Mycobacterium phage Xeno
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142K7M4|A0A142K7M4_9CAUD Uncharacterized protein OS=Mycobacterium phage Xeno OX=1821538 GN=23 PE=4 SV=1
MM1 pKa = 7.49 EE2 pKa = 5.87 LFVPCPLCGSDD13 pKa = 3.44 VAVPLLSEE21 pKa = 4.51 GDD23 pKa = 3.52 HH24 pKa = 5.9 MRR26 pKa = 11.84 APDD29 pKa = 4.57 LLPNMNSHH37 pKa = 6.92 VISVHH42 pKa = 5.19 EE43 pKa = 4.08 MDD45 pKa = 3.8 VSGMM49 pKa = 3.65
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.059
IPC2_protein 4.431
IPC_protein 4.215
Toseland 4.062
ProMoST 4.393
Dawson 4.215
Bjellqvist 4.368
Wikipedia 4.151
Rodwell 4.075
Grimsley 3.986
Solomon 4.19
Lehninger 4.151
Nozaki 4.355
DTASelect 4.52
Thurlkill 4.126
EMBOSS 4.164
Sillero 4.355
Patrickios 2.003
IPC_peptide 4.19
IPC2_peptide 4.342
IPC2.peptide.svr19 4.228
Protein with the highest isoelectric point:
>tr|A0A142K7N9|A0A142K7N9_9CAUD Uncharacterized protein OS=Mycobacterium phage Xeno OX=1821538 GN=38 PE=4 SV=1
MM1 pKa = 7.31 TGLVTALLLVQVLTLLVQLSHH22 pKa = 6.98 AGATQRR28 pKa = 11.84 VLSLLIAASRR38 pKa = 11.84 RR39 pKa = 11.84 VRR41 pKa = 3.31
Molecular weight: 4.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 10.701
IPC_protein 12.31
Toseland 12.471
ProMoST 12.998
Dawson 12.471
Bjellqvist 12.471
Wikipedia 12.954
Rodwell 11.974
Grimsley 12.515
Solomon 12.983
Lehninger 12.881
Nozaki 12.471
DTASelect 12.471
Thurlkill 12.471
EMBOSS 12.983
Sillero 12.471
Patrickios 11.798
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
69
0
69
13788
41
1046
199.8
21.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.148 ± 0.571
1.124 ± 0.171
6.556 ± 0.211
5.875 ± 0.375
2.8 ± 0.157
8.964 ± 0.55
1.915 ± 0.163
4.54 ± 0.205
3.061 ± 0.231
7.731 ± 0.244
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.002 ± 0.157
3.162 ± 0.208
6.121 ± 0.252
3.655 ± 0.208
6.963 ± 0.433
5.222 ± 0.229
6.876 ± 0.252
7.064 ± 0.276
1.871 ± 0.116
2.35 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here