Armillaria gallica (Bulbous honey fungus) (Armillaria bulbosa)
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 25522 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H3ERP8|A0A2H3ERP8_ARMGA Uncharacterized protein OS=Armillaria gallica OX=47427 GN=ARMGADRAFT_1094090 PE=4 SV=1
MM1 pKa = 7.27 VFRR4 pKa = 11.84 TFAVFSLFSWAAAVANPLASGDD26 pKa = 3.45 FWTSIEE32 pKa = 4.33 DD33 pKa = 3.52 LRR35 pKa = 11.84 GPADD39 pKa = 3.63 SIYY42 pKa = 10.52 RR43 pKa = 11.84 DD44 pKa = 3.47 STLLRR49 pKa = 11.84 IQPTAASANTLLNDD63 pKa = 3.15 VTAFNSEE70 pKa = 3.83 VQAALTQIPATVMSEE85 pKa = 3.69 EE86 pKa = 4.9 DD87 pKa = 3.13 ADD89 pKa = 3.78 STYY92 pKa = 10.14 RR93 pKa = 11.84 TFLDD97 pKa = 3.16 IYY99 pKa = 9.89 EE100 pKa = 4.36 DD101 pKa = 3.46 TRR103 pKa = 11.84 SAFNALNEE111 pKa = 4.25 AEE113 pKa = 4.86 SYY115 pKa = 10.96 LEE117 pKa = 3.95 PFGLVPTICGLTQSIQTGLHH137 pKa = 4.95 TLVNEE142 pKa = 4.44 TLAVTPEE149 pKa = 4.66 DD150 pKa = 3.98 YY151 pKa = 10.98 VSPIQTVTGPILDD164 pKa = 4.48 AVDD167 pKa = 3.58 SAINFYY173 pKa = 10.95 CC174 pKa = 4.91
Molecular weight: 18.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.567
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.884
Patrickios 0.896
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.781
Protein with the highest isoelectric point:
>tr|A0A2H3CJU4|A0A2H3CJU4_ARMGA Uncharacterized protein OS=Armillaria gallica OX=47427 GN=ARMGADRAFT_1037963 PE=4 SV=1
MM1 pKa = 7.84 LPRR4 pKa = 11.84 CQWRR8 pKa = 11.84 FRR10 pKa = 11.84 LVSSTPLRR18 pKa = 11.84 TSGGLTFTSKK28 pKa = 10.9 AFRR31 pKa = 11.84 KK32 pKa = 9.53 SLQACSPMSDD42 pKa = 3.0 CRR44 pKa = 11.84 FSINATKK51 pKa = 8.63 YY52 pKa = 7.74 TKK54 pKa = 9.17 HH55 pKa = 5.73 TLLVYY60 pKa = 9.78 TSHH63 pKa = 6.97 IQDD66 pKa = 3.69 PQVSTKK72 pKa = 8.45 SRR74 pKa = 11.84 RR75 pKa = 11.84 ISTRR79 pKa = 11.84 CHH81 pKa = 5.75 HH82 pKa = 6.98 ASTTTVLSTFSSCSLAVFRR101 pKa = 11.84 SFLSSVVFTPPALSSHH117 pKa = 6.35 TKK119 pKa = 10.02 NAMGQIEE126 pKa = 5.12 AYY128 pKa = 9.53 LAKK131 pKa = 10.24 DD132 pKa = 4.27 LKK134 pKa = 10.82 RR135 pKa = 11.84 EE136 pKa = 4.13 PYY138 pKa = 10.06 DD139 pKa = 3.43 VRR141 pKa = 11.84 SSDD144 pKa = 3.21 ISLTRR149 pKa = 11.84 HH150 pKa = 5.32 RR151 pKa = 4.89
Molecular weight: 17.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.692
IPC_protein 10.365
Toseland 10.628
ProMoST 10.467
Dawson 10.73
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 11.008
Grimsley 10.789
Solomon 10.818
Lehninger 10.789
Nozaki 10.643
DTASelect 10.423
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.672
Patrickios 10.745
IPC_peptide 10.833
IPC2_peptide 9.648
IPC2.peptide.svr19 8.559
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
25522
0
25522
8654150
49
4990
339.1
37.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.648 ± 0.013
1.59 ± 0.008
5.657 ± 0.012
5.611 ± 0.016
3.953 ± 0.01
6.12 ± 0.014
2.692 ± 0.008
5.397 ± 0.012
4.491 ± 0.013
9.545 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.28 ± 0.006
3.542 ± 0.008
6.077 ± 0.019
3.599 ± 0.009
5.959 ± 0.013
8.763 ± 0.02
6.103 ± 0.012
6.477 ± 0.012
1.587 ± 0.007
2.909 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here