Amino acid dipepetide frequency for Streptomyces phage CherryBlossom

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.641AlaAla: 10.641 ± 1.605
0.231AlaCys: 0.231 ± 0.122
5.031AlaAsp: 5.031 ± 0.747
7.171AlaGlu: 7.171 ± 0.674
2.545AlaPhe: 2.545 ± 0.342
6.997AlaGly: 6.997 ± 1.12
1.446AlaHis: 1.446 ± 0.356
4.742AlaIle: 4.742 ± 0.477
6.882AlaLys: 6.882 ± 0.734
8.501AlaLeu: 8.501 ± 1.399
4.048AlaMet: 4.048 ± 0.589
2.834AlaAsn: 2.834 ± 0.374
3.065AlaPro: 3.065 ± 0.424
3.47AlaGln: 3.47 ± 0.457
4.453AlaArg: 4.453 ± 0.518
3.817AlaSer: 3.817 ± 0.521
5.725AlaThr: 5.725 ± 0.548
5.205AlaVal: 5.205 ± 0.482
1.272AlaTrp: 1.272 ± 0.288
3.47AlaTyr: 3.47 ± 0.411
0.0AlaXaa: 0.0 ± 0.0
Cys
0.463CysAla: 0.463 ± 0.189
0.173CysCys: 0.173 ± 0.112
0.347CysAsp: 0.347 ± 0.153
0.405CysGlu: 0.405 ± 0.152
0.231CysPhe: 0.231 ± 0.109
0.463CysGly: 0.463 ± 0.165
0.116CysHis: 0.116 ± 0.091
0.231CysIle: 0.231 ± 0.11
0.173CysLys: 0.173 ± 0.093
0.752CysLeu: 0.752 ± 0.251
0.116CysMet: 0.116 ± 0.078
0.289CysAsn: 0.289 ± 0.148
0.347CysPro: 0.347 ± 0.18
0.173CysGln: 0.173 ± 0.094
0.173CysArg: 0.173 ± 0.098
0.405CysSer: 0.405 ± 0.158
0.347CysThr: 0.347 ± 0.116
0.347CysVal: 0.347 ± 0.159
0.173CysTrp: 0.173 ± 0.093
0.173CysTyr: 0.173 ± 0.096
0.0CysXaa: 0.0 ± 0.0
Asp
5.494AspAla: 5.494 ± 0.58
0.463AspCys: 0.463 ± 0.154
3.99AspAsp: 3.99 ± 0.711
6.072AspGlu: 6.072 ± 0.785
2.718AspPhe: 2.718 ± 0.545
5.378AspGly: 5.378 ± 0.63
0.925AspHis: 0.925 ± 0.271
3.759AspIle: 3.759 ± 0.569
3.007AspLys: 3.007 ± 0.433
4.8AspLeu: 4.8 ± 0.504
1.619AspMet: 1.619 ± 0.273
2.371AspAsn: 2.371 ± 0.474
3.99AspPro: 3.99 ± 0.627
1.793AspGln: 1.793 ± 0.42
3.412AspArg: 3.412 ± 0.499
3.065AspSer: 3.065 ± 0.364
3.181AspThr: 3.181 ± 0.426
3.123AspVal: 3.123 ± 0.439
1.735AspTrp: 1.735 ± 0.333
1.677AspTyr: 1.677 ± 0.31
0.0AspXaa: 0.0 ± 0.0
Glu
5.667GluAla: 5.667 ± 0.602
0.289GluCys: 0.289 ± 0.14
5.089GluAsp: 5.089 ± 0.649
6.419GluGlu: 6.419 ± 0.802
3.47GluPhe: 3.47 ± 0.529
5.205GluGly: 5.205 ± 0.544
1.157GluHis: 1.157 ± 0.325
4.048GluIle: 4.048 ± 0.456
4.916GluLys: 4.916 ± 0.408
6.303GluLeu: 6.303 ± 0.621
2.834GluMet: 2.834 ± 0.309
2.949GluAsn: 2.949 ± 0.41
2.66GluPro: 2.66 ± 0.461
2.66GluGln: 2.66 ± 0.355
4.337GluArg: 4.337 ± 0.645
3.47GluSer: 3.47 ± 0.463
3.817GluThr: 3.817 ± 0.482
5.436GluVal: 5.436 ± 0.589
1.041GluTrp: 1.041 ± 0.197
1.908GluTyr: 1.908 ± 0.356
0.0GluXaa: 0.0 ± 0.0
Phe
3.238PheAla: 3.238 ± 0.334
0.347PheCys: 0.347 ± 0.14
3.181PheAsp: 3.181 ± 0.445
2.718PheGlu: 2.718 ± 0.413
1.561PhePhe: 1.561 ± 0.25
2.776PheGly: 2.776 ± 0.478
0.405PheHis: 0.405 ± 0.149
1.793PheIle: 1.793 ± 0.386
2.082PheLys: 2.082 ± 0.399
2.14PheLeu: 2.14 ± 0.412
0.81PheMet: 0.81 ± 0.187
1.677PheAsn: 1.677 ± 0.252
1.157PhePro: 1.157 ± 0.215
1.446PheGln: 1.446 ± 0.281
2.487PheArg: 2.487 ± 0.326
1.793PheSer: 1.793 ± 0.41
2.313PheThr: 2.313 ± 0.462
2.082PheVal: 2.082 ± 0.423
0.578PheTrp: 0.578 ± 0.194
0.925PheTyr: 0.925 ± 0.256
0.0PheXaa: 0.0 ± 0.0
Gly
6.303GlyAla: 6.303 ± 0.821
0.463GlyCys: 0.463 ± 0.178
4.626GlyAsp: 4.626 ± 0.467
4.048GlyGlu: 4.048 ± 0.541
3.296GlyPhe: 3.296 ± 0.66
5.031GlyGly: 5.031 ± 0.624
1.099GlyHis: 1.099 ± 0.274
4.395GlyIle: 4.395 ± 0.853
4.395GlyLys: 4.395 ± 0.55
5.957GlyLeu: 5.957 ± 1.475
2.255GlyMet: 2.255 ± 0.329
2.776GlyAsn: 2.776 ± 0.443
2.487GlyPro: 2.487 ± 0.412
1.793GlyGln: 1.793 ± 0.392
3.007GlyArg: 3.007 ± 0.327
4.8GlySer: 4.8 ± 0.63
6.246GlyThr: 6.246 ± 0.574
6.361GlyVal: 6.361 ± 0.653
1.041GlyTrp: 1.041 ± 0.227
3.181GlyTyr: 3.181 ± 0.574
0.0GlyXaa: 0.0 ± 0.0
His
1.099HisAla: 1.099 ± 0.335
0.231HisCys: 0.231 ± 0.132
1.272HisAsp: 1.272 ± 0.304
1.619HisGlu: 1.619 ± 0.331
0.405HisPhe: 0.405 ± 0.18
1.561HisGly: 1.561 ± 0.403
0.578HisHis: 0.578 ± 0.157
1.214HisIle: 1.214 ± 0.297
1.272HisLys: 1.272 ± 0.282
1.388HisLeu: 1.388 ± 0.314
0.463HisMet: 0.463 ± 0.16
0.463HisAsn: 0.463 ± 0.18
0.867HisPro: 0.867 ± 0.223
0.636HisGln: 0.636 ± 0.226
0.925HisArg: 0.925 ± 0.229
1.272HisSer: 1.272 ± 0.239
0.578HisThr: 0.578 ± 0.188
1.504HisVal: 1.504 ± 0.31
0.289HisTrp: 0.289 ± 0.157
1.388HisTyr: 1.388 ± 0.278
0.0HisXaa: 0.0 ± 0.0
Ile
4.916IleAla: 4.916 ± 0.431
0.463IleCys: 0.463 ± 0.18
4.164IleAsp: 4.164 ± 0.526
4.511IleGlu: 4.511 ± 0.409
2.082IlePhe: 2.082 ± 0.326
3.643IleGly: 3.643 ± 0.77
1.214IleHis: 1.214 ± 0.338
2.429IleIle: 2.429 ± 0.467
3.238IleLys: 3.238 ± 0.419
4.684IleLeu: 4.684 ± 0.47
0.925IleMet: 0.925 ± 0.206
2.14IleAsn: 2.14 ± 0.318
2.371IlePro: 2.371 ± 0.326
1.851IleGln: 1.851 ± 0.314
3.007IleArg: 3.007 ± 0.404
3.585IleSer: 3.585 ± 0.524
3.238IleThr: 3.238 ± 0.38
3.412IleVal: 3.412 ± 0.404
0.289IleTrp: 0.289 ± 0.141
1.619IleTyr: 1.619 ± 0.241
0.0IleXaa: 0.0 ± 0.0
Lys
6.65LysAla: 6.65 ± 0.793
0.173LysCys: 0.173 ± 0.093
3.701LysAsp: 3.701 ± 0.539
3.817LysGlu: 3.817 ± 0.65
1.793LysPhe: 1.793 ± 0.321
4.973LysGly: 4.973 ± 0.852
1.33LysHis: 1.33 ± 0.317
3.701LysIle: 3.701 ± 0.414
7.287LysLys: 7.287 ± 0.939
6.65LysLeu: 6.65 ± 0.714
1.908LysMet: 1.908 ± 0.488
2.776LysAsn: 2.776 ± 0.459
2.429LysPro: 2.429 ± 0.525
2.487LysGln: 2.487 ± 0.354
3.238LysArg: 3.238 ± 0.574
4.742LysSer: 4.742 ± 0.545
4.395LysThr: 4.395 ± 0.567
4.222LysVal: 4.222 ± 0.532
0.81LysTrp: 0.81 ± 0.258
2.371LysTyr: 2.371 ± 0.284
0.0LysXaa: 0.0 ± 0.0
Leu
7.576LeuAla: 7.576 ± 1.09
0.463LeuCys: 0.463 ± 0.15
4.858LeuAsp: 4.858 ± 0.538
6.477LeuGlu: 6.477 ± 0.498
2.371LeuPhe: 2.371 ± 0.427
5.957LeuGly: 5.957 ± 1.093
1.735LeuHis: 1.735 ± 0.306
4.048LeuIle: 4.048 ± 0.629
6.246LeuLys: 6.246 ± 0.574
5.436LeuLeu: 5.436 ± 0.781
2.024LeuMet: 2.024 ± 0.33
3.123LeuAsn: 3.123 ± 0.457
2.487LeuPro: 2.487 ± 0.381
3.007LeuGln: 3.007 ± 0.472
4.048LeuArg: 4.048 ± 0.401
4.279LeuSer: 4.279 ± 0.463
6.072LeuThr: 6.072 ± 0.641
5.899LeuVal: 5.899 ± 0.722
0.867LeuTrp: 0.867 ± 0.183
2.429LeuTyr: 2.429 ± 0.31
0.0LeuXaa: 0.0 ± 0.0
Met
2.313MetAla: 2.313 ± 0.331
0.116MetCys: 0.116 ± 0.078
2.198MetAsp: 2.198 ± 0.239
1.157MetGlu: 1.157 ± 0.259
1.388MetPhe: 1.388 ± 0.268
1.388MetGly: 1.388 ± 0.225
0.289MetHis: 0.289 ± 0.116
1.33MetIle: 1.33 ± 0.365
1.619MetLys: 1.619 ± 0.258
3.007MetLeu: 3.007 ± 0.48
0.405MetMet: 0.405 ± 0.176
1.561MetAsn: 1.561 ± 0.277
1.446MetPro: 1.446 ± 0.289
0.636MetGln: 0.636 ± 0.168
1.504MetArg: 1.504 ± 0.266
2.14MetSer: 2.14 ± 0.353
2.487MetThr: 2.487 ± 0.387
1.157MetVal: 1.157 ± 0.237
0.405MetTrp: 0.405 ± 0.13
0.752MetTyr: 0.752 ± 0.206
0.0MetXaa: 0.0 ± 0.0
Asn
3.47AsnAla: 3.47 ± 0.357
0.347AsnCys: 0.347 ± 0.166
2.429AsnAsp: 2.429 ± 0.343
3.181AsnGlu: 3.181 ± 0.443
0.752AsnPhe: 0.752 ± 0.204
3.065AsnGly: 3.065 ± 0.418
1.214AsnHis: 1.214 ± 0.295
1.908AsnIle: 1.908 ± 0.234
2.545AsnLys: 2.545 ± 0.349
2.371AsnLeu: 2.371 ± 0.547
0.983AsnMet: 0.983 ± 0.234
1.966AsnAsn: 1.966 ± 0.342
2.371AsnPro: 2.371 ± 0.411
1.388AsnGln: 1.388 ± 0.279
2.545AsnArg: 2.545 ± 0.465
2.776AsnSer: 2.776 ± 0.4
2.487AsnThr: 2.487 ± 0.338
2.718AsnVal: 2.718 ± 0.369
0.289AsnTrp: 0.289 ± 0.126
1.272AsnTyr: 1.272 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
3.296ProAla: 3.296 ± 0.434
0.405ProCys: 0.405 ± 0.172
3.643ProAsp: 3.643 ± 0.654
3.47ProGlu: 3.47 ± 0.532
1.561ProPhe: 1.561 ± 0.306
2.892ProGly: 2.892 ± 0.503
0.694ProHis: 0.694 ± 0.212
1.793ProIle: 1.793 ± 0.239
3.238ProLys: 3.238 ± 0.389
2.371ProLeu: 2.371 ± 0.373
0.925ProMet: 0.925 ± 0.26
1.561ProAsn: 1.561 ± 0.288
2.024ProPro: 2.024 ± 0.446
1.041ProGln: 1.041 ± 0.245
2.024ProArg: 2.024 ± 0.47
2.776ProSer: 2.776 ± 0.422
2.834ProThr: 2.834 ± 0.493
3.354ProVal: 3.354 ± 0.432
0.405ProTrp: 0.405 ± 0.164
1.099ProTyr: 1.099 ± 0.263
0.0ProXaa: 0.0 ± 0.0
Gln
3.181GlnAla: 3.181 ± 0.434
0.289GlnCys: 0.289 ± 0.138
1.851GlnAsp: 1.851 ± 0.346
2.14GlnGlu: 2.14 ± 0.389
1.041GlnPhe: 1.041 ± 0.201
2.14GlnGly: 2.14 ± 0.408
0.867GlnHis: 0.867 ± 0.272
2.198GlnIle: 2.198 ± 0.353
2.371GlnLys: 2.371 ± 0.357
2.776GlnLeu: 2.776 ± 0.543
0.81GlnMet: 0.81 ± 0.216
1.33GlnAsn: 1.33 ± 0.311
1.214GlnPro: 1.214 ± 0.309
1.793GlnGln: 1.793 ± 0.535
1.677GlnArg: 1.677 ± 0.254
2.198GlnSer: 2.198 ± 0.329
1.851GlnThr: 1.851 ± 0.352
2.718GlnVal: 2.718 ± 0.414
0.405GlnTrp: 0.405 ± 0.142
1.214GlnTyr: 1.214 ± 0.259
0.0GlnXaa: 0.0 ± 0.0
Arg
4.106ArgAla: 4.106 ± 0.613
0.405ArgCys: 0.405 ± 0.142
2.834ArgAsp: 2.834 ± 0.43
3.528ArgGlu: 3.528 ± 0.492
0.925ArgPhe: 0.925 ± 0.167
3.238ArgGly: 3.238 ± 0.494
1.157ArgHis: 1.157 ± 0.303
2.718ArgIle: 2.718 ± 0.443
4.395ArgLys: 4.395 ± 0.553
4.8ArgLeu: 4.8 ± 0.735
1.677ArgMet: 1.677 ± 0.284
2.024ArgAsn: 2.024 ± 0.353
2.313ArgPro: 2.313 ± 0.363
2.198ArgGln: 2.198 ± 0.377
4.048ArgArg: 4.048 ± 0.649
3.296ArgSer: 3.296 ± 0.448
3.123ArgThr: 3.123 ± 0.416
2.892ArgVal: 2.892 ± 0.395
0.752ArgTrp: 0.752 ± 0.248
1.446ArgTyr: 1.446 ± 0.312
0.0ArgXaa: 0.0 ± 0.0
Ser
5.725SerAla: 5.725 ± 0.749
0.231SerCys: 0.231 ± 0.094
3.354SerAsp: 3.354 ± 0.467
4.626SerGlu: 4.626 ± 0.446
2.429SerPhe: 2.429 ± 0.438
4.973SerGly: 4.973 ± 0.542
1.099SerHis: 1.099 ± 0.267
2.949SerIle: 2.949 ± 0.461
3.99SerLys: 3.99 ± 0.694
4.453SerLeu: 4.453 ± 0.551
1.388SerMet: 1.388 ± 0.221
2.602SerAsn: 2.602 ± 0.427
2.892SerPro: 2.892 ± 0.433
1.908SerGln: 1.908 ± 0.313
2.545SerArg: 2.545 ± 0.433
4.048SerSer: 4.048 ± 0.74
3.759SerThr: 3.759 ± 0.49
3.817SerVal: 3.817 ± 0.507
0.983SerTrp: 0.983 ± 0.207
1.735SerTyr: 1.735 ± 0.358
0.0SerXaa: 0.0 ± 0.0
Thr
6.072ThrAla: 6.072 ± 0.676
0.173ThrCys: 0.173 ± 0.114
3.47ThrAsp: 3.47 ± 0.345
4.164ThrGlu: 4.164 ± 0.559
2.66ThrPhe: 2.66 ± 0.297
5.667ThrGly: 5.667 ± 0.592
1.561ThrHis: 1.561 ± 0.249
4.222ThrIle: 4.222 ± 0.498
3.932ThrLys: 3.932 ± 0.378
4.916ThrLeu: 4.916 ± 0.55
1.504ThrMet: 1.504 ± 0.272
1.33ThrAsn: 1.33 ± 0.236
3.181ThrPro: 3.181 ± 0.365
1.561ThrGln: 1.561 ± 0.311
2.892ThrArg: 2.892 ± 0.363
3.759ThrSer: 3.759 ± 0.634
5.436ThrThr: 5.436 ± 0.749
5.667ThrVal: 5.667 ± 0.621
1.041ThrTrp: 1.041 ± 0.273
2.082ThrTyr: 2.082 ± 0.408
0.0ThrXaa: 0.0 ± 0.0
Val
7.229ValAla: 7.229 ± 0.686
0.347ValCys: 0.347 ± 0.155
3.528ValAsp: 3.528 ± 0.423
4.569ValGlu: 4.569 ± 0.653
2.198ValPhe: 2.198 ± 0.436
4.916ValGly: 4.916 ± 0.567
1.041ValHis: 1.041 ± 0.282
3.759ValIle: 3.759 ± 0.427
4.973ValLys: 4.973 ± 0.479
5.263ValLeu: 5.263 ± 0.592
1.561ValMet: 1.561 ± 0.353
3.123ValAsn: 3.123 ± 0.334
2.545ValPro: 2.545 ± 0.422
1.908ValGln: 1.908 ± 0.303
3.412ValArg: 3.412 ± 0.477
3.759ValSer: 3.759 ± 0.478
5.031ValThr: 5.031 ± 0.62
4.626ValVal: 4.626 ± 0.624
1.446ValTrp: 1.446 ± 0.292
2.834ValTyr: 2.834 ± 0.535
0.0ValXaa: 0.0 ± 0.0
Trp
1.214TrpAla: 1.214 ± 0.267
0.116TrpCys: 0.116 ± 0.071
1.157TrpAsp: 1.157 ± 0.303
1.33TrpGlu: 1.33 ± 0.251
0.405TrpPhe: 0.405 ± 0.18
1.446TrpGly: 1.446 ± 0.265
0.347TrpHis: 0.347 ± 0.142
0.463TrpIle: 0.463 ± 0.135
0.867TrpLys: 0.867 ± 0.269
0.81TrpLeu: 0.81 ± 0.208
0.636TrpMet: 0.636 ± 0.207
1.157TrpAsn: 1.157 ± 0.333
0.231TrpPro: 0.231 ± 0.099
0.405TrpGln: 0.405 ± 0.139
0.347TrpArg: 0.347 ± 0.153
1.041TrpSer: 1.041 ± 0.275
0.752TrpThr: 0.752 ± 0.193
1.099TrpVal: 1.099 ± 0.321
0.116TrpTrp: 0.116 ± 0.073
0.463TrpTyr: 0.463 ± 0.18
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.892TyrAla: 2.892 ± 0.618
0.173TyrCys: 0.173 ± 0.091
1.851TyrAsp: 1.851 ± 0.311
2.255TyrGlu: 2.255 ± 0.323
1.677TyrPhe: 1.677 ± 0.355
1.851TyrGly: 1.851 ± 0.388
0.752TyrHis: 0.752 ± 0.252
2.082TyrIle: 2.082 ± 0.404
2.024TyrLys: 2.024 ± 0.446
2.024TyrLeu: 2.024 ± 0.313
0.347TyrMet: 0.347 ± 0.154
2.024TyrAsn: 2.024 ± 0.396
1.33TyrPro: 1.33 ± 0.288
1.908TyrGln: 1.908 ± 0.337
1.793TyrArg: 1.793 ± 0.351
2.545TyrSer: 2.545 ± 0.439
1.619TyrThr: 1.619 ± 0.34
2.429TyrVal: 2.429 ± 0.331
0.463TyrTrp: 0.463 ± 0.202
1.561TyrTyr: 1.561 ± 0.394
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (17293 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski