Amino acid dipepetide frequency for Streptococcus phage SMP

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.942AlaAla: 4.942 ± 0.916
0.388AlaCys: 0.388 ± 0.173
5.426AlaAsp: 5.426 ± 0.672
6.589AlaGlu: 6.589 ± 1.127
2.519AlaPhe: 2.519 ± 0.456
5.814AlaGly: 5.814 ± 1.052
1.163AlaHis: 1.163 ± 0.299
6.492AlaIle: 6.492 ± 1.43
5.911AlaLys: 5.911 ± 0.719
5.814AlaLeu: 5.814 ± 0.632
1.453AlaMet: 1.453 ± 0.337
4.07AlaAsn: 4.07 ± 0.598
1.55AlaPro: 1.55 ± 0.359
3.198AlaGln: 3.198 ± 0.686
3.876AlaArg: 3.876 ± 0.877
4.651AlaSer: 4.651 ± 0.685
3.682AlaThr: 3.682 ± 0.682
5.329AlaVal: 5.329 ± 1.181
1.26AlaTrp: 1.26 ± 0.751
2.713AlaTyr: 2.713 ± 0.551
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.484CysGlu: 0.484 ± 0.193
0.194CysPhe: 0.194 ± 0.136
0.291CysGly: 0.291 ± 0.171
0.097CysHis: 0.097 ± 0.101
0.097CysIle: 0.097 ± 0.082
0.291CysLys: 0.291 ± 0.176
0.291CysLeu: 0.291 ± 0.184
0.0CysMet: 0.0 ± 0.0
0.097CysAsn: 0.097 ± 0.094
0.097CysPro: 0.097 ± 0.101
0.194CysGln: 0.194 ± 0.123
0.194CysArg: 0.194 ± 0.123
0.484CysSer: 0.484 ± 0.192
0.0CysThr: 0.0 ± 0.0
0.097CysVal: 0.097 ± 0.089
0.097CysTrp: 0.097 ± 0.101
0.097CysTyr: 0.097 ± 0.094
0.0CysXaa: 0.0 ± 0.0
Asp
4.748AspAla: 4.748 ± 0.789
0.388AspCys: 0.388 ± 0.186
3.198AspAsp: 3.198 ± 0.629
3.876AspGlu: 3.876 ± 0.655
3.585AspPhe: 3.585 ± 0.629
4.36AspGly: 4.36 ± 0.703
0.581AspHis: 0.581 ± 0.201
5.233AspIle: 5.233 ± 0.511
6.298AspLys: 6.298 ± 0.611
5.233AspLeu: 5.233 ± 0.497
1.744AspMet: 1.744 ± 0.395
3.876AspAsn: 3.876 ± 0.708
1.841AspPro: 1.841 ± 0.355
1.26AspGln: 1.26 ± 0.304
2.616AspArg: 2.616 ± 0.499
2.81AspSer: 2.81 ± 0.63
3.391AspThr: 3.391 ± 0.506
4.36AspVal: 4.36 ± 0.743
1.841AspTrp: 1.841 ± 0.427
3.488AspTyr: 3.488 ± 0.871
0.0AspXaa: 0.0 ± 0.0
Glu
6.202GluAla: 6.202 ± 0.785
0.0GluCys: 0.0 ± 0.0
4.264GluAsp: 4.264 ± 0.683
5.039GluGlu: 5.039 ± 0.659
2.035GluPhe: 2.035 ± 0.449
4.07GluGly: 4.07 ± 0.657
1.938GluHis: 1.938 ± 0.401
6.686GluIle: 6.686 ± 0.8
4.845GluLys: 4.845 ± 1.062
5.911GluLeu: 5.911 ± 0.79
3.682GluMet: 3.682 ± 0.57
4.167GluAsn: 4.167 ± 0.605
1.938GluPro: 1.938 ± 0.602
2.907GluGln: 2.907 ± 0.476
3.004GluArg: 3.004 ± 0.563
3.295GluSer: 3.295 ± 0.548
4.457GluThr: 4.457 ± 0.651
4.845GluVal: 4.845 ± 0.867
1.26GluTrp: 1.26 ± 0.257
2.326GluTyr: 2.326 ± 0.479
0.0GluXaa: 0.0 ± 0.0
Phe
3.198PheAla: 3.198 ± 0.491
0.0PheCys: 0.0 ± 0.0
3.973PheAsp: 3.973 ± 0.687
3.488PheGlu: 3.488 ± 0.639
0.969PhePhe: 0.969 ± 0.274
2.035PheGly: 2.035 ± 0.447
0.291PheHis: 0.291 ± 0.132
1.55PheIle: 1.55 ± 0.389
2.326PheLys: 2.326 ± 0.442
2.81PheLeu: 2.81 ± 0.715
0.872PheMet: 0.872 ± 0.369
1.938PheAsn: 1.938 ± 0.421
0.581PhePro: 0.581 ± 0.274
1.357PheGln: 1.357 ± 0.474
1.938PheArg: 1.938 ± 0.553
2.81PheSer: 2.81 ± 0.556
2.616PheThr: 2.616 ± 0.492
3.101PheVal: 3.101 ± 0.614
0.388PheTrp: 0.388 ± 0.177
2.035PheTyr: 2.035 ± 0.508
0.0PheXaa: 0.0 ± 0.0
Gly
3.488GlyAla: 3.488 ± 0.928
0.194GlyCys: 0.194 ± 0.142
3.682GlyAsp: 3.682 ± 0.499
4.167GlyGlu: 4.167 ± 0.557
2.616GlyPhe: 2.616 ± 0.542
3.973GlyGly: 3.973 ± 0.92
1.55GlyHis: 1.55 ± 0.379
4.942GlyIle: 4.942 ± 0.86
5.62GlyLys: 5.62 ± 1.008
4.845GlyLeu: 4.845 ± 0.822
1.841GlyMet: 1.841 ± 0.524
2.422GlyAsn: 2.422 ± 0.495
1.26GlyPro: 1.26 ± 0.423
3.295GlyGln: 3.295 ± 0.509
2.713GlyArg: 2.713 ± 0.442
3.876GlySer: 3.876 ± 0.49
3.779GlyThr: 3.779 ± 0.516
4.942GlyVal: 4.942 ± 0.539
0.484GlyTrp: 0.484 ± 0.221
3.101GlyTyr: 3.101 ± 0.475
0.0GlyXaa: 0.0 ± 0.0
His
0.969HisAla: 0.969 ± 0.274
0.194HisCys: 0.194 ± 0.132
1.55HisAsp: 1.55 ± 0.373
0.872HisGlu: 0.872 ± 0.317
0.678HisPhe: 0.678 ± 0.268
0.969HisGly: 0.969 ± 0.337
0.0HisHis: 0.0 ± 0.0
1.357HisIle: 1.357 ± 0.376
1.163HisLys: 1.163 ± 0.301
1.453HisLeu: 1.453 ± 0.316
0.388HisMet: 0.388 ± 0.199
0.678HisAsn: 0.678 ± 0.212
0.872HisPro: 0.872 ± 0.262
0.291HisGln: 0.291 ± 0.218
0.775HisArg: 0.775 ± 0.345
0.872HisSer: 0.872 ± 0.262
0.678HisThr: 0.678 ± 0.262
0.291HisVal: 0.291 ± 0.147
0.097HisTrp: 0.097 ± 0.082
0.872HisTyr: 0.872 ± 0.335
0.0HisXaa: 0.0 ± 0.0
Ile
4.942IleAla: 4.942 ± 0.669
0.194IleCys: 0.194 ± 0.146
4.264IleAsp: 4.264 ± 0.776
5.136IleGlu: 5.136 ± 0.751
2.229IlePhe: 2.229 ± 0.317
4.07IleGly: 4.07 ± 0.669
0.969IleHis: 0.969 ± 0.261
3.585IleIle: 3.585 ± 0.595
7.655IleLys: 7.655 ± 1.237
5.329IleLeu: 5.329 ± 0.596
1.357IleMet: 1.357 ± 0.409
3.973IleAsn: 3.973 ± 0.718
1.938IlePro: 1.938 ± 0.414
2.519IleGln: 2.519 ± 0.513
3.198IleArg: 3.198 ± 0.477
4.457IleSer: 4.457 ± 1.179
4.748IleThr: 4.748 ± 0.701
4.07IleVal: 4.07 ± 0.742
1.841IleTrp: 1.841 ± 0.714
3.198IleTyr: 3.198 ± 0.65
0.0IleXaa: 0.0 ± 0.0
Lys
7.849LysAla: 7.849 ± 0.747
0.194LysCys: 0.194 ± 0.145
5.233LysAsp: 5.233 ± 0.797
5.426LysGlu: 5.426 ± 1.047
2.81LysPhe: 2.81 ± 0.512
4.748LysGly: 4.748 ± 0.793
0.969LysHis: 0.969 ± 0.393
4.942LysIle: 4.942 ± 0.829
7.461LysLys: 7.461 ± 1.205
4.651LysLeu: 4.651 ± 0.67
2.713LysMet: 2.713 ± 0.684
4.457LysAsn: 4.457 ± 0.662
3.391LysPro: 3.391 ± 0.564
4.554LysGln: 4.554 ± 0.817
3.779LysArg: 3.779 ± 0.774
4.457LysSer: 4.457 ± 0.736
6.008LysThr: 6.008 ± 0.719
4.845LysVal: 4.845 ± 0.653
1.26LysTrp: 1.26 ± 0.361
3.004LysTyr: 3.004 ± 0.51
0.0LysXaa: 0.0 ± 0.0
Leu
6.202LeuAla: 6.202 ± 0.651
0.291LeuCys: 0.291 ± 0.183
5.136LeuAsp: 5.136 ± 0.7
6.202LeuGlu: 6.202 ± 0.945
3.101LeuPhe: 3.101 ± 0.679
4.167LeuGly: 4.167 ± 0.905
0.969LeuHis: 0.969 ± 0.299
4.748LeuIle: 4.748 ± 0.872
7.461LeuLys: 7.461 ± 0.947
6.589LeuLeu: 6.589 ± 1.003
1.357LeuMet: 1.357 ± 0.313
4.845LeuAsn: 4.845 ± 0.641
2.81LeuPro: 2.81 ± 0.449
2.907LeuGln: 2.907 ± 0.479
3.295LeuArg: 3.295 ± 0.585
3.876LeuSer: 3.876 ± 0.62
6.008LeuThr: 6.008 ± 0.716
5.039LeuVal: 5.039 ± 0.709
0.969LeuTrp: 0.969 ± 0.276
1.55LeuTyr: 1.55 ± 0.397
0.0LeuXaa: 0.0 ± 0.0
Met
2.132MetAla: 2.132 ± 0.483
0.097MetCys: 0.097 ± 0.098
1.55MetAsp: 1.55 ± 0.321
2.519MetGlu: 2.519 ± 0.567
1.163MetPhe: 1.163 ± 0.285
0.775MetGly: 0.775 ± 0.253
0.678MetHis: 0.678 ± 0.243
2.035MetIle: 2.035 ± 0.571
2.132MetLys: 2.132 ± 0.499
2.422MetLeu: 2.422 ± 0.513
0.581MetMet: 0.581 ± 0.298
1.26MetAsn: 1.26 ± 0.374
0.388MetPro: 0.388 ± 0.274
1.066MetGln: 1.066 ± 0.317
1.066MetArg: 1.066 ± 0.289
1.55MetSer: 1.55 ± 0.416
2.907MetThr: 2.907 ± 0.418
1.357MetVal: 1.357 ± 0.319
0.291MetTrp: 0.291 ± 0.17
0.388MetTyr: 0.388 ± 0.21
0.0MetXaa: 0.0 ± 0.0
Asn
4.748AsnAla: 4.748 ± 0.987
0.388AsnCys: 0.388 ± 0.172
2.616AsnAsp: 2.616 ± 0.692
2.907AsnGlu: 2.907 ± 0.494
2.035AsnPhe: 2.035 ± 0.378
6.008AsnGly: 6.008 ± 0.851
0.678AsnHis: 0.678 ± 0.238
3.682AsnIle: 3.682 ± 0.694
3.488AsnLys: 3.488 ± 0.651
4.748AsnLeu: 4.748 ± 0.712
1.066AsnMet: 1.066 ± 0.375
4.264AsnAsn: 4.264 ± 0.684
1.744AsnPro: 1.744 ± 0.442
3.779AsnGln: 3.779 ± 0.626
2.713AsnArg: 2.713 ± 0.653
3.295AsnSer: 3.295 ± 0.622
2.326AsnThr: 2.326 ± 0.466
2.229AsnVal: 2.229 ± 0.502
1.066AsnTrp: 1.066 ± 0.282
2.132AsnTyr: 2.132 ± 0.382
0.0AsnXaa: 0.0 ± 0.0
Pro
1.357ProAla: 1.357 ± 0.358
0.0ProCys: 0.0 ± 0.0
2.229ProAsp: 2.229 ± 0.483
2.229ProGlu: 2.229 ± 0.458
1.26ProPhe: 1.26 ± 0.393
0.775ProGly: 0.775 ± 0.263
0.678ProHis: 0.678 ± 0.35
1.938ProIle: 1.938 ± 0.419
2.519ProLys: 2.519 ± 0.577
1.26ProLeu: 1.26 ± 0.303
1.066ProMet: 1.066 ± 0.398
2.229ProAsn: 2.229 ± 0.666
0.581ProPro: 0.581 ± 0.351
0.969ProGln: 0.969 ± 0.261
1.163ProArg: 1.163 ± 0.396
2.229ProSer: 2.229 ± 0.359
1.26ProThr: 1.26 ± 0.293
2.035ProVal: 2.035 ± 0.393
0.484ProTrp: 0.484 ± 0.188
1.163ProTyr: 1.163 ± 0.408
0.0ProXaa: 0.0 ± 0.0
Gln
4.07GlnAla: 4.07 ± 0.771
0.0GlnCys: 0.0 ± 0.0
1.647GlnAsp: 1.647 ± 0.369
3.198GlnGlu: 3.198 ± 0.461
1.26GlnPhe: 1.26 ± 0.306
2.229GlnGly: 2.229 ± 0.495
0.678GlnHis: 0.678 ± 0.369
3.779GlnIle: 3.779 ± 0.545
3.198GlnLys: 3.198 ± 0.458
3.198GlnLeu: 3.198 ± 0.665
0.678GlnMet: 0.678 ± 0.242
2.713GlnAsn: 2.713 ± 0.565
1.26GlnPro: 1.26 ± 0.439
1.647GlnGln: 1.647 ± 0.483
1.647GlnArg: 1.647 ± 0.523
2.616GlnSer: 2.616 ± 0.592
3.295GlnThr: 3.295 ± 0.573
2.326GlnVal: 2.326 ± 0.548
0.194GlnTrp: 0.194 ± 0.147
1.55GlnTyr: 1.55 ± 0.3
0.0GlnXaa: 0.0 ± 0.0
Arg
2.422ArgAla: 2.422 ± 0.403
0.194ArgCys: 0.194 ± 0.135
2.326ArgAsp: 2.326 ± 0.486
2.616ArgGlu: 2.616 ± 0.678
2.132ArgPhe: 2.132 ± 0.429
2.229ArgGly: 2.229 ± 0.447
1.163ArgHis: 1.163 ± 0.297
3.973ArgIle: 3.973 ± 0.902
3.488ArgLys: 3.488 ± 0.634
4.554ArgLeu: 4.554 ± 0.587
1.163ArgMet: 1.163 ± 0.378
2.519ArgAsn: 2.519 ± 0.483
1.163ArgPro: 1.163 ± 0.387
1.841ArgGln: 1.841 ± 0.44
3.101ArgArg: 3.101 ± 0.635
1.841ArgSer: 1.841 ± 0.375
2.616ArgThr: 2.616 ± 0.461
2.616ArgVal: 2.616 ± 0.43
0.484ArgTrp: 0.484 ± 0.217
2.035ArgTyr: 2.035 ± 0.504
0.0ArgXaa: 0.0 ± 0.0
Ser
5.426SerAla: 5.426 ± 1.026
0.097SerCys: 0.097 ± 0.082
3.391SerAsp: 3.391 ± 0.603
4.264SerGlu: 4.264 ± 0.622
1.938SerPhe: 1.938 ± 0.506
4.457SerGly: 4.457 ± 0.9
0.388SerHis: 0.388 ± 0.173
4.554SerIle: 4.554 ± 0.672
4.457SerLys: 4.457 ± 0.82
4.651SerLeu: 4.651 ± 0.535
1.357SerMet: 1.357 ± 0.377
3.295SerAsn: 3.295 ± 0.785
1.357SerPro: 1.357 ± 0.329
2.035SerGln: 2.035 ± 0.419
2.422SerArg: 2.422 ± 0.42
3.295SerSer: 3.295 ± 0.67
3.004SerThr: 3.004 ± 0.779
4.264SerVal: 4.264 ± 0.658
0.388SerTrp: 0.388 ± 0.177
2.422SerTyr: 2.422 ± 0.52
0.0SerXaa: 0.0 ± 0.0
Thr
5.717ThrAla: 5.717 ± 1.155
0.0ThrCys: 0.0 ± 0.0
4.264ThrAsp: 4.264 ± 0.609
4.07ThrGlu: 4.07 ± 0.644
3.101ThrPhe: 3.101 ± 0.539
4.554ThrGly: 4.554 ± 0.673
0.969ThrHis: 0.969 ± 0.311
4.07ThrIle: 4.07 ± 0.663
4.264ThrLys: 4.264 ± 0.771
5.136ThrLeu: 5.136 ± 0.685
0.872ThrMet: 0.872 ± 0.243
2.907ThrAsn: 2.907 ± 0.683
1.647ThrPro: 1.647 ± 0.485
2.422ThrGln: 2.422 ± 0.595
1.357ThrArg: 1.357 ± 0.402
3.779ThrSer: 3.779 ± 0.388
3.876ThrThr: 3.876 ± 0.811
6.686ThrVal: 6.686 ± 1.485
0.678ThrTrp: 0.678 ± 0.29
2.616ThrTyr: 2.616 ± 0.477
0.0ThrXaa: 0.0 ± 0.0
Val
4.942ValAla: 4.942 ± 0.784
0.194ValCys: 0.194 ± 0.115
5.62ValAsp: 5.62 ± 0.579
4.845ValGlu: 4.845 ± 0.643
2.229ValPhe: 2.229 ± 0.545
3.682ValGly: 3.682 ± 0.68
0.291ValHis: 0.291 ± 0.166
3.391ValIle: 3.391 ± 0.592
5.233ValLys: 5.233 ± 0.678
4.554ValLeu: 4.554 ± 0.622
2.229ValMet: 2.229 ± 0.637
3.101ValAsn: 3.101 ± 0.437
1.55ValPro: 1.55 ± 0.337
2.326ValGln: 2.326 ± 0.515
2.519ValArg: 2.519 ± 0.491
4.942ValSer: 4.942 ± 0.78
4.748ValThr: 4.748 ± 0.748
5.329ValVal: 5.329 ± 0.869
1.938ValTrp: 1.938 ± 0.455
2.422ValTyr: 2.422 ± 0.465
0.0ValXaa: 0.0 ± 0.0
Trp
0.969TrpAla: 0.969 ± 0.307
0.097TrpCys: 0.097 ± 0.11
1.357TrpAsp: 1.357 ± 0.442
1.938TrpGlu: 1.938 ± 0.674
0.581TrpPhe: 0.581 ± 0.237
1.453TrpGly: 1.453 ± 0.389
0.194TrpHis: 0.194 ± 0.134
0.388TrpIle: 0.388 ± 0.209
1.163TrpLys: 1.163 ± 0.347
1.357TrpLeu: 1.357 ± 0.472
0.388TrpMet: 0.388 ± 0.237
1.066TrpAsn: 1.066 ± 0.588
0.097TrpPro: 0.097 ± 0.091
0.775TrpGln: 0.775 ± 0.298
0.484TrpArg: 0.484 ± 0.243
1.066TrpSer: 1.066 ± 0.277
0.969TrpThr: 0.969 ± 0.279
0.678TrpVal: 0.678 ± 0.213
0.388TrpTrp: 0.388 ± 0.172
0.484TrpTyr: 0.484 ± 0.229
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.519TyrAla: 2.519 ± 0.566
0.194TyrCys: 0.194 ± 0.11
3.004TyrAsp: 3.004 ± 0.605
3.004TyrGlu: 3.004 ± 0.553
1.841TyrPhe: 1.841 ± 0.45
2.229TyrGly: 2.229 ± 0.44
0.775TyrHis: 0.775 ± 0.28
2.229TyrIle: 2.229 ± 0.44
3.779TyrLys: 3.779 ± 0.709
2.713TyrLeu: 2.713 ± 0.585
1.453TyrMet: 1.453 ± 0.377
2.035TyrAsn: 2.035 ± 0.37
1.357TyrPro: 1.357 ± 0.422
1.841TyrGln: 1.841 ± 0.496
2.616TyrArg: 2.616 ± 0.605
1.26TyrSer: 1.26 ± 0.395
2.616TyrThr: 2.616 ± 0.47
1.647TyrVal: 1.647 ± 0.298
0.484TyrTrp: 0.484 ± 0.21
1.066TyrTyr: 1.066 ± 0.258
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (10321 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski