Streptococcus phage SMP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1EAB5|A1EAB5_9CAUD Putative holin OS=Streptococcus phage SMP OX=413066 PE=4 SV=1
MM1 pKa = 7.16TQTTDD6 pKa = 2.97NTLLNLEE13 pKa = 4.59EE14 pKa = 4.27TTQPFDD20 pKa = 3.26LATALTYY27 pKa = 9.97MKK29 pKa = 10.29EE30 pKa = 3.53HH31 pKa = 7.09GEE33 pKa = 4.26FIRR36 pKa = 11.84CKK38 pKa = 10.3SANQDD43 pKa = 2.65FYY45 pKa = 11.15MYY47 pKa = 10.56RR48 pKa = 11.84DD49 pKa = 3.66VQKK52 pKa = 10.79RR53 pKa = 11.84PAIVNGRR60 pKa = 11.84RR61 pKa = 11.84KK62 pKa = 9.67FVDD65 pKa = 3.7VEE67 pKa = 4.51TIWAFNQWGGTAATINIADD86 pKa = 4.34MLNEE90 pKa = 4.8EE91 pKa = 4.2YY92 pKa = 10.01WIMKK96 pKa = 9.71FDD98 pKa = 4.32EE99 pKa = 4.89NGNPDD104 pKa = 3.35WTDD107 pKa = 3.35PTAGAEE113 pKa = 4.08AA114 pKa = 4.24

Molecular weight: 13.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1EAE7|A1EAE7_9CAUD Uncharacterized protein OS=Streptococcus phage SMP OX=413066 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 9.97RR4 pKa = 11.84IKK6 pKa = 10.48KK7 pKa = 7.44KK8 pKa = 7.6WSRR11 pKa = 11.84IEE13 pKa = 3.82RR14 pKa = 11.84LEE16 pKa = 4.08NKK18 pKa = 9.0VAQLTAEE25 pKa = 4.68NILLTDD31 pKa = 4.19ALRR34 pKa = 11.84NHH36 pKa = 7.35ADD38 pKa = 3.63NIRR41 pKa = 11.84DD42 pKa = 3.46LDD44 pKa = 4.82DD45 pKa = 3.43IVKK48 pKa = 10.63RR49 pKa = 11.84NAQATNSRR57 pKa = 11.84FDD59 pKa = 3.46KK60 pKa = 11.08LEE62 pKa = 3.99KK63 pKa = 10.23QVANGNTKK71 pKa = 10.1KK72 pKa = 10.54PFWKK76 pKa = 10.34RR77 pKa = 3.03

Molecular weight: 9.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

10321

50

1161

219.6

24.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.683 ± 0.46

0.31 ± 0.08

6.472 ± 0.412

7.286 ± 0.423

3.827 ± 0.228

6.288 ± 0.47

1.502 ± 0.165

6.966 ± 0.33

8.274 ± 0.489

7.751 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.635 ± 0.219

5.591 ± 0.307

2.742 ± 0.238

4.118 ± 0.261

4.447 ± 0.358

5.746 ± 0.283

6.298 ± 0.331

6.608 ± 0.381

1.589 ± 0.231

3.866 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski