Seonamhaeicola algicola
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3062 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C7AGT7|A0A5C7AGT7_9FLAO UnbV_ASPIC domain-containing protein OS=Seonamhaeicola algicola OX=1719036 GN=FUA26_13400 PE=4 SV=1
MM1 pKa = 7.37 KK2 pKa = 10.39 LEE4 pKa = 4.97 KK5 pKa = 10.05 ISLLIFCVALCMFSCGNDD23 pKa = 3.38 DD24 pKa = 5.79 DD25 pKa = 4.48 GTTDD29 pKa = 3.28 VEE31 pKa = 4.11 IVIRR35 pKa = 11.84 DD36 pKa = 3.53 RR37 pKa = 11.84 NEE39 pKa = 3.52 QQVIDD44 pKa = 4.07 NNSLIEE50 pKa = 3.97 FLEE53 pKa = 4.0 THH55 pKa = 6.72 YY56 pKa = 11.3 YY57 pKa = 9.96 NSDD60 pKa = 3.26 VFEE63 pKa = 4.87 NNANASISDD72 pKa = 4.0 LVILPLATGEE82 pKa = 4.38 TVPNGYY88 pKa = 7.77 TLLKK92 pKa = 10.53 DD93 pKa = 3.46 AVGVSRR99 pKa = 11.84 KK100 pKa = 6.64 TTFADD105 pKa = 2.92 TDD107 pKa = 3.86 YY108 pKa = 11.07 EE109 pKa = 4.61 FYY111 pKa = 11.51 VLVLNEE117 pKa = 4.18 GGGDD121 pKa = 4.22 FKK123 pKa = 11.27 PSFADD128 pKa = 4.35 NILTTYY134 pKa = 9.94 QGYY137 pKa = 7.76 TLDD140 pKa = 5.59 DD141 pKa = 4.13 NLNDD145 pKa = 3.24 ITDD148 pKa = 4.02 AFDD151 pKa = 4.37 SKK153 pKa = 8.47 TTPDD157 pKa = 3.44 TYY159 pKa = 10.94 FGLIDD164 pKa = 6.4 LIPAWRR170 pKa = 11.84 RR171 pKa = 11.84 VFPMFNVANGYY182 pKa = 9.41 VDD184 pKa = 4.57 NGDD187 pKa = 3.26 GTVNFNNPGMGVMFIPSGLAYY208 pKa = 10.0 FQEE211 pKa = 4.35 ARR213 pKa = 11.84 TDD215 pKa = 3.71 LPSYY219 pKa = 10.65 SPLIFKK225 pKa = 10.62 FEE227 pKa = 4.98 LIHH230 pKa = 8.0 AIEE233 pKa = 5.22 LDD235 pKa = 3.42 HH236 pKa = 7.91 DD237 pKa = 4.72 GDD239 pKa = 4.97 GIPSYY244 pKa = 11.17 LEE246 pKa = 3.85 EE247 pKa = 4.67 LSGNQEE253 pKa = 3.96 FNINSEE259 pKa = 4.13 AGVFDD264 pKa = 5.85 GDD266 pKa = 3.95 DD267 pKa = 3.55 TDD269 pKa = 4.5 EE270 pKa = 4.37 NSIPNYY276 pKa = 10.23 FDD278 pKa = 3.62 TDD280 pKa = 3.8 DD281 pKa = 5.45 DD282 pKa = 5.13 GDD284 pKa = 4.25 GVSTATEE291 pKa = 3.49 ISEE294 pKa = 3.93 LFEE297 pKa = 3.87 IKK299 pKa = 10.18 KK300 pKa = 10.6 YY301 pKa = 10.89 NEE303 pKa = 3.62 PTKK306 pKa = 10.72 AALEE310 pKa = 4.25 ALTFAANEE318 pKa = 4.09 KK319 pKa = 9.88 LVHH322 pKa = 6.37 IVEE325 pKa = 4.57 LANGTFTGTTITAADD340 pKa = 3.84 TDD342 pKa = 3.86 GDD344 pKa = 4.63 GIFDD348 pKa = 4.44 YY349 pKa = 11.39 LDD351 pKa = 3.8 ADD353 pKa = 3.75
Molecular weight: 38.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.49
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.075
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.773
Protein with the highest isoelectric point:
>tr|A0A5C7AN91|A0A5C7AN91_9FLAO 50S ribosomal protein L18 OS=Seonamhaeicola algicola OX=1719036 GN=rplR PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.19 RR22 pKa = 11.84 MATANGRR29 pKa = 11.84 KK30 pKa = 8.93 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 7.97 KK42 pKa = 10.6 LSVSSEE48 pKa = 4.04 SRR50 pKa = 11.84 HH51 pKa = 5.63 KK52 pKa = 10.69 KK53 pKa = 9.55
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3062
0
3062
1125401
38
4371
367.5
41.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.613 ± 0.038
0.759 ± 0.014
5.311 ± 0.037
6.05 ± 0.041
5.218 ± 0.031
6.224 ± 0.049
1.87 ± 0.025
7.784 ± 0.042
8.032 ± 0.074
9.014 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.984 ± 0.02
7.38 ± 0.061
3.385 ± 0.024
3.295 ± 0.023
3.073 ± 0.027
6.088 ± 0.034
6.39 ± 0.067
6.312 ± 0.03
1.094 ± 0.018
4.125 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here