Undibacterium pigrum
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5704 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A318JBN9|A0A318JBN9_9BURK Amino acid/amide ABC transporter substrate-binding protein (HAAT family) OS=Undibacterium pigrum OX=401470 GN=DFR42_102218 PE=4 SV=1
TT1 pKa = 7.14 YY2 pKa = 7.63 NTTEE6 pKa = 3.92 NGVNLNAQSTANAVARR22 pKa = 11.84 VVTDD26 pKa = 4.23 TINLGAGNDD35 pKa = 3.74 TLVTYY40 pKa = 9.55 GAINLAGAQLSNIEE54 pKa = 4.63 NITSNSAVVITASQYY69 pKa = 10.69 AALIAARR76 pKa = 11.84 AALNLSGPVLTFSGVGPHH94 pKa = 6.06 QLTIVDD100 pKa = 4.56 DD101 pKa = 3.98 VAGANNIDD109 pKa = 3.98 LSFISITGGTLVYY122 pKa = 10.69 DD123 pKa = 3.89 VTSSSNATGGGVNNTTTSGAVTVSGSGVAIAGTIGTSPSNGGGQAANGTALTAGGTFNGTAGLNEE188 pKa = 4.38 NFTGNAAALVGTTVNGNNGDD208 pKa = 3.7 TDD210 pKa = 4.43 TITISGGGTVNIGSGNTITNIKK232 pKa = 8.21 TLTMDD237 pKa = 3.62 SAAANVVFFSVANSGITTVNGGSGNDD263 pKa = 3.66 YY264 pKa = 11.26 VDD266 pKa = 4.34 LANSGNSMLAGNVNLGTGTNTLVMEE291 pKa = 5.0 GKK293 pKa = 8.63 TYY295 pKa = 10.45 TGTFVSGSGTSDD307 pKa = 2.84 TLYY310 pKa = 10.92 LFNGSNISAANVSGFEE326 pKa = 4.14 TVNINNNASVTMTAAQFAGFTTFTAGGTEE355 pKa = 4.37 TVNLTTAGTVTANANVEE372 pKa = 4.16 NYY374 pKa = 10.01 VLANGSNTFTGAAGLISVNGGTGSDD399 pKa = 4.01 TINVSSVMISATVAPNGINGGLGADD424 pKa = 3.94 TLNVSAVTAALDD436 pKa = 3.48 MSAKK440 pKa = 8.0 VTGVEE445 pKa = 4.38 TVNVTGGTNAAWTVTNEE462 pKa = 3.64 NGAGVTLNFTKK473 pKa = 10.67 SAANDD478 pKa = 3.42 INNITLGSGGQTLNILGTGTGRR500 pKa = 11.84 TTITGGSGADD510 pKa = 3.77 IINLSATATGADD522 pKa = 4.14 AIALGSNINAIDD534 pKa = 3.54 TVNNFKK540 pKa = 10.83 AAGADD545 pKa = 3.71 LFATGVVPTSLNNLSIANADD565 pKa = 3.76 SSNLAAAIATAATAAGATLANTGQAYY591 pKa = 8.61 TIVVNSGTAAGIYY604 pKa = 10.02 AFQNTGGSVGAVDD617 pKa = 3.37 TGDD620 pKa = 4.34 FIVKK624 pKa = 10.53 LGGTTGTIFATDD636 pKa = 3.68 FGIANAVAVTPGGTFNGTAGANDD659 pKa = 3.47 IFMSTIPGLNGTTIAGNAADD679 pKa = 4.34 TDD681 pKa = 4.16 VLTLTTAGTVTINNGSTGGTLSNLKK706 pKa = 9.93 VLNLANGTNTITYY719 pKa = 7.22 NTSAGFTTINGGSGDD734 pKa = 3.91 DD735 pKa = 3.47 TFIPNTALLPIVVSGGLGTDD755 pKa = 4.11 TIVLSAAYY763 pKa = 9.47 AAVASGSGTFAGNVTGFEE781 pKa = 4.29 KK782 pKa = 10.84 LRR784 pKa = 11.84 LTSVTNQTIDD794 pKa = 3.29 LQTLGNYY801 pKa = 10.24 SDD803 pKa = 3.85 VTFSGANGLTMSNLPSNGNITLNGTGTAFTISNAAFAGGVNDD845 pKa = 4.4 IVNLTLTDD853 pKa = 3.69 ASTSAVAFATTGITASGVEE872 pKa = 4.37 TFNITTVDD880 pKa = 3.62 GQATPTGLFNDD891 pKa = 3.99 TLTILGNSVKK901 pKa = 10.56 NIYY904 pKa = 10.29 VSGNAGLTMPATSTSLINVDD924 pKa = 3.48 ASGITLGGFTWTANALTSTATVKK947 pKa = 10.78 GSASGTNTVNMNSATAGVDD966 pKa = 3.54 YY967 pKa = 10.98 TGGTGNDD974 pKa = 3.57 NITINATVSSTAALGGGNNSLALNGVTLLGTYY1006 pKa = 7.55 TAGNTGTDD1014 pKa = 3.04 SLAFFSSTPDD1024 pKa = 2.96 ISNATITGFEE1034 pKa = 4.22 NLTVVNNANITMTAAQMAQFTGTVNASGTEE1064 pKa = 4.29 TINLTTAGTVTAFSAVEE1081 pKa = 4.26 KK1082 pKa = 11.03 YY1083 pKa = 10.95 NLANGTNNFTSANVAVSVIGGTGTDD1108 pKa = 3.41 TFNFTANQIANFLTTVDD1125 pKa = 4.43 GGNGTDD1131 pKa = 3.59 TLNVGATTTQNLDD1144 pKa = 3.24 FSTKK1148 pKa = 9.12 IASIEE1153 pKa = 4.08 IINVAGSTGTASVTNPDD1170 pKa = 3.54 GAGVTLNYY1178 pKa = 8.62 TKK1180 pKa = 10.01 STGDD1184 pKa = 3.23 NTITLGTGGQTLNLLGSSASSTTVTGGAAVDD1215 pKa = 4.26 TINLQFSGSGSEE1227 pKa = 4.13 TLIEE1231 pKa = 4.18 TGANMSNRR1239 pKa = 11.84 TQVDD1243 pKa = 2.88 IVGNFNSTGTDD1254 pKa = 3.49 YY1255 pKa = 11.36 FKK1257 pKa = 10.72 TGVNAVSVGSYY1268 pKa = 10.24 IIGNADD1274 pKa = 3.2 TGNYY1278 pKa = 7.23 LTTIASGLSIVLNNTGQSYY1297 pKa = 10.7 LITIQTGTAAGTYY1310 pKa = 9.74 LFQNTGSDD1318 pKa = 3.12 TSQFDD1323 pKa = 3.3 NTDD1326 pKa = 3.63 FFVQLTGTIGGIGAGNLIAA1345 pKa = 5.87
Molecular weight: 131.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.516
IPC_protein 3.567
Toseland 3.325
ProMoST 3.757
Dawson 3.579
Bjellqvist 3.732
Wikipedia 3.554
Rodwell 3.389
Grimsley 3.236
Solomon 3.579
Lehninger 3.528
Nozaki 3.694
DTASelect 3.999
Thurlkill 3.389
EMBOSS 3.554
Sillero 3.694
Patrickios 0.846
IPC_peptide 3.567
IPC2_peptide 3.668
IPC2.peptide.svr19 3.685
Protein with the highest isoelectric point:
>tr|A0A318JMY3|A0A318JMY3_9BURK Uncharacterized protein OS=Undibacterium pigrum OX=401470 GN=DFR42_102579 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.57 RR14 pKa = 11.84 THH16 pKa = 5.79 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.77 GRR39 pKa = 11.84 KK40 pKa = 8.8 RR41 pKa = 11.84 LAAA44 pKa = 4.42
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5704
0
5704
1883445
27
4271
330.2
36.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.924 ± 0.044
0.938 ± 0.011
5.266 ± 0.026
5.41 ± 0.034
3.883 ± 0.023
7.163 ± 0.035
2.23 ± 0.018
5.638 ± 0.024
4.798 ± 0.031
10.609 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.701 ± 0.015
3.748 ± 0.024
4.37 ± 0.023
4.534 ± 0.03
5.253 ± 0.024
6.339 ± 0.026
5.343 ± 0.031
6.735 ± 0.031
1.343 ± 0.014
2.773 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here