Spiribacter sp. C176

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Spiribacter; unclassified Spiribacter

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2078 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N7QTI3|A0A6N7QTI3_9GAMM NADP(H)-dependent aldo-keto reductase OS=Spiribacter sp. C176 OX=2664894 GN=GH984_09280 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.18KK3 pKa = 10.45VYY5 pKa = 9.87LAAAATSALALAATPVMADD24 pKa = 3.29DD25 pKa = 4.25HH26 pKa = 8.71ASMSVYY32 pKa = 10.45GWITAEE38 pKa = 4.02VQDD41 pKa = 5.91NGTEE45 pKa = 3.77WDD47 pKa = 3.31ATGNGNSPSRR57 pKa = 11.84FGIRR61 pKa = 11.84GSRR64 pKa = 11.84EE65 pKa = 3.46FGAVTGDD72 pKa = 3.14AQLEE76 pKa = 4.36YY77 pKa = 10.8GVTKK81 pKa = 10.67AQGEE85 pKa = 4.71GPGIRR90 pKa = 11.84LANAALSGDD99 pKa = 3.79FGSVRR104 pKa = 11.84IGSQWNPGYY113 pKa = 10.85LWTTATTDD121 pKa = 3.15VFTSGAYY128 pKa = 8.73TGARR132 pKa = 11.84STDD135 pKa = 3.61FVFRR139 pKa = 11.84QDD141 pKa = 3.15GAIFYY146 pKa = 7.1YY147 pKa = 10.63APSLGGLDD155 pKa = 3.13IAVGGNMLASDD166 pKa = 4.46DD167 pKa = 4.45SSDD170 pKa = 3.86EE171 pKa = 4.0FDD173 pKa = 3.5TMTVSARR180 pKa = 11.84YY181 pKa = 9.82SFGDD185 pKa = 3.79LYY187 pKa = 11.75VSATYY192 pKa = 9.85MEE194 pKa = 5.42ADD196 pKa = 4.28DD197 pKa = 4.85EE198 pKa = 4.5NSSEE202 pKa = 4.06ATAFAASYY210 pKa = 10.49DD211 pKa = 3.57FGMFTLAGSMSDD223 pKa = 3.18NDD225 pKa = 4.13GVGSYY230 pKa = 10.8NDD232 pKa = 3.22NGTPYY237 pKa = 10.21EE238 pKa = 4.32VVATVSATEE247 pKa = 3.89EE248 pKa = 4.19LTLKK252 pKa = 10.55AAYY255 pKa = 8.46TDD257 pKa = 3.83RR258 pKa = 11.84DD259 pKa = 3.86LEE261 pKa = 5.59AGDD264 pKa = 4.47DD265 pKa = 3.54SSMAAEE271 pKa = 3.83AAYY274 pKa = 10.45AFGSGVTGFAGFSSSDD290 pKa = 3.29DD291 pKa = 4.23GINDD295 pKa = 3.85GEE297 pKa = 5.1DD298 pKa = 2.93ILSFGVQVVFF308 pKa = 4.98

Molecular weight: 32.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N7QPK6|A0A6N7QPK6_9GAMM UPF0260 protein GH984_01090 OS=Spiribacter sp. C176 OX=2664894 GN=GH984_01090 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.52RR12 pKa = 11.84KK13 pKa = 8.13RR14 pKa = 11.84THH16 pKa = 6.01GFRR19 pKa = 11.84SRR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.79ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.38GRR39 pKa = 11.84ARR41 pKa = 11.84LAPP44 pKa = 4.03

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2078

0

2078

708737

26

11782

341.1

37.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.525 ± 0.073

0.856 ± 0.031

5.732 ± 0.094

6.106 ± 0.065

3.451 ± 0.043

8.314 ± 0.176

2.192 ± 0.048

5.447 ± 0.039

2.65 ± 0.053

10.795 ± 0.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.282 ± 0.053

3.039 ± 0.077

4.788 ± 0.093

4.228 ± 0.048

6.658 ± 0.12

5.541 ± 0.095

5.377 ± 0.148

7.313 ± 0.052

1.363 ± 0.03

2.344 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski