5.95LYY68 pKa = 7.49WHH70 pKa = 6.76IRR72 pKa = 11.84TLRR75 pKa = 11.84RR76 pKa = 11.84GYY78 pKa = 10.46QDD80 pKa = 3.34AARR83 pKa = 11.84HH84 pKa = 5.24RR85 pKa = 11.84YY86 pKa = 7.58YY87 pKa = 10.94RR88 pKa = 11.84KK89 pKa = 9.43IRR91 pKa = 11.84TKK93 pKa = 10.29KK94 pKa = 10.14KK95 pKa = 10.21RR96 pKa = 11.84LLEE99 pKa = 4.03YY100 pKa = 10.81NRR102 pKa = 11.84LTRR105 pKa = 11.84ARR107 pKa = 11.84GKK109 pKa = 10.35

Molecular weight: 12.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

2113

26

426

176.1

19.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.92 ± 0.978

1.656 ± 0.285

5.111 ± 0.488

5.111 ± 0.883

2.508 ± 0.486

8.566 ± 0.881

1.609 ± 0.343

4.354 ± 0.504

5.111 ± 0.549

7.903 ± 0.766

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.982 ± 0.304

1.988 ± 0.463

6.01 ± 0.88

3.881 ± 0.559

6.484 ± 1.224

5.348 ± 0.49

6.72 ± 0.827

7.383 ± 0.361

1.704 ± 0.322

2.65 ± 0.326
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski