Streptomyces phage CherryBlossom

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rimavirus; unclassified Rimavirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6D8I6|A0A5J6D8I6_9CAUD Hydrolase OS=Streptomyces phage CherryBlossom OX=2601687 GN=27 PE=4 SV=1
MM1 pKa = 7.24TQLTWDD7 pKa = 3.45KK8 pKa = 11.14SGEE11 pKa = 4.07RR12 pKa = 11.84YY13 pKa = 9.81YY14 pKa = 10.45EE15 pKa = 3.85TGVDD19 pKa = 3.65HH20 pKa = 6.89GVLYY24 pKa = 10.48IPNSQGDD31 pKa = 4.07YY32 pKa = 8.79TSGYY36 pKa = 9.07AWNGLTTVTEE46 pKa = 4.44SPSGAEE52 pKa = 4.33SNPQYY57 pKa = 11.32ADD59 pKa = 3.31NIKK62 pKa = 10.1YY63 pKa = 10.68LNLISAEE70 pKa = 4.08EE71 pKa = 3.89FGGTIEE77 pKa = 4.71AFTYY81 pKa = 10.2PDD83 pKa = 3.98EE84 pKa = 5.39FGQCDD89 pKa = 3.72GTAAPTPGVLVGQQSRR105 pKa = 11.84KK106 pKa = 7.76TFGLSYY112 pKa = 9.42RR113 pKa = 11.84TKK115 pKa = 10.7LGNDD119 pKa = 3.95LDD121 pKa = 4.55GQDD124 pKa = 4.02HH125 pKa = 7.09GYY127 pKa = 10.58KK128 pKa = 10.3LHH130 pKa = 6.48LVYY133 pKa = 10.45GALAAPSEE141 pKa = 4.28KK142 pKa = 10.51AYY144 pKa = 9.79ATVNDD149 pKa = 4.08SPEE152 pKa = 4.78AITFSWEE159 pKa = 3.78FTTTPVEE166 pKa = 4.21VGQIAGVDD174 pKa = 3.7YY175 pKa = 10.95KK176 pKa = 10.33PTATLTIDD184 pKa = 3.51STKK187 pKa = 10.49VDD189 pKa = 3.76AGALDD194 pKa = 3.67TLEE197 pKa = 4.08EE198 pKa = 4.13FLYY201 pKa = 9.09GTEE204 pKa = 4.23GTDD207 pKa = 3.99PSLPDD212 pKa = 4.04PATVIAMFSGTVLEE226 pKa = 4.44ATPTEE231 pKa = 4.16PSYY234 pKa = 11.5DD235 pKa = 3.47AATDD239 pKa = 4.19TITIPTVTGVNYY251 pKa = 10.58YY252 pKa = 10.04IGGEE256 pKa = 4.07LQAPGPVIISEE267 pKa = 4.15DD268 pKa = 3.98TIVEE272 pKa = 3.87ARR274 pKa = 11.84PAKK277 pKa = 10.23GYY279 pKa = 10.35KK280 pKa = 10.01FPANVDD286 pKa = 3.55NDD288 pKa = 3.75WLITYY293 pKa = 8.72SS294 pKa = 3.4

Molecular weight: 31.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6D7Z9|A0A5J6D7Z9_9CAUD Minor tail protein OS=Streptomyces phage CherryBlossom OX=2601687 GN=24 PE=4 SV=1
MM1 pKa = 7.19EE2 pKa = 4.61TFISDD7 pKa = 4.77FNWLWTYY14 pKa = 9.12FVIIGAAFAILGVWKK29 pKa = 10.46LIDD32 pKa = 4.37EE33 pKa = 4.23IRR35 pKa = 11.84LYY37 pKa = 8.92TEE39 pKa = 3.41RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84YY43 pKa = 9.29RR44 pKa = 11.84RR45 pKa = 11.84ARR47 pKa = 11.84AEE49 pKa = 4.01RR50 pKa = 11.84TGGTWAKK57 pKa = 10.33KK58 pKa = 8.34STGEE62 pKa = 3.69ISAGSLRR69 pKa = 11.84SSGDD73 pKa = 2.91RR74 pKa = 3.52

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

17293

44

1828

201.1

22.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.154 ± 0.548

0.59 ± 0.119

6.257 ± 0.34

7.367 ± 0.345

3.551 ± 0.184

7.356 ± 0.465

1.903 ± 0.226

5.378 ± 0.178

6.945 ± 0.459

7.94 ± 0.444

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.729 ± 0.146

4.007 ± 0.166

4.14 ± 0.329

3.406 ± 0.189

5.187 ± 0.38

5.956 ± 0.249

6.529 ± 0.337

6.806 ± 0.201

1.44 ± 0.133

3.36 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski