Pleionea mediterranea

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Pleioneaceae; Pleionea

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3566 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A316G0R5|A0A316G0R5_9GAMM UDP-N-acetylmuramoylalanine--D-glutamate ligase OS=Pleionea mediterranea OX=523701 GN=murD PE=3 SV=1
MM1 pKa = 6.82MRR3 pKa = 11.84WSIILKK9 pKa = 10.07SALILLLSIYY19 pKa = 10.93SMAAQSVIIFSDD31 pKa = 3.63DD32 pKa = 3.5FEE34 pKa = 4.67TLLTQWTVNSSGGDD48 pKa = 3.21ASIGNEE54 pKa = 3.86TSNSGNNSLRR64 pKa = 11.84LRR66 pKa = 11.84WDD68 pKa = 3.44TVTITSNTINANVSAAQVRR87 pKa = 11.84IWVRR91 pKa = 11.84RR92 pKa = 11.84GDD94 pKa = 3.96DD95 pKa = 3.46NFSEE99 pKa = 4.25NPEE102 pKa = 3.99NGEE105 pKa = 4.01NLTLQYY111 pKa = 11.33LNNSNNWITLDD122 pKa = 3.5TFFGGGSPGDD132 pKa = 3.7IFDD135 pKa = 5.17LGYY138 pKa = 11.02NLPADD143 pKa = 4.23ALHH146 pKa = 6.37SGLRR150 pKa = 11.84LRR152 pKa = 11.84LNLNTGSGNDD162 pKa = 3.14FDD164 pKa = 4.37YY165 pKa = 10.82WHH167 pKa = 6.96VDD169 pKa = 3.3DD170 pKa = 5.61VVVDD174 pKa = 4.91SIDD177 pKa = 3.31ATAPTGLVAEE187 pKa = 4.32WRR189 pKa = 11.84FDD191 pKa = 3.49EE192 pKa = 4.82LTWNSQALEE201 pKa = 4.12VRR203 pKa = 11.84DD204 pKa = 3.74SSGNFYY210 pKa = 10.74HH211 pKa = 7.41GIAFNSQPTTGFVCNAADD229 pKa = 4.06FTAAGTDD236 pKa = 3.32DD237 pKa = 4.59YY238 pKa = 11.67IRR240 pKa = 11.84LNHH243 pKa = 6.48EE244 pKa = 4.43SLNGLDD250 pKa = 4.04DD251 pKa = 3.62WSISVWVTSNRR262 pKa = 11.84NAAQTILSASKK273 pKa = 11.13NEE275 pKa = 4.15TEE277 pKa = 4.09LNEE280 pKa = 3.64AVMYY284 pKa = 10.83FNDD287 pKa = 5.22ASTFWPSVRR296 pKa = 11.84SNPFFDD302 pKa = 5.37NNTQLALPYY311 pKa = 10.48AIDD314 pKa = 4.78DD315 pKa = 4.39GNFHH319 pKa = 6.53HH320 pKa = 7.01LVWTRR325 pKa = 11.84DD326 pKa = 3.27RR327 pKa = 11.84ANSEE331 pKa = 3.79TCLYY335 pKa = 10.34LDD337 pKa = 4.28SNIVGCTTHH346 pKa = 7.04TDD348 pKa = 3.13GGNTMDD354 pKa = 4.45VIVDD358 pKa = 3.59GLVIGQEE365 pKa = 3.82QDD367 pKa = 3.45VVGGDD372 pKa = 3.5FDD374 pKa = 5.44INQVWVGLIDD384 pKa = 4.0EE385 pKa = 5.27LVLFDD390 pKa = 5.08NEE392 pKa = 4.16LSPGEE397 pKa = 4.0VLFIYY402 pKa = 10.58NNQTAGRR409 pKa = 11.84GWDD412 pKa = 3.47GSDD415 pKa = 3.31RR416 pKa = 11.84VCPEE420 pKa = 4.11ALPPTAAYY428 pKa = 9.22WRR430 pKa = 11.84MDD432 pKa = 3.09EE433 pKa = 4.43TTWDD437 pKa = 3.71GSPGEE442 pKa = 4.43VIDD445 pKa = 5.61LSGNGLNMTSYY456 pKa = 11.29GGASTNNIDD465 pKa = 3.63PAIVGDD471 pKa = 4.24PGTCGFGEE479 pKa = 4.32FDD481 pKa = 3.7GSTTYY486 pKa = 11.18LEE488 pKa = 4.57IDD490 pKa = 3.94HH491 pKa = 7.46DD492 pKa = 4.5PLLSFSTNMSITLWVKK508 pKa = 10.39PNSLPLSGLHH518 pKa = 6.36SVLSKK523 pKa = 11.04DD524 pKa = 3.47EE525 pKa = 4.3NYY527 pKa = 10.33EE528 pKa = 3.94FHH530 pKa = 6.8LTPTNEE536 pKa = 3.92IFWWWNQGALTTSGANIQPGNWYY559 pKa = 10.12HH560 pKa = 6.81IGLTFRR566 pKa = 11.84SGEE569 pKa = 3.8QIIYY573 pKa = 10.4VNGIEE578 pKa = 5.25RR579 pKa = 11.84GRR581 pKa = 11.84QNFNGTLPQNTDD593 pKa = 3.39PLQIGQDD600 pKa = 3.32QLAAGRR606 pKa = 11.84FLDD609 pKa = 4.18GQVDD613 pKa = 3.94EE614 pKa = 5.31VKK616 pKa = 10.35IFPSTLSHH624 pKa = 5.77GQVIQSMLEE633 pKa = 3.79VHH635 pKa = 7.04PCTSSSTCPYY645 pKa = 10.59DD646 pKa = 3.39YY647 pKa = 10.83TDD649 pKa = 3.49NFSVQSYY656 pKa = 10.15GNNDD660 pKa = 2.88GSNNFSGNWQEE671 pKa = 4.31TGDD674 pKa = 4.02NNSPASGSVGIAGGEE689 pKa = 3.99LYY691 pKa = 9.46LTNQNNNEE699 pKa = 3.94PSISRR704 pKa = 11.84SMDD707 pKa = 3.36LSQALTAEE715 pKa = 4.57LRR717 pKa = 11.84FEE719 pKa = 4.1MRR721 pKa = 11.84TSNTLEE727 pKa = 3.96NGDD730 pKa = 3.42RR731 pKa = 11.84VAIEE735 pKa = 3.95VSADD739 pKa = 3.08GTNYY743 pKa = 10.33TEE745 pKa = 4.15LQEE748 pKa = 4.16FRR750 pKa = 11.84NDD752 pKa = 3.12VVGVFTYY759 pKa = 10.62DD760 pKa = 2.74IAGFISANTSIRR772 pKa = 11.84FRR774 pKa = 11.84ITQGYY779 pKa = 8.01RR780 pKa = 11.84QNDD783 pKa = 3.74EE784 pKa = 3.96YY785 pKa = 11.7VYY787 pKa = 10.95FDD789 pKa = 4.14DD790 pKa = 3.9VQVNITEE797 pKa = 4.14FCGIGFFVINHH808 pKa = 6.98DD809 pKa = 3.75GSGINCLRR817 pKa = 11.84EE818 pKa = 4.08AVSITAYY825 pKa = 9.36DD826 pKa = 3.21TGGNLLTGYY835 pKa = 10.15NGLVSLSSNTSNGNWFNTDD854 pKa = 2.59SSGNSADD861 pKa = 4.28LSQGTLNDD869 pKa = 3.51TAGDD873 pKa = 3.97DD874 pKa = 3.89NGAATYY880 pKa = 10.07QFSPADD886 pKa = 3.38QGTVTLYY893 pKa = 11.16LEE895 pKa = 4.45NTHH898 pKa = 6.09QEE900 pKa = 4.44SINVAVTEE908 pKa = 4.3GAAADD913 pKa = 4.48DD914 pKa = 3.89NSEE917 pKa = 3.99GNIVFRR923 pKa = 11.84PFGFVATPSSIPTQVAGRR941 pKa = 11.84PFNLQLAAAGQTPSQPEE958 pKa = 3.92CGVIEE963 pKa = 4.81EE964 pKa = 4.34YY965 pKa = 10.32TGNKK969 pKa = 9.43DD970 pKa = 2.79IHH972 pKa = 6.11FWSSYY977 pKa = 6.98TLPNSSPTPVTINGVNVAGSEE998 pKa = 4.23PAASSQPITFNNGIADD1014 pKa = 3.64IPVQYY1019 pKa = 10.97NDD1021 pKa = 2.86VGQIQFFAKK1030 pKa = 10.43DD1031 pKa = 3.31EE1032 pKa = 4.27TGIGDD1037 pKa = 3.98PTTGNTDD1044 pKa = 3.76EE1045 pKa = 5.31IIGGIPPFVVRR1056 pKa = 11.84PFGYY1060 pKa = 10.41DD1061 pKa = 2.52ISAQGNPDD1069 pKa = 3.2ATGGASPVYY1078 pKa = 10.08AVAGNAFNMTLRR1090 pKa = 11.84SVLWQAADD1098 pKa = 3.31SDD1100 pKa = 4.8ANGVPLPGADD1110 pKa = 3.76LSDD1113 pKa = 3.73NGVTPNIVNIPSSAPDD1129 pKa = 3.13IDD1131 pKa = 3.73ITLTPTAQVVTNSDD1145 pKa = 3.5GTLSDD1150 pKa = 3.3PTINYY1155 pKa = 10.26SNFSAVGSAAQGTLTQSQSWSEE1177 pKa = 4.01VGILTLNANTPDD1189 pKa = 3.7FMATGVGVTGSKK1201 pKa = 10.8NNIGRR1206 pKa = 11.84FIPSYY1211 pKa = 10.69FSVQVGDD1218 pKa = 4.05NFEE1221 pKa = 4.21AQCTGFSYY1229 pKa = 10.85SGFNNGSAGLTKK1241 pKa = 9.98IGQSDD1246 pKa = 4.09NLTIDD1251 pKa = 3.15IQAFNANDD1259 pKa = 3.78EE1260 pKa = 4.41TTQNYY1265 pKa = 9.57DD1266 pKa = 2.71ADD1268 pKa = 4.11FAKK1271 pKa = 10.74LEE1273 pKa = 4.35SGTISTSGYY1282 pKa = 10.63DD1283 pKa = 3.5EE1284 pKa = 4.5TNAAPAPGNIVTGTVGTPVFDD1305 pKa = 3.76TDD1307 pKa = 4.13GFAQNVSIPNVYY1319 pKa = 9.37YY1320 pKa = 10.68QYY1322 pKa = 11.67DD1323 pKa = 3.47NFAAPFEE1330 pKa = 4.03LRR1332 pKa = 11.84IDD1334 pKa = 3.63IDD1336 pKa = 3.59ATDD1339 pKa = 3.29VDD1341 pKa = 4.83GVNGTASSNSVEE1353 pKa = 3.98QRR1355 pKa = 11.84LGRR1358 pKa = 11.84SRR1360 pKa = 11.84LVTAYY1365 pKa = 10.0GPEE1368 pKa = 4.05VEE1370 pKa = 4.82PLNIPLYY1377 pKa = 10.15TEE1379 pKa = 4.68FFNGTDD1385 pKa = 3.27WVINTADD1392 pKa = 3.37NCTSYY1397 pKa = 10.72ISSDD1401 pKa = 3.48LQFVTGTYY1409 pKa = 10.12QGDD1412 pKa = 3.87LNAGEE1417 pKa = 4.26TSITLPASNTLLTAGEE1433 pKa = 4.3SGVNQGFGLTAPGVGNSGEE1452 pKa = 4.28VNVEE1456 pKa = 4.17HH1457 pKa = 6.79NLSALDD1463 pKa = 3.44WLRR1466 pKa = 11.84FDD1468 pKa = 4.14WNGDD1472 pKa = 3.3NSNDD1476 pKa = 3.69NPQATAGFGRR1486 pKa = 11.84YY1487 pKa = 9.0RR1488 pKa = 11.84GSDD1491 pKa = 2.88RR1492 pKa = 11.84VIYY1495 pKa = 9.6WRR1497 pKa = 11.84EE1498 pKa = 3.47NN1499 pKa = 3.09

Molecular weight: 161.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A316FRC0|A0A316FRC0_9GAMM Phosphoserine aminotransferase OS=Pleionea mediterranea OX=523701 GN=serC PE=3 SV=1
MM1 pKa = 7.93ISITSLYY8 pKa = 10.97ASLCGILILALAIRR22 pKa = 11.84VVYY25 pKa = 10.3LRR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 8.86NKK31 pKa = 9.94VGIGNGGVDD40 pKa = 3.78DD41 pKa = 4.21LRR43 pKa = 11.84RR44 pKa = 11.84AVRR47 pKa = 11.84VHH49 pKa = 6.59ANAIEE54 pKa = 4.19YY55 pKa = 10.25IPIVLILMAALEE67 pKa = 4.35LNGVNGWLMHH77 pKa = 5.51VLGSLLVLSRR87 pKa = 11.84LLHH90 pKa = 5.47AQGLSGSPGVSFGRR104 pKa = 11.84FWGTLVTWIVIIISVVANLVQFFANN129 pKa = 3.52

Molecular weight: 13.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3566

0

3566

1176721

25

2691

330.0

36.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.819 ± 0.046

0.938 ± 0.013

5.912 ± 0.053

6.201 ± 0.047

4.256 ± 0.031

6.361 ± 0.046

2.227 ± 0.021

6.49 ± 0.033

5.793 ± 0.046

10.146 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.026

4.852 ± 0.038

3.782 ± 0.023

4.958 ± 0.04

4.397 ± 0.031

7.32 ± 0.042

5.29 ± 0.033

6.519 ± 0.038

1.259 ± 0.016

3.125 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski