Acinetobacter sp. CIP 102637
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2615 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|N8XIL4|N8XIL4_9GAMM Uncharacterized protein OS=Acinetobacter sp. CIP 102637 OX=1144669 GN=F967_00589 PE=3 SV=1
MM1 pKa = 7.69 SYY3 pKa = 10.62 YY4 pKa = 10.57 HH5 pKa = 7.6 ILIEE9 pKa = 4.38 VNDD12 pKa = 4.95 HH13 pKa = 6.66 ISTIEE18 pKa = 3.59 QTRR21 pKa = 11.84 DD22 pKa = 3.15 VEE24 pKa = 4.37 ILDD27 pKa = 3.81 IQDD30 pKa = 3.23 ISPYY34 pKa = 9.58 LHH36 pKa = 7.12 SILIPYY42 pKa = 9.93 FNEE45 pKa = 3.84 QEE47 pKa = 4.16 IEE49 pKa = 4.23 LEE51 pKa = 4.34 DD52 pKa = 3.7 EE53 pKa = 3.95 NVAYY57 pKa = 10.31 DD58 pKa = 4.39 EE59 pKa = 4.32 ILHH62 pKa = 6.67 LEE64 pKa = 4.45 VKK66 pKa = 8.86 QTLLPIEE73 pKa = 4.24 HH74 pKa = 7.25 LIEE77 pKa = 4.48 EE78 pKa = 4.45 EE79 pKa = 4.15 QKK81 pKa = 10.59 QLPSDD86 pKa = 3.56 TDD88 pKa = 3.61 VTITAYY94 pKa = 10.19 EE95 pKa = 3.98 IFNDD99 pKa = 3.69 RR100 pKa = 11.84 DD101 pKa = 3.11 LSQDD105 pKa = 3.04 VTAVIFDD112 pKa = 3.38 ILEE115 pKa = 4.1 AVKK118 pKa = 10.82 LDD120 pKa = 4.0 DD121 pKa = 5.12 APP123 pKa = 4.9
Molecular weight: 14.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.872
IPC_protein 3.834
Toseland 3.643
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.668
Grimsley 3.554
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.101
Thurlkill 3.681
EMBOSS 3.732
Sillero 3.948
Patrickios 1.875
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.854
Protein with the highest isoelectric point:
>tr|N8XDV9|N8XDV9_9GAMM FA_desaturase domain-containing protein OS=Acinetobacter sp. CIP 102637 OX=1144669 GN=F967_02350 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.97 LKK11 pKa = 10.13 RR12 pKa = 11.84 KK13 pKa = 8.98 RR14 pKa = 11.84 VHH16 pKa = 6.36 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 AGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.18 GRR39 pKa = 11.84 HH40 pKa = 5.1 SLTVV44 pKa = 3.06
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2615
0
2615
782047
20
3312
299.1
33.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.676 ± 0.058
0.959 ± 0.018
5.27 ± 0.04
5.941 ± 0.049
4.163 ± 0.035
6.379 ± 0.051
2.433 ± 0.03
6.726 ± 0.041
5.646 ± 0.047
10.486 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.494 ± 0.025
4.358 ± 0.043
3.891 ± 0.03
5.803 ± 0.058
4.524 ± 0.036
5.893 ± 0.042
5.327 ± 0.049
6.604 ± 0.041
1.238 ± 0.017
3.19 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here