Tea plant line pattern virus
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A386QVH1|A0A386QVH1_9BROM Coat protein OS=Tea plant line pattern virus OX=2419940 PE=3 SV=1
MM1 pKa = 7.84 DD2 pKa = 3.74 VKK4 pKa = 10.89 SWLSSLSSSSLDD16 pKa = 3.55 PLRR19 pKa = 11.84 EE20 pKa = 3.98 ALLCDD25 pKa = 4.36 DD26 pKa = 5.58 DD27 pKa = 4.32 MPIDD31 pKa = 3.59 KK32 pKa = 10.98 VEE34 pKa = 4.2 EE35 pKa = 3.98 NTHH38 pKa = 5.43 ISRR41 pKa = 11.84 FIGWVILRR49 pKa = 11.84 KK50 pKa = 10.07 VFFSEE55 pKa = 4.42 EE56 pKa = 3.98 FASDD60 pKa = 3.21 EE61 pKa = 4.51 YY62 pKa = 11.73 YY63 pKa = 10.57 FYY65 pKa = 11.35 SDD67 pKa = 3.7 CLRR70 pKa = 11.84 LRR72 pKa = 11.84 FFEE75 pKa = 4.42 LFSVPMEE82 pKa = 4.7 LYY84 pKa = 10.26 DD85 pKa = 4.89 ANDD88 pKa = 3.55 SGSTNVVFVEE98 pKa = 4.67 SDD100 pKa = 2.67 ASYY103 pKa = 9.84 YY104 pKa = 10.88 KK105 pKa = 10.75 EE106 pKa = 4.59 MEE108 pKa = 4.55 DD109 pKa = 4.33 LDD111 pKa = 4.36 LAFSFPSEE119 pKa = 4.3 DD120 pKa = 3.68 KK121 pKa = 11.12 RR122 pKa = 11.84 GDD124 pKa = 3.72 VNDD127 pKa = 4.11 SSPDD131 pKa = 3.56 SVEE134 pKa = 3.88 LTEE137 pKa = 4.2 VSQVEE142 pKa = 4.19 VEE144 pKa = 4.42 PYY146 pKa = 10.89 LDD148 pKa = 4.66 FKK150 pKa = 11.76 LEE152 pKa = 4.08 VCTLPDD158 pKa = 4.34 DD159 pKa = 4.95 EE160 pKa = 4.84 GLCRR164 pKa = 11.84 QLDD167 pKa = 3.99 WKK169 pKa = 10.69 AQTHH173 pKa = 4.28 VVVEE177 pKa = 4.14 NVLRR181 pKa = 11.84 NSSIEE186 pKa = 4.15 IINSMRR192 pKa = 11.84 TYY194 pKa = 10.66 NANCIQDD201 pKa = 4.76 AIDD204 pKa = 3.56 EE205 pKa = 4.53 VFPHH209 pKa = 6.66 HH210 pKa = 6.87 FSVDD214 pKa = 2.95 DD215 pKa = 4.38 RR216 pKa = 11.84 YY217 pKa = 10.54 FQTFVEE223 pKa = 4.61 TSDD226 pKa = 3.79 IDD228 pKa = 4.18 LEE230 pKa = 4.63 LDD232 pKa = 3.35 AAHH235 pKa = 7.24 IDD237 pKa = 3.43 MSKK240 pKa = 10.33 FPKK243 pKa = 10.47 KK244 pKa = 10.3 FDD246 pKa = 3.07 IHH248 pKa = 6.96 FDD250 pKa = 3.64 WEE252 pKa = 4.59 PKK254 pKa = 10.48 LSTGLTSTRR263 pKa = 11.84 YY264 pKa = 9.71 NSFRR268 pKa = 11.84 EE269 pKa = 3.64 AALAIKK275 pKa = 9.8 KK276 pKa = 10.33 RR277 pKa = 11.84 NLNVPDD283 pKa = 4.06 LTADD287 pKa = 3.97 LDD289 pKa = 3.98 VDD291 pKa = 4.1 SLVPKK296 pKa = 10.4 VMEE299 pKa = 4.54 RR300 pKa = 11.84 FLKK303 pKa = 10.58 ILDD306 pKa = 3.84 LGKK309 pKa = 10.88 LSGFPDD315 pKa = 4.21 DD316 pKa = 4.38 SHH318 pKa = 8.75 DD319 pKa = 3.84 VGCFLLDD326 pKa = 3.39 YY327 pKa = 10.56 LSRR330 pKa = 11.84 KK331 pKa = 8.55 GLSYY335 pKa = 11.24 DD336 pKa = 3.54 VLEE339 pKa = 4.78 PGCMVSLNKK348 pKa = 9.26 YY349 pKa = 7.76 RR350 pKa = 11.84 HH351 pKa = 5.72 MIKK354 pKa = 10.08 SQLKK358 pKa = 8.85 PVEE361 pKa = 4.15 EE362 pKa = 5.05 DD363 pKa = 3.21 SLHH366 pKa = 6.21 VEE368 pKa = 4.27 RR369 pKa = 11.84 PLAATITYY377 pKa = 9.36 HH378 pKa = 6.34 GKK380 pKa = 10.1 EE381 pKa = 4.29 KK382 pKa = 11.08 VSVTTPFFLSLAAKK396 pKa = 8.71 LLCVLSDD403 pKa = 3.42 KK404 pKa = 11.13 VVIPMGKK411 pKa = 8.33 EE412 pKa = 3.48 HH413 pKa = 6.79 QLFDD417 pKa = 3.84 INVDD421 pKa = 3.37 VFRR424 pKa = 11.84 KK425 pKa = 9.19 IKK427 pKa = 8.68 FWKK430 pKa = 10.02 EE431 pKa = 2.77 IDD433 pKa = 3.36 YY434 pKa = 11.51 SKK436 pKa = 10.92 FDD438 pKa = 3.38 KK439 pKa = 11.22 SQGKK443 pKa = 8.33 LHH445 pKa = 6.86 HH446 pKa = 6.86 EE447 pKa = 3.78 IQRR450 pKa = 11.84 AIFRR454 pKa = 11.84 DD455 pKa = 3.91 LQCDD459 pKa = 3.44 EE460 pKa = 5.22 GFLSTWFHH468 pKa = 4.99 SHH470 pKa = 5.81 EE471 pKa = 4.39 FSTIFDD477 pKa = 3.87 SEE479 pKa = 4.27 AGIGFSTDD487 pKa = 3.49 FQRR490 pKa = 11.84 RR491 pKa = 11.84 TGDD494 pKa = 2.74 ACTYY498 pKa = 10.46 LGNTLVTLATLSFVYY513 pKa = 10.57 DD514 pKa = 4.17 LDD516 pKa = 4.55 SPDD519 pKa = 3.06 VGLIVVSGDD528 pKa = 3.37 DD529 pKa = 3.51 SLIGTFHH536 pKa = 7.07 SEE538 pKa = 3.92 LPRR541 pKa = 11.84 EE542 pKa = 3.98 RR543 pKa = 11.84 EE544 pKa = 4.09 ILCSTLFNFEE554 pKa = 3.96 TKK556 pKa = 10.16 FPHH559 pKa = 5.6 NQPFICSKK567 pKa = 10.23 FLVEE571 pKa = 5.95 IEE573 pKa = 4.34 TTDD576 pKa = 3.24 GRR578 pKa = 11.84 GKK580 pKa = 10.33 VVAVPSPIKK589 pKa = 10.64 LLMKK593 pKa = 10.51 LGPKK597 pKa = 10.07 SMNKK601 pKa = 9.25 QLFPAWVEE609 pKa = 4.25 SIKK612 pKa = 10.95 DD613 pKa = 3.34 YY614 pKa = 11.13 AAPFRR619 pKa = 11.84 NAFVAEE625 pKa = 4.22 QVCNLASYY633 pKa = 10.79 RR634 pKa = 11.84 FGRR637 pKa = 11.84 RR638 pKa = 11.84 CFTFLLPAISEE649 pKa = 4.44 VLWMLSNPNVKK660 pKa = 10.27 LKK662 pKa = 10.92 EE663 pKa = 3.94 KK664 pKa = 10.82 LFLQNSSYY672 pKa = 11.09 LSNHH676 pKa = 4.96 CHH678 pKa = 7.04 DD679 pKa = 3.93 RR680 pKa = 11.84 RR681 pKa = 11.84 EE682 pKa = 4.2 KK683 pKa = 9.75 EE684 pKa = 4.03 KK685 pKa = 10.87 KK686 pKa = 9.41 RR687 pKa = 11.84 NRR689 pKa = 11.84 PRR691 pKa = 11.84 ARR693 pKa = 11.84 SKK695 pKa = 10.78 RR696 pKa = 3.69
Molecular weight: 80.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.087
IPC2_protein 5.092
IPC_protein 5.08
Toseland 5.054
ProMoST 5.245
Dawson 5.105
Bjellqvist 5.219
Wikipedia 5.003
Rodwell 5.016
Grimsley 4.991
Solomon 5.105
Lehninger 5.067
Nozaki 5.232
DTASelect 5.423
Thurlkill 5.08
EMBOSS 5.067
Sillero 5.308
Patrickios 4.088
IPC_peptide 5.118
IPC2_peptide 5.308
IPC2.peptide.svr19 5.245
Protein with the highest isoelectric point:
>tr|A0A386QVU9|A0A386QVU9_9BROM Movement protein OS=Tea plant line pattern virus OX=2419940 PE=4 SV=1
MM1 pKa = 7.13 STNTQQKK8 pKa = 8.25 KK9 pKa = 6.54 TRR11 pKa = 11.84 QNKK14 pKa = 9.44 RR15 pKa = 11.84 STQFGQNRR23 pKa = 11.84 AKK25 pKa = 10.32 QRR27 pKa = 11.84 AQSTGEE33 pKa = 3.96 SSTVPNFGTLRR44 pKa = 11.84 LEE46 pKa = 4.16 WVRR49 pKa = 11.84 RR50 pKa = 11.84 GPGDD54 pKa = 4.42 LDD56 pKa = 3.15 ILQFPYY62 pKa = 10.43 QWVAKK67 pKa = 10.37 AEE69 pKa = 4.21 SSLKK73 pKa = 9.72 ATSNDD78 pKa = 1.88 IWYY81 pKa = 9.62 IIDD84 pKa = 3.16 LHH86 pKa = 6.75 EE87 pKa = 4.18 YY88 pKa = 8.7 VKK90 pKa = 11.41 DD91 pKa = 3.39 MMEE94 pKa = 4.4 FPTSVKK100 pKa = 10.24 GFVFLFEE107 pKa = 5.36 ANLSGQACLVTKK119 pKa = 9.54 KK120 pKa = 9.21 TGSFKK125 pKa = 11.05 DD126 pKa = 3.44 AFSSLNAFKK135 pKa = 10.72 FEE137 pKa = 4.55 KK138 pKa = 10.27 GCHH141 pKa = 4.82 TAVQLLAPSILTFATFSAGEE161 pKa = 3.9 VALVFKK167 pKa = 11.04 FEE169 pKa = 4.18 GTLTAGAEE177 pKa = 4.02 FMTRR181 pKa = 11.84 KK182 pKa = 9.56 VWGQSSMLPKK192 pKa = 10.47 VEE194 pKa = 3.99 INKK197 pKa = 9.53 NLLRR201 pKa = 11.84 GQQ203 pKa = 3.52
Molecular weight: 22.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.898
IPC2_protein 8.946
IPC_protein 8.858
Toseland 9.926
ProMoST 9.458
Dawson 10.058
Bjellqvist 9.663
Wikipedia 10.16
Rodwell 10.701
Grimsley 10.087
Solomon 10.087
Lehninger 10.072
Nozaki 9.94
DTASelect 9.648
Thurlkill 9.94
EMBOSS 10.306
Sillero 9.984
Patrickios 10.438
IPC_peptide 10.101
IPC2_peptide 8.126
IPC2.peptide.svr19 7.883
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2230
203
1042
557.5
62.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.65 ± 0.533
2.197 ± 0.255
6.413 ± 0.937
6.233 ± 0.419
5.381 ± 0.931
4.529 ± 0.496
2.556 ± 0.418
5.336 ± 0.605
6.637 ± 0.329
9.507 ± 0.506
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.511 ± 0.276
4.619 ± 0.549
3.812 ± 0.219
3.004 ± 0.647
5.291 ± 0.2
9.552 ± 0.437
5.919 ± 0.665
7.489 ± 0.493
0.942 ± 0.231
2.422 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here