Faeces associated gemycircularvirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus porci2; Porcine associated gemycircularvirus 2

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1YRY5|T1YRY5_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 2 OX=1391031 PE=3 SV=1
MM1 pKa = 7.59PPKK4 pKa = 9.78PAYY7 pKa = 9.28FVQSRR12 pKa = 11.84YY13 pKa = 10.12VLLTYY18 pKa = 9.15AQCGDD23 pKa = 3.48LDD25 pKa = 3.38AWAVNDD31 pKa = 3.99HH32 pKa = 6.7LSFLGAEE39 pKa = 4.43CIIGRR44 pKa = 11.84EE45 pKa = 4.04LHH47 pKa = 6.88ADD49 pKa = 3.84DD50 pKa = 4.86GTHH53 pKa = 6.64LHH55 pKa = 6.74AFCDD59 pKa = 5.35FSRR62 pKa = 11.84KK63 pKa = 8.65FRR65 pKa = 11.84SRR67 pKa = 11.84RR68 pKa = 11.84SDD70 pKa = 3.22VFDD73 pKa = 3.2VGGRR77 pKa = 11.84HH78 pKa = 6.08PNVMPSFGRR87 pKa = 11.84PEE89 pKa = 4.4GGWDD93 pKa = 3.39YY94 pKa = 10.83ATKK97 pKa = 10.84DD98 pKa = 3.25GDD100 pKa = 3.74VCAGGLPRR108 pKa = 11.84PSGRR112 pKa = 11.84GVYY115 pKa = 10.06EE116 pKa = 4.89DD117 pKa = 3.54VSSWSIIVSQEE128 pKa = 3.9SEE130 pKa = 4.43GEE132 pKa = 3.81FWEE135 pKa = 4.98CVARR139 pKa = 11.84LDD141 pKa = 5.14PKK143 pKa = 10.89ALCTNYY149 pKa = 10.53GSLRR153 pKa = 11.84AYY155 pKa = 10.43ANWKK159 pKa = 8.74YY160 pKa = 10.66RR161 pKa = 11.84PAPVQYY167 pKa = 9.46EE168 pKa = 4.24HH169 pKa = 7.25PAGIKK174 pKa = 10.2FEE176 pKa = 4.66LGNVPEE182 pKa = 4.19LVVWRR187 pKa = 11.84DD188 pKa = 2.78IALGVDD194 pKa = 3.7RR195 pKa = 11.84EE196 pKa = 4.11RR197 pKa = 11.84GILVIFGDD205 pKa = 3.67TRR207 pKa = 11.84LGKK210 pKa = 7.65TLWARR215 pKa = 11.84SIGPHH220 pKa = 6.89IYY222 pKa = 10.2TIGQMSGEE230 pKa = 4.15VLLRR234 pKa = 11.84DD235 pKa = 4.84GPDD238 pKa = 2.84ADD240 pKa = 3.79YY241 pKa = 11.53AVFDD245 pKa = 4.39DD246 pKa = 4.11MRR248 pKa = 11.84GGLEE252 pKa = 4.55FFHH255 pKa = 6.92GWKK258 pKa = 9.71EE259 pKa = 3.86WFGCQSVVTVKK270 pKa = 10.58KK271 pKa = 10.58LYY273 pKa = 9.92RR274 pKa = 11.84DD275 pKa = 3.77PVQMPWGKK283 pKa = 9.35PVVWLSNRR291 pKa = 11.84DD292 pKa = 3.4PRR294 pKa = 11.84EE295 pKa = 3.72EE296 pKa = 3.94LRR298 pKa = 11.84DD299 pKa = 4.12GITNHH304 pKa = 5.76TSMGKK309 pKa = 8.81QAAIEE314 pKa = 4.34GDD316 pKa = 3.96IKK318 pKa = 10.76WLDD321 pKa = 3.56GNCIFVEE328 pKa = 4.06LDD330 pKa = 3.15HH331 pKa = 7.64AIFRR335 pKa = 11.84ANTEE339 pKa = 3.84

Molecular weight: 38.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1YRY5|T1YRY5_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 2 OX=1391031 PE=3 SV=1
MM1 pKa = 7.77AYY3 pKa = 10.07ARR5 pKa = 11.84SRR7 pKa = 11.84AKK9 pKa = 10.54RR10 pKa = 11.84GTGRR14 pKa = 11.84SKK16 pKa = 10.79KK17 pKa = 10.14RR18 pKa = 11.84GGLRR22 pKa = 11.84PRR24 pKa = 11.84YY25 pKa = 8.65RR26 pKa = 11.84KK27 pKa = 9.56KK28 pKa = 10.31RR29 pKa = 11.84PAYY32 pKa = 8.77RR33 pKa = 11.84KK34 pKa = 9.66KK35 pKa = 10.77GAMTKK40 pKa = 10.33KK41 pKa = 10.33RR42 pKa = 11.84ILNLTSTKK50 pKa = 10.2KK51 pKa = 10.4RR52 pKa = 11.84NTMLQVANTSSVNGGPVTPGPGPYY76 pKa = 9.64VVSDD80 pKa = 3.84VNGSQYY86 pKa = 11.48SVFMPTAQDD95 pKa = 3.94LNDD98 pKa = 3.5NNGAVNTISNQAQRR112 pKa = 11.84TATTCFIKK120 pKa = 10.78GFNEE124 pKa = 4.54RR125 pKa = 11.84IRR127 pKa = 11.84IQTSSGIPWFWRR139 pKa = 11.84RR140 pKa = 11.84ICFRR144 pKa = 11.84ARR146 pKa = 11.84NTTFFLYY153 pKa = 9.59STTDD157 pKa = 3.43TPSSTTGPAYY167 pKa = 9.85TEE169 pKa = 4.38TSNGMQRR176 pKa = 11.84LYY178 pKa = 10.96MNLTINQSGQTIGNILGVLFRR199 pKa = 11.84GQTGIDD205 pKa = 2.99WVDD208 pKa = 3.38PQTASVDD215 pKa = 3.57TTRR218 pKa = 11.84VDD220 pKa = 3.57LVFDD224 pKa = 3.25KK225 pKa = 10.83RR226 pKa = 11.84YY227 pKa = 8.31TIKK230 pKa = 10.64SGNASGTVRR239 pKa = 11.84DD240 pKa = 3.78FNIWHH245 pKa = 7.34RR246 pKa = 11.84YY247 pKa = 7.27GKK249 pKa = 10.06NLVYY253 pKa = 10.79DD254 pKa = 4.24DD255 pKa = 5.35DD256 pKa = 5.22EE257 pKa = 5.66NGSTEE262 pKa = 3.66ATRR265 pKa = 11.84YY266 pKa = 10.47NSVNDD271 pKa = 3.42KK272 pKa = 11.02RR273 pKa = 11.84GGGDD277 pKa = 3.89LLIFDD282 pKa = 4.91IFTPGTGAGTADD294 pKa = 4.3LLQLTSTSTLYY305 pKa = 9.69WHH307 pKa = 7.1EE308 pKa = 4.2KK309 pKa = 9.32

Molecular weight: 34.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

648

309

339

324.0

36.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.173 ± 0.431

1.543 ± 0.575

6.79 ± 0.827

3.858 ± 1.437

4.321 ± 0.281

10.031 ± 0.207

1.852 ± 0.773

4.784 ± 0.045

4.63 ± 0.559

6.636 ± 0.52

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.852 ± 0.058

4.784 ± 1.499

4.784 ± 0.578

3.086 ± 0.511

8.025 ± 0.458

6.327 ± 0.716

7.562 ± 3.038

6.481 ± 0.836

2.623 ± 0.645

3.858 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski