Mesonia sp. K7
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2375 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W1K6Y6|A0A2W1K6Y6_9FLAO DUF192 domain-containing protein OS=Mesonia sp. K7 OX=2218606 GN=DNG35_06485 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.33 KK3 pKa = 10.44 LLFFYY8 pKa = 10.56 FLLIGFYY15 pKa = 10.11 TYY17 pKa = 11.0 GQVGIGTAMPDD28 pKa = 3.27 PSAQLDD34 pKa = 3.82 VTVAAGDD41 pKa = 3.65 KK42 pKa = 10.74 GVLLPRR48 pKa = 11.84 VALINTTDD56 pKa = 3.02 QTTITNGNVEE66 pKa = 4.3 SLLVYY71 pKa = 9.2 NTATQNNVTPGYY83 pKa = 8.59 YY84 pKa = 9.55 YY85 pKa = 9.88 WYY87 pKa = 8.5 NGSWRR92 pKa = 11.84 RR93 pKa = 11.84 LTALGDD99 pKa = 3.69 HH100 pKa = 7.42 PEE102 pKa = 4.51 TITTLVNNTDD112 pKa = 2.62 GSYY115 pKa = 10.05 TYY117 pKa = 10.1 TSEE120 pKa = 4.68 DD121 pKa = 2.97 GTMTTFTVGGMMVDD135 pKa = 3.82 NGDD138 pKa = 3.26 GTYY141 pKa = 10.64 TFTNPDD147 pKa = 3.3 GTNVTIQGVSQALTILSYY165 pKa = 11.63 DD166 pKa = 3.71 NNTGMISYY174 pKa = 9.76 IDD176 pKa = 3.87 EE177 pKa = 4.55 NSTLTQLDD185 pKa = 3.49 MSLAIDD191 pKa = 3.27 SWEE194 pKa = 4.0 TLTDD198 pKa = 4.08 LKK200 pKa = 11.2 LNPDD204 pKa = 4.81 NIHH207 pKa = 7.18 LDD209 pKa = 3.6 YY210 pKa = 10.88 TDD212 pKa = 3.77 EE213 pKa = 5.83 DD214 pKa = 4.67 GVTNQIDD221 pKa = 4.3 LTTLVQNLEE230 pKa = 4.11 TLTTIVANADD240 pKa = 3.3 GTFTYY245 pKa = 10.53 TDD247 pKa = 3.44 EE248 pKa = 4.83 NGNATQIDD256 pKa = 3.9 ISNLEE261 pKa = 4.23 TLTSLALNADD271 pKa = 4.08 GKK273 pKa = 8.0 TLEE276 pKa = 4.29 YY277 pKa = 9.66 TDD279 pKa = 4.0 EE280 pKa = 4.75 SGTVTTVDD288 pKa = 3.67 LSSVITNFEE297 pKa = 4.54 TITTLVDD304 pKa = 3.59 NGDD307 pKa = 3.23 GTYY310 pKa = 10.07 TYY312 pKa = 10.47 TNEE315 pKa = 4.26 SGNTTVIDD323 pKa = 3.88 VNNTEE328 pKa = 4.29 TLTEE332 pKa = 4.26 LVLSTDD338 pKa = 3.41 NSTLTYY344 pKa = 10.04 TDD346 pKa = 3.61 EE347 pKa = 4.64 NGNDD351 pKa = 3.63 TIINLKK357 pKa = 9.07 TVVQANQKK365 pKa = 6.03 TTTLADD371 pKa = 3.76 GVNTTVSSTVSGDD384 pKa = 3.1 NTAWQVNVATAKK396 pKa = 10.34 GANNTQNSTLGVVKK410 pKa = 10.15 EE411 pKa = 4.14 ADD413 pKa = 3.58 SNPTVNIANDD423 pKa = 3.66 GSLSVNLEE431 pKa = 3.62 NTNDD435 pKa = 3.3 IKK437 pKa = 10.96 EE438 pKa = 4.04 VSGNYY443 pKa = 8.2 TVLPNDD449 pKa = 4.2 TILLGNASTADD460 pKa = 3.53 VSIVMPNPVGKK471 pKa = 9.99 KK472 pKa = 10.26 GKK474 pKa = 8.71 TITIKK479 pKa = 10.81 KK480 pKa = 9.56 QDD482 pKa = 3.8 TNEE485 pKa = 3.75 DD486 pKa = 4.14 YY487 pKa = 11.3 YY488 pKa = 10.83 INVYY492 pKa = 10.98 GNINGLSQLYY502 pKa = 7.91 TALPYY507 pKa = 10.78 SGWQLTSDD515 pKa = 3.84 GTQWKK520 pKa = 7.93 ITNKK524 pKa = 9.86 FF525 pKa = 3.15
Molecular weight: 56.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.732
IPC_protein 3.77
Toseland 3.528
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.579
Grimsley 3.439
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.139
Thurlkill 3.592
EMBOSS 3.719
Sillero 3.884
Patrickios 0.757
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.783
Protein with the highest isoelectric point:
>tr|A0A2W1KG00|A0A2W1KG00_9FLAO Uncharacterized protein OS=Mesonia sp. K7 OX=2218606 GN=DNG35_05730 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.15 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 KK13 pKa = 8.29 NKK15 pKa = 9.34 HH16 pKa = 4.03 GFRR19 pKa = 11.84 EE20 pKa = 4.01 RR21 pKa = 11.84 MATVNGRR28 pKa = 11.84 KK29 pKa = 9.18 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 KK40 pKa = 7.97 KK41 pKa = 10.73 LSVSSEE47 pKa = 3.76 NRR49 pKa = 11.84 HH50 pKa = 4.47 KK51 pKa = 10.5 HH52 pKa = 4.49
Molecular weight: 6.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2375
0
2375
780029
38
2477
328.4
37.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.245 ± 0.049
0.72 ± 0.015
5.429 ± 0.04
7.275 ± 0.058
5.234 ± 0.046
6.076 ± 0.055
1.819 ± 0.024
8.0 ± 0.048
8.027 ± 0.09
9.16 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.126 ± 0.026
6.214 ± 0.056
3.333 ± 0.035
3.874 ± 0.033
3.295 ± 0.032
6.104 ± 0.042
5.726 ± 0.074
6.194 ± 0.036
0.961 ± 0.02
4.189 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here