Lysobacter tolerans
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2386 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N6Y5H5|A0A1N6Y5H5_9GAMM NAD+ diphosphatase OS=Lysobacter tolerans OX=1604334 GN=SAMN05421546_2382 PE=4 SV=1
MM1 pKa = 7.12 EE2 pKa = 4.35 TQIDD6 pKa = 3.93 TAAPGYY12 pKa = 9.14 QQLDD16 pKa = 3.46 APLTFTEE23 pKa = 3.99 AAAAKK28 pKa = 9.81 VRR30 pKa = 11.84 EE31 pKa = 4.6 LINEE35 pKa = 4.13 EE36 pKa = 3.96 GNADD40 pKa = 3.69 LKK42 pKa = 11.17 LRR44 pKa = 11.84 VYY46 pKa = 10.15 IQGGGCSGFQYY57 pKa = 10.65 GFEE60 pKa = 4.0 FDD62 pKa = 3.79 EE63 pKa = 4.64 NQAEE67 pKa = 4.33 DD68 pKa = 4.25 DD69 pKa = 4.3 LAVITNGVTLLVDD82 pKa = 4.09 PLSLQYY88 pKa = 11.38 LMEE91 pKa = 4.22 ATVDD95 pKa = 3.73 YY96 pKa = 11.11 TEE98 pKa = 4.47 TLAGAQFSIRR108 pKa = 11.84 NPNAKK113 pKa = 6.59 TTCGCGSSFTVV124 pKa = 3.3
Molecular weight: 13.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.948
IPC_protein 3.859
Toseland 3.668
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.579
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.973
Patrickios 0.846
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A1N6RQ83|A0A1N6RQ83_9GAMM Uncharacterized protein OS=Lysobacter tolerans OX=1604334 GN=SAMN05421546_1067 PE=4 SV=1
MM1 pKa = 7.33 RR2 pKa = 11.84 TNFVEE7 pKa = 4.4 VRR9 pKa = 11.84 SSRR12 pKa = 11.84 LLKK15 pKa = 10.87 NPLRR19 pKa = 11.84 LYY21 pKa = 10.9 GEE23 pKa = 4.07 YY24 pKa = 10.1 RR25 pKa = 11.84 RR26 pKa = 11.84 PDD28 pKa = 4.22 DD29 pKa = 3.15 KK30 pKa = 11.76 TMIRR34 pKa = 11.84 DD35 pKa = 3.08 VRR37 pKa = 11.84 APYY40 pKa = 11.04 GEE42 pKa = 4.25 TTTIRR47 pKa = 11.84 GDD49 pKa = 3.32 QVTIARR55 pKa = 11.84 AAKK58 pKa = 9.84 SPRR61 pKa = 11.84 TFSMSRR67 pKa = 11.84 APQLAGMQASFGALLSGDD85 pKa = 3.43 EE86 pKa = 4.02 RR87 pKa = 11.84 AIARR91 pKa = 11.84 DD92 pKa = 3.68 FTLTTTGTRR101 pKa = 11.84 AQWRR105 pKa = 11.84 MQMLPKK111 pKa = 10.07 QAALKK116 pKa = 10.39 QYY118 pKa = 10.41 VRR120 pKa = 11.84 DD121 pKa = 3.2 ITLYY125 pKa = 10.72 GRR127 pKa = 11.84 GSEE130 pKa = 4.1 LRR132 pKa = 11.84 CIEE135 pKa = 3.81 TRR137 pKa = 11.84 AVKK140 pKa = 10.63 GEE142 pKa = 3.98 EE143 pKa = 4.06 VQRR146 pKa = 11.84 TLLASAARR154 pKa = 11.84 SVTAATTLDD163 pKa = 4.11 KK164 pKa = 11.19 LVMLCQQGSS173 pKa = 3.22
Molecular weight: 19.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.677
IPC_protein 10.643
Toseland 10.672
ProMoST 10.467
Dawson 10.789
Bjellqvist 10.54
Wikipedia 11.038
Rodwell 10.891
Grimsley 10.847
Solomon 10.935
Lehninger 10.891
Nozaki 10.672
DTASelect 10.54
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.716
Patrickios 10.599
IPC_peptide 10.935
IPC2_peptide 9.633
IPC2.peptide.svr19 8.573
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2386
0
2386
777642
28
2031
325.9
35.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.191 ± 0.067
0.787 ± 0.015
6.117 ± 0.032
5.538 ± 0.044
3.404 ± 0.03
8.398 ± 0.048
2.244 ± 0.029
4.743 ± 0.032
3.414 ± 0.049
10.306 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.594 ± 0.024
2.75 ± 0.036
5.154 ± 0.033
3.682 ± 0.028
7.211 ± 0.046
5.373 ± 0.04
4.983 ± 0.039
7.268 ± 0.041
1.558 ± 0.024
2.286 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here