Staphylococcus phage 812

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Twortvirinae; Kayvirus; unclassified Kayvirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1WEZ8|A0A0U1WEZ8_9CAUD Putative membrane-associated protein OS=Staphylococcus phage 812 OX=307898 GN=812_046 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.26KK3 pKa = 9.73INSVIKK9 pKa = 10.92GEE11 pKa = 4.2GKK13 pKa = 10.02KK14 pKa = 10.08VQTADD19 pKa = 3.12VRR21 pKa = 11.84KK22 pKa = 9.23ISYY25 pKa = 8.66YY26 pKa = 10.15VKK28 pKa = 10.35DD29 pKa = 4.36YY30 pKa = 10.2NPCMTVDD37 pKa = 4.45DD38 pKa = 5.09ANDD41 pKa = 3.4YY42 pKa = 10.76NATSQYY48 pKa = 10.96LVSDD52 pKa = 3.42NGKK55 pKa = 10.45FIAKK59 pKa = 9.62YY60 pKa = 9.38NKK62 pKa = 10.09DD63 pKa = 3.24MNAVGFYY70 pKa = 10.07EE71 pKa = 4.24EE72 pKa = 4.91SGDD75 pKa = 4.03TVKK78 pKa = 10.75HH79 pKa = 5.35LTHH82 pKa = 6.44TTPEE86 pKa = 4.1RR87 pKa = 11.84LEE89 pKa = 4.14GTVFTIEE96 pKa = 4.26EE97 pKa = 4.15EE98 pKa = 4.52TEE100 pKa = 3.37IDD102 pKa = 5.22LINDD106 pKa = 4.19TLPQGDD112 pKa = 4.13ILIKK116 pKa = 10.75FSDD119 pKa = 3.82GSIYY123 pKa = 10.81LPDD126 pKa = 3.99NEE128 pKa = 4.94SVLDD132 pKa = 3.97SVNYY136 pKa = 10.3LADD139 pKa = 4.14NDD141 pKa = 4.11WDD143 pKa = 4.03SVDD146 pKa = 4.77DD147 pKa = 4.66IIYY150 pKa = 9.68TGLSKK155 pKa = 11.1GNSEE159 pKa = 3.98NCIVDD164 pKa = 4.23FNYY167 pKa = 10.51NNYY170 pKa = 10.39DD171 pKa = 2.83IGYY174 pKa = 10.04DD175 pKa = 3.6DD176 pKa = 5.84VEE178 pKa = 6.23DD179 pKa = 5.06EE180 pKa = 4.88DD181 pKa = 5.07VCDD184 pKa = 4.64NYY186 pKa = 10.78PEE188 pKa = 4.77CEE190 pKa = 4.02CSNYY194 pKa = 9.78CSSTGEE200 pKa = 4.12YY201 pKa = 9.67IGNN204 pKa = 3.72

Molecular weight: 23.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1WF52|A0A0U1WF52_9CAUD Uncharacterized protein OS=Staphylococcus phage 812 OX=307898 GN=812_144 PE=4 SV=1
MM1 pKa = 6.69TNKK4 pKa = 10.05RR5 pKa = 11.84KK6 pKa = 8.48TIGKK10 pKa = 9.15ISNSRR15 pKa = 11.84ATWNINPVTKK25 pKa = 10.43VKK27 pKa = 10.38KK28 pKa = 10.53DD29 pKa = 3.09KK30 pKa = 10.55TKK32 pKa = 9.9YY33 pKa = 9.17SRR35 pKa = 11.84KK36 pKa = 9.29NKK38 pKa = 10.13HH39 pKa = 6.39KK40 pKa = 11.03GLDD43 pKa = 3.44NYY45 pKa = 11.15NN46 pKa = 3.27

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

221

0

221

42568

15

1352

192.6

22.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.158 ± 0.203

0.524 ± 0.053

6.907 ± 0.122

8.356 ± 0.238

3.97 ± 0.119

5.791 ± 0.316

1.64 ± 0.091

7.59 ± 0.178

9.26 ± 0.255

8.084 ± 0.194

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.092

6.655 ± 0.14

2.734 ± 0.158

3.519 ± 0.164

3.611 ± 0.112

6.568 ± 0.173

5.788 ± 0.181

6.331 ± 0.151

0.85 ± 0.047

5.126 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski