Leptolyngbya sp. O-77

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Leptolyngbyaceae; Leptolyngbya; unclassified Leptolyngbya

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4814 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X8WYE5|A0A0X8WYE5_9CYAN Putative FAD-linked oxidoreductase OS=Leptolyngbya sp. O-77 OX=1080068 GN=O77CONTIG1_03706 PE=4 SV=1
MM1 pKa = 8.32DD2 pKa = 4.72IGDD5 pKa = 4.67LVRR8 pKa = 11.84LKK10 pKa = 10.68QPFQPEE16 pKa = 3.93PDD18 pKa = 3.28SDD20 pKa = 3.23RR21 pKa = 11.84TYY23 pKa = 11.09SYY25 pKa = 11.13GIIAGIVWSEE35 pKa = 4.19DD36 pKa = 2.9ASLPSPPVEE45 pKa = 3.61IVLYY49 pKa = 10.62LYY51 pKa = 10.99DD52 pKa = 4.89LDD54 pKa = 3.85TRR56 pKa = 11.84QIYY59 pKa = 10.18VDD61 pKa = 3.72SAGLQAIYY69 pKa = 10.72AFRR72 pKa = 11.84ADD74 pKa = 3.76EE75 pKa = 4.5LEE77 pKa = 4.26LVV79 pKa = 3.8

Molecular weight: 8.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A125STS1|A0A125STS1_9CYAN Uncharacterized protein OS=Leptolyngbya sp. O-77 OX=1080068 GN=O77CONTIG1_04858 PE=4 SV=1
MM1 pKa = 7.6SIAGNLTVYY10 pKa = 10.45LAVYY14 pKa = 9.43SAGAIAPAAPEE25 pKa = 3.88AAGTFAKK32 pKa = 10.05TSLDD36 pKa = 3.36WQVQRR41 pKa = 11.84SLRR44 pKa = 11.84QVGEE48 pKa = 3.91WIEE51 pKa = 3.87WVLFGEE57 pKa = 4.77SPNRR61 pKa = 11.84PNFSGWIPPEE71 pKa = 3.72WVLQALFWVIVGLLGIWAAWWLVQWLRR98 pKa = 11.84PYY100 pKa = 9.92WEE102 pKa = 4.25AFQRR106 pKa = 11.84QQGRR110 pKa = 11.84AIAPRR115 pKa = 11.84APQPQRR121 pKa = 11.84RR122 pKa = 11.84NSALRR127 pKa = 11.84NGWRR131 pKa = 11.84GHH133 pKa = 5.86RR134 pKa = 11.84RR135 pKa = 11.84PSAKK139 pKa = 9.48ATIEE143 pKa = 4.02KK144 pKa = 10.2LAEE147 pKa = 3.82RR148 pKa = 11.84CIMPRR153 pKa = 11.84CIDD156 pKa = 3.16

Molecular weight: 17.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4814

0

4814

1474271

29

2703

306.2

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.001 ± 0.041

0.962 ± 0.012

5.249 ± 0.029

5.81 ± 0.035

3.623 ± 0.024

7.316 ± 0.039

1.908 ± 0.022

5.245 ± 0.024

3.043 ± 0.029

11.526 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.963 ± 0.016

3.13 ± 0.03

5.736 ± 0.038

5.212 ± 0.031

6.474 ± 0.031

6.184 ± 0.03

5.481 ± 0.032

6.934 ± 0.03

1.508 ± 0.018

2.695 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski