Lysobacter tolerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2386 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1N6Y5H5|A0A1N6Y5H5_9GAMM NAD+ diphosphatase OS=Lysobacter tolerans OX=1604334 GN=SAMN05421546_2382 PE=4 SV=1
MM1 pKa = 7.12EE2 pKa = 4.35TQIDD6 pKa = 3.93TAAPGYY12 pKa = 9.14QQLDD16 pKa = 3.46APLTFTEE23 pKa = 3.99AAAAKK28 pKa = 9.81VRR30 pKa = 11.84EE31 pKa = 4.6LINEE35 pKa = 4.13EE36 pKa = 3.96GNADD40 pKa = 3.69LKK42 pKa = 11.17LRR44 pKa = 11.84VYY46 pKa = 10.15IQGGGCSGFQYY57 pKa = 10.65GFEE60 pKa = 4.0FDD62 pKa = 3.79EE63 pKa = 4.64NQAEE67 pKa = 4.33DD68 pKa = 4.25DD69 pKa = 4.3LAVITNGVTLLVDD82 pKa = 4.09PLSLQYY88 pKa = 11.38LMEE91 pKa = 4.22ATVDD95 pKa = 3.73YY96 pKa = 11.11TEE98 pKa = 4.47TLAGAQFSIRR108 pKa = 11.84NPNAKK113 pKa = 6.59TTCGCGSSFTVV124 pKa = 3.3

Molecular weight:
13.34 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1N6RQ83|A0A1N6RQ83_9GAMM Uncharacterized protein OS=Lysobacter tolerans OX=1604334 GN=SAMN05421546_1067 PE=4 SV=1
MM1 pKa = 7.33RR2 pKa = 11.84TNFVEE7 pKa = 4.4VRR9 pKa = 11.84SSRR12 pKa = 11.84LLKK15 pKa = 10.87NPLRR19 pKa = 11.84LYY21 pKa = 10.9GEE23 pKa = 4.07YY24 pKa = 10.1RR25 pKa = 11.84RR26 pKa = 11.84PDD28 pKa = 4.22DD29 pKa = 3.15KK30 pKa = 11.76TMIRR34 pKa = 11.84DD35 pKa = 3.08VRR37 pKa = 11.84APYY40 pKa = 11.04GEE42 pKa = 4.25TTTIRR47 pKa = 11.84GDD49 pKa = 3.32QVTIARR55 pKa = 11.84AAKK58 pKa = 9.84SPRR61 pKa = 11.84TFSMSRR67 pKa = 11.84APQLAGMQASFGALLSGDD85 pKa = 3.43EE86 pKa = 4.02RR87 pKa = 11.84AIARR91 pKa = 11.84DD92 pKa = 3.68FTLTTTGTRR101 pKa = 11.84AQWRR105 pKa = 11.84MQMLPKK111 pKa = 10.07QAALKK116 pKa = 10.39QYY118 pKa = 10.41VRR120 pKa = 11.84DD121 pKa = 3.2ITLYY125 pKa = 10.72GRR127 pKa = 11.84GSEE130 pKa = 4.1LRR132 pKa = 11.84CIEE135 pKa = 3.81TRR137 pKa = 11.84AVKK140 pKa = 10.63GEE142 pKa = 3.98EE143 pKa = 4.06VQRR146 pKa = 11.84TLLASAARR154 pKa = 11.84SVTAATTLDD163 pKa = 4.11KK164 pKa = 11.19LVMLCQQGSS173 pKa = 3.22

Molecular weight:
19.36 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2386

0

2386

777642

28

2031

325.9

35.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.191 ± 0.067

0.787 ± 0.015

6.117 ± 0.032

5.538 ± 0.044

3.404 ± 0.03

8.398 ± 0.048

2.244 ± 0.029

4.743 ± 0.032

3.414 ± 0.049

10.306 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.024

2.75 ± 0.036

5.154 ± 0.033

3.682 ± 0.028

7.211 ± 0.046

5.373 ± 0.04

4.983 ± 0.039

7.268 ± 0.041

1.558 ± 0.024

2.286 ± 0.026

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski