Amino acid dipepetide frequency for Calothrix sp. HK-06

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.756AlaAla: 6.756 ± 0.072	0.855AlaCys: 0.855 ± 0.02	3.538AlaAsp: 3.538 ± 0.04	4.545AlaGlu: 4.545 ± 0.044	3.07AlaPhe: 3.07 ± 0.035	5.152AlaGly: 5.152 ± 0.063	1.24AlaHis: 1.24 ± 0.024	6.175AlaIle: 6.175 ± 0.059	4.116AlaLys: 4.116 ± 0.041	8.409AlaLeu: 8.409 ± 0.058	1.581AlaMet: 1.581 ± 0.031	3.746AlaAsn: 3.746 ± 0.052	3.281AlaPro: 3.281 ± 0.046	3.768AlaGln: 3.768 ± 0.046	3.704AlaArg: 3.704 ± 0.038	5.428AlaSer: 5.428 ± 0.054	4.681AlaThr: 4.681 ± 0.053	5.577AlaVal: 5.577 ± 0.051	0.998AlaTrp: 0.998 ± 0.023	2.384AlaTyr: 2.384 ± 0.033	0.001AlaXaa: 0.001 ± 0.001
Cys	0.641CysAla: 0.641 ± 0.018	0.161CysCys: 0.161 ± 0.008	0.524CysAsp: 0.524 ± 0.014	0.544CysGlu: 0.544 ± 0.016	0.434CysPhe: 0.434 ± 0.014	0.771CysGly: 0.771 ± 0.019	0.255CysHis: 0.255 ± 0.01	0.674CysIle: 0.674 ± 0.017	0.434CysLys: 0.434 ± 0.013	1.093CysLeu: 1.093 ± 0.023	0.181CysMet: 0.181 ± 0.008	0.464CysAsn: 0.464 ± 0.015	0.502CysPro: 0.502 ± 0.015	0.512CysGln: 0.512 ± 0.015	0.499CysArg: 0.499 ± 0.014	0.638CysSer: 0.638 ± 0.016	0.51CysThr: 0.51 ± 0.013	0.618CysVal: 0.618 ± 0.017	0.142CysTrp: 0.142 ± 0.008	0.392CysTyr: 0.392 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.725AspAla: 3.725 ± 0.049	0.513AspCys: 0.513 ± 0.014	2.289AspAsp: 2.289 ± 0.033	2.962AspGlu: 2.962 ± 0.039	2.393AspPhe: 2.393 ± 0.032	3.364AspGly: 3.364 ± 0.055	0.519AspHis: 0.519 ± 0.016	4.103AspIle: 4.103 ± 0.047	2.931AspLys: 2.931 ± 0.043	5.019AspLeu: 5.019 ± 0.05	0.795AspMet: 0.795 ± 0.018	2.534AspAsn: 2.534 ± 0.039	1.832AspPro: 1.832 ± 0.028	1.271AspGln: 1.271 ± 0.021	2.065AspArg: 2.065 ± 0.032	3.07AspSer: 3.07 ± 0.035	2.848AspThr: 2.848 ± 0.031	3.143AspVal: 3.143 ± 0.035	0.835AspTrp: 0.835 ± 0.022	1.883AspTyr: 1.883 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	5.236GluAla: 5.236 ± 0.058	0.527GluCys: 0.527 ± 0.014	2.58GluAsp: 2.58 ± 0.034	3.852GluGlu: 3.852 ± 0.05	2.459GluPhe: 2.459 ± 0.033	3.055GluGly: 3.055 ± 0.036	1.061GluHis: 1.061 ± 0.021	4.736GluIle: 4.736 ± 0.053	3.672GluLys: 3.672 ± 0.044	6.862GluLeu: 6.862 ± 0.062	1.214GluMet: 1.214 ± 0.024	2.937GluAsn: 2.937 ± 0.033	2.25GluPro: 2.25 ± 0.031	3.362GluGln: 3.362 ± 0.041	3.384GluArg: 3.384 ± 0.039	3.359GluSer: 3.359 ± 0.034	3.371GluThr: 3.371 ± 0.044	4.295GluVal: 4.295 ± 0.048	0.761GluTrp: 0.761 ± 0.019	2.016GluTyr: 2.016 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	3.193PheAla: 3.193 ± 0.035	0.529PheCys: 0.529 ± 0.014	2.406PheAsp: 2.406 ± 0.035	2.307PheGlu: 2.307 ± 0.03	1.687PhePhe: 1.687 ± 0.03	3.044PheGly: 3.044 ± 0.039	0.696PheHis: 0.696 ± 0.017	2.771PheIle: 2.771 ± 0.037	1.874PheLys: 1.874 ± 0.029	3.942PheLeu: 3.942 ± 0.042	0.702PheMet: 0.702 ± 0.017	2.09PheAsn: 2.09 ± 0.033	1.639PhePro: 1.639 ± 0.025	1.65PheGln: 1.65 ± 0.027	1.718PheArg: 1.718 ± 0.025	3.021PheSer: 3.021 ± 0.042	2.553PheThr: 2.553 ± 0.04	2.577PheVal: 2.577 ± 0.035	0.64PheTrp: 0.64 ± 0.015	1.425PheTyr: 1.425 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	5.601GlyAla: 5.601 ± 0.067	0.753GlyCys: 0.753 ± 0.018	3.103GlyAsp: 3.103 ± 0.05	3.647GlyGlu: 3.647 ± 0.036	2.961GlyPhe: 2.961 ± 0.038	4.605GlyGly: 4.605 ± 0.072	1.155GlyHis: 1.155 ± 0.027	5.067GlyIle: 5.067 ± 0.049	4.212GlyLys: 4.212 ± 0.041	6.482GlyLeu: 6.482 ± 0.051	1.439GlyMet: 1.439 ± 0.026	3.403GlyAsn: 3.403 ± 0.072	1.01GlyPro: 1.01 ± 0.02	2.655GlyGln: 2.655 ± 0.042	3.083GlyArg: 3.083 ± 0.041	4.036GlySer: 4.036 ± 0.054	3.87GlyThr: 3.87 ± 0.052	4.537GlyVal: 4.537 ± 0.051	1.006GlyTrp: 1.006 ± 0.022	2.429GlyTyr: 2.429 ± 0.035	0.0GlyXaa: 0.0 ± 0.0
His	1.068HisAla: 1.068 ± 0.021	0.229HisCys: 0.229 ± 0.011	0.82HisAsp: 0.82 ± 0.019	1.017HisGlu: 1.017 ± 0.022	0.756HisPhe: 0.756 ± 0.018	1.081HisGly: 1.081 ± 0.023	0.504HisHis: 0.504 ± 0.014	1.187HisIle: 1.187 ± 0.021	0.851HisLys: 0.851 ± 0.019	2.005HisLeu: 2.005 ± 0.028	0.272HisMet: 0.272 ± 0.011	0.834HisAsn: 0.834 ± 0.017	1.151HisPro: 1.151 ± 0.021	0.929HisGln: 0.929 ± 0.02	0.96HisArg: 0.96 ± 0.022	1.173HisSer: 1.173 ± 0.023	0.918HisThr: 0.918 ± 0.018	0.896HisVal: 0.896 ± 0.019	0.312HisTrp: 0.312 ± 0.011	0.68HisTyr: 0.68 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	6.425IleAla: 6.425 ± 0.051	0.771IleCys: 0.771 ± 0.018	3.955IleAsp: 3.955 ± 0.043	4.664IleGlu: 4.664 ± 0.046	2.801IlePhe: 2.801 ± 0.037	4.645IleGly: 4.645 ± 0.044	1.241IleHis: 1.241 ± 0.02	4.72IleIle: 4.72 ± 0.054	3.787IleLys: 3.787 ± 0.041	6.948IleLeu: 6.948 ± 0.056	1.006IleMet: 1.006 ± 0.022	3.895IleAsn: 3.895 ± 0.05	3.545IlePro: 3.545 ± 0.041	3.13IleGln: 3.13 ± 0.042	3.158IleArg: 3.158 ± 0.036	4.956IleSer: 4.956 ± 0.051	4.353IleThr: 4.353 ± 0.048	4.441IleVal: 4.441 ± 0.04	0.877IleTrp: 0.877 ± 0.021	2.231IleTyr: 2.231 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.169LysAla: 4.169 ± 0.043	0.407LysCys: 0.407 ± 0.014	2.462LysAsp: 2.462 ± 0.038	3.047LysGlu: 3.047 ± 0.042	2.1LysPhe: 2.1 ± 0.031	2.862LysGly: 2.862 ± 0.039	0.98LysHis: 0.98 ± 0.023	4.027LysIle: 4.027 ± 0.043	2.625LysLys: 2.625 ± 0.039	6.167LysLeu: 6.167 ± 0.055	1.037LysMet: 1.037 ± 0.022	2.626LysAsn: 2.626 ± 0.039	2.639LysPro: 2.639 ± 0.031	3.137LysGln: 3.137 ± 0.043	2.514LysArg: 2.514 ± 0.032	3.399LysSer: 3.399 ± 0.037	3.085LysThr: 3.085 ± 0.039	3.489LysVal: 3.489 ± 0.038	0.58LysTrp: 0.58 ± 0.018	1.83LysTyr: 1.83 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	8.727LeuAla: 8.727 ± 0.071	1.015LeuCys: 1.015 ± 0.02	5.161LeuAsp: 5.161 ± 0.049	7.019LeuGlu: 7.019 ± 0.06	3.877LeuPhe: 3.877 ± 0.049	7.049LeuGly: 7.049 ± 0.058	1.882LeuHis: 1.882 ± 0.03	6.898LeuIle: 6.898 ± 0.068	5.77LeuLys: 5.77 ± 0.058	10.917LeuLeu: 10.917 ± 0.087	2.077LeuMet: 2.077 ± 0.027	5.078LeuAsn: 5.078 ± 0.05	5.318LeuPro: 5.318 ± 0.052	5.474LeuGln: 5.474 ± 0.057	5.243LeuArg: 5.243 ± 0.049	7.436LeuSer: 7.436 ± 0.069	6.134LeuThr: 6.134 ± 0.05	6.945LeuVal: 6.945 ± 0.052	1.339LeuTrp: 1.339 ± 0.035	2.809LeuTyr: 2.809 ± 0.034	0.0LeuXaa: 0.0 ± 0.0
Met	1.569MetAla: 1.569 ± 0.031	0.145MetCys: 0.145 ± 0.008	0.708MetAsp: 0.708 ± 0.017	0.934MetGlu: 0.934 ± 0.02	0.647MetPhe: 0.647 ± 0.019	1.227MetGly: 1.227 ± 0.029	0.339MetHis: 0.339 ± 0.013	1.051MetIle: 1.051 ± 0.022	1.01MetLys: 1.01 ± 0.022	2.051MetLeu: 2.051 ± 0.029	0.457MetMet: 0.457 ± 0.013	0.905MetAsn: 0.905 ± 0.02	0.906MetPro: 0.906 ± 0.017	0.953MetGln: 0.953 ± 0.021	0.987MetArg: 0.987 ± 0.02	1.385MetSer: 1.385 ± 0.024	1.211MetThr: 1.211 ± 0.023	1.226MetVal: 1.226 ± 0.023	0.204MetTrp: 0.204 ± 0.01	0.429MetTyr: 0.429 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.545AsnAla: 3.545 ± 0.048	0.522AsnCys: 0.522 ± 0.013	2.067AsnAsp: 2.067 ± 0.039	2.301AsnGlu: 2.301 ± 0.035	2.155AsnPhe: 2.155 ± 0.031	3.192AsnGly: 3.192 ± 0.052	0.895AsnHis: 0.895 ± 0.021	3.9AsnIle: 3.9 ± 0.051	2.445AsnLys: 2.445 ± 0.035	5.781AsnLeu: 5.781 ± 0.062	0.74AsnMet: 0.74 ± 0.017	2.882AsnAsn: 2.882 ± 0.046	2.882AsnPro: 2.882 ± 0.036	2.917AsnGln: 2.917 ± 0.039	2.46AsnArg: 2.46 ± 0.031	3.511AsnSer: 3.511 ± 0.039	2.852AsnThr: 2.852 ± 0.042	2.748AsnVal: 2.748 ± 0.038	0.775AsnTrp: 0.775 ± 0.017	1.806AsnTyr: 1.806 ± 0.025	0.001AsnXaa: 0.001 ± 0.001
Pro	2.859ProAla: 2.859 ± 0.034	0.335ProCys: 0.335 ± 0.013	2.687ProAsp: 2.687 ± 0.037	3.39ProGlu: 3.39 ± 0.04	1.678ProPhe: 1.678 ± 0.028	2.794ProGly: 2.794 ± 0.038	0.785ProHis: 0.785 ± 0.018	3.064ProIle: 3.064 ± 0.033	2.244ProLys: 2.244 ± 0.03	4.24ProLeu: 4.24 ± 0.046	0.743ProMet: 0.743 ± 0.017	2.368ProAsn: 2.368 ± 0.031	1.874ProPro: 1.874 ± 0.04	2.245ProGln: 2.245 ± 0.038	1.57ProArg: 1.57 ± 0.027	2.945ProSer: 2.945 ± 0.041	2.797ProThr: 2.797 ± 0.044	3.238ProVal: 3.238 ± 0.039	0.511ProTrp: 0.511 ± 0.014	1.27ProTyr: 1.27 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	4.333GlnAla: 4.333 ± 0.057	0.331GlnCys: 0.331 ± 0.012	2.032GlnAsp: 2.032 ± 0.032	3.164GlnGlu: 3.164 ± 0.038	1.665GlnPhe: 1.665 ± 0.028	2.835GlnGly: 2.835 ± 0.034	0.902GlnHis: 0.902 ± 0.019	3.658GlnIle: 3.658 ± 0.042	2.842GlnLys: 2.842 ± 0.035	5.55GlnLeu: 5.55 ± 0.061	0.981GlnMet: 0.981 ± 0.015	2.355GlnAsn: 2.355 ± 0.031	2.364GlnPro: 2.364 ± 0.035	3.443GlnGln: 3.443 ± 0.052	2.639GlnArg: 2.639 ± 0.032	2.899GlnSer: 2.899 ± 0.036	2.894GlnThr: 2.894 ± 0.039	3.572GlnVal: 3.572 ± 0.047	0.591GlnTrp: 0.591 ± 0.016	1.323GlnTyr: 1.323 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	3.354ArgAla: 3.354 ± 0.039	0.466ArgCys: 0.466 ± 0.014	2.38ArgAsp: 2.38 ± 0.035	3.126ArgGlu: 3.126 ± 0.04	2.062ArgPhe: 2.062 ± 0.029	2.762ArgGly: 2.762 ± 0.037	0.934ArgHis: 0.934 ± 0.017	3.274ArgIle: 3.274 ± 0.04	2.31ArgLys: 2.31 ± 0.031	5.452ArgLeu: 5.452 ± 0.057	0.962ArgMet: 0.962 ± 0.019	2.313ArgAsn: 2.313 ± 0.033	1.847ArgPro: 1.847 ± 0.031	2.775ArgGln: 2.775 ± 0.039	2.865ArgArg: 2.865 ± 0.04	2.877ArgSer: 2.877 ± 0.043	2.541ArgThr: 2.541 ± 0.032	3.403ArgVal: 3.403 ± 0.04	0.71ArgTrp: 0.71 ± 0.019	1.82ArgTyr: 1.82 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	4.42SerAla: 4.42 ± 0.047	0.624SerCys: 0.624 ± 0.016	3.224SerAsp: 3.224 ± 0.04	4.039SerGlu: 4.039 ± 0.042	2.741SerPhe: 2.741 ± 0.032	4.816SerGly: 4.816 ± 0.053	1.224SerHis: 1.224 ± 0.024	4.495SerIle: 4.495 ± 0.039	3.394SerLys: 3.394 ± 0.041	7.234SerLeu: 7.234 ± 0.065	1.199SerMet: 1.199 ± 0.025	3.592SerAsn: 3.592 ± 0.042	2.864SerPro: 2.864 ± 0.038	3.509SerGln: 3.509 ± 0.042	3.043SerArg: 3.043 ± 0.041	4.785SerSer: 4.785 ± 0.056	3.971SerThr: 3.971 ± 0.045	4.304SerVal: 4.304 ± 0.043	0.867SerTrp: 0.867 ± 0.015	2.016SerTyr: 2.016 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	4.708ThrAla: 4.708 ± 0.05	0.523ThrCys: 0.523 ± 0.015	2.66ThrAsp: 2.66 ± 0.032	3.308ThrGlu: 3.308 ± 0.035	2.299ThrPhe: 2.299 ± 0.032	4.394ThrGly: 4.394 ± 0.057	1.017ThrHis: 1.017 ± 0.019	4.132ThrIle: 4.132 ± 0.04	2.73ThrLys: 2.73 ± 0.032	6.183ThrLeu: 6.183 ± 0.057	0.825ThrMet: 0.825 ± 0.018	2.809ThrAsn: 2.809 ± 0.045	3.299ThrPro: 3.299 ± 0.049	2.986ThrGln: 2.986 ± 0.043	2.422ThrArg: 2.422 ± 0.029	3.994ThrSer: 3.994 ± 0.047	3.593ThrThr: 3.593 ± 0.052	4.215ThrVal: 4.215 ± 0.042	0.724ThrTrp: 0.724 ± 0.018	1.67ThrTyr: 1.67 ± 0.029	0.001ThrXaa: 0.001 ± 0.001
Val	5.698ValAla: 5.698 ± 0.051	0.697ValCys: 0.697 ± 0.017	3.335ValAsp: 3.335 ± 0.037	4.454ValGlu: 4.454 ± 0.042	2.619ValPhe: 2.619 ± 0.031	4.437ValGly: 4.437 ± 0.047	1.01ValHis: 1.01 ± 0.02	4.738ValIle: 4.738 ± 0.038	3.661ValLys: 3.661 ± 0.039	6.583ValLeu: 6.583 ± 0.056	1.345ValMet: 1.345 ± 0.027	3.309ValAsn: 3.309 ± 0.042	2.758ValPro: 2.758 ± 0.035	2.762ValGln: 2.762 ± 0.035	3.149ValArg: 3.149 ± 0.04	4.459ValSer: 4.459 ± 0.048	4.029ValThr: 4.029 ± 0.038	4.736ValVal: 4.736 ± 0.047	0.835ValTrp: 0.835 ± 0.021	1.948ValTyr: 1.948 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	0.828TrpAla: 0.828 ± 0.02	0.152TrpCys: 0.152 ± 0.008	0.664TrpAsp: 0.664 ± 0.018	0.839TrpGlu: 0.839 ± 0.017	0.608TrpPhe: 0.608 ± 0.017	0.893TrpGly: 0.893 ± 0.021	0.348TrpHis: 0.348 ± 0.012	0.796TrpIle: 0.796 ± 0.021	0.663TrpLys: 0.663 ± 0.017	1.725TrpLeu: 1.725 ± 0.028	0.305TrpMet: 0.305 ± 0.012	0.677TrpAsn: 0.677 ± 0.017	0.134TrpPro: 0.134 ± 0.007	1.003TrpGln: 1.003 ± 0.021	0.876TrpArg: 0.876 ± 0.022	0.838TrpSer: 0.838 ± 0.019	0.555TrpThr: 0.555 ± 0.014	0.909TrpVal: 0.909 ± 0.021	0.229TrpTrp: 0.229 ± 0.009	0.43TrpTyr: 0.43 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.151TyrAla: 2.151 ± 0.028	0.434TyrCys: 0.434 ± 0.013	1.499TyrAsp: 1.499 ± 0.03	1.83TyrGlu: 1.83 ± 0.029	1.45TyrPhe: 1.45 ± 0.03	2.075TyrGly: 2.075 ± 0.028	0.658TyrHis: 0.658 ± 0.017	2.084TyrIle: 2.084 ± 0.029	1.611TyrLys: 1.611 ± 0.025	3.523TyrLeu: 3.523 ± 0.039	0.483TyrMet: 0.483 ± 0.012	1.559TyrAsn: 1.559 ± 0.031	1.542TyrPro: 1.542 ± 0.025	1.895TyrGln: 1.895 ± 0.029	1.892TyrArg: 1.892 ± 0.033	2.151TyrSer: 2.151 ± 0.031	1.724TyrThr: 1.724 ± 0.027	1.694TyrVal: 1.694 ± 0.027	0.536TyrTrp: 0.536 ± 0.015	1.21TyrTyr: 1.21 ± 0.025	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 8121 proteins (2607155 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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