Lysobacter antibioticus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5139 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2FBQ0|A0A0S2FBQ0_9GAMM Glutathione S-transferase N-terminal domain protein OS=Lysobacter antibioticus OX=84531 GN=LA76x_2845 PE=4 SV=1
MM1 pKa = 7.34WQFQTIRR8 pKa = 11.84NPSEE12 pKa = 3.61ALLWFSDD19 pKa = 3.85GLPIDD24 pKa = 3.63SWRR27 pKa = 11.84GGQGVSSRR35 pKa = 11.84LIVDD39 pKa = 3.7GEE41 pKa = 4.51GFLDD45 pKa = 3.72VTDD48 pKa = 4.5FQNGFPFGPVMDD60 pKa = 5.93FIDD63 pKa = 4.24FNGADD68 pKa = 4.16FDD70 pKa = 4.3YY71 pKa = 10.53TPNPSASFEE80 pKa = 4.42LYY82 pKa = 10.87LPGDD86 pKa = 3.83GSFTATAAATGVSRR100 pKa = 11.84SGQLKK105 pKa = 9.16PLPVVAAADD114 pKa = 3.49AAYY117 pKa = 9.96LDD119 pKa = 4.7RR120 pKa = 11.84MIADD124 pKa = 4.16KK125 pKa = 10.83YY126 pKa = 11.29VPYY129 pKa = 10.48QDD131 pKa = 5.22IPDD134 pKa = 3.99APGKK138 pKa = 10.59SFAQIAALGAQLFPFSPYY156 pKa = 10.58SFQLAMSIYY165 pKa = 10.19DD166 pKa = 3.26WTTASFTRR174 pKa = 11.84LVFMKK179 pKa = 9.92IFEE182 pKa = 4.34YY183 pKa = 10.38TGMSQSPLPLDD194 pKa = 3.5QDD196 pKa = 4.64SIATAIWEE204 pKa = 4.9SNWNTYY210 pKa = 10.36NPGNADD216 pKa = 3.54YY217 pKa = 9.89MNSFMMTPASSLADD231 pKa = 3.52VQSQLAAVATQLQQFSDD248 pKa = 3.77VEE250 pKa = 4.13NRR252 pKa = 11.84LLAAAYY258 pKa = 10.15QSMPRR263 pKa = 11.84TSIVDD268 pKa = 3.54QPQLFSGQMDD278 pKa = 4.09IYY280 pKa = 10.22QLGMEE285 pKa = 4.36HH286 pKa = 6.95FGIEE290 pKa = 4.57FLQCPLNAGPDD301 pKa = 3.87TVPLQIDD308 pKa = 4.28FNQAIADD315 pKa = 3.99YY316 pKa = 9.79IRR318 pKa = 11.84PGDD321 pKa = 3.89TITTKK326 pKa = 10.09MVWSFGSSMSEE337 pKa = 3.22AMQYY341 pKa = 10.67QNGIVLVANPPDD353 pKa = 4.34GAWVWDD359 pKa = 3.65AASYY363 pKa = 7.79ITPLSDD369 pKa = 4.5DD370 pKa = 3.94PDD372 pKa = 5.61KK373 pKa = 11.27IEE375 pKa = 3.83YY376 pKa = 9.11TFAPGTSFLVQSVEE390 pKa = 3.85QTQNNGVDD398 pKa = 3.23VWVITLLVSGAA409 pKa = 3.6

Molecular weight: 44.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2F4U7|A0A0S2F4U7_9GAMM Uncharacterized protein OS=Lysobacter antibioticus OX=84531 GN=LA76x_0397 PE=4 SV=1
MM1 pKa = 7.08TGKK4 pKa = 9.97AAKK7 pKa = 9.4QIKK10 pKa = 9.87ISATATATATATATATATATAMIRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84GGSWVGAAQAATATSQLRR54 pKa = 11.84RR55 pKa = 11.84NHH57 pKa = 6.09RR58 pKa = 11.84FAVAARR64 pKa = 11.84AAPTPKK70 pKa = 9.61QRR72 pKa = 11.84PPSQRR77 pKa = 11.84KK78 pKa = 8.8RR79 pKa = 11.84GALRR83 pKa = 11.84PVSVPRR89 pKa = 4.12

Molecular weight: 9.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5139

0

5139

1660852

37

9268

323.2

35.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.153 ± 0.053

0.852 ± 0.013

5.903 ± 0.034

5.272 ± 0.04

3.324 ± 0.022

8.719 ± 0.068

2.125 ± 0.022

4.116 ± 0.025

2.763 ± 0.032

10.416 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.947 ± 0.02

2.731 ± 0.04

5.348 ± 0.031

3.68 ± 0.027

7.73 ± 0.057

5.767 ± 0.035

5.043 ± 0.071

7.073 ± 0.031

1.543 ± 0.02

2.495 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski