Brevundimonas viscosa

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Brevundimonas

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3072 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I6NRL4|A0A1I6NRL4_9CAUL Dihydrolipoyl dehydrogenase OS=Brevundimonas viscosa OX=871741 GN=SAMN05192570_0398 PE=3 SV=1
MM1 pKa = 7.55KK2 pKa = 10.28KK3 pKa = 10.52LLIAALAATLASATGAAAQDD23 pKa = 3.59ASATATYY30 pKa = 9.75GQTRR34 pKa = 11.84LRR36 pKa = 11.84SGFTPDD42 pKa = 3.1PHH44 pKa = 7.36SVRR47 pKa = 11.84ITAGGSIDD55 pKa = 3.35ASRR58 pKa = 11.84IGSPCTGSIANAPDD72 pKa = 3.39YY73 pKa = 10.84EE74 pKa = 4.45VTYY77 pKa = 9.39TAGSLPLYY85 pKa = 10.05IYY87 pKa = 10.65AQADD91 pKa = 3.41SDD93 pKa = 3.92TTLVVNGPDD102 pKa = 3.92GQWYY106 pKa = 10.43CDD108 pKa = 3.77DD109 pKa = 5.02DD110 pKa = 5.67SNGGLNPQVTFNSPRR125 pKa = 11.84SGTYY129 pKa = 9.87DD130 pKa = 2.47IWVGTFGGGTASATLHH146 pKa = 5.99ISEE149 pKa = 5.59LSDD152 pKa = 4.44DD153 pKa = 4.92DD154 pKa = 5.3SGPDD158 pKa = 3.83PDD160 pKa = 5.66DD161 pKa = 4.29SSAMPDD167 pKa = 2.89ASLRR171 pKa = 11.84ATYY174 pKa = 11.02GEE176 pKa = 3.95VSLRR180 pKa = 11.84NGFTPDD186 pKa = 3.14PHH188 pKa = 6.85RR189 pKa = 11.84VSLTAGGSVPASNVSSSCSGSIAVAPDD216 pKa = 3.27YY217 pKa = 10.94QVTYY221 pKa = 9.27TAGSLPLVFRR231 pKa = 11.84SQSSTDD237 pKa = 3.07TTLVINGPDD246 pKa = 4.13GQWSCDD252 pKa = 3.55DD253 pKa = 6.12DD254 pKa = 4.28GWQSNNAEE262 pKa = 3.97IRR264 pKa = 11.84FDD266 pKa = 3.52KK267 pKa = 10.73PMSGVYY273 pKa = 9.76DD274 pKa = 3.28VWVGVYY280 pKa = 10.59GGGTARR286 pKa = 11.84ADD288 pKa = 3.69LLVTEE293 pKa = 4.98VPP295 pKa = 3.84

Molecular weight: 30.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6SML5|A0A1I6SML5_9CAUL Uncharacterized protein OS=Brevundimonas viscosa OX=871741 GN=SAMN05192570_2587 PE=4 SV=1
MM1 pKa = 8.09RR2 pKa = 11.84LPPIATVLGGLLLSRR17 pKa = 11.84GGRR20 pKa = 11.84RR21 pKa = 11.84MAGRR25 pKa = 11.84HH26 pKa = 5.07AGKK29 pKa = 9.93LALATLALQLWQSRR43 pKa = 11.84RR44 pKa = 11.84TGGAAGANRR53 pKa = 11.84PGRR56 pKa = 11.84RR57 pKa = 11.84WSGRR61 pKa = 11.84RR62 pKa = 11.84PP63 pKa = 3.26

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3072

0

3072

904085

29

1621

294.3

31.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.408 ± 0.074

0.731 ± 0.014

5.906 ± 0.038

6.04 ± 0.041

3.39 ± 0.031

9.149 ± 0.038

1.768 ± 0.019

3.988 ± 0.032

2.363 ± 0.034

10.013 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.228 ± 0.02

2.079 ± 0.028

5.791 ± 0.037

2.93 ± 0.022

8.213 ± 0.052

4.732 ± 0.029

5.025 ± 0.029

7.763 ± 0.038

1.534 ± 0.023

1.95 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski