Candidatus Tokpelaia sp.

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiales incertae sedis; Candidatus Tokpelaia; unclassified Candidatus Tokpelaia

Average proteome isoelectric point is 7.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1880 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5M8PBS3|A0A5M8PBS3_9RHIZ Uncharacterized protein OS=Candidatus Tokpelaia sp. OX=2233777 GN=DPQ22_00765 PE=4 SV=1
MM1 pKa = 7.45TYY3 pKa = 10.23IVTDD7 pKa = 3.09NCIRR11 pKa = 11.84CKK13 pKa = 10.23YY14 pKa = 7.51TDD16 pKa = 3.93CVEE19 pKa = 4.15VCPVDD24 pKa = 3.79CFYY27 pKa = 11.19EE28 pKa = 4.58GEE30 pKa = 4.18NMLVINPDD38 pKa = 2.97EE39 pKa = 5.49CIDD42 pKa = 4.08CGVCEE47 pKa = 4.44PEE49 pKa = 5.05CPAEE53 pKa = 4.42AIKK56 pKa = 10.65PDD58 pKa = 3.94TEE60 pKa = 4.65PGLEE64 pKa = 3.98KK65 pKa = 10.1WLEE68 pKa = 4.0LNAEE72 pKa = 4.55YY73 pKa = 10.26AAKK76 pKa = 9.47WPNITVKK83 pKa = 10.6KK84 pKa = 10.71AEE86 pKa = 4.19LPEE89 pKa = 3.97ARR91 pKa = 11.84QMDD94 pKa = 4.02GVEE97 pKa = 4.92DD98 pKa = 3.89KK99 pKa = 10.58FAQYY103 pKa = 10.02FSPKK107 pKa = 9.22PGSGDD112 pKa = 3.1

Molecular weight: 12.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5M8P8A5|A0A5M8P8A5_9RHIZ Uncharacterized protein OS=Candidatus Tokpelaia sp. OX=2233777 GN=DPQ22_05260 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.48GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVAGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1880

0

1880

542457

41

4251

288.5

31.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.663 ± 0.092

1.095 ± 0.024

4.801 ± 0.054

5.527 ± 0.069

4.198 ± 0.046

7.869 ± 0.059

1.922 ± 0.026

6.244 ± 0.043

5.144 ± 0.06

9.983 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.309 ± 0.037

3.673 ± 0.055

4.688 ± 0.048

3.976 ± 0.037

6.474 ± 0.065

5.28 ± 0.05

4.529 ± 0.104

5.668 ± 0.05

1.144 ± 0.024

2.812 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski