Capybara microvirus Cap1_SP_106
Average proteome isoelectric point is 5.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7Q1|A0A4P8W7Q1_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_106 OX=2584804 PE=4 SV=1
MM1 pKa = 7.38 VNRR4 pKa = 11.84 LYY6 pKa = 10.74 TFYY9 pKa = 11.32 NKK11 pKa = 10.56 ASLRR15 pKa = 11.84 YY16 pKa = 9.4 GDD18 pKa = 4.07 VFCYY22 pKa = 8.61 PTDD25 pKa = 3.75 SFCVARR31 pKa = 11.84 LKK33 pKa = 10.94 EE34 pKa = 4.09 MSGKK38 pKa = 9.68 IDD40 pKa = 3.72 FNEE43 pKa = 3.78 VDD45 pKa = 3.93 VCCVGTIDD53 pKa = 4.2 IEE55 pKa = 4.36 SGIISGFPPVRR66 pKa = 11.84 VPVSIDD72 pKa = 3.18 TSIDD76 pKa = 3.76 SQCSNSS82 pKa = 3.26
Molecular weight: 9.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.337
IPC2_protein 4.635
IPC_protein 4.431
Toseland 4.228
ProMoST 4.584
Dawson 4.418
Bjellqvist 4.571
Wikipedia 4.355
Rodwell 4.266
Grimsley 4.151
Solomon 4.406
Lehninger 4.355
Nozaki 4.533
DTASelect 4.774
Thurlkill 4.291
EMBOSS 4.368
Sillero 4.546
Patrickios 0.896
IPC_peptide 4.406
IPC2_peptide 4.533
IPC2.peptide.svr19 4.47
Protein with the highest isoelectric point:
>tr|A0A4P8W4E3|A0A4P8W4E3_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_106 OX=2584804 PE=4 SV=1
MM1 pKa = 7.39 SCVKK5 pKa = 10.2 PLFRR9 pKa = 11.84 KK10 pKa = 9.87 DD11 pKa = 3.57 YY12 pKa = 10.05 KK13 pKa = 10.89 GLYY16 pKa = 9.26 VPIPCGYY23 pKa = 10.69 CMSCRR28 pKa = 11.84 LRR30 pKa = 11.84 RR31 pKa = 11.84 SQDD34 pKa = 2.8 LSFTMNVNLEE44 pKa = 3.94 TAYY47 pKa = 10.09 KK48 pKa = 9.64 KK49 pKa = 10.58 GASASFVTLTYY60 pKa = 9.96 SEE62 pKa = 4.28 RR63 pKa = 11.84 HH64 pKa = 4.8 VPRR67 pKa = 11.84 TDD69 pKa = 3.24 GNPLPRR75 pKa = 11.84 KK76 pKa = 9.51 SKK78 pKa = 10.91 APSLPPAPLVGSAPQGLEE96 pKa = 3.77 TLRR99 pKa = 11.84 KK100 pKa = 9.46 KK101 pKa = 10.53 DD102 pKa = 3.39 LQDD105 pKa = 3.09 FFKK108 pKa = 10.86 RR109 pKa = 11.84 LRR111 pKa = 11.84 KK112 pKa = 9.51 RR113 pKa = 11.84 LASDD117 pKa = 4.63 GYY119 pKa = 11.66 SNIKK123 pKa = 10.08 YY124 pKa = 8.69 LACGEE129 pKa = 4.27 YY130 pKa = 10.65 GDD132 pKa = 4.06 KK133 pKa = 10.77 EE134 pKa = 4.42 KK135 pKa = 11.61 YY136 pKa = 10.09 NGGLGRR142 pKa = 11.84 PHH144 pKa = 5.47 YY145 pKa = 10.17 HH146 pKa = 6.76 IGIIGIDD153 pKa = 3.59 KK154 pKa = 10.8 SYY156 pKa = 11.36 LDD158 pKa = 4.2 TVLSDD163 pKa = 2.83 LWIFGTYY170 pKa = 10.22 DD171 pKa = 3.35 VGVLKK176 pKa = 10.79 QGGFNYY182 pKa = 7.63 ITKK185 pKa = 9.57 YY186 pKa = 9.89 FSKK189 pKa = 10.48 GVLGITSNTVYY200 pKa = 10.78 LDD202 pKa = 3.58 RR203 pKa = 11.84 GLEE206 pKa = 3.81 RR207 pKa = 11.84 PFIVRR212 pKa = 11.84 SQHH215 pKa = 7.04 LCDD218 pKa = 3.91 DD219 pKa = 4.24 WLRR222 pKa = 11.84 SNSHH226 pKa = 5.56 QVMKK230 pKa = 10.76 NYY232 pKa = 10.16 TYY234 pKa = 10.78 INNGKK239 pKa = 8.89 EE240 pKa = 4.1 VPIPSYY246 pKa = 10.52 IRR248 pKa = 11.84 RR249 pKa = 11.84 QLDD252 pKa = 2.87 IDD254 pKa = 3.72 SHH256 pKa = 5.93 YY257 pKa = 11.28 KK258 pKa = 10.05 PDD260 pKa = 3.86 FSGLFAEE267 pKa = 4.68 AKK269 pKa = 9.73 KK270 pKa = 10.48 RR271 pKa = 11.84 HH272 pKa = 6.01 LRR274 pKa = 11.84 LADD277 pKa = 3.3 TRR279 pKa = 11.84 DD280 pKa = 3.44 YY281 pKa = 11.71 LSFIKK286 pKa = 10.6 NYY288 pKa = 10.22 EE289 pKa = 4.16 CTLKK293 pKa = 10.71 NIKK296 pKa = 10.04 SAMPVEE302 pKa = 4.91 DD303 pKa = 3.2 VTLNLKK309 pKa = 10.72 SPVLPAKK316 pKa = 10.15 YY317 pKa = 9.8 DD318 pKa = 3.45 EE319 pKa = 5.33 KK320 pKa = 10.93 IALKK324 pKa = 10.48 YY325 pKa = 9.71 YY326 pKa = 10.55 EE327 pKa = 5.13 KK328 pKa = 10.64 INLKK332 pKa = 9.88 IVSSLGYY339 pKa = 9.67 SPEE342 pKa = 3.96 VVDD345 pKa = 4.79 FLEE348 pKa = 4.21 NKK350 pKa = 9.42 KK351 pKa = 10.62 LCEE354 pKa = 4.35 LYY356 pKa = 10.93 VSIVSQPKK364 pKa = 9.59 LISWSEE370 pKa = 3.52 AAQLLDD376 pKa = 3.72 YY377 pKa = 11.07 PPFF380 pKa = 5.33
Molecular weight: 43.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.719
IPC2_protein 8.741
IPC_protein 8.609
Toseland 9.136
ProMoST 9.063
Dawson 9.487
Bjellqvist 9.311
Wikipedia 9.692
Rodwell 9.663
Grimsley 9.575
Solomon 9.516
Lehninger 9.472
Nozaki 9.355
DTASelect 9.238
Thurlkill 9.326
EMBOSS 9.604
Sillero 9.472
Patrickios 4.291
IPC_peptide 9.516
IPC2_peptide 8.009
IPC2.peptide.svr19 7.772
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1626
82
580
271.0
30.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.289 ± 0.929
1.784 ± 0.548
7.38 ± 0.446
4.428 ± 0.471
4.736 ± 0.705
6.212 ± 0.265
1.23 ± 0.183
5.597 ± 0.506
6.027 ± 1.153
8.18 ± 0.921
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.784 ± 0.316
5.535 ± 0.375
4.244 ± 0.767
4.305 ± 1.287
4.613 ± 0.514
11.562 ± 0.791
4.182 ± 0.378
6.15 ± 0.963
0.861 ± 0.244
5.904 ± 0.646
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here