Amino acid dipepetide frequency for Acidobacteria bacterium AB60

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.727AlaAla: 14.727 ± 0.122	1.109AlaCys: 1.109 ± 0.026	5.266AlaAsp: 5.266 ± 0.055	6.358AlaGlu: 6.358 ± 0.093	3.937AlaPhe: 3.937 ± 0.044	9.818AlaGly: 9.818 ± 0.094	2.392AlaHis: 2.392 ± 0.04	5.637AlaIle: 5.637 ± 0.059	3.589AlaLys: 3.589 ± 0.053	11.494AlaLeu: 11.494 ± 0.104	2.732AlaMet: 2.732 ± 0.047	3.47AlaAsn: 3.47 ± 0.059	5.727AlaPro: 5.727 ± 0.074	4.664AlaGln: 4.664 ± 0.056	7.282AlaArg: 7.282 ± 0.086	6.832AlaSer: 6.832 ± 0.074	6.154AlaThr: 6.154 ± 0.08	8.136AlaVal: 8.136 ± 0.076	1.58AlaTrp: 1.58 ± 0.031	2.527AlaTyr: 2.527 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.067CysAla: 1.067 ± 0.028	0.133CysCys: 0.133 ± 0.009	0.471CysAsp: 0.471 ± 0.015	0.401CysGlu: 0.401 ± 0.017	0.341CysPhe: 0.341 ± 0.012	0.992CysGly: 0.992 ± 0.026	0.324CysHis: 0.324 ± 0.031	0.362CysIle: 0.362 ± 0.015	0.228CysLys: 0.228 ± 0.011	0.817CysLeu: 0.817 ± 0.021	0.189CysMet: 0.189 ± 0.01	0.289CysAsn: 0.289 ± 0.014	0.502CysPro: 0.502 ± 0.017	0.272CysGln: 0.272 ± 0.012	0.571CysArg: 0.571 ± 0.019	0.653CysSer: 0.653 ± 0.019	0.557CysThr: 0.557 ± 0.022	0.655CysVal: 0.655 ± 0.021	0.141CysTrp: 0.141 ± 0.01	0.266CysTyr: 0.266 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	5.607AspAla: 5.607 ± 0.058	0.475AspCys: 0.475 ± 0.018	2.333AspAsp: 2.333 ± 0.042	2.888AspGlu: 2.888 ± 0.047	2.136AspPhe: 2.136 ± 0.037	4.466AspGly: 4.466 ± 0.064	1.214AspHis: 1.214 ± 0.027	2.119AspIle: 2.119 ± 0.035	1.549AspLys: 1.549 ± 0.033	5.26AspLeu: 5.26 ± 0.065	0.899AspMet: 0.899 ± 0.023	1.331AspAsn: 1.331 ± 0.027	3.677AspPro: 3.677 ± 0.043	1.974AspGln: 1.974 ± 0.033	3.411AspArg: 3.411 ± 0.049	2.691AspSer: 2.691 ± 0.041	2.459AspThr: 2.459 ± 0.036	3.504AspVal: 3.504 ± 0.047	0.917AspTrp: 0.917 ± 0.022	1.516AspTyr: 1.516 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	5.958GluAla: 5.958 ± 0.078	0.41GluCys: 0.41 ± 0.016	2.515GluAsp: 2.515 ± 0.041	3.344GluGlu: 3.344 ± 0.062	2.056GluPhe: 2.056 ± 0.039	3.822GluGly: 3.822 ± 0.055	1.365GluHis: 1.365 ± 0.029	3.115GluIle: 3.115 ± 0.047	2.308GluLys: 2.308 ± 0.044	5.413GluLeu: 5.413 ± 0.078	1.46GluMet: 1.46 ± 0.03	1.663GluAsn: 1.663 ± 0.035	2.568GluPro: 2.568 ± 0.043	2.437GluGln: 2.437 ± 0.043	4.274GluArg: 4.274 ± 0.068	3.149GluSer: 3.149 ± 0.042	2.937GluThr: 2.937 ± 0.041	3.662GluVal: 3.662 ± 0.052	0.782GluTrp: 0.782 ± 0.023	1.369GluTyr: 1.369 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.348PheAla: 4.348 ± 0.049	0.398PheCys: 0.398 ± 0.013	2.385PheAsp: 2.385 ± 0.038	2.05PheGlu: 2.05 ± 0.036	1.61PhePhe: 1.61 ± 0.029	3.415PheGly: 3.415 ± 0.046	0.98PheHis: 0.98 ± 0.024	1.313PheIle: 1.313 ± 0.03	1.035PheLys: 1.035 ± 0.024	3.888PheLeu: 3.888 ± 0.056	0.635PheMet: 0.635 ± 0.019	1.4PheAsn: 1.4 ± 0.034	1.898PhePro: 1.898 ± 0.032	1.318PheGln: 1.318 ± 0.027	2.38PheArg: 2.38 ± 0.041	2.626PheSer: 2.626 ± 0.04	2.418PheThr: 2.418 ± 0.044	2.67PheVal: 2.67 ± 0.039	0.569PheTrp: 0.569 ± 0.019	1.106PheTyr: 1.106 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	8.329GlyAla: 8.329 ± 0.088	0.879GlyCys: 0.879 ± 0.023	4.075GlyAsp: 4.075 ± 0.057	4.14GlyGlu: 4.14 ± 0.05	3.533GlyPhe: 3.533 ± 0.047	6.727GlyGly: 6.727 ± 0.113	1.817GlyHis: 1.817 ± 0.032	4.419GlyIle: 4.419 ± 0.049	3.514GlyLys: 3.514 ± 0.048	7.65GlyLeu: 7.65 ± 0.062	2.069GlyMet: 2.069 ± 0.037	2.883GlyAsn: 2.883 ± 0.063	3.589GlyPro: 3.589 ± 0.053	3.017GlyGln: 3.017 ± 0.042	5.017GlyArg: 5.017 ± 0.061	5.536GlySer: 5.536 ± 0.075	5.222GlyThr: 5.222 ± 0.08	6.036GlyVal: 6.036 ± 0.065	1.48GlyTrp: 1.48 ± 0.03	2.465GlyTyr: 2.465 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.418HisAla: 2.418 ± 0.041	0.224HisCys: 0.224 ± 0.012	1.198HisAsp: 1.198 ± 0.026	1.173HisGlu: 1.173 ± 0.028	1.002HisPhe: 1.002 ± 0.025	2.025HisGly: 2.025 ± 0.036	0.608HisHis: 0.608 ± 0.017	1.03HisIle: 1.03 ± 0.028	0.591HisLys: 0.591 ± 0.015	2.328HisLeu: 2.328 ± 0.04	0.471HisMet: 0.471 ± 0.018	0.698HisAsn: 0.698 ± 0.023	1.561HisPro: 1.561 ± 0.029	0.792HisGln: 0.792 ± 0.017	1.502HisArg: 1.502 ± 0.03	1.223HisSer: 1.223 ± 0.028	1.143HisThr: 1.143 ± 0.028	1.634HisVal: 1.634 ± 0.031	0.422HisTrp: 0.422 ± 0.015	0.697HisTyr: 0.697 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	6.215IleAla: 6.215 ± 0.06	0.452IleCys: 0.452 ± 0.015	2.87IleAsp: 2.87 ± 0.04	2.975IleGlu: 2.975 ± 0.052	1.733IlePhe: 1.733 ± 0.03	3.953IleGly: 3.953 ± 0.05	1.124IleHis: 1.124 ± 0.024	1.593IleIle: 1.593 ± 0.032	1.291IleLys: 1.291 ± 0.027	4.497IleLeu: 4.497 ± 0.058	0.661IleMet: 0.661 ± 0.017	1.474IleAsn: 1.474 ± 0.029	2.812IlePro: 2.812 ± 0.042	1.652IleGln: 1.652 ± 0.036	3.012IleArg: 3.012 ± 0.041	3.008IleSer: 3.008 ± 0.048	2.902IleThr: 2.902 ± 0.045	3.517IleVal: 3.517 ± 0.044	0.555IleTrp: 0.555 ± 0.018	1.251IleTyr: 1.251 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	3.508LysAla: 3.508 ± 0.053	0.214LysCys: 0.214 ± 0.011	1.699LysAsp: 1.699 ± 0.039	1.766LysGlu: 1.766 ± 0.04	1.043LysPhe: 1.043 ± 0.023	2.428LysGly: 2.428 ± 0.035	0.751LysHis: 0.751 ± 0.021	1.691LysIle: 1.691 ± 0.031	1.435LysLys: 1.435 ± 0.037	3.399LysLeu: 3.399 ± 0.049	0.87LysMet: 0.87 ± 0.023	1.113LysAsn: 1.113 ± 0.026	2.159LysPro: 2.159 ± 0.039	1.332LysGln: 1.332 ± 0.027	2.11LysArg: 2.11 ± 0.042	1.968LysSer: 1.968 ± 0.033	2.044LysThr: 2.044 ± 0.036	2.366LysVal: 2.366 ± 0.041	0.42LysTrp: 0.42 ± 0.017	0.842LysTyr: 0.842 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	11.966LeuAla: 11.966 ± 0.106	0.937LeuCys: 0.937 ± 0.022	5.243LeuAsp: 5.243 ± 0.064	5.537LeuGlu: 5.537 ± 0.08	3.618LeuPhe: 3.618 ± 0.051	7.754LeuGly: 7.754 ± 0.071	2.274LeuHis: 2.274 ± 0.039	4.391LeuIle: 4.391 ± 0.052	3.533LeuLys: 3.533 ± 0.048	10.439LeuLeu: 10.439 ± 0.103	1.924LeuMet: 1.924 ± 0.038	3.266LeuAsn: 3.266 ± 0.049	5.686LeuPro: 5.686 ± 0.059	3.505LeuGln: 3.505 ± 0.048	7.339LeuArg: 7.339 ± 0.088	6.213LeuSer: 6.213 ± 0.057	5.87LeuThr: 5.87 ± 0.066	6.911LeuVal: 6.911 ± 0.065	1.307LeuTrp: 1.307 ± 0.033	2.427LeuTyr: 2.427 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.51MetAla: 2.51 ± 0.039	0.161MetCys: 0.161 ± 0.009	1.007MetAsp: 1.007 ± 0.024	1.118MetGlu: 1.118 ± 0.027	0.622MetPhe: 0.622 ± 0.022	1.659MetGly: 1.659 ± 0.03	0.513MetHis: 0.513 ± 0.016	1.01MetIle: 1.01 ± 0.024	0.95MetLys: 0.95 ± 0.02	2.101MetLeu: 2.101 ± 0.043	0.544MetMet: 0.544 ± 0.017	0.814MetAsn: 0.814 ± 0.021	1.405MetPro: 1.405 ± 0.027	0.917MetGln: 0.917 ± 0.023	1.729MetArg: 1.729 ± 0.037	1.345MetSer: 1.345 ± 0.028	1.394MetThr: 1.394 ± 0.029	1.461MetVal: 1.461 ± 0.033	0.219MetTrp: 0.219 ± 0.013	0.389MetTyr: 0.389 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.47AsnAla: 3.47 ± 0.056	0.34AsnCys: 0.34 ± 0.015	1.535AsnAsp: 1.535 ± 0.034	1.493AsnGlu: 1.493 ± 0.03	1.294AsnPhe: 1.294 ± 0.032	3.19AsnGly: 3.19 ± 0.069	0.708AsnHis: 0.708 ± 0.021	1.436AsnIle: 1.436 ± 0.034	0.805AsnLys: 0.805 ± 0.022	3.347AsnLeu: 3.347 ± 0.05	0.563AsnMet: 0.563 ± 0.015	1.155AsnAsn: 1.155 ± 0.04	2.475AsnPro: 2.475 ± 0.045	1.269AsnGln: 1.269 ± 0.027	1.977AsnArg: 1.977 ± 0.03	1.983AsnSer: 1.983 ± 0.047	1.822AsnThr: 1.822 ± 0.049	2.258AsnVal: 2.258 ± 0.036	0.551AsnTrp: 0.551 ± 0.02	1.037AsnTyr: 1.037 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	7.095ProAla: 7.095 ± 0.073	0.408ProCys: 0.408 ± 0.015	3.326ProAsp: 3.326 ± 0.043	3.697ProGlu: 3.697 ± 0.057	1.985ProPhe: 1.985 ± 0.03	5.262ProGly: 5.262 ± 0.067	1.197ProHis: 1.197 ± 0.024	2.358ProIle: 2.358 ± 0.04	1.661ProLys: 1.661 ± 0.033	4.932ProLeu: 4.932 ± 0.061	1.123ProMet: 1.123 ± 0.024	1.896ProAsn: 1.896 ± 0.041	3.11ProPro: 3.11 ± 0.054	2.265ProGln: 2.265 ± 0.044	3.019ProArg: 3.019 ± 0.042	3.502ProSer: 3.502 ± 0.049	2.821ProThr: 2.821 ± 0.057	4.301ProVal: 4.301 ± 0.043	0.748ProTrp: 0.748 ± 0.019	1.358ProTyr: 1.358 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	4.248GlnAla: 4.248 ± 0.054	0.293GlnCys: 0.293 ± 0.016	1.575GlnAsp: 1.575 ± 0.03	1.902GlnGlu: 1.902 ± 0.036	1.468GlnPhe: 1.468 ± 0.028	2.694GlnGly: 2.694 ± 0.044	0.87GlnHis: 0.87 ± 0.023	2.102GlnIle: 2.102 ± 0.034	1.357GlnLys: 1.357 ± 0.029	3.67GlnLeu: 3.67 ± 0.048	1.059GlnMet: 1.059 ± 0.023	1.286GlnAsn: 1.286 ± 0.032	2.258GlnPro: 2.258 ± 0.035	1.966GlnGln: 1.966 ± 0.047	2.626GlnArg: 2.626 ± 0.038	2.411GlnSer: 2.411 ± 0.039	2.212GlnThr: 2.212 ± 0.04	2.76GlnVal: 2.76 ± 0.042	0.538GlnTrp: 0.538 ± 0.017	0.971GlnTyr: 0.971 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	6.633ArgAla: 6.633 ± 0.086	0.537ArgCys: 0.537 ± 0.019	3.346ArgAsp: 3.346 ± 0.049	4.138ArgGlu: 4.138 ± 0.065	2.711ArgPhe: 2.711 ± 0.04	4.336ArgGly: 4.336 ± 0.055	1.482ArgHis: 1.482 ± 0.029	3.763ArgIle: 3.763 ± 0.055	2.385ArgLys: 2.385 ± 0.05	6.867ArgLeu: 6.867 ± 0.086	1.875ArgMet: 1.875 ± 0.034	2.143ArgAsn: 2.143 ± 0.037	3.165ArgPro: 3.165 ± 0.043	2.558ArgGln: 2.558 ± 0.041	5.067ArgArg: 5.067 ± 0.064	3.943ArgSer: 3.943 ± 0.052	3.535ArgThr: 3.535 ± 0.044	4.726ArgVal: 4.726 ± 0.064	1.113ArgTrp: 1.113 ± 0.028	1.909ArgTyr: 1.909 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.93SerAla: 6.93 ± 0.076	0.628SerCys: 0.628 ± 0.024	2.961SerAsp: 2.961 ± 0.038	2.846SerGlu: 2.846 ± 0.05	2.522SerPhe: 2.522 ± 0.039	5.978SerGly: 5.978 ± 0.086	1.304SerHis: 1.304 ± 0.027	3.079SerIle: 3.079 ± 0.04	1.855SerLys: 1.855 ± 0.033	6.283SerLeu: 6.283 ± 0.056	1.336SerMet: 1.336 ± 0.03	1.99SerAsn: 1.99 ± 0.046	3.611SerPro: 3.611 ± 0.052	2.211SerGln: 2.211 ± 0.036	3.831SerArg: 3.831 ± 0.052	4.374SerSer: 4.374 ± 0.075	3.546SerThr: 3.546 ± 0.067	4.387SerVal: 4.387 ± 0.056	0.929SerTrp: 0.929 ± 0.024	1.654SerTyr: 1.654 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	6.486ThrAla: 6.486 ± 0.081	0.526ThrCys: 0.526 ± 0.021	2.647ThrAsp: 2.647 ± 0.041	2.653ThrGlu: 2.653 ± 0.038	2.267ThrPhe: 2.267 ± 0.046	5.461ThrGly: 5.461 ± 0.083	1.209ThrHis: 1.209 ± 0.026	2.977ThrIle: 2.977 ± 0.044	1.411ThrLys: 1.411 ± 0.026	5.935ThrLeu: 5.935 ± 0.066	1.116ThrMet: 1.116 ± 0.025	1.811ThrAsn: 1.811 ± 0.053	3.771ThrPro: 3.771 ± 0.052	1.945ThrGln: 1.945 ± 0.037	3.272ThrArg: 3.272 ± 0.046	3.44ThrSer: 3.44 ± 0.062	3.431ThrThr: 3.431 ± 0.074	4.677ThrVal: 4.677 ± 0.064	0.875ThrTrp: 0.875 ± 0.024	1.539ThrTyr: 1.539 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	7.976ValAla: 7.976 ± 0.075	0.717ValCys: 0.717 ± 0.019	3.777ValAsp: 3.777 ± 0.048	4.068ValGlu: 4.068 ± 0.063	2.678ValPhe: 2.678 ± 0.046	5.066ValGly: 5.066 ± 0.058	1.573ValHis: 1.573 ± 0.03	3.324ValIle: 3.324 ± 0.041	2.234ValLys: 2.234 ± 0.038	7.68ValLeu: 7.68 ± 0.083	1.484ValMet: 1.484 ± 0.029	2.498ValAsn: 2.498 ± 0.044	4.018ValPro: 4.018 ± 0.05	2.507ValGln: 2.507 ± 0.036	4.808ValArg: 4.808 ± 0.064	4.685ValSer: 4.685 ± 0.056	4.545ValThr: 4.545 ± 0.073	5.475ValVal: 5.475 ± 0.06	0.947ValTrp: 0.947 ± 0.024	1.811ValTyr: 1.811 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.256TrpAla: 1.256 ± 0.027	0.137TrpCys: 0.137 ± 0.009	0.693TrpAsp: 0.693 ± 0.023	0.694TrpGlu: 0.694 ± 0.02	0.625TrpPhe: 0.625 ± 0.021	1.068TrpGly: 1.068 ± 0.024	0.393TrpHis: 0.393 ± 0.017	0.844TrpIle: 0.844 ± 0.024	0.638TrpLys: 0.638 ± 0.02	1.528TrpLeu: 1.528 ± 0.033	0.425TrpMet: 0.425 ± 0.015	0.626TrpAsn: 0.626 ± 0.023	0.687TrpPro: 0.687 ± 0.02	0.653TrpGln: 0.653 ± 0.02	1.071TrpArg: 1.071 ± 0.024	0.977TrpSer: 0.977 ± 0.024	0.9TrpThr: 0.9 ± 0.021	0.945TrpVal: 0.945 ± 0.025	0.254TrpTrp: 0.254 ± 0.01	0.389TrpTyr: 0.389 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.684TyrAla: 2.684 ± 0.039	0.256TyrCys: 0.256 ± 0.013	1.504TyrAsp: 1.504 ± 0.033	1.364TyrGlu: 1.364 ± 0.025	1.261TyrPhe: 1.261 ± 0.029	2.281TyrGly: 2.281 ± 0.043	0.642TyrHis: 0.642 ± 0.019	0.982TyrIle: 0.982 ± 0.024	0.79TyrLys: 0.79 ± 0.022	2.612TyrLeu: 2.612 ± 0.044	0.43TyrMet: 0.43 ± 0.016	0.902TyrAsn: 0.902 ± 0.031	1.386TyrPro: 1.386 ± 0.03	1.011TyrGln: 1.011 ± 0.023	1.931TyrArg: 1.931 ± 0.034	1.695TyrSer: 1.695 ± 0.032	1.54TyrThr: 1.54 ± 0.036	1.792TyrVal: 1.792 ± 0.033	0.456TyrTrp: 0.456 ± 0.016	0.835TyrTyr: 0.835 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5367 proteins (1893627 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---