Candidatus Methanoplasma termitum
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1393 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7LA46|A0A0A7LA46_9ARCH Anthranilate synthase OS=Candidatus Methanoplasma termitum OX=1577791 GN=trpE PE=3 SV=1
MM1 pKa = 7.6 AGVKK5 pKa = 10.31 SKK7 pKa = 11.05 DD8 pKa = 3.06 ILKK11 pKa = 10.0 PKK13 pKa = 10.24 SKK15 pKa = 10.82 LIMTAIVALAVLAMVAAVYY34 pKa = 10.76 SPTSDD39 pKa = 3.34 AAARR43 pKa = 11.84 WYY45 pKa = 10.5 CVEE48 pKa = 4.52 NGSMTQSPASTTEE61 pKa = 3.72 YY62 pKa = 11.01 SSDD65 pKa = 3.29 QAAMTINANGWHH77 pKa = 5.79 YY78 pKa = 8.7 FIPSNTTATAPVTARR93 pKa = 11.84 NINVDD98 pKa = 4.13 SITVSGGSTANIILWSSSVWTFTLTVTNGITVANGSTLNIYY139 pKa = 7.23 VQPIDD144 pKa = 3.49 RR145 pKa = 11.84 TASTVRR151 pKa = 11.84 IGDD154 pKa = 3.57 AKK156 pKa = 10.63 IITPSIQLNGTAKK169 pKa = 10.38 LKK171 pKa = 10.72 NVGTIEE177 pKa = 3.96 VGNLFFSDD185 pKa = 3.72 NNNAAITANGTLNNYY200 pKa = 10.18 GIITNTSSGNGVTLTGDD217 pKa = 2.99 KK218 pKa = 11.16 GGIATVNNYY227 pKa = 10.83 GIFGTNVASTSISTLGGQIYY247 pKa = 10.57 ASTNGINVMASGGNITNDD265 pKa = 3.14 GVIQGGTYY273 pKa = 10.27 GINATPNVTVINNAAGTIQGNTTGINLAAGGTVTNSGLITGVSDD317 pKa = 3.54 IGIYY321 pKa = 10.14 TNGPNTQITNNNSPGGGVISGGTKK345 pKa = 10.24 GISVTAAAAGTTITNTGNIGGSQIGIDD372 pKa = 3.36 AQANVTVTNTDD383 pKa = 3.44 GEE385 pKa = 4.34 IHH387 pKa = 6.8 GDD389 pKa = 3.23 STAGINLAVGGTVTNTGVIASLNGNGIYY417 pKa = 10.68 SNGANTNITNNVGSTVPLGSIQGGNSGIYY446 pKa = 10.16 LNAGGDD452 pKa = 3.73 VNNYY456 pKa = 10.46 ALITGVSQGVYY467 pKa = 10.27 LPVGGNVNNQTSGLIYY483 pKa = 10.38 AAGGTMPASVGVSYY497 pKa = 10.76 GVNEE501 pKa = 4.42 TIVNYY506 pKa = 7.94 GTIMGDD512 pKa = 3.08 EE513 pKa = 3.98 NGIRR517 pKa = 11.84 FARR520 pKa = 11.84 GGTVTNYY527 pKa = 10.63 GSITGTIGYY536 pKa = 9.88 GIFSPMSIVEE546 pKa = 4.3 TMTVTNYY553 pKa = 8.49 NTITGKK559 pKa = 10.73 SIGIYY564 pKa = 9.97 LSAGGTVTNKK574 pKa = 10.17 PGGTITGTDD583 pKa = 2.93 NYY585 pKa = 10.78 GIVSDD590 pKa = 3.95 KK591 pKa = 10.98 DD592 pKa = 3.17 VWVINDD598 pKa = 3.53 GTIDD602 pKa = 3.74 GSSGIILTGGSVNKK616 pKa = 9.76 IDD618 pKa = 3.6 NTGDD622 pKa = 3.31 IIATGKK628 pKa = 10.41 QGISVINSTTNVEE641 pKa = 4.13 ITNSGQIKK649 pKa = 9.7 GASEE653 pKa = 4.61 GISINGDD660 pKa = 3.35 GIINNNSGTIIGGTAAISTAATSNSINILNDD691 pKa = 3.34 GLIEE695 pKa = 4.44 GNNSGIYY702 pKa = 10.68 LLGTQSTIEE711 pKa = 3.93 NRR713 pKa = 11.84 DD714 pKa = 3.69 TINGIGGIGISSAGGPTVLLNDD736 pKa = 3.65 GTITGDD742 pKa = 3.28 VTLLSGVVNNVTFITGSEE760 pKa = 3.57 IDD762 pKa = 3.73 GNFDD766 pKa = 3.37 IGDD769 pKa = 4.02 GNSTLDD775 pKa = 3.65 FPGAPDD781 pKa = 3.38 INLKK785 pKa = 10.54 YY786 pKa = 10.03 STVTGTTKK794 pKa = 10.1 IYY796 pKa = 10.95 LNTTVTFGNGWTSPPAIGLDD816 pKa = 3.78 DD817 pKa = 5.29 KK818 pKa = 11.32 IILIKK823 pKa = 10.01 GTTGGSMATYY833 pKa = 9.8 RR834 pKa = 11.84 YY835 pKa = 10.12 VSYY838 pKa = 9.81 TFNGEE843 pKa = 3.8 EE844 pKa = 4.05 FTILAANSKK853 pKa = 9.17 TDD855 pKa = 4.96 LIAKK859 pKa = 7.26 LTYY862 pKa = 8.81 VTADD866 pKa = 3.27 YY867 pKa = 11.6 VLIDD871 pKa = 4.16 GSQEE875 pKa = 3.66 QAPNQVFTLSQADD888 pKa = 4.08 FDD890 pKa = 4.55 NMGTFILDD898 pKa = 3.51 NTGSPNNGWYY908 pKa = 10.69 YY909 pKa = 8.81 ITEE912 pKa = 4.09 NVTLSNSITMSGDD925 pKa = 2.61 VSLIVRR931 pKa = 11.84 DD932 pKa = 3.75 SCTVTVTSGNGVEE945 pKa = 4.5 VPAGSSAAVYY955 pKa = 10.42 ANTRR959 pKa = 11.84 AGVDD963 pKa = 3.07 AGDD966 pKa = 3.59 IGVVTSTAAGAYY978 pKa = 8.62 GVNAAGNVINTASINIPSSTNGAVVSMGINASSQIDD1014 pKa = 3.92 IINGVTGIIQGRR1026 pKa = 11.84 GGINLTVGGMVDD1038 pKa = 2.84 NYY1040 pKa = 11.13 GIIKK1044 pKa = 10.24 ADD1046 pKa = 3.36 MGAGTGAGIQIGGNATVTNEE1066 pKa = 4.0 EE1067 pKa = 4.06 GGKK1070 pKa = 9.57 ILGSTGIYY1078 pKa = 9.57 CISGNVEE1085 pKa = 3.51 IDD1087 pKa = 3.13 NSGTIDD1093 pKa = 3.51 GTDD1096 pKa = 4.62 GIRR1099 pKa = 11.84 LITTSVCTITNNKK1112 pKa = 8.08 TGIIKK1117 pKa = 10.29 QSAGTGTSNAICYY1130 pKa = 8.32 SQSATGNIYY1139 pKa = 10.78 NYY1141 pKa = 10.55 GQILAEE1147 pKa = 4.37 KK1148 pKa = 9.47 GPAIFIDD1155 pKa = 3.61 TSYY1158 pKa = 10.69 ATTAVNTFNYY1168 pKa = 9.73 VNADD1172 pKa = 3.29 GTRR1175 pKa = 11.84 ALIKK1179 pKa = 10.4 GPYY1182 pKa = 8.83 AIQYY1186 pKa = 8.9 INSSRR1191 pKa = 11.84 GDD1193 pKa = 3.33 IYY1195 pKa = 11.55 NEE1197 pKa = 3.94 ADD1199 pKa = 3.6 VQSSTGYY1206 pKa = 10.59 AIQSSYY1212 pKa = 11.26 SGSKK1216 pKa = 7.86 ITNALGGKK1224 pKa = 8.19 LTGTGGISTSGVSVINAGDD1243 pKa = 4.08 IIANGTPGGNPSIGIISLGNVSIEE1267 pKa = 3.76 NSGNITAGLYY1277 pKa = 10.85 GITLSGGTVKK1287 pKa = 9.43 NTSAGNITVSGAYY1300 pKa = 8.71 NSNMIGIRR1308 pKa = 11.84 GTNGEE1313 pKa = 4.35 TIINDD1318 pKa = 3.0 GRR1320 pKa = 11.84 IDD1322 pKa = 3.57 VTTTGSVNAIGIRR1335 pKa = 11.84 LQAGGNITNNNVISGGNYY1353 pKa = 9.04 GVWSVGTVKK1362 pKa = 10.76 LSNYY1366 pKa = 10.26 DD1367 pKa = 3.84 SIIGNVTLDD1376 pKa = 3.35 NVANDD1381 pKa = 3.64 VVFGAGSSITGNFTMGTGGGTLKK1404 pKa = 9.96 FTDD1407 pKa = 4.15 PSGSLTTSALKK1418 pKa = 10.35 YY1419 pKa = 8.71 STVSGVANIGSALVSFDD1436 pKa = 4.67 GLPSDD1441 pKa = 3.6 YY1442 pKa = 11.0 DD1443 pKa = 3.32 LRR1445 pKa = 11.84 TIVLIDD1451 pKa = 3.67 ANTVVNTANAYY1462 pKa = 10.09 RR1463 pKa = 11.84 YY1464 pKa = 7.24 GTYY1467 pKa = 9.11 STVSPDD1473 pKa = 3.32 PVYY1476 pKa = 11.26 DD1477 pKa = 3.85 FVVLSQPEE1485 pKa = 3.87 RR1486 pKa = 11.84 LIAVIEE1492 pKa = 4.07 EE1493 pKa = 4.31 EE1494 pKa = 4.04 IPYY1497 pKa = 10.39 VDD1499 pKa = 4.19 VDD1501 pKa = 4.24 GNTQTHH1507 pKa = 4.5 TAIYY1511 pKa = 8.98 IDD1513 pKa = 3.79 NIFFEE1518 pKa = 4.67 RR1519 pKa = 11.84 SSGNGILYY1527 pKa = 7.72 DD1528 pKa = 4.01 TNTGGTDD1535 pKa = 3.48 GTWYY1539 pKa = 10.06 YY1540 pKa = 10.92 VPEE1543 pKa = 4.27 GTEE1546 pKa = 3.43 IDD1548 pKa = 3.87 LTGLAIRR1555 pKa = 11.84 DD1556 pKa = 3.66 LAASNSVSIIIGNDD1570 pKa = 3.38 GVLSLADD1577 pKa = 3.66 FVNVFTDD1584 pKa = 3.55 TFSIYY1589 pKa = 10.25 SQPEE1593 pKa = 3.71 GTGAIGILNNNINSRR1608 pKa = 11.84 INLPNGCEE1616 pKa = 4.19 FTNTAYY1622 pKa = 9.69 IYY1624 pKa = 8.98 RR1625 pKa = 11.84 QPIGSAQTEE1634 pKa = 4.4 SVIGNGVVTVTNGVTGVIKK1653 pKa = 10.57 GGQAISLTGGGSVYY1667 pKa = 10.7 NYY1669 pKa = 10.81 GDD1671 pKa = 4.18 IIGDD1675 pKa = 4.17 LYY1677 pKa = 11.36 DD1678 pKa = 3.75 GVYY1681 pKa = 10.05 VGYY1684 pKa = 10.82 GITNIINSQTSPGGGSGPGTISGKK1708 pKa = 10.23 NSGISIIANGNSSVINDD1725 pKa = 3.68 GTIVGLGTSGIAFMGYY1741 pKa = 8.94 SDD1743 pKa = 5.68 SIIEE1747 pKa = 4.05 ITNKK1751 pKa = 8.75 GTIEE1755 pKa = 4.27 GEE1757 pKa = 3.97 SSGINMNTGTVHH1769 pKa = 6.74 NSNTISGTGLGSTGIICNGTLTALGSEE1796 pKa = 4.59 IFNEE1800 pKa = 4.41 TGSSVISGVGAGVIMYY1816 pKa = 10.49 NGGTAVNNGSIIGTANGLFATAYY1839 pKa = 10.53 VDD1841 pKa = 3.52 LSNYY1845 pKa = 10.07 GSIKK1849 pKa = 10.7 GNVSLANVANDD1860 pKa = 3.09 VTFGVGSSIEE1870 pKa = 4.22 GNFNMGTAGGTLDD1883 pKa = 4.79 FVGQLVDD1890 pKa = 4.49 GVLTYY1895 pKa = 10.16 ATVTGASNIPQSTTVSFGNDD1915 pKa = 2.76 WDD1917 pKa = 4.27 NGTVTAPTLNAKK1929 pKa = 9.09 EE1930 pKa = 4.1 VIILIDD1936 pKa = 4.11 GSPSGTVSDD1945 pKa = 4.34 YY1946 pKa = 11.61 SSTYY1950 pKa = 8.8 TPNGSPIDD1958 pKa = 3.77 YY1959 pKa = 10.61 QIFTRR1964 pKa = 11.84 AYY1966 pKa = 8.11 PAGTTILDD1974 pKa = 3.45 QLIAYY1979 pKa = 7.11 PGTMIILIIDD1989 pKa = 3.85 GEE1991 pKa = 4.59 GSVQITGGTDD2001 pKa = 3.36 FDD2003 pKa = 3.8 EE2004 pKa = 5.18 TYY2006 pKa = 10.85 NGAGTYY2012 pKa = 10.02 QILVPFGIDD2021 pKa = 2.95 EE2022 pKa = 4.44 LTFTAIATVSGWNFVSFDD2040 pKa = 3.57 QNGAADD2046 pKa = 3.83 TFSPVTYY2053 pKa = 9.87 TIAQDD2058 pKa = 3.67 DD2059 pKa = 4.63 TVTATFDD2066 pKa = 3.56 EE2067 pKa = 4.7 TDD2069 pKa = 3.27 GTNDD2073 pKa = 3.19 VLNYY2077 pKa = 9.07 TLTIVGEE2084 pKa = 4.13 GDD2086 pKa = 3.94 VIVGTATFTGPGTWLMSVPIATPDD2110 pKa = 3.24 VTFEE2114 pKa = 3.91 ADD2116 pKa = 3.44 ANVTGWNFVSFDD2128 pKa = 3.57 QNGAADD2134 pKa = 3.83 TFSPVTYY2141 pKa = 9.64 TIADD2145 pKa = 3.94 GDD2147 pKa = 4.41 TVTATFDD2154 pKa = 3.56 EE2155 pKa = 4.7 TDD2157 pKa = 3.27 GTNDD2161 pKa = 3.19 VLNYY2165 pKa = 9.07 TLTIVGEE2172 pKa = 4.13 GDD2174 pKa = 3.94 VIVGTATFTGPGTWLMSVPIATPDD2198 pKa = 3.24 VTFEE2202 pKa = 3.91 ADD2204 pKa = 3.44 ANVTGWNFFSFDD2216 pKa = 3.8 QNGGTASRR2224 pKa = 11.84 YY2225 pKa = 10.01 SPMTYY2230 pKa = 9.15 TVAQGDD2236 pKa = 4.4 TITATFEE2243 pKa = 4.21 DD2244 pKa = 4.53 TTDD2247 pKa = 3.36 TTDD2250 pKa = 3.33 GMNTINYY2257 pKa = 7.35 TLTIVGKK2264 pKa = 10.8 GDD2266 pKa = 3.3 VDD2268 pKa = 3.6 NGTDD2272 pKa = 3.13 TFTGDD2277 pKa = 3.82 GSWLISMDD2285 pKa = 3.64 VVSPATTADD2294 pKa = 3.02 ITFTADD2300 pKa = 3.22 PNVAGWNFFSFDD2312 pKa = 3.8 QNGGTASRR2320 pKa = 11.84 YY2321 pKa = 10.01 SPMTYY2326 pKa = 9.32 TVVQNDD2332 pKa = 4.57 TITATFDD2339 pKa = 3.3 EE2340 pKa = 4.91 KK2341 pKa = 11.42 DD2342 pKa = 3.36 GVNDD2346 pKa = 3.52 VKK2348 pKa = 11.23 NITLTVTGNGSVAVDD2363 pKa = 3.82 GFPDD2367 pKa = 3.91 PFVGPGTWLLSVPDD2381 pKa = 3.95 TVTNLKK2387 pKa = 8.87 FTGTPTVPNGTVTFLQNGVGDD2408 pKa = 3.77 NTNPKK2413 pKa = 9.06 TFTIADD2419 pKa = 3.63 GDD2421 pKa = 4.07 IISAVFGSSGGPTEE2435 pKa = 3.99 PKK2437 pKa = 9.92 IYY2439 pKa = 10.04 TITATADD2446 pKa = 3.15 SGSTINPSGRR2456 pKa = 11.84 ITVQAGDD2463 pKa = 3.47 SKK2465 pKa = 10.17 TFTFAAKK2472 pKa = 10.15 DD2473 pKa = 3.54 GYY2475 pKa = 9.53 TIVEE2479 pKa = 3.84 VWIDD2483 pKa = 3.44 GVYY2486 pKa = 10.63 SLSQAEE2492 pKa = 4.41 IDD2494 pKa = 3.61 SGSYY2498 pKa = 9.15 TFSNVMSNHH2507 pKa = 6.55 TIQMKK2512 pKa = 10.12 SVVGSRR2518 pKa = 11.84 TSDD2521 pKa = 2.6 ITLTINIKK2529 pKa = 10.62 EE2530 pKa = 4.03 GDD2532 pKa = 3.67 GYY2534 pKa = 11.61 AEE2536 pKa = 3.88 YY2537 pKa = 10.38 SINGGSFTRR2546 pKa = 11.84 YY2547 pKa = 7.42 TGPVTLQVGDD2557 pKa = 3.94 NVAVRR2562 pKa = 11.84 AFAADD2567 pKa = 4.88 GYY2569 pKa = 11.29 QFAKK2573 pKa = 10.18 WEE2575 pKa = 4.33 TPSVEE2580 pKa = 4.52 TNSSLSFNDD2589 pKa = 3.05 VRR2591 pKa = 11.84 ASMHH2595 pKa = 6.65 LDD2597 pKa = 3.57 LYY2599 pKa = 9.77 FTVAVTGAVDD2609 pKa = 3.65 NKK2611 pKa = 10.59 DD2612 pKa = 4.22 GSALWIIGLLILVILLALFLLFLLWIRR2639 pKa = 11.84 AGLFLSITMGGEE2651 pKa = 4.03 AVNGAAVTYY2660 pKa = 10.07 VVVNDD2665 pKa = 4.7 DD2666 pKa = 4.12 GKK2668 pKa = 9.35 TKK2670 pKa = 10.85 NGVKK2674 pKa = 10.19 SSSSRR2679 pKa = 11.84 GKK2681 pKa = 10.56 LRR2683 pKa = 11.84 IPAKK2687 pKa = 10.46 KK2688 pKa = 9.84 NATVTITMAAKK2699 pKa = 10.17 DD2700 pKa = 3.57 GHH2702 pKa = 6.54 IATGLPLIVSMEE2714 pKa = 3.57 NRR2716 pKa = 11.84 RR2717 pKa = 11.84 EE2718 pKa = 3.91 HH2719 pKa = 8.15 RR2720 pKa = 11.84 EE2721 pKa = 4.05 MILKK2725 pKa = 10.18
Molecular weight: 279.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.935
IPC_protein 3.986
Toseland 3.757
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.126
Wikipedia 3.923
Rodwell 3.808
Grimsley 3.656
Solomon 3.973
Lehninger 3.935
Nozaki 4.088
DTASelect 4.368
Thurlkill 3.795
EMBOSS 3.923
Sillero 4.101
Patrickios 1.062
IPC_peptide 3.973
IPC2_peptide 4.088
IPC2.peptide.svr19 3.989
Protein with the highest isoelectric point:
>tr|A0A0A7LDI5|A0A0A7LDI5_9ARCH Phosphoribosylamine--glycine ligase OS=Candidatus Methanoplasma termitum OX=1577791 GN=purD PE=3 SV=1
MM1 pKa = 7.7 SSTKK5 pKa = 9.43 PAAMKK10 pKa = 10.59 GRR12 pKa = 11.84 LNKK15 pKa = 10.04 KK16 pKa = 8.23 IKK18 pKa = 9.07 QNRR21 pKa = 11.84 RR22 pKa = 11.84 VPAWVMMRR30 pKa = 11.84 TNRR33 pKa = 11.84 QFLRR37 pKa = 11.84 HH38 pKa = 5.85 PKK40 pKa = 8.59 RR41 pKa = 11.84 RR42 pKa = 11.84 SWRR45 pKa = 11.84 MSKK48 pKa = 10.58 LKK50 pKa = 10.36 EE51 pKa = 3.79
Molecular weight: 6.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.382
IPC2_protein 10.833
IPC_protein 12.384
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.369
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.036
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1393
0
1393
445219
29
3233
319.6
35.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.774 ± 0.085
1.377 ± 0.035
6.061 ± 0.057
6.535 ± 0.121
3.862 ± 0.052
8.189 ± 0.144
1.549 ± 0.031
7.751 ± 0.06
6.778 ± 0.116
7.878 ± 0.084
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.295 ± 0.051
4.227 ± 0.077
3.834 ± 0.04
2.118 ± 0.031
4.543 ± 0.093
6.668 ± 0.072
5.888 ± 0.164
7.446 ± 0.057
0.902 ± 0.025
3.324 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here