Acidobacteria bacterium AB60

Taxonomy: cellular organisms; Bacteria; Acidobacteria; unclassified Acidobacteria

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5367 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5M8SK51|A0A5M8SK51_9BACT Peroxiredoxin OS=Acidobacteria bacterium AB60 OX=1230846 GN=DYQ86_15425 PE=3 SV=1
MM1 pKa = 7.75ALGVLNNLSAIYY13 pKa = 10.65AEE15 pKa = 4.24NNLNNTSSSLQAVLRR30 pKa = 11.84QLSSGSRR37 pKa = 11.84INSGADD43 pKa = 3.18DD44 pKa = 4.03AAGLSLVNGLKK55 pKa = 10.79ANSTALAQSGTNATEE70 pKa = 4.2GVGLLQVADD79 pKa = 4.12GALSQVTSLLNRR91 pKa = 11.84AITLATEE98 pKa = 4.67ASNGTLNSSQDD109 pKa = 3.05AAADD113 pKa = 3.54QEE115 pKa = 4.61YY116 pKa = 10.75QSILAEE122 pKa = 4.13INNIGSTTTYY132 pKa = 9.01NQQRR136 pKa = 11.84VFGGGPVAIYY146 pKa = 9.86TGDD149 pKa = 3.56SSVTGASVDD158 pKa = 3.71LLNIRR163 pKa = 11.84GLSSQSVGDD172 pKa = 3.77SAGTMSYY179 pKa = 7.49TTGTNNVFIDD189 pKa = 4.23LSTAGKK195 pKa = 9.6YY196 pKa = 9.92AQTTDD201 pKa = 3.03SLAAGGSTSLEE212 pKa = 3.8VAYY215 pKa = 10.4LVAGTGTVANTTITVSGANNTVQGLIQAINNANLGITASFGTAAMAGAAAVAAVGGAANQSEE277 pKa = 4.51TGIIIAGNVNAGNAPTQAGYY297 pKa = 10.25HH298 pKa = 5.52VGIFQDD304 pKa = 3.4ATAAATDD311 pKa = 4.04LLYY314 pKa = 10.86DD315 pKa = 3.76SSVGQTFNLGVAPAASTAPQTSFTQDD341 pKa = 2.8LNAASIATISYY352 pKa = 8.61TDD354 pKa = 3.36AAGVSLSGTDD364 pKa = 3.74LKK366 pKa = 11.4SSTSAQAALSLLNQAISAVAAQDD389 pKa = 4.01GYY391 pKa = 10.84IGAQINTLNSISQVMSTQQEE411 pKa = 4.47NVVSAQNAIQATDD424 pKa = 3.54YY425 pKa = 11.45AAATSNMSKK434 pKa = 10.86FEE436 pKa = 4.18ILSQTGIAALAQANQVEE453 pKa = 4.37QEE455 pKa = 4.35VIKK458 pKa = 10.76LLQQ461 pKa = 3.54

Molecular weight: 45.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5M8SR00|A0A5M8SR00_9BACT DUF5110 domain-containing protein OS=Acidobacteria bacterium AB60 OX=1230846 GN=DYQ86_10940 PE=3 SV=1
MM1 pKa = 7.56EE2 pKa = 5.24ARR4 pKa = 11.84FEE6 pKa = 3.96QFIRR10 pKa = 11.84EE11 pKa = 3.89RR12 pKa = 11.84QYY14 pKa = 11.32LQNVTPRR21 pKa = 11.84TIEE24 pKa = 3.58WYY26 pKa = 10.04RR27 pKa = 11.84FSLRR31 pKa = 11.84WLPCEE36 pKa = 4.39DD37 pKa = 3.55PTRR40 pKa = 11.84AQLDD44 pKa = 3.7EE45 pKa = 4.22MVVRR49 pKa = 11.84MRR51 pKa = 11.84EE52 pKa = 3.86RR53 pKa = 11.84GLKK56 pKa = 8.38PTGCNCLCRR65 pKa = 11.84AINAYY70 pKa = 9.88LKK72 pKa = 10.32WSGSTHH78 pKa = 7.29KK79 pKa = 10.15IRR81 pKa = 11.84PFKK84 pKa = 10.9EE85 pKa = 3.94PDD87 pKa = 3.15SVLPIFTEE95 pKa = 4.11QQFKK99 pKa = 10.88RR100 pKa = 11.84LIAWKK105 pKa = 9.46PKK107 pKa = 9.94SKK109 pKa = 9.93YY110 pKa = 7.05QRR112 pKa = 11.84RR113 pKa = 11.84LHH115 pKa = 6.46LLILFLLDD123 pKa = 3.22TGCRR127 pKa = 11.84ISEE130 pKa = 4.03ALGLRR135 pKa = 11.84VSEE138 pKa = 4.29IDD140 pKa = 4.61LDD142 pKa = 4.01NLLVKK147 pKa = 10.64LDD149 pKa = 3.66GKK151 pKa = 10.23GRR153 pKa = 11.84KK154 pKa = 7.56QRR156 pKa = 11.84IVPISLEE163 pKa = 3.74LRR165 pKa = 11.84KK166 pKa = 10.18AIVRR170 pKa = 11.84FCTDD174 pKa = 3.11RR175 pKa = 11.84ARR177 pKa = 11.84KK178 pKa = 8.94PDD180 pKa = 3.7LLLFASRR187 pKa = 11.84VEE189 pKa = 4.47TTWVRR194 pKa = 11.84RR195 pKa = 11.84NILRR199 pKa = 11.84DD200 pKa = 3.39VKK202 pKa = 10.45RR203 pKa = 11.84VCRR206 pKa = 11.84TLGFEE211 pKa = 3.98PPIRR215 pKa = 11.84TLHH218 pKa = 6.23AFRR221 pKa = 11.84HH222 pKa = 5.02TFAVNYY228 pKa = 9.02LRR230 pKa = 11.84RR231 pKa = 11.84GGSVFHH237 pKa = 5.81LQKK240 pKa = 10.33MLGHH244 pKa = 6.1STLEE248 pKa = 3.68MTRR251 pKa = 11.84RR252 pKa = 11.84YY253 pKa = 10.59ANLVTADD260 pKa = 3.77LQAVHH265 pKa = 6.37EE266 pKa = 4.41RR267 pKa = 11.84LSLLSRR273 pKa = 4.14

Molecular weight: 32.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5367

0

5367

1893627

29

3493

352.8

38.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.343 ± 0.043

0.923 ± 0.013

5.043 ± 0.023

5.461 ± 0.04

3.844 ± 0.021

8.24 ± 0.036

2.274 ± 0.017

4.755 ± 0.021

3.316 ± 0.028

10.014 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.013

3.271 ± 0.031

5.607 ± 0.026

3.827 ± 0.022

6.623 ± 0.039

6.226 ± 0.032

5.745 ± 0.044

7.187 ± 0.027

1.48 ± 0.016

2.636 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski