Pseudomonas phage vB_PaeP_C2-10_Ab09

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Migulavirinae; Litunavirus; Pseudomonas virus Ab09

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X5L0X1|X5L0X1_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_C2-10_Ab09 OX=1476391 GN=ORF07 PE=4 SV=1
MM1 pKa = 7.68DD2 pKa = 3.85QNIYY6 pKa = 8.78TLLYY10 pKa = 10.35TNEE13 pKa = 4.22CLHH16 pKa = 7.1SIEE19 pKa = 4.55QEE21 pKa = 4.03FEE23 pKa = 4.03AVSDD27 pKa = 3.68EE28 pKa = 4.19VARR31 pKa = 11.84IKK33 pKa = 9.61ATAMCRR39 pKa = 11.84GQEE42 pKa = 4.22GLWSGIYY49 pKa = 9.14LTDD52 pKa = 3.9PQGEE56 pKa = 4.89AITFDD61 pKa = 3.84PFF63 pKa = 4.15

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X5KIJ3|X5KIJ3_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_C2-10_Ab09 OX=1476391 GN=ORF09 PE=4 SV=1
MM1 pKa = 6.64NTLYY5 pKa = 10.85HH6 pKa = 7.09KK7 pKa = 9.67MQEE10 pKa = 4.32HH11 pKa = 5.76VCFVGFTRR19 pKa = 11.84AGMDD23 pKa = 3.24HH24 pKa = 7.16LSNRR28 pKa = 11.84VHH30 pKa = 6.2TFGEE34 pKa = 4.07ARR36 pKa = 11.84HH37 pKa = 6.56FITFNCRR44 pKa = 11.84PVTRR48 pKa = 11.84TSASSTAFPMKK59 pKa = 9.69GQRR62 pKa = 11.84MWKK65 pKa = 9.96

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

22799

61

3398

274.7

30.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.031 ± 0.57

0.952 ± 0.178

6.035 ± 0.166

6.816 ± 0.3

3.671 ± 0.177

6.82 ± 0.265

1.904 ± 0.224

4.869 ± 0.223

5.812 ± 0.28

8.878 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.215 ± 0.173

4.483 ± 0.156

4.211 ± 0.214

4.461 ± 0.261

5.562 ± 0.194

5.768 ± 0.221

5.996 ± 0.258

7.123 ± 0.224

1.281 ± 0.147

3.114 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski