Saccharothrix syringae (Nocardiopsis syringae)

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8856 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q0GZ91|A0A5Q0GZ91_SACSY GlsB/YeaQ/YmgE family stress response membrane protein OS=Saccharothrix syringae OX=103733 GN=EKG83_16605 PE=3 SV=1
MM1 pKa = 7.58PRR3 pKa = 11.84RR4 pKa = 11.84HH5 pKa = 6.08RR6 pKa = 11.84NEE8 pKa = 3.89VALSTTPTSRR18 pKa = 11.84PRR20 pKa = 11.84TAVVRR25 pKa = 11.84RR26 pKa = 11.84LRR28 pKa = 11.84VLGSATTLALASLVAAGAPAHH49 pKa = 6.41AAFPGDD55 pKa = 3.06NGKK58 pKa = 9.81IYY60 pKa = 9.13FTEE63 pKa = 4.11SGNLYY68 pKa = 8.96TVNPDD73 pKa = 2.92GSGVTQVLGSPGANAPRR90 pKa = 11.84VNAQGTKK97 pKa = 9.02VAFEE101 pKa = 4.54RR102 pKa = 11.84GVNSPDD108 pKa = 3.56LQVHH112 pKa = 5.2VMNPDD117 pKa = 2.96GTGITAITSGAGADD131 pKa = 3.99GQSLAWSPDD140 pKa = 2.96GTKK143 pKa = 10.58LIYY146 pKa = 10.38RR147 pKa = 11.84PGGTLTVINADD158 pKa = 3.5GSNPTPLGVSGNHH171 pKa = 6.45PNWSPDD177 pKa = 2.65GSKK180 pKa = 9.9IVYY183 pKa = 9.43QDD185 pKa = 2.96SGVIKK190 pKa = 9.45TVNPDD195 pKa = 2.87GTGQTTLASTPGVTYY210 pKa = 9.02YY211 pKa = 11.17APNWSPDD218 pKa = 2.96SSKK221 pKa = 9.44ITFTAFSFDD230 pKa = 3.46PLGNHH235 pKa = 6.14VYY237 pKa = 10.33TMNADD242 pKa = 3.64GTNQTSLADD251 pKa = 4.24GYY253 pKa = 11.39NSVWSPDD260 pKa = 3.13GTKK263 pKa = 9.66IQYY266 pKa = 9.72QDD268 pKa = 3.42NNGLLLMNPDD278 pKa = 3.23GTNQTRR284 pKa = 11.84LYY286 pKa = 9.09TFTFGIVTTGDD297 pKa = 2.95WAAIPPVGADD307 pKa = 3.16VSVSVTDD314 pKa = 3.47SADD317 pKa = 3.59PVSLGGDD324 pKa = 3.56PYY326 pKa = 11.33SYY328 pKa = 10.08TVSVANAGPAGATGVAVSTTLSGAAASIDD357 pKa = 3.77SATSSQGSCSVSAPTVSCTLGGVAASGSATVTITVTPSATGTVTATSTVSATGTDD412 pKa = 3.63PTPGNNTDD420 pKa = 3.82AEE422 pKa = 4.57STTVNNALGCTITGTNGPDD441 pKa = 3.16TLTGTNGNDD450 pKa = 3.69VICGLGGNDD459 pKa = 4.23TISGGNGADD468 pKa = 3.62TIYY471 pKa = 11.08AGAGNDD477 pKa = 4.67AINGGNGNDD486 pKa = 3.41TVYY489 pKa = 11.06AGAGDD494 pKa = 3.84DD495 pKa = 3.56SSYY498 pKa = 11.9GEE500 pKa = 4.81DD501 pKa = 4.9LLGLLDD507 pKa = 4.41NGADD511 pKa = 3.56TLYY514 pKa = 10.86GGPGNDD520 pKa = 3.98TLDD523 pKa = 3.88GQNGNDD529 pKa = 3.44ILYY532 pKa = 10.46DD533 pKa = 3.55HH534 pKa = 7.88DD535 pKa = 4.24GTDD538 pKa = 3.51TLSGGVGNDD547 pKa = 3.8SIDD550 pKa = 3.52VQDD553 pKa = 4.18GVGGDD558 pKa = 3.68TANGGLGTDD567 pKa = 3.33TCTVDD572 pKa = 3.83GGDD575 pKa = 3.42ITTSCC580 pKa = 4.24

Molecular weight: 57.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q0HDM0|A0A5Q0HDM0_SACSY Uncharacterized protein OS=Saccharothrix syringae OX=103733 GN=EKG83_06205 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 8.7THH19 pKa = 5.15GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.77GRR42 pKa = 11.84KK43 pKa = 7.91QLSAA47 pKa = 3.9

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8856

0

8856

3125302

24

11053

352.9

37.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.884 ± 0.044

0.783 ± 0.008

6.259 ± 0.021

5.419 ± 0.023

2.754 ± 0.015

9.527 ± 0.027

2.441 ± 0.014

2.394 ± 0.015

1.495 ± 0.016

10.608 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.386 ± 0.009

1.747 ± 0.017

6.254 ± 0.025

2.412 ± 0.015

8.848 ± 0.027

4.549 ± 0.02

5.894 ± 0.029

9.882 ± 0.028

1.585 ± 0.012

1.88 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski