Ornithinimicrobium sp. AMA3305
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3428 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345NQA4|A0A345NQA4_9MICO Glutamyl-tRNA reductase OS=Ornithinimicrobium sp. AMA3305 OX=2283195 GN=hemA PE=3 SV=1
MM1 pKa = 7.64 GGRR4 pKa = 11.84 HH5 pKa = 5.77 ARR7 pKa = 11.84 TPRR10 pKa = 11.84 LLASLLTLTLLAGCTFAGGDD30 pKa = 3.71 GGAPTPTGTATQDD43 pKa = 3.05 GFSTDD48 pKa = 3.53 GPVTSTSTPDD58 pKa = 3.49 ADD60 pKa = 3.73 GAPAGLEE67 pKa = 3.96 DD68 pKa = 5.84 FYY70 pKa = 11.59 GQQLEE75 pKa = 4.44 WTDD78 pKa = 3.79 CEE80 pKa = 5.12 SLFEE84 pKa = 4.24 CANLEE89 pKa = 4.26 VPVDD93 pKa = 3.88 YY94 pKa = 10.32 EE95 pKa = 4.49 DD96 pKa = 4.13 PGGPTIEE103 pKa = 4.37 LAVLRR108 pKa = 11.84 AGASGEE114 pKa = 4.14 PQGSLVVNPGGPGASGVDD132 pKa = 3.6 YY133 pKa = 11.61 AMMAGAVVSRR143 pKa = 11.84 EE144 pKa = 3.4 VRR146 pKa = 11.84 AAYY149 pKa = 9.95 DD150 pKa = 3.35 VVGFDD155 pKa = 3.51 PRR157 pKa = 11.84 GVARR161 pKa = 11.84 SAPITCFTDD170 pKa = 3.21 EE171 pKa = 5.03 QMDD174 pKa = 4.1 TFLGTDD180 pKa = 4.47 PSPDD184 pKa = 3.41 DD185 pKa = 3.84 PAEE188 pKa = 3.94 EE189 pKa = 3.79 RR190 pKa = 11.84 AVEE193 pKa = 4.36 GEE195 pKa = 4.36 LKK197 pKa = 10.82 DD198 pKa = 3.71 FAQACEE204 pKa = 4.21 TNAGDD209 pKa = 4.51 LLGHH213 pKa = 6.23 VSTVEE218 pKa = 3.53 AAKK221 pKa = 11.19 DD222 pKa = 3.53 MDD224 pKa = 4.16 VLRR227 pKa = 11.84 AALGEE232 pKa = 3.93 PRR234 pKa = 11.84 LTYY237 pKa = 10.74 LGASYY242 pKa = 8.37 GTFLGTTYY250 pKa = 10.29 ATLFPDD256 pKa = 3.18 RR257 pKa = 11.84 VGRR260 pKa = 11.84 LVLDD264 pKa = 4.16 GAIDD268 pKa = 3.63 PTLTGLEE275 pKa = 4.12 MGLGQAAGFEE285 pKa = 4.47 RR286 pKa = 11.84 ATRR289 pKa = 11.84 AYY291 pKa = 10.72 VEE293 pKa = 4.19 NCVGGGSCPLGDD305 pKa = 4.8 DD306 pKa = 3.44 VDD308 pKa = 4.09 TAMVAIPAFLDD319 pKa = 3.59 EE320 pKa = 4.74 VDD322 pKa = 3.68 AHH324 pKa = 6.55 PLPVSGDD331 pKa = 3.42 TVTEE335 pKa = 4.15 LTEE338 pKa = 3.88 GWAMLGIIVAMYY350 pKa = 10.26 SQDD353 pKa = 2.49 IWPILTQALGRR364 pKa = 11.84 AQQGDD369 pKa = 3.83 GTLLMFLANSYY380 pKa = 11.07 ASRR383 pKa = 11.84 HH384 pKa = 5.28 SDD386 pKa = 2.72 GTYY389 pKa = 9.75 TGNGMQAIYY398 pKa = 10.47 AVNCLDD404 pKa = 3.73 RR405 pKa = 11.84 PADD408 pKa = 3.93 EE409 pKa = 6.21 GPDD412 pKa = 3.5 LDD414 pKa = 3.94 SAAVEE419 pKa = 4.31 EE420 pKa = 4.37 QFEE423 pKa = 4.4 AASPTWGRR431 pKa = 11.84 YY432 pKa = 7.51 LAGDD436 pKa = 4.56 GACEE440 pKa = 3.91 YY441 pKa = 9.96 WPVHH445 pKa = 6.52 AEE447 pKa = 3.8 QTLQDD452 pKa = 3.94 YY453 pKa = 9.64 SAQGAAPIVVIGTTRR468 pKa = 11.84 DD469 pKa = 3.08 PATPYY474 pKa = 10.19 EE475 pKa = 4.15 WAEE478 pKa = 3.9 ALADD482 pKa = 3.72 ILDD485 pKa = 4.04 SGVLISYY492 pKa = 10.54 DD493 pKa = 3.73 GDD495 pKa = 3.21 GHH497 pKa = 5.16 TAYY500 pKa = 10.16 GRR502 pKa = 11.84 SNDD505 pKa = 3.98 CVDD508 pKa = 4.3 DD509 pKa = 4.84 AVDD512 pKa = 3.93 AYY514 pKa = 10.93 LLEE517 pKa = 4.55 GTVPQDD523 pKa = 3.9 GLRR526 pKa = 11.84 CC527 pKa = 3.91
Molecular weight: 54.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.605
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.656
Grimsley 3.516
Solomon 3.821
Lehninger 3.77
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.656
EMBOSS 3.757
Sillero 3.948
Patrickios 0.795
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A345NP23|A0A345NP23_9MICO DUF721 domain-containing protein OS=Ornithinimicrobium sp. AMA3305 OX=2283195 GN=DV701_12205 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 ARR15 pKa = 11.84 KK16 pKa = 8.65 HH17 pKa = 4.47 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.65 GRR40 pKa = 11.84 SALSAA45 pKa = 3.8
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.705
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.427
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.265
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3428
0
3428
1146953
32
3005
334.6
35.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.048 ± 0.053
0.655 ± 0.01
6.256 ± 0.041
5.922 ± 0.038
2.472 ± 0.026
9.636 ± 0.035
2.31 ± 0.021
2.861 ± 0.035
1.497 ± 0.026
10.656 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.867 ± 0.019
1.412 ± 0.021
5.823 ± 0.033
3.048 ± 0.022
8.12 ± 0.052
5.0 ± 0.026
6.044 ± 0.029
9.924 ± 0.043
1.605 ± 0.019
1.843 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here