Calothrix sp. HK-06

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Calotrichaceae; Calothrix; unclassified Calothrix

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8121 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q4RA92|A0A1Q4RA92_9CYAN Uncharacterized protein OS=Calothrix sp. HK-06 OX=1137096 GN=NIES2101_42785 PE=4 SV=1
MM1 pKa = 7.83VGDD4 pKa = 4.66FFRR7 pKa = 11.84KK8 pKa = 10.06AKK10 pKa = 10.61DD11 pKa = 3.53FLASGVDD18 pKa = 3.29SDD20 pKa = 3.98RR21 pKa = 11.84DD22 pKa = 3.38EE23 pKa = 3.99EE24 pKa = 4.4RR25 pKa = 11.84EE26 pKa = 3.82YY27 pKa = 11.63GEE29 pKa = 4.11NVRR32 pKa = 11.84PASEE36 pKa = 4.75DD37 pKa = 3.75PYY39 pKa = 11.39GDD41 pKa = 4.05PADD44 pKa = 3.44QQYY47 pKa = 9.42YY48 pKa = 9.86GEE50 pKa = 4.2RR51 pKa = 11.84AGYY54 pKa = 7.77EE55 pKa = 3.75QYY57 pKa = 11.74GDD59 pKa = 3.56VRR61 pKa = 11.84PASEE65 pKa = 5.18DD66 pKa = 3.79PYY68 pKa = 11.41GDD70 pKa = 3.93PADD73 pKa = 3.76QYY75 pKa = 11.75GSAGYY80 pKa = 9.05GQYY83 pKa = 11.35GDD85 pKa = 4.07VRR87 pKa = 11.84PASEE91 pKa = 5.18DD92 pKa = 3.79PYY94 pKa = 11.41GDD96 pKa = 4.17PADD99 pKa = 3.24QYY101 pKa = 11.05GNVRR105 pKa = 11.84RR106 pKa = 11.84ASEE109 pKa = 4.28DD110 pKa = 3.67PYY112 pKa = 11.5GDD114 pKa = 3.81PADD117 pKa = 4.17DD118 pKa = 3.27EE119 pKa = 5.27GYY121 pKa = 10.65RR122 pKa = 3.69

Molecular weight: 13.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q4RSW7|A0A1Q4RSW7_9CYAN D-alanyl-D-alanine carboxypeptidase OS=Calothrix sp. HK-06 OX=1137096 GN=NIES2101_16810 PE=3 SV=1
MM1 pKa = 7.4KK2 pKa = 9.16RR3 pKa = 11.84TLEE6 pKa = 3.83GTNRR10 pKa = 11.84KK11 pKa = 9.13RR12 pKa = 11.84KK13 pKa = 7.54RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPDD26 pKa = 3.17GRR28 pKa = 11.84NVIKK32 pKa = 10.5ARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.92GRR39 pKa = 11.84HH40 pKa = 4.95RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8121

0

8121

2607155

27

8011

321.0

35.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.901 ± 0.028

0.999 ± 0.01

4.809 ± 0.023

6.099 ± 0.028

4.046 ± 0.019

6.505 ± 0.039

1.764 ± 0.013

7.155 ± 0.022

5.318 ± 0.026

10.617 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.834 ± 0.014

5.038 ± 0.026

4.364 ± 0.021

5.037 ± 0.023

4.823 ± 0.021

6.636 ± 0.022

5.849 ± 0.022

6.656 ± 0.023

1.347 ± 0.012

3.2 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski