Chitinophaga sancti

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6697 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1K1SXU0|A0A1K1SXU0_9BACT Arabinose-5-phosphate isomerase OS=Chitinophaga sancti OX=1004 GN=SAMN05661012_06366 PE=3 SV=1
VV1 pKa = 6.14TVVEE5 pKa = 4.28DD6 pKa = 4.06TPTSFEE12 pKa = 3.9VRR14 pKa = 11.84TNDD17 pKa = 3.07TDD19 pKa = 3.93VEE21 pKa = 4.42GDD23 pKa = 3.49ALTATVIAGPVNGTVVPNPDD43 pKa = 3.11GSFKK47 pKa = 10.09YY48 pKa = 9.2TPNANYY54 pKa = 10.58NGLDD58 pKa = 3.31SLKK61 pKa = 10.97YY62 pKa = 8.46QVCDD66 pKa = 2.72NGTPSKK72 pKa = 10.67CDD74 pKa = 2.84TGTVIFTVTPVNDD87 pKa = 3.48APVAVDD93 pKa = 3.63DD94 pKa = 4.61AVTVTEE100 pKa = 4.38DD101 pKa = 3.31TPTDD105 pKa = 3.74FEE107 pKa = 4.73VRR109 pKa = 11.84TNDD112 pKa = 3.09TDD114 pKa = 3.93VEE116 pKa = 4.42GDD118 pKa = 3.49ALTATVITGPVHH130 pKa = 5.43GTVSQKK136 pKa = 10.54PDD138 pKa = 2.65GSYY141 pKa = 10.23TYY143 pKa = 10.58TPALNYY149 pKa = 10.61NGLDD153 pKa = 3.31SLKK156 pKa = 10.91YY157 pKa = 8.48QVCDD161 pKa = 2.56SGTPSKK167 pKa = 10.87CDD169 pKa = 2.85TGTVIFTVTPVNDD182 pKa = 3.59SPVAVDD188 pKa = 3.71DD189 pKa = 4.19AVTVVEE195 pKa = 4.39DD196 pKa = 4.06TPTSFEE202 pKa = 3.9VRR204 pKa = 11.84TNDD207 pKa = 3.07TDD209 pKa = 3.93VEE211 pKa = 4.42GDD213 pKa = 3.49ALTATVIAGPVNGTVVPNPDD233 pKa = 2.38GSFRR237 pKa = 11.84YY238 pKa = 7.81TPNANYY244 pKa = 10.6NGLDD248 pKa = 3.31SLKK251 pKa = 10.97YY252 pKa = 8.46QVCDD256 pKa = 2.72NGTPSKK262 pKa = 10.67CDD264 pKa = 2.87TGTVIFKK271 pKa = 7.52VTPVNDD277 pKa = 3.43APVAVDD283 pKa = 3.52DD284 pKa = 4.45AVTVVEE290 pKa = 4.59DD291 pKa = 4.03TPTDD295 pKa = 3.66FDD297 pKa = 4.15VRR299 pKa = 11.84TNDD302 pKa = 2.95TDD304 pKa = 4.02AEE306 pKa = 4.38GDD308 pKa = 3.6ALTANVISGPVHH320 pKa = 6.95GIISQNPDD328 pKa = 2.29GSFKK332 pKa = 10.07YY333 pKa = 9.2TPNANYY339 pKa = 10.58NGLDD343 pKa = 3.33SLKK346 pKa = 10.72YY347 pKa = 9.48RR348 pKa = 11.84VCDD351 pKa = 3.19NGTPSMCNTGTVIFTVTPVNDD372 pKa = 3.48APVAVDD378 pKa = 3.52DD379 pKa = 4.45AVTVVEE385 pKa = 4.59DD386 pKa = 4.03TPTDD390 pKa = 3.69FDD392 pKa = 3.93VRR394 pKa = 11.84ANDD397 pKa = 3.54TDD399 pKa = 3.95VEE401 pKa = 4.44GDD403 pKa = 3.49ALTATVITGPVNGTVSPNPDD423 pKa = 2.71GSFKK427 pKa = 10.09YY428 pKa = 9.2TPNANYY434 pKa = 10.58NGLDD438 pKa = 3.31SLKK441 pKa = 10.97YY442 pKa = 8.46QVCDD446 pKa = 2.93NGTPSMCNTGTVIFTVTPVNDD467 pKa = 3.38APIAVDD473 pKa = 3.6DD474 pKa = 4.44AVTVTEE480 pKa = 4.48DD481 pKa = 3.27TPTDD485 pKa = 3.59FDD487 pKa = 4.15VRR489 pKa = 11.84TNDD492 pKa = 3.13TDD494 pKa = 3.89VEE496 pKa = 4.42GDD498 pKa = 3.49ALTATVITGPVNGTVSQNPDD518 pKa = 2.51GSFKK522 pKa = 10.07YY523 pKa = 9.2TPNANYY529 pKa = 10.58NGLDD533 pKa = 3.31SLKK536 pKa = 10.97YY537 pKa = 8.46QVCDD541 pKa = 2.72NGTPSKK547 pKa = 10.67CDD549 pKa = 2.84TGTVIFTVTPVNDD562 pKa = 3.38APIAVDD568 pKa = 3.42DD569 pKa = 4.31AVPVVEE575 pKa = 4.64DD576 pKa = 4.03TPTSFEE582 pKa = 3.9VRR584 pKa = 11.84TNDD587 pKa = 2.87TDD589 pKa = 4.12AEE591 pKa = 4.4GDD593 pKa = 3.63ALTATVITGPVNGTVSQNPDD613 pKa = 2.51GSFKK617 pKa = 10.07YY618 pKa = 9.2TPNANYY624 pKa = 10.58NGLDD628 pKa = 3.36SLKK631 pKa = 10.65YY632 pKa = 9.42RR633 pKa = 11.84VYY635 pKa = 11.52DD636 pKa = 3.25NGTSSKK642 pKa = 10.39CDD644 pKa = 2.91TGTVIFTVTPVNDD657 pKa = 3.38APIAVDD663 pKa = 3.6DD664 pKa = 4.44AVTVTEE670 pKa = 4.26DD671 pKa = 3.38TPTSFEE677 pKa = 3.9VRR679 pKa = 11.84TNDD682 pKa = 3.07TDD684 pKa = 3.93VEE686 pKa = 4.42GDD688 pKa = 3.49ALTATVITGPVHH700 pKa = 5.73GTVSPNPDD708 pKa = 2.71GSFKK712 pKa = 10.09YY713 pKa = 9.2TPNANYY719 pKa = 10.58NGLDD723 pKa = 3.31SLKK726 pKa = 10.97YY727 pKa = 8.46QVCDD731 pKa = 2.93NGTPSMCNTGTVIFTVTPVNDD752 pKa = 3.38APIAVDD758 pKa = 3.6DD759 pKa = 4.44AVTVTEE765 pKa = 5.18DD766 pKa = 3.26APTDD770 pKa = 4.01FNVLANDD777 pKa = 3.67TDD779 pKa = 4.12VEE781 pKa = 4.42GDD783 pKa = 3.49ALTATVIISPLHH795 pKa = 4.57GTITLKK801 pKa = 11.23GNGLYY806 pKa = 9.99TYY808 pKa = 9.97TPAANYY814 pKa = 10.2NGLDD818 pKa = 3.35SLKK821 pKa = 10.91YY822 pKa = 8.48QVCDD826 pKa = 2.62SGTPGKK832 pKa = 10.33CDD834 pKa = 2.87TGTVIFTVTPVNDD847 pKa = 3.67APAGTDD853 pKa = 3.21QTITIPKK860 pKa = 7.34NTTYY864 pKa = 10.49TGTIKK869 pKa = 10.84GSDD872 pKa = 3.36ADD874 pKa = 4.28GDD876 pKa = 4.01VLTYY880 pKa = 10.69SQASSAAHH888 pKa = 6.29GNASVNPDD896 pKa = 2.52GSFTYY901 pKa = 10.2KK902 pKa = 10.7PNTDD906 pKa = 3.33YY907 pKa = 11.53VGNDD911 pKa = 3.01SFTITISDD919 pKa = 3.57GHH921 pKa = 7.23LSTTVTINITVTQVVAHH938 pKa = 6.45PGITLVKK945 pKa = 8.87TAEE948 pKa = 4.15LKK950 pKa = 11.28GNVITYY956 pKa = 7.54TFTTTNTGDD965 pKa = 3.28VALTNVILADD975 pKa = 3.54PMVNLNTTISTTLQPGEE992 pKa = 4.54VVTQTTTYY1000 pKa = 10.48TITQTDD1006 pKa = 3.91RR1007 pKa = 11.84EE1008 pKa = 4.29NGSVSNTASVSGTSSDD1024 pKa = 3.35HH1025 pKa = 5.86KK1026 pKa = 9.65TVADD1030 pKa = 3.8ISGTSANNDD1039 pKa = 2.91IPTTITVPGVIKK1051 pKa = 10.96ANDD1054 pKa = 3.62DD1055 pKa = 3.8EE1056 pKa = 5.8YY1057 pKa = 10.68DD1058 pKa = 3.35TRR1060 pKa = 11.84MNTHH1064 pKa = 6.58INFNPLNNDD1073 pKa = 3.08NLGTSTLQTINTVTTVQHH1091 pKa = 5.0GTITFDD1097 pKa = 3.26VNGNFEE1103 pKa = 4.21YY1104 pKa = 10.74TPDD1107 pKa = 3.1QGYY1110 pKa = 10.4IGDD1113 pKa = 3.89DD1114 pKa = 3.25TFTYY1118 pKa = 10.07TITDD1122 pKa = 3.24ADD1124 pKa = 4.33GYY1126 pKa = 9.74VSNIATVTIHH1136 pKa = 6.71IGAVYY1141 pKa = 10.13IKK1143 pKa = 10.43VPTLFTPNGDD1153 pKa = 3.39GKK1155 pKa = 10.63NDD1157 pKa = 3.17VFEE1160 pKa = 4.45IVGLDD1165 pKa = 3.19KK1166 pKa = 11.16YY1167 pKa = 9.46TDD1169 pKa = 3.44NEE1171 pKa = 4.45LIIVNRR1177 pKa = 11.84WGNEE1181 pKa = 3.46VYY1183 pKa = 10.22RR1184 pKa = 11.84QHH1186 pKa = 6.87NYY1188 pKa = 9.89SNTWNGDD1195 pKa = 3.27GLSEE1199 pKa = 3.86GTYY1202 pKa = 10.6YY1203 pKa = 11.33YY1204 pKa = 11.18LLRR1207 pKa = 11.84VKK1209 pKa = 10.47KK1210 pKa = 9.92QDD1212 pKa = 3.37SAEE1215 pKa = 3.93WEE1217 pKa = 4.32VFKK1220 pKa = 11.3GFTTILRR1227 pKa = 11.84KK1228 pKa = 9.75LNKK1231 pKa = 9.92

Molecular weight: 129.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1K1R6W4|A0A1K1R6W4_9BACT CubicO group peptidase beta-lactamase class C family OS=Chitinophaga sancti OX=1004 GN=SAMN05661012_03425 PE=4 SV=1
MM1 pKa = 7.26LRR3 pKa = 11.84WTVTFLIIALVAALFGFGGIAASAAGIAKK32 pKa = 9.97IIFFIFLVLLVISLVMGRR50 pKa = 11.84RR51 pKa = 11.84SRR53 pKa = 11.84II54 pKa = 3.41

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6697

0

6697

2424268

39

7223

362.0

40.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.914 ± 0.034

0.858 ± 0.011

5.285 ± 0.02

5.363 ± 0.031

4.583 ± 0.02

6.956 ± 0.029

2.043 ± 0.015

6.776 ± 0.028

6.229 ± 0.029

9.459 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.447 ± 0.014

5.344 ± 0.029

4.046 ± 0.02

3.955 ± 0.018

4.179 ± 0.023

6.253 ± 0.028

6.16 ± 0.04

6.491 ± 0.024

1.283 ± 0.011

4.377 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski