Amino acid dipepetide frequency for Staphylococcus phage 812

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.047AlaAla: 0.047 ± 0.031
0.188AlaCys: 0.188 ± 0.064
2.255AlaAsp: 2.255 ± 0.233
3.078AlaGlu: 3.078 ± 0.33
1.785AlaPhe: 1.785 ± 0.174
2.255AlaGly: 2.255 ± 0.308
0.963AlaHis: 0.963 ± 0.158
3.782AlaIle: 3.782 ± 0.322
3.994AlaLys: 3.994 ± 0.369
3.571AlaLeu: 3.571 ± 0.279
1.222AlaMet: 1.222 ± 0.264
2.373AlaAsn: 2.373 ± 0.27
1.175AlaPro: 1.175 ± 0.193
1.809AlaGln: 1.809 ± 0.256
1.55AlaArg: 1.55 ± 0.19
3.359AlaSer: 3.359 ± 0.371
2.937AlaThr: 2.937 ± 0.261
2.772AlaVal: 2.772 ± 0.263
0.376AlaTrp: 0.376 ± 0.082
2.067AlaTyr: 2.067 ± 0.208
0.023AlaXaa: 0.023 ± 0.021
Cys
0.188CysAla: 0.188 ± 0.076
0.07CysCys: 0.07 ± 0.043
0.282CysAsp: 0.282 ± 0.08
0.352CysGlu: 0.352 ± 0.116
0.235CysPhe: 0.235 ± 0.075
0.376CysGly: 0.376 ± 0.104
0.141CysHis: 0.141 ± 0.059
0.54CysIle: 0.54 ± 0.137
0.493CysLys: 0.493 ± 0.107
0.446CysLeu: 0.446 ± 0.106
0.07CysMet: 0.07 ± 0.039
0.094CysAsn: 0.094 ± 0.05
0.188CysPro: 0.188 ± 0.083
0.211CysGln: 0.211 ± 0.114
0.188CysArg: 0.188 ± 0.07
0.399CysSer: 0.399 ± 0.099
0.329CysThr: 0.329 ± 0.103
0.235CysVal: 0.235 ± 0.07
0.047CysTrp: 0.047 ± 0.035
0.352CysTyr: 0.352 ± 0.098
0.0CysXaa: 0.0 ± 0.0
Asp
2.913AspAla: 2.913 ± 0.276
0.352AspCys: 0.352 ± 0.1
4.229AspAsp: 4.229 ± 0.376
5.074AspGlu: 5.074 ± 0.329
3.383AspPhe: 3.383 ± 0.342
3.359AspGly: 3.359 ± 0.295
0.517AspHis: 0.517 ± 0.114
6.46AspIle: 6.46 ± 0.478
6.813AspLys: 6.813 ± 0.45
5.615AspLeu: 5.615 ± 0.336
2.302AspMet: 2.302 ± 0.256
5.591AspAsn: 5.591 ± 0.358
1.574AspPro: 1.574 ± 0.176
1.128AspGln: 1.128 ± 0.179
2.42AspArg: 2.42 ± 0.262
4.299AspSer: 4.299 ± 0.345
3.876AspThr: 3.876 ± 0.28
4.651AspVal: 4.651 ± 0.428
0.564AspTrp: 0.564 ± 0.123
3.9AspTyr: 3.9 ± 0.324
0.047AspXaa: 0.047 ± 0.035
Glu
3.853GluAla: 3.853 ± 0.298
0.423GluCys: 0.423 ± 0.131
6.742GluAsp: 6.742 ± 0.535
8.81GluGlu: 8.81 ± 0.691
3.148GluPhe: 3.148 ± 0.302
4.299GluGly: 4.299 ± 0.265
1.668GluHis: 1.668 ± 0.2
5.685GluIle: 5.685 ± 0.434
7.541GluLys: 7.541 ± 0.507
7.165GluLeu: 7.165 ± 0.486
2.349GluMet: 2.349 ± 0.258
4.346GluAsn: 4.346 ± 0.315
2.161GluPro: 2.161 ± 0.412
3.9GluGln: 3.9 ± 0.347
3.031GluArg: 3.031 ± 0.268
4.769GluSer: 4.769 ± 0.346
3.782GluThr: 3.782 ± 0.311
4.933GluVal: 4.933 ± 0.399
0.728GluTrp: 0.728 ± 0.126
4.229GluTyr: 4.229 ± 0.366
0.0GluXaa: 0.0 ± 0.0
Phe
1.386PheAla: 1.386 ± 0.181
0.258PheCys: 0.258 ± 0.07
2.584PheAsp: 2.584 ± 0.239
2.655PheGlu: 2.655 ± 0.257
1.41PhePhe: 1.41 ± 0.164
2.138PheGly: 2.138 ± 0.26
0.564PheHis: 0.564 ± 0.116
3.853PheIle: 3.853 ± 0.351
3.712PheLys: 3.712 ± 0.268
3.101PheLeu: 3.101 ± 0.291
0.869PheMet: 0.869 ± 0.146
3.265PheAsn: 3.265 ± 0.252
1.034PhePro: 1.034 ± 0.139
1.386PheGln: 1.386 ± 0.163
1.198PheArg: 1.198 ± 0.152
2.749PheSer: 2.749 ± 0.275
2.302PheThr: 2.302 ± 0.25
2.655PheVal: 2.655 ± 0.285
0.352PheTrp: 0.352 ± 0.117
2.232PheTyr: 2.232 ± 0.251
0.0PheXaa: 0.0 ± 0.0
Gly
2.631GlyAla: 2.631 ± 0.55
0.211GlyCys: 0.211 ± 0.07
3.618GlyAsp: 3.618 ± 0.323
4.487GlyGlu: 4.487 ± 0.325
2.42GlyPhe: 2.42 ± 0.215
4.064GlyGly: 4.064 ± 0.949
1.034GlyHis: 1.034 ± 0.142
4.041GlyIle: 4.041 ± 0.329
5.779GlyLys: 5.779 ± 0.436
4.698GlyLeu: 4.698 ± 0.332
1.41GlyMet: 1.41 ± 0.218
3.806GlyAsn: 3.806 ± 0.349
0.0GlyPro: 0.0 ± 0.0
1.997GlyGln: 1.997 ± 0.288
2.02GlyArg: 2.02 ± 0.25
4.346GlySer: 4.346 ± 0.386
3.688GlyThr: 3.688 ± 0.374
3.618GlyVal: 3.618 ± 0.301
0.634GlyTrp: 0.634 ± 0.134
3.383GlyTyr: 3.383 ± 0.27
0.023GlyXaa: 0.023 ± 0.021
His
0.611HisAla: 0.611 ± 0.106
0.235HisCys: 0.235 ± 0.086
0.94HisAsp: 0.94 ± 0.157
1.151HisGlu: 1.151 ± 0.168
0.752HisPhe: 0.752 ± 0.122
1.034HisGly: 1.034 ± 0.116
0.329HisHis: 0.329 ± 0.104
1.785HisIle: 1.785 ± 0.244
1.175HisLys: 1.175 ± 0.163
1.363HisLeu: 1.363 ± 0.18
0.282HisMet: 0.282 ± 0.09
1.104HisAsn: 1.104 ± 0.173
0.587HisPro: 0.587 ± 0.12
0.517HisGln: 0.517 ± 0.099
0.728HisArg: 0.728 ± 0.133
0.893HisSer: 0.893 ± 0.134
0.94HisThr: 0.94 ± 0.138
0.987HisVal: 0.987 ± 0.163
0.211HisTrp: 0.211 ± 0.085
0.775HisTyr: 0.775 ± 0.145
0.0HisXaa: 0.0 ± 0.0
Ile
3.336IleAla: 3.336 ± 0.316
0.399IleCys: 0.399 ± 0.102
5.756IleAsp: 5.756 ± 0.4
6.108IleGlu: 6.108 ± 0.463
2.467IlePhe: 2.467 ± 0.238
4.229IleGly: 4.229 ± 0.388
1.128IleHis: 1.128 ± 0.197
6.272IleIle: 6.272 ± 0.5
7.048IleLys: 7.048 ± 0.417
5.873IleLeu: 5.873 ± 0.426
1.832IleMet: 1.832 ± 0.2
5.638IleAsn: 5.638 ± 0.368
2.42IlePro: 2.42 ± 0.229
2.467IleGln: 2.467 ± 0.264
2.772IleArg: 2.772 ± 0.237
4.581IleSer: 4.581 ± 0.311
5.074IleThr: 5.074 ± 0.356
4.792IleVal: 4.792 ± 0.414
0.634IleTrp: 0.634 ± 0.104
3.078IleTyr: 3.078 ± 0.242
0.0IleXaa: 0.0 ± 0.0
Lys
3.923LysAla: 3.923 ± 0.371
0.399LysCys: 0.399 ± 0.107
7.306LysAsp: 7.306 ± 0.459
10.736LysGlu: 10.736 ± 0.68
3.054LysPhe: 3.054 ± 0.251
6.061LysGly: 6.061 ± 0.58
1.903LysHis: 1.903 ± 0.215
4.651LysIle: 4.651 ± 0.32
8.199LysLys: 8.199 ± 0.625
6.954LysLeu: 6.954 ± 0.429
2.443LysMet: 2.443 ± 0.239
5.615LysAsn: 5.615 ± 0.305
2.96LysPro: 2.96 ± 0.271
3.876LysGln: 3.876 ± 0.35
3.078LysArg: 3.078 ± 0.35
4.933LysSer: 4.933 ± 0.355
4.487LysThr: 4.487 ± 0.339
6.695LysVal: 6.695 ± 0.379
0.752LysTrp: 0.752 ± 0.139
4.558LysTyr: 4.558 ± 0.351
0.023LysXaa: 0.023 ± 0.021
Leu
3.5LeuAla: 3.5 ± 0.292
0.376LeuCys: 0.376 ± 0.095
5.779LeuAsp: 5.779 ± 0.385
7.095LeuGlu: 7.095 ± 0.52
2.913LeuPhe: 2.913 ± 0.239
4.816LeuGly: 4.816 ± 0.388
0.963LeuHis: 0.963 ± 0.159
5.638LeuIle: 5.638 ± 0.403
7.259LeuLys: 7.259 ± 0.423
6.319LeuLeu: 6.319 ± 0.503
2.044LeuMet: 2.044 ± 0.228
5.45LeuAsn: 5.45 ± 0.326
2.514LeuPro: 2.514 ± 0.25
2.913LeuGln: 2.913 ± 0.305
3.148LeuArg: 3.148 ± 0.248
5.991LeuSer: 5.991 ± 0.326
5.474LeuThr: 5.474 ± 0.355
4.605LeuVal: 4.605 ± 0.359
0.822LeuTrp: 0.822 ± 0.121
3.171LeuTyr: 3.171 ± 0.286
0.023LeuXaa: 0.023 ± 0.021
Met
1.715MetAla: 1.715 ± 0.227
0.188MetCys: 0.188 ± 0.077
1.809MetAsp: 1.809 ± 0.254
2.114MetGlu: 2.114 ± 0.219
1.175MetPhe: 1.175 ± 0.207
1.245MetGly: 1.245 ± 0.226
0.258MetHis: 0.258 ± 0.086
1.856MetIle: 1.856 ± 0.245
2.255MetLys: 2.255 ± 0.25
1.644MetLeu: 1.644 ± 0.182
0.681MetMet: 0.681 ± 0.13
1.762MetAsn: 1.762 ± 0.193
0.54MetPro: 0.54 ± 0.12
0.705MetGln: 0.705 ± 0.138
1.245MetArg: 1.245 ± 0.195
1.879MetSer: 1.879 ± 0.205
1.644MetThr: 1.644 ± 0.178
1.292MetVal: 1.292 ± 0.196
0.141MetTrp: 0.141 ± 0.052
1.01MetTyr: 1.01 ± 0.174
0.0MetXaa: 0.0 ± 0.0
Asn
2.772AsnAla: 2.772 ± 0.303
0.352AsnCys: 0.352 ± 0.092
4.158AsnAsp: 4.158 ± 0.335
4.605AsnGlu: 4.605 ± 0.333
2.514AsnPhe: 2.514 ± 0.224
3.923AsnGly: 3.923 ± 0.271
1.339AsnHis: 1.339 ± 0.175
5.121AsnIle: 5.121 ± 0.308
7.071AsnLys: 7.071 ± 0.425
5.38AsnLeu: 5.38 ± 0.365
1.644AsnMet: 1.644 ± 0.189
4.98AsnAsn: 4.98 ± 0.435
2.514AsnPro: 2.514 ± 0.286
1.926AsnGln: 1.926 ± 0.204
2.537AsnArg: 2.537 ± 0.225
3.994AsnSer: 3.994 ± 0.3
3.688AsnThr: 3.688 ± 0.287
4.017AsnVal: 4.017 ± 0.285
0.399AsnTrp: 0.399 ± 0.103
3.618AsnTyr: 3.618 ± 0.303
0.0AsnXaa: 0.0 ± 0.0
Pro
1.057ProAla: 1.057 ± 0.156
0.094ProCys: 0.094 ± 0.049
1.457ProAsp: 1.457 ± 0.239
2.49ProGlu: 2.49 ± 0.292
1.128ProPhe: 1.128 ± 0.172
1.128ProGly: 1.128 ± 0.195
0.446ProHis: 0.446 ± 0.11
2.091ProIle: 2.091 ± 0.205
2.467ProLys: 2.467 ± 0.206
1.903ProLeu: 1.903 ± 0.193
0.799ProMet: 0.799 ± 0.123
1.997ProAsn: 1.997 ± 0.217
0.681ProPro: 0.681 ± 0.15
1.198ProGln: 1.198 ± 0.199
0.893ProArg: 0.893 ± 0.131
2.396ProSer: 2.396 ± 0.321
1.809ProThr: 1.809 ± 0.204
1.574ProVal: 1.574 ± 0.209
0.164ProTrp: 0.164 ± 0.06
1.574ProTyr: 1.574 ± 0.211
0.0ProXaa: 0.0 ± 0.0
Gln
2.138GlnAla: 2.138 ± 0.227
0.094GlnCys: 0.094 ± 0.042
2.302GlnAsp: 2.302 ± 0.242
3.312GlnGlu: 3.312 ± 0.342
1.363GlnPhe: 1.363 ± 0.189
2.467GlnGly: 2.467 ± 0.314
0.587GlnHis: 0.587 ± 0.113
2.467GlnIle: 2.467 ± 0.251
2.279GlnLys: 2.279 ± 0.257
3.171GlnLeu: 3.171 ± 0.292
0.822GlnMet: 0.822 ± 0.139
1.504GlnAsn: 1.504 ± 0.208
1.175GlnPro: 1.175 ± 0.249
1.856GlnGln: 1.856 ± 0.372
1.128GlnArg: 1.128 ± 0.175
2.608GlnSer: 2.608 ± 0.248
1.856GlnThr: 1.856 ± 0.202
1.903GlnVal: 1.903 ± 0.202
0.235GlnTrp: 0.235 ± 0.069
1.926GlnTyr: 1.926 ± 0.209
0.0GlnXaa: 0.0 ± 0.0
Arg
1.48ArgAla: 1.48 ± 0.202
0.329ArgCys: 0.329 ± 0.102
2.608ArgAsp: 2.608 ± 0.213
3.054ArgGlu: 3.054 ± 0.246
1.668ArgPhe: 1.668 ± 0.218
2.161ArgGly: 2.161 ± 0.225
0.47ArgHis: 0.47 ± 0.096
2.608ArgIle: 2.608 ± 0.242
3.712ArgLys: 3.712 ± 0.302
3.031ArgLeu: 3.031 ± 0.22
0.893ArgMet: 0.893 ± 0.143
2.114ArgAsn: 2.114 ± 0.224
1.034ArgPro: 1.034 ± 0.157
1.433ArgGln: 1.433 ± 0.151
1.55ArgArg: 1.55 ± 0.195
1.691ArgSer: 1.691 ± 0.219
1.973ArgThr: 1.973 ± 0.23
2.302ArgVal: 2.302 ± 0.249
0.282ArgTrp: 0.282 ± 0.089
1.715ArgTyr: 1.715 ± 0.175
0.0ArgXaa: 0.0 ± 0.0
Ser
2.984SerAla: 2.984 ± 0.253
0.188SerCys: 0.188 ± 0.066
4.205SerAsp: 4.205 ± 0.344
4.323SerGlu: 4.323 ± 0.279
3.007SerPhe: 3.007 ± 0.265
3.9SerGly: 3.9 ± 0.445
1.034SerHis: 1.034 ± 0.159
5.121SerIle: 5.121 ± 0.384
6.296SerLys: 6.296 ± 0.402
5.403SerLeu: 5.403 ± 0.331
1.48SerMet: 1.48 ± 0.193
4.581SerAsn: 4.581 ± 0.354
1.903SerPro: 1.903 ± 0.22
1.762SerGln: 1.762 ± 0.234
2.067SerArg: 2.067 ± 0.215
4.581SerSer: 4.581 ± 0.417
4.064SerThr: 4.064 ± 0.377
4.252SerVal: 4.252 ± 0.327
0.634SerTrp: 0.634 ± 0.127
3.876SerTyr: 3.876 ± 0.29
0.023SerXaa: 0.023 ± 0.023
Thr
2.302ThrAla: 2.302 ± 0.241
0.094ThrCys: 0.094 ± 0.045
3.923ThrAsp: 3.923 ± 0.283
4.816ThrGlu: 4.816 ± 0.358
2.279ThrPhe: 2.279 ± 0.293
3.947ThrGly: 3.947 ± 0.347
0.916ThrHis: 0.916 ± 0.168
5.027ThrIle: 5.027 ± 0.388
4.675ThrLys: 4.675 ± 0.349
4.933ThrLeu: 4.933 ± 0.363
0.987ThrMet: 0.987 ± 0.127
3.054ThrAsn: 3.054 ± 0.268
2.044ThrPro: 2.044 ± 0.248
2.208ThrGln: 2.208 ± 0.254
2.279ThrArg: 2.279 ± 0.251
3.853ThrSer: 3.853 ± 0.306
3.007ThrThr: 3.007 ± 0.34
4.252ThrVal: 4.252 ± 0.422
0.611ThrTrp: 0.611 ± 0.136
2.655ThrTyr: 2.655 ± 0.219
0.023ThrXaa: 0.023 ± 0.023
Val
2.42ValAla: 2.42 ± 0.252
0.564ValCys: 0.564 ± 0.121
4.487ValAsp: 4.487 ± 0.306
5.286ValGlu: 5.286 ± 0.443
2.584ValPhe: 2.584 ± 0.216
3.078ValGly: 3.078 ± 0.233
1.104ValHis: 1.104 ± 0.158
4.675ValIle: 4.675 ± 0.328
6.132ValLys: 6.132 ± 0.47
5.239ValLeu: 5.239 ± 0.381
1.339ValMet: 1.339 ± 0.175
4.346ValAsn: 4.346 ± 0.333
1.597ValPro: 1.597 ± 0.16
1.738ValGln: 1.738 ± 0.25
2.302ValArg: 2.302 ± 0.237
4.792ValSer: 4.792 ± 0.384
3.806ValThr: 3.806 ± 0.39
4.041ValVal: 4.041 ± 0.351
0.376ValTrp: 0.376 ± 0.106
3.406ValTyr: 3.406 ± 0.312
0.0ValXaa: 0.0 ± 0.0
Trp
0.329TrpAla: 0.329 ± 0.083
0.047TrpCys: 0.047 ± 0.033
0.658TrpAsp: 0.658 ± 0.118
0.705TrpGlu: 0.705 ± 0.128
0.329TrpPhe: 0.329 ± 0.084
0.611TrpGly: 0.611 ± 0.134
0.141TrpHis: 0.141 ± 0.054
0.681TrpIle: 0.681 ± 0.159
0.775TrpLys: 0.775 ± 0.131
0.611TrpLeu: 0.611 ± 0.114
0.211TrpMet: 0.211 ± 0.068
0.705TrpAsn: 0.705 ± 0.131
0.0TrpPro: 0.0 ± 0.0
0.305TrpGln: 0.305 ± 0.083
0.211TrpArg: 0.211 ± 0.061
0.564TrpSer: 0.564 ± 0.103
0.399TrpThr: 0.399 ± 0.11
0.517TrpVal: 0.517 ± 0.106
0.141TrpTrp: 0.141 ± 0.067
0.564TrpTyr: 0.564 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.997TyrAla: 1.997 ± 0.241
0.376TyrCys: 0.376 ± 0.105
3.97TyrAsp: 3.97 ± 0.327
3.312TyrGlu: 3.312 ± 0.289
2.091TyrPhe: 2.091 ± 0.209
2.796TyrGly: 2.796 ± 0.273
0.893TyrHis: 0.893 ± 0.164
3.547TyrIle: 3.547 ± 0.319
4.933TyrLys: 4.933 ± 0.439
4.417TyrLeu: 4.417 ± 0.386
1.457TyrMet: 1.457 ± 0.209
4.229TyrAsn: 4.229 ± 0.331
1.222TyrPro: 1.222 ± 0.187
1.832TyrGln: 1.832 ± 0.199
1.738TyrArg: 1.738 ± 0.169
2.984TyrSer: 2.984 ± 0.263
2.749TyrThr: 2.749 ± 0.261
3.148TyrVal: 3.148 ± 0.306
0.399TyrTrp: 0.399 ± 0.119
3.148TyrTyr: 3.148 ± 0.298
0.023TyrXaa: 0.023 ± 0.021
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.023XaaGly: 0.023 ± 0.021
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.023XaaPro: 0.023 ± 0.025
0.023XaaGln: 0.023 ± 0.021
0.023XaaArg: 0.023 ± 0.021
0.023XaaSer: 0.023 ± 0.023
0.0XaaThr: 0.0 ± 0.0
0.07XaaVal: 0.07 ± 0.039
0.0XaaTrp: 0.0 ± 0.0
0.023XaaTyr: 0.023 ± 0.021
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 221 proteins (42568 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski