Amino acid dipepetide frequency for Ralstonia phage RSS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.833AlaAla: 20.833 ± 4.104
3.788AlaCys: 3.788 ± 1.193
5.208AlaAsp: 5.208 ± 1.947
6.629AlaGlu: 6.629 ± 2.449
3.788AlaPhe: 3.788 ± 1.148
12.311AlaGly: 12.311 ± 2.338
1.42AlaHis: 1.42 ± 1.007
4.261AlaIle: 4.261 ± 1.376
3.314AlaLys: 3.314 ± 0.692
9.47AlaLeu: 9.47 ± 2.446
5.682AlaMet: 5.682 ± 1.979
2.367AlaAsn: 2.367 ± 0.763
3.788AlaPro: 3.788 ± 2.016
5.682AlaGln: 5.682 ± 2.17
7.576AlaArg: 7.576 ± 2.466
4.735AlaSer: 4.735 ± 2.337
10.417AlaThr: 10.417 ± 4.463
11.364AlaVal: 11.364 ± 2.348
2.367AlaTrp: 2.367 ± 0.942
4.261AlaTyr: 4.261 ± 0.906
0.0AlaXaa: 0.0 ± 0.0
Cys
1.42CysAla: 1.42 ± 0.617
0.473CysCys: 0.473 ± 0.485
1.42CysAsp: 1.42 ± 0.701
0.473CysGlu: 0.473 ± 0.491
0.473CysPhe: 0.473 ± 0.548
0.947CysGly: 0.947 ± 0.611
0.473CysHis: 0.473 ± 0.34
0.473CysIle: 0.473 ± 0.34
0.473CysLys: 0.473 ± 0.525
0.947CysLeu: 0.947 ± 0.58
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.473CysPro: 0.473 ± 0.34
0.947CysGln: 0.947 ± 0.516
0.947CysArg: 0.947 ± 0.467
1.42CysSer: 1.42 ± 0.757
2.841CysThr: 2.841 ± 0.937
1.42CysVal: 1.42 ± 0.723
0.473CysTrp: 0.473 ± 0.34
0.473CysTyr: 0.473 ± 0.418
0.0CysXaa: 0.0 ± 0.0
Asp
6.629AspAla: 6.629 ± 0.968
0.473AspCys: 0.473 ± 0.445
2.367AspAsp: 2.367 ± 0.772
2.367AspGlu: 2.367 ± 0.799
1.42AspPhe: 1.42 ± 0.888
6.155AspGly: 6.155 ± 2.351
0.947AspHis: 0.947 ± 0.596
2.367AspIle: 2.367 ± 1.328
0.947AspLys: 0.947 ± 0.63
4.261AspLeu: 4.261 ± 1.486
1.42AspMet: 1.42 ± 0.723
1.42AspAsn: 1.42 ± 0.885
4.735AspPro: 4.735 ± 1.203
1.894AspGln: 1.894 ± 0.667
1.894AspArg: 1.894 ± 1.115
2.367AspSer: 2.367 ± 0.63
1.894AspThr: 1.894 ± 0.839
4.261AspVal: 4.261 ± 1.925
2.841AspTrp: 2.841 ± 0.987
0.473AspTyr: 0.473 ± 0.374
0.0AspXaa: 0.0 ± 0.0
Glu
1.894GluAla: 1.894 ± 0.897
0.947GluCys: 0.947 ± 0.483
1.894GluAsp: 1.894 ± 0.817
3.314GluGlu: 3.314 ± 1.346
1.894GluPhe: 1.894 ± 1.008
1.894GluGly: 1.894 ± 0.651
0.473GluHis: 0.473 ± 0.374
3.788GluIle: 3.788 ± 1.26
2.841GluLys: 2.841 ± 0.867
5.208GluLeu: 5.208 ± 1.654
2.367GluMet: 2.367 ± 0.804
0.947GluAsn: 0.947 ± 0.467
1.894GluPro: 1.894 ± 0.969
6.629GluGln: 6.629 ± 2.773
2.367GluArg: 2.367 ± 1.212
2.367GluSer: 2.367 ± 0.95
4.735GluThr: 4.735 ± 1.083
3.788GluVal: 3.788 ± 1.268
1.42GluTrp: 1.42 ± 0.802
0.473GluTyr: 0.473 ± 0.525
0.0GluXaa: 0.0 ± 0.0
Phe
1.894PheAla: 1.894 ± 0.674
0.473PheCys: 0.473 ± 0.374
0.473PheAsp: 0.473 ± 0.418
1.894PheGlu: 1.894 ± 0.718
0.0PhePhe: 0.0 ± 0.0
1.894PheGly: 1.894 ± 0.829
0.473PheHis: 0.473 ± 0.374
1.42PheIle: 1.42 ± 0.682
1.894PheLys: 1.894 ± 1.284
2.841PheLeu: 2.841 ± 1.046
0.473PheMet: 0.473 ± 0.374
1.42PheAsn: 1.42 ± 0.446
1.894PhePro: 1.894 ± 0.859
0.0PheGln: 0.0 ± 0.0
2.367PheArg: 2.367 ± 0.626
0.947PheSer: 0.947 ± 0.749
1.42PheThr: 1.42 ± 0.578
1.894PheVal: 1.894 ± 1.232
0.473PheTrp: 0.473 ± 0.592
0.947PheTyr: 0.947 ± 0.556
0.0PheXaa: 0.0 ± 0.0
Gly
10.89GlyAla: 10.89 ± 3.247
2.367GlyCys: 2.367 ± 1.136
2.367GlyAsp: 2.367 ± 0.981
3.788GlyGlu: 3.788 ± 1.544
1.894GlyPhe: 1.894 ± 0.674
6.629GlyGly: 6.629 ± 1.415
0.473GlyHis: 0.473 ± 0.34
3.788GlyIle: 3.788 ± 1.134
6.629GlyLys: 6.629 ± 1.671
5.208GlyLeu: 5.208 ± 1.845
4.261GlyMet: 4.261 ± 1.231
3.788GlyAsn: 3.788 ± 1.278
3.788GlyPro: 3.788 ± 0.91
2.367GlyGln: 2.367 ± 0.844
6.629GlyArg: 6.629 ± 1.751
4.261GlySer: 4.261 ± 1.239
5.682GlyThr: 5.682 ± 1.289
6.629GlyVal: 6.629 ± 1.211
2.367GlyTrp: 2.367 ± 1.593
1.894GlyTyr: 1.894 ± 1.033
0.0GlyXaa: 0.0 ± 0.0
His
0.473HisAla: 0.473 ± 0.445
0.0HisCys: 0.0 ± 0.0
0.473HisAsp: 0.473 ± 0.374
0.947HisGlu: 0.947 ± 0.422
0.0HisPhe: 0.0 ± 0.0
1.42HisGly: 1.42 ± 0.648
0.0HisHis: 0.0 ± 0.0
2.367HisIle: 2.367 ± 0.911
0.473HisLys: 0.473 ± 0.374
2.841HisLeu: 2.841 ± 0.97
0.0HisMet: 0.0 ± 0.0
0.947HisAsn: 0.947 ± 0.63
0.947HisPro: 0.947 ± 0.697
0.0HisGln: 0.0 ± 0.0
1.894HisArg: 1.894 ± 1.254
0.0HisSer: 0.0 ± 0.0
0.947HisThr: 0.947 ± 0.637
1.894HisVal: 1.894 ± 0.933
0.0HisTrp: 0.0 ± 0.0
0.473HisTyr: 0.473 ± 0.34
0.0HisXaa: 0.0 ± 0.0
Ile
7.576IleAla: 7.576 ± 1.004
0.0IleCys: 0.0 ± 0.0
1.42IleAsp: 1.42 ± 1.052
1.894IleGlu: 1.894 ± 0.815
0.947IlePhe: 0.947 ± 0.779
3.314IleGly: 3.314 ± 0.889
0.947IleHis: 0.947 ± 0.514
2.367IleIle: 2.367 ± 1.076
2.367IleLys: 2.367 ± 0.908
2.367IleLeu: 2.367 ± 0.818
0.0IleMet: 0.0 ± 0.0
0.473IleAsn: 0.473 ± 0.374
3.788IlePro: 3.788 ± 1.283
0.947IleGln: 0.947 ± 0.422
5.208IleArg: 5.208 ± 1.943
2.367IleSer: 2.367 ± 0.624
3.314IleThr: 3.314 ± 1.718
3.314IleVal: 3.314 ± 0.906
0.473IleTrp: 0.473 ± 0.539
0.473IleTyr: 0.473 ± 0.491
0.0IleXaa: 0.0 ± 0.0
Lys
8.049LysAla: 8.049 ± 1.715
0.947LysCys: 0.947 ± 0.514
2.367LysAsp: 2.367 ± 0.977
1.894LysGlu: 1.894 ± 1.224
0.947LysPhe: 0.947 ± 0.422
3.788LysGly: 3.788 ± 1.449
0.947LysHis: 0.947 ± 0.611
1.42LysIle: 1.42 ± 0.849
3.788LysLys: 3.788 ± 1.544
3.314LysLeu: 3.314 ± 0.781
0.473LysMet: 0.473 ± 0.585
0.473LysAsn: 0.473 ± 0.485
4.261LysPro: 4.261 ± 1.363
1.42LysGln: 1.42 ± 0.72
3.788LysArg: 3.788 ± 1.018
4.735LysSer: 4.735 ± 1.593
2.841LysThr: 2.841 ± 0.928
2.841LysVal: 2.841 ± 0.944
0.947LysTrp: 0.947 ± 0.592
1.42LysTyr: 1.42 ± 0.591
0.0LysXaa: 0.0 ± 0.0
Leu
14.205LeuAla: 14.205 ± 3.168
0.473LeuCys: 0.473 ± 0.34
5.682LeuAsp: 5.682 ± 1.096
2.367LeuGlu: 2.367 ± 1.533
0.947LeuPhe: 0.947 ± 0.835
4.735LeuGly: 4.735 ± 1.493
2.367LeuHis: 2.367 ± 0.929
5.208LeuIle: 5.208 ± 1.42
5.208LeuLys: 5.208 ± 0.867
6.629LeuLeu: 6.629 ± 1.975
1.894LeuMet: 1.894 ± 0.886
0.473LeuAsn: 0.473 ± 0.558
4.261LeuPro: 4.261 ± 1.433
2.841LeuGln: 2.841 ± 1.152
6.629LeuArg: 6.629 ± 1.656
4.261LeuSer: 4.261 ± 1.588
4.735LeuThr: 4.735 ± 1.8
4.261LeuVal: 4.261 ± 1.346
0.473LeuTrp: 0.473 ± 0.592
1.42LeuTyr: 1.42 ± 0.637
0.0LeuXaa: 0.0 ± 0.0
Met
4.261MetAla: 4.261 ± 1.569
0.0MetCys: 0.0 ± 0.0
0.947MetAsp: 0.947 ± 0.631
1.894MetGlu: 1.894 ± 0.98
0.947MetPhe: 0.947 ± 0.623
1.894MetGly: 1.894 ± 0.732
0.473MetHis: 0.473 ± 0.485
0.947MetIle: 0.947 ± 0.619
2.367MetLys: 2.367 ± 0.776
3.314MetLeu: 3.314 ± 0.92
1.42MetMet: 1.42 ± 0.948
0.0MetAsn: 0.0 ± 0.0
1.894MetPro: 1.894 ± 0.978
0.947MetGln: 0.947 ± 0.596
3.314MetArg: 3.314 ± 1.414
0.947MetSer: 0.947 ± 0.589
2.367MetThr: 2.367 ± 1.092
1.894MetVal: 1.894 ± 0.758
0.0MetTrp: 0.0 ± 0.0
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
0.947AsnAla: 0.947 ± 0.516
0.0AsnCys: 0.0 ± 0.0
0.473AsnAsp: 0.473 ± 0.34
0.0AsnGlu: 0.0 ± 0.0
0.473AsnPhe: 0.473 ± 0.34
4.735AsnGly: 4.735 ± 1.564
0.0AsnHis: 0.0 ± 0.0
0.947AsnIle: 0.947 ± 0.68
0.473AsnLys: 0.473 ± 0.34
0.947AsnLeu: 0.947 ± 0.623
0.947AsnMet: 0.947 ± 0.63
0.0AsnAsn: 0.0 ± 0.0
2.841AsnPro: 2.841 ± 1.234
1.42AsnGln: 1.42 ± 0.888
0.947AsnArg: 0.947 ± 0.725
1.42AsnSer: 1.42 ± 0.631
1.42AsnThr: 1.42 ± 0.548
1.42AsnVal: 1.42 ± 0.732
0.473AsnTrp: 0.473 ± 0.485
0.0AsnTyr: 0.0 ± 0.0
0.0AsnXaa: 0.0 ± 0.0
Pro
9.943ProAla: 9.943 ± 2.457
0.473ProCys: 0.473 ± 0.34
3.314ProAsp: 3.314 ± 1.138
6.155ProGlu: 6.155 ± 2.573
1.42ProPhe: 1.42 ± 1.058
4.735ProGly: 4.735 ± 0.837
0.947ProHis: 0.947 ± 0.63
2.367ProIle: 2.367 ± 1.3
2.367ProLys: 2.367 ± 1.055
3.314ProLeu: 3.314 ± 1.1
0.947ProMet: 0.947 ± 0.756
1.894ProAsn: 1.894 ± 0.727
2.841ProPro: 2.841 ± 1.291
1.42ProGln: 1.42 ± 0.603
0.473ProArg: 0.473 ± 0.445
4.261ProSer: 4.261 ± 1.11
5.208ProThr: 5.208 ± 1.625
5.682ProVal: 5.682 ± 1.349
0.473ProTrp: 0.473 ± 0.539
1.894ProTyr: 1.894 ± 1.231
0.0ProXaa: 0.0 ± 0.0
Gln
4.261GlnAla: 4.261 ± 1.597
0.947GlnCys: 0.947 ± 0.483
2.367GlnAsp: 2.367 ± 1.1
2.841GlnGlu: 2.841 ± 0.883
0.473GlnPhe: 0.473 ± 0.374
3.314GlnGly: 3.314 ± 1.293
0.473GlnHis: 0.473 ± 0.418
0.473GlnIle: 0.473 ± 0.418
1.894GlnLys: 1.894 ± 0.741
7.102GlnLeu: 7.102 ± 1.8
0.947GlnMet: 0.947 ± 0.467
0.473GlnAsn: 0.473 ± 0.418
2.841GlnPro: 2.841 ± 1.283
0.947GlnGln: 0.947 ± 0.58
3.788GlnArg: 3.788 ± 1.717
0.473GlnSer: 0.473 ± 0.485
0.947GlnThr: 0.947 ± 0.635
2.841GlnVal: 2.841 ± 0.951
0.473GlnTrp: 0.473 ± 0.539
0.947GlnTyr: 0.947 ± 0.636
0.0GlnXaa: 0.0 ± 0.0
Arg
4.261ArgAla: 4.261 ± 1.16
2.367ArgCys: 2.367 ± 0.872
2.841ArgAsp: 2.841 ± 0.829
6.629ArgGlu: 6.629 ± 2.26
0.473ArgPhe: 0.473 ± 0.445
6.155ArgGly: 6.155 ± 2.184
2.841ArgHis: 2.841 ± 1.233
1.894ArgIle: 1.894 ± 0.899
5.208ArgLys: 5.208 ± 1.4
2.841ArgLeu: 2.841 ± 1.32
1.42ArgMet: 1.42 ± 0.745
0.0ArgAsn: 0.0 ± 0.0
3.788ArgPro: 3.788 ± 0.817
3.788ArgGln: 3.788 ± 1.335
5.682ArgArg: 5.682 ± 2.029
4.261ArgSer: 4.261 ± 1.289
3.314ArgThr: 3.314 ± 1.469
4.261ArgVal: 4.261 ± 1.363
0.473ArgTrp: 0.473 ± 0.418
2.367ArgTyr: 2.367 ± 1.128
0.0ArgXaa: 0.0 ± 0.0
Ser
7.576SerAla: 7.576 ± 1.154
0.947SerCys: 0.947 ± 0.555
5.682SerAsp: 5.682 ± 1.395
2.841SerGlu: 2.841 ± 1.003
1.894SerPhe: 1.894 ± 1.035
2.841SerGly: 2.841 ± 0.846
0.473SerHis: 0.473 ± 0.418
2.841SerIle: 2.841 ± 0.949
3.314SerLys: 3.314 ± 1.043
5.682SerLeu: 5.682 ± 1.574
1.42SerMet: 1.42 ± 0.742
1.42SerAsn: 1.42 ± 0.974
2.367SerPro: 2.367 ± 0.871
1.42SerGln: 1.42 ± 0.708
1.894SerArg: 1.894 ± 1.226
4.261SerSer: 4.261 ± 1.219
1.42SerThr: 1.42 ± 0.723
2.367SerVal: 2.367 ± 1.111
1.42SerTrp: 1.42 ± 0.641
1.42SerTyr: 1.42 ± 0.589
0.0SerXaa: 0.0 ± 0.0
Thr
8.523ThrAla: 8.523 ± 3.44
0.947ThrCys: 0.947 ± 0.637
4.735ThrAsp: 4.735 ± 1.075
0.947ThrGlu: 0.947 ± 0.422
3.314ThrPhe: 3.314 ± 1.5
8.996ThrGly: 8.996 ± 2.383
0.473ThrHis: 0.473 ± 0.374
2.841ThrIle: 2.841 ± 0.957
1.42ThrLys: 1.42 ± 1.082
3.788ThrLeu: 3.788 ± 1.261
2.841ThrMet: 2.841 ± 1.034
0.947ThrAsn: 0.947 ± 0.501
6.155ThrPro: 6.155 ± 1.452
1.894ThrGln: 1.894 ± 0.939
2.841ThrArg: 2.841 ± 1.03
4.261ThrSer: 4.261 ± 1.612
2.841ThrThr: 2.841 ± 1.072
5.682ThrVal: 5.682 ± 1.248
0.947ThrTrp: 0.947 ± 0.514
2.841ThrTyr: 2.841 ± 0.894
0.0ThrXaa: 0.0 ± 0.0
Val
10.89ValAla: 10.89 ± 1.869
0.473ValCys: 0.473 ± 0.34
6.155ValAsp: 6.155 ± 1.591
1.42ValGlu: 1.42 ± 1.084
1.894ValPhe: 1.894 ± 0.672
7.102ValGly: 7.102 ± 2.273
1.42ValHis: 1.42 ± 0.637
2.841ValIle: 2.841 ± 0.796
3.788ValLys: 3.788 ± 0.878
6.629ValLeu: 6.629 ± 1.836
2.367ValMet: 2.367 ± 0.981
1.42ValAsn: 1.42 ± 1.02
5.208ValPro: 5.208 ± 1.255
1.42ValGln: 1.42 ± 0.525
2.841ValArg: 2.841 ± 1.183
4.735ValSer: 4.735 ± 1.142
7.576ValThr: 7.576 ± 2.459
4.261ValVal: 4.261 ± 1.441
0.0ValTrp: 0.0 ± 0.0
1.42ValTyr: 1.42 ± 0.736
0.0ValXaa: 0.0 ± 0.0
Trp
2.841TrpAla: 2.841 ± 1.493
0.473TrpCys: 0.473 ± 0.34
0.0TrpAsp: 0.0 ± 0.0
0.0TrpGlu: 0.0 ± 0.0
0.473TrpPhe: 0.473 ± 0.374
1.42TrpGly: 1.42 ± 0.928
0.473TrpHis: 0.473 ± 0.525
0.473TrpIle: 0.473 ± 0.418
0.947TrpLys: 0.947 ± 0.674
0.947TrpLeu: 0.947 ± 0.63
0.0TrpMet: 0.0 ± 0.0
0.473TrpAsn: 0.473 ± 0.34
1.42TrpPro: 1.42 ± 0.607
1.42TrpGln: 1.42 ± 1.118
0.473TrpArg: 0.473 ± 0.445
0.947TrpSer: 0.947 ± 0.483
1.42TrpThr: 1.42 ± 0.56
1.42TrpVal: 1.42 ± 0.769
0.473TrpTrp: 0.473 ± 0.34
0.947TrpTyr: 0.947 ± 0.596
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.894TyrAla: 1.894 ± 0.667
0.0TyrCys: 0.0 ± 0.0
0.947TyrAsp: 0.947 ± 0.516
2.841TyrGlu: 2.841 ± 0.987
1.42TyrPhe: 1.42 ± 0.736
1.42TyrGly: 1.42 ± 0.722
0.0TyrHis: 0.0 ± 0.0
0.473TyrIle: 0.473 ± 0.418
1.42TyrLys: 1.42 ± 0.637
1.42TyrLeu: 1.42 ± 0.928
0.473TyrMet: 0.473 ± 0.558
0.947TyrAsn: 0.947 ± 0.555
0.947TyrPro: 0.947 ± 0.556
1.42TyrGln: 1.42 ± 0.909
3.314TyrArg: 3.314 ± 1.163
0.473TyrSer: 0.473 ± 0.548
1.894TyrThr: 1.894 ± 0.91
2.367TyrVal: 2.367 ± 1.067
0.473TyrTrp: 0.473 ± 0.525
2.367TyrTyr: 2.367 ± 0.883
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 12 proteins (2113 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski