Amino acid dipepetide frequency for Spiribacter sp. C176

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.406AlaAla: 14.406 ± 0.237
0.961AlaCys: 0.961 ± 0.046
6.833AlaAsp: 6.833 ± 0.152
8.071AlaGlu: 8.071 ± 0.137
3.739AlaPhe: 3.739 ± 0.092
10.153AlaGly: 10.153 ± 0.239
2.29AlaHis: 2.29 ± 0.074
6.228AlaIle: 6.228 ± 0.095
3.032AlaLys: 3.032 ± 0.071
13.722AlaLeu: 13.722 ± 0.198
2.891AlaMet: 2.891 ± 0.079
3.259AlaAsn: 3.259 ± 0.112
4.32AlaPro: 4.32 ± 0.104
4.742AlaGln: 4.742 ± 0.099
6.996AlaArg: 6.996 ± 0.151
5.829AlaSer: 5.829 ± 0.153
5.486AlaThr: 5.486 ± 0.182
8.459AlaVal: 8.459 ± 0.117
1.466AlaTrp: 1.466 ± 0.057
2.368AlaTyr: 2.368 ± 0.068
0.0AlaXaa: 0.0 ± 0.0
Cys
0.842CysAla: 0.842 ± 0.043
0.137CysCys: 0.137 ± 0.013
0.498CysAsp: 0.498 ± 0.034
0.442CysGlu: 0.442 ± 0.027
0.317CysPhe: 0.317 ± 0.023
0.808CysGly: 0.808 ± 0.044
0.269CysHis: 0.269 ± 0.024
0.446CysIle: 0.446 ± 0.031
0.178CysLys: 0.178 ± 0.019
0.934CysLeu: 0.934 ± 0.04
0.157CysMet: 0.157 ± 0.017
0.267CysAsn: 0.267 ± 0.021
0.488CysPro: 0.488 ± 0.034
0.387CysGln: 0.387 ± 0.026
0.56CysArg: 0.56 ± 0.032
0.461CysSer: 0.461 ± 0.028
0.396CysThr: 0.396 ± 0.026
0.598CysVal: 0.598 ± 0.04
0.119CysTrp: 0.119 ± 0.015
0.257CysTyr: 0.257 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
6.554AspAla: 6.554 ± 0.153
0.552AspCys: 0.552 ± 0.034
3.194AspAsp: 3.194 ± 0.088
3.75AspGlu: 3.75 ± 0.08
2.16AspPhe: 2.16 ± 0.056
4.77AspGly: 4.77 ± 0.22
1.173AspHis: 1.173 ± 0.038
3.234AspIle: 3.234 ± 0.107
1.232AspLys: 1.232 ± 0.045
5.946AspLeu: 5.946 ± 0.143
1.101AspMet: 1.101 ± 0.044
1.573AspAsn: 1.573 ± 0.082
3.08AspPro: 3.08 ± 0.07
2.596AspGln: 2.596 ± 0.066
3.748AspArg: 3.748 ± 0.081
2.819AspSer: 2.819 ± 0.129
2.986AspThr: 2.986 ± 0.132
4.033AspVal: 4.033 ± 0.13
1.053AspTrp: 1.053 ± 0.041
1.764AspTyr: 1.764 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
7.67GluAla: 7.67 ± 0.144
0.394GluCys: 0.394 ± 0.027
2.902GluAsp: 2.902 ± 0.074
3.145GluGlu: 3.145 ± 0.096
1.857GluPhe: 1.857 ± 0.048
4.215GluGly: 4.215 ± 0.095
1.449GluHis: 1.449 ± 0.062
3.43GluIle: 3.43 ± 0.074
1.985GluLys: 1.985 ± 0.079
6.15GluLeu: 6.15 ± 0.101
1.544GluMet: 1.544 ± 0.061
1.668GluAsn: 1.668 ± 0.056
2.905GluPro: 2.905 ± 0.079
3.652GluGln: 3.652 ± 0.106
5.139GluArg: 5.139 ± 0.125
3.224GluSer: 3.224 ± 0.08
3.145GluThr: 3.145 ± 0.081
4.491GluVal: 4.491 ± 0.086
0.859GluTrp: 0.859 ± 0.039
1.24GluTyr: 1.24 ± 0.041
0.0GluXaa: 0.0 ± 0.0
Phe
3.714PheAla: 3.714 ± 0.072
0.388PheCys: 0.388 ± 0.028
2.355PheAsp: 2.355 ± 0.062
2.228PheGlu: 2.228 ± 0.065
1.425PhePhe: 1.425 ± 0.061
3.007PheGly: 3.007 ± 0.082
0.756PheHis: 0.756 ± 0.038
1.889PheIle: 1.889 ± 0.064
0.869PheLys: 0.869 ± 0.042
3.278PheLeu: 3.278 ± 0.115
0.751PheMet: 0.751 ± 0.041
1.307PheAsn: 1.307 ± 0.048
1.362PhePro: 1.362 ± 0.061
1.074PheGln: 1.074 ± 0.042
1.831PheArg: 1.831 ± 0.062
2.344PheSer: 2.344 ± 0.062
1.949PheThr: 1.949 ± 0.07
2.486PheVal: 2.486 ± 0.067
0.536PheTrp: 0.536 ± 0.032
0.959PheTyr: 0.959 ± 0.04
0.0PheXaa: 0.0 ± 0.0
Gly
8.058GlyAla: 8.058 ± 0.253
0.827GlyCys: 0.827 ± 0.039
4.783GlyAsp: 4.783 ± 0.318
4.779GlyGlu: 4.779 ± 0.096
3.242GlyPhe: 3.242 ± 0.082
7.25GlyGly: 7.25 ± 0.536
1.958GlyHis: 1.958 ± 0.063
4.759GlyIle: 4.759 ± 0.093
2.506GlyLys: 2.506 ± 0.073
8.982GlyLeu: 8.982 ± 0.148
2.159GlyMet: 2.159 ± 0.075
2.582GlyAsn: 2.582 ± 0.177
3.038GlyPro: 3.038 ± 0.09
3.623GlyGln: 3.623 ± 0.08
5.199GlyArg: 5.199 ± 0.11
4.739GlySer: 4.739 ± 0.235
4.421GlyThr: 4.421 ± 0.249
6.603GlyVal: 6.603 ± 0.111
1.236GlyTrp: 1.236 ± 0.048
2.394GlyTyr: 2.394 ± 0.07
0.0GlyXaa: 0.0 ± 0.0
His
2.344HisAla: 2.344 ± 0.076
0.305HisCys: 0.305 ± 0.022
1.212HisAsp: 1.212 ± 0.049
1.127HisGlu: 1.127 ± 0.049
0.855HisPhe: 0.855 ± 0.039
1.826HisGly: 1.826 ± 0.069
0.674HisHis: 0.674 ± 0.038
1.144HisIle: 1.144 ± 0.047
0.49HisLys: 0.49 ± 0.032
2.311HisLeu: 2.311 ± 0.077
0.42HisMet: 0.42 ± 0.027
0.628HisAsn: 0.628 ± 0.032
1.436HisPro: 1.436 ± 0.057
0.989HisGln: 0.989 ± 0.041
1.565HisArg: 1.565 ± 0.05
1.054HisSer: 1.054 ± 0.039
1.033HisThr: 1.033 ± 0.043
1.398HisVal: 1.398 ± 0.047
0.436HisTrp: 0.436 ± 0.027
0.669HisTyr: 0.669 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
6.687IleAla: 6.687 ± 0.103
0.464IleCys: 0.464 ± 0.031
4.011IleAsp: 4.011 ± 0.11
4.168IleGlu: 4.168 ± 0.084
1.714IlePhe: 1.714 ± 0.061
4.993IleGly: 4.993 ± 0.105
1.093IleHis: 1.093 ± 0.042
2.599IleIle: 2.599 ± 0.07
1.608IleLys: 1.608 ± 0.049
4.344IleLeu: 4.344 ± 0.091
0.959IleMet: 0.959 ± 0.044
2.054IleAsn: 2.054 ± 0.084
2.409IlePro: 2.409 ± 0.091
1.836IleGln: 1.836 ± 0.045
3.334IleArg: 3.334 ± 0.074
3.169IleSer: 3.169 ± 0.076
3.575IleThr: 3.575 ± 0.199
3.587IleVal: 3.587 ± 0.094
0.608IleTrp: 0.608 ± 0.033
1.257IleTyr: 1.257 ± 0.046
0.0IleXaa: 0.0 ± 0.0
Lys
3.477LysAla: 3.477 ± 0.077
0.15LysCys: 0.15 ± 0.017
1.448LysAsp: 1.448 ± 0.054
1.422LysGlu: 1.422 ± 0.062
0.595LysPhe: 0.595 ± 0.031
1.988LysGly: 1.988 ± 0.06
0.559LysHis: 0.559 ± 0.03
1.297LysIle: 1.297 ± 0.046
0.982LysLys: 0.982 ± 0.05
2.531LysLeu: 2.531 ± 0.075
0.556LysMet: 0.556 ± 0.035
0.783LysAsn: 0.783 ± 0.035
1.627LysPro: 1.627 ± 0.064
1.246LysGln: 1.246 ± 0.051
2.078LysArg: 2.078 ± 0.068
1.517LysSer: 1.517 ± 0.046
1.733LysThr: 1.733 ± 0.054
1.8LysVal: 1.8 ± 0.062
0.241LysTrp: 0.241 ± 0.019
0.474LysTyr: 0.474 ± 0.03
0.0LysXaa: 0.0 ± 0.0
Leu
12.882LeuAla: 12.882 ± 0.221
0.887LeuCys: 0.887 ± 0.041
6.132LeuAsp: 6.132 ± 0.125
6.579LeuGlu: 6.579 ± 0.117
3.534LeuPhe: 3.534 ± 0.11
8.679LeuGly: 8.679 ± 0.126
2.132LeuHis: 2.132 ± 0.07
5.899LeuIle: 5.899 ± 0.133
3.084LeuLys: 3.084 ± 0.079
11.206LeuLeu: 11.206 ± 0.262
2.486LeuMet: 2.486 ± 0.09
3.367LeuAsn: 3.367 ± 0.106
5.747LeuPro: 5.747 ± 0.162
4.164LeuGln: 4.164 ± 0.085
7.422LeuArg: 7.422 ± 0.145
6.864LeuSer: 6.864 ± 0.143
6.345LeuThr: 6.345 ± 0.209
7.274LeuVal: 7.274 ± 0.128
1.14LeuTrp: 1.14 ± 0.045
2.125LeuTyr: 2.125 ± 0.074
0.0LeuXaa: 0.0 ± 0.0
Met
2.904MetAla: 2.904 ± 0.081
0.138MetCys: 0.138 ± 0.014
1.149MetAsp: 1.149 ± 0.05
1.156MetGlu: 1.156 ± 0.045
0.564MetPhe: 0.564 ± 0.041
1.767MetGly: 1.767 ± 0.064
0.444MetHis: 0.444 ± 0.028
1.157MetIle: 1.157 ± 0.055
0.751MetLys: 0.751 ± 0.038
2.332MetLeu: 2.332 ± 0.092
0.546MetMet: 0.546 ± 0.035
0.793MetAsn: 0.793 ± 0.038
1.397MetPro: 1.397 ± 0.054
1.064MetGln: 1.064 ± 0.05
1.566MetArg: 1.566 ± 0.063
1.496MetSer: 1.496 ± 0.052
1.522MetThr: 1.522 ± 0.052
1.607MetVal: 1.607 ± 0.057
0.172MetTrp: 0.172 ± 0.017
0.298MetTyr: 0.298 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
3.767AsnAla: 3.767 ± 0.156
0.253AsnCys: 0.253 ± 0.023
1.596AsnAsp: 1.596 ± 0.085
1.664AsnGlu: 1.664 ± 0.05
0.855AsnPhe: 0.855 ± 0.039
2.581AsnGly: 2.581 ± 0.134
0.601AsnHis: 0.601 ± 0.032
1.706AsnIle: 1.706 ± 0.086
0.728AsnLys: 0.728 ± 0.038
3.036AsnLeu: 3.036 ± 0.094
0.562AsnMet: 0.562 ± 0.032
1.046AsnAsn: 1.046 ± 0.068
2.047AsnPro: 2.047 ± 0.056
1.377AsnGln: 1.377 ± 0.043
2.138AsnArg: 2.138 ± 0.048
1.51AsnSer: 1.51 ± 0.098
1.803AsnThr: 1.803 ± 0.117
1.97AsnVal: 1.97 ± 0.092
0.442AsnTrp: 0.442 ± 0.027
0.711AsnTyr: 0.711 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
5.507ProAla: 5.507 ± 0.124
0.333ProCys: 0.333 ± 0.022
3.168ProAsp: 3.168 ± 0.082
3.725ProGlu: 3.725 ± 0.101
1.685ProPhe: 1.685 ± 0.061
3.78ProGly: 3.78 ± 0.086
1.026ProHis: 1.026 ± 0.046
2.482ProIle: 2.482 ± 0.077
1.236ProLys: 1.236 ± 0.05
5.017ProLeu: 5.017 ± 0.138
1.219ProMet: 1.219 ± 0.055
1.535ProAsn: 1.535 ± 0.052
2.26ProPro: 2.26 ± 0.081
1.683ProGln: 1.683 ± 0.06
2.528ProArg: 2.528 ± 0.094
2.61ProSer: 2.61 ± 0.066
2.451ProThr: 2.451 ± 0.068
3.886ProVal: 3.886 ± 0.103
0.686ProTrp: 0.686 ± 0.035
1.062ProTyr: 1.062 ± 0.048
0.0ProXaa: 0.0 ± 0.0
Gln
5.597GlnAla: 5.597 ± 0.111
0.289GlnCys: 0.289 ± 0.022
1.737GlnAsp: 1.737 ± 0.051
1.812GlnGlu: 1.812 ± 0.063
1.452GlnPhe: 1.452 ± 0.049
3.034GlnGly: 3.034 ± 0.074
0.983GlnHis: 0.983 ± 0.045
2.352GlnIle: 2.352 ± 0.059
1.082GlnLys: 1.082 ± 0.047
4.583GlnLeu: 4.583 ± 0.107
0.975GlnMet: 0.975 ± 0.039
1.043GlnAsn: 1.043 ± 0.045
2.265GlnPro: 2.265 ± 0.071
2.193GlnGln: 2.193 ± 0.074
3.523GlnArg: 3.523 ± 0.104
2.386GlnSer: 2.386 ± 0.074
2.351GlnThr: 2.351 ± 0.061
3.014GlnVal: 3.014 ± 0.061
0.742GlnTrp: 0.742 ± 0.04
0.866GlnTyr: 0.866 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
6.62ArgAla: 6.62 ± 0.125
0.617ArgCys: 0.617 ± 0.033
3.532ArgAsp: 3.532 ± 0.081
4.21ArgGlu: 4.21 ± 0.118
2.801ArgPhe: 2.801 ± 0.074
4.55ArgGly: 4.55 ± 0.094
1.675ArgHis: 1.675 ± 0.061
3.858ArgIle: 3.858 ± 0.089
1.728ArgLys: 1.728 ± 0.072
8.26ArgLeu: 8.26 ± 0.174
1.658ArgMet: 1.658 ± 0.065
1.937ArgAsn: 1.937 ± 0.052
2.878ArgPro: 2.878 ± 0.087
3.086ArgGln: 3.086 ± 0.093
4.927ArgArg: 4.927 ± 0.151
3.323ArgSer: 3.323 ± 0.072
2.694ArgThr: 2.694 ± 0.067
4.933ArgVal: 4.933 ± 0.098
1.088ArgTrp: 1.088 ± 0.05
2.203ArgTyr: 2.203 ± 0.065
0.0ArgXaa: 0.0 ± 0.0
Ser
6.533SerAla: 6.533 ± 0.141
0.375SerCys: 0.375 ± 0.029
3.248SerAsp: 3.248 ± 0.118
3.28SerGlu: 3.28 ± 0.077
1.91SerPhe: 1.91 ± 0.059
5.688SerGly: 5.688 ± 0.274
1.225SerHis: 1.225 ± 0.045
2.918SerIle: 2.918 ± 0.095
1.284SerLys: 1.284 ± 0.049
6.012SerLeu: 6.012 ± 0.124
1.232SerMet: 1.232 ± 0.047
1.625SerAsn: 1.625 ± 0.083
2.469SerPro: 2.469 ± 0.075
2.163SerGln: 2.163 ± 0.057
3.472SerArg: 3.472 ± 0.077
3.084SerSer: 3.084 ± 0.118
2.785SerThr: 2.785 ± 0.125
4.207SerVal: 4.207 ± 0.126
0.741SerTrp: 0.741 ± 0.032
1.154SerTyr: 1.154 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
6.052ThrAla: 6.052 ± 0.209
0.401ThrCys: 0.401 ± 0.028
3.139ThrAsp: 3.139 ± 0.121
2.964ThrGlu: 2.964 ± 0.065
1.611ThrPhe: 1.611 ± 0.071
5.357ThrGly: 5.357 ± 0.261
1.239ThrHis: 1.239 ± 0.043
2.753ThrIle: 2.753 ± 0.15
1.029ThrLys: 1.029 ± 0.044
6.9ThrLeu: 6.9 ± 0.26
0.986ThrMet: 0.986 ± 0.044
1.472ThrAsn: 1.472 ± 0.107
3.249ThrPro: 3.249 ± 0.08
2.108ThrGln: 2.108 ± 0.072
3.142ThrArg: 3.142 ± 0.076
2.488ThrSer: 2.488 ± 0.126
3.022ThrThr: 3.022 ± 0.184
4.213ThrVal: 4.213 ± 0.16
0.58ThrTrp: 0.58 ± 0.028
1.064ThrTyr: 1.064 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
7.973ValAla: 7.973 ± 0.121
0.681ValCys: 0.681 ± 0.035
4.549ValAsp: 4.549 ± 0.109
4.643ValGlu: 4.643 ± 0.08
2.703ValPhe: 2.703 ± 0.073
5.705ValGly: 5.705 ± 0.116
1.463ValHis: 1.463 ± 0.059
4.574ValIle: 4.574 ± 0.099
1.933ValLys: 1.933 ± 0.056
7.642ValLeu: 7.642 ± 0.127
1.895ValMet: 1.895 ± 0.066
2.414ValAsn: 2.414 ± 0.093
3.039ValPro: 3.039 ± 0.086
2.273ValGln: 2.273 ± 0.054
4.363ValArg: 4.363 ± 0.09
4.508ValSer: 4.508 ± 0.11
4.27ValThr: 4.27 ± 0.167
6.018ValVal: 6.018 ± 0.113
0.841ValTrp: 0.841 ± 0.034
1.642ValTyr: 1.642 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
1.164TrpAla: 1.164 ± 0.044
0.15TrpCys: 0.15 ± 0.016
0.576TrpAsp: 0.576 ± 0.031
0.634TrpGlu: 0.634 ± 0.029
0.532TrpPhe: 0.532 ± 0.03
0.835TrpGly: 0.835 ± 0.04
0.365TrpHis: 0.365 ± 0.025
0.78TrpIle: 0.78 ± 0.035
0.278TrpLys: 0.278 ± 0.022
2.06TrpLeu: 2.06 ± 0.077
0.35TrpMet: 0.35 ± 0.023
0.378TrpAsn: 0.378 ± 0.028
0.634TrpPro: 0.634 ± 0.037
0.913TrpGln: 0.913 ± 0.033
1.06TrpArg: 1.06 ± 0.046
0.715TrpSer: 0.715 ± 0.033
0.583TrpThr: 0.583 ± 0.03
1.091TrpVal: 1.091 ± 0.051
0.23TrpTrp: 0.23 ± 0.023
0.299TrpTyr: 0.299 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.502TyrAla: 2.502 ± 0.063
0.261TyrCys: 0.261 ± 0.02
1.254TyrAsp: 1.254 ± 0.048
1.264TyrGlu: 1.264 ± 0.04
0.952TyrPhe: 0.952 ± 0.045
2.155TyrGly: 2.155 ± 0.065
0.54TyrHis: 0.54 ± 0.03
0.967TyrIle: 0.967 ± 0.045
0.488TyrLys: 0.488 ± 0.026
2.702TyrLeu: 2.702 ± 0.075
0.375TyrMet: 0.375 ± 0.025
0.66TyrAsn: 0.66 ± 0.032
1.229TyrPro: 1.229 ± 0.05
1.11TyrGln: 1.11 ± 0.04
1.985TyrArg: 1.985 ± 0.053
1.267TyrSer: 1.267 ± 0.039
1.22TyrThr: 1.22 ± 0.056
1.463TyrVal: 1.463 ± 0.053
0.411TyrTrp: 0.411 ± 0.025
0.636TyrTyr: 0.636 ± 0.032
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2078 proteins (708737 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski