Gelidibacter gilvus
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3732 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q0XA07|A0A4Q0XA07_9FLAO IS1182 family transposase OS=Gelidibacter gilvus OX=59602 GN=ESZ48_19115 PE=4 SV=1
AA1 pKa = 8.02 QVITQDD7 pKa = 3.63 ISIDD11 pKa = 3.36 LDD13 pKa = 3.95 ANGDD17 pKa = 3.54 ASIIAAQVDD26 pKa = 3.79 NGSNDD31 pKa = 3.12 ACGIASMTVVPNTFSCSNIGANTVTLTVVDD61 pKa = 3.97 NNGNSSSATATVTVNDD77 pKa = 3.41 VTVAQVITQDD87 pKa = 3.24 ISIDD91 pKa = 3.36 LDD93 pKa = 3.95 ANGDD97 pKa = 3.54 ASIIAAQVDD106 pKa = 3.79 NGSNDD111 pKa = 3.12 ACGIASMTVVPSTFDD126 pKa = 3.22 CSNIGTNTVTLTVVDD141 pKa = 3.97 NNGNSSSATATVTVNDD157 pKa = 3.3 VTAAQVITQDD167 pKa = 3.52 ISIDD171 pKa = 3.36 LDD173 pKa = 3.95 ANGDD177 pKa = 3.54 ASIIAAQVDD186 pKa = 3.79 NGSNDD191 pKa = 3.12 ACGIASMTVVPNTFDD206 pKa = 3.67 CSNIGANTVTLTVVDD221 pKa = 4.02 VNGNSSSATATVTVNDD237 pKa = 3.36 VTLAQVITQDD247 pKa = 3.68 ISIDD251 pKa = 3.36 LDD253 pKa = 3.95 ANGDD257 pKa = 3.54 ASIIAAQVDD266 pKa = 3.79 NGSNDD271 pKa = 3.12 ACGIASMTVVPNTFDD286 pKa = 3.67 CSNIGANTVTLTVVDD301 pKa = 3.97 NNGNSSSATATVTVNDD317 pKa = 3.42 VTVAQVVTQDD327 pKa = 3.2 ISIDD331 pKa = 3.54 LDD333 pKa = 3.6 ASGDD337 pKa = 3.58 ASIIAAQVDD346 pKa = 4.1 DD347 pKa = 4.94 GSNDD351 pKa = 3.14 ACGIASMTVVPNTFDD366 pKa = 3.67 CSNIGANTVTLTVVDD381 pKa = 4.02 VNGNSSSATATVTVNDD397 pKa = 3.3 VTAAQVITQDD407 pKa = 3.52 ISIDD411 pKa = 3.36 LDD413 pKa = 3.95 ANGDD417 pKa = 3.54 ASIIAAQVDD426 pKa = 3.79 NGSNDD431 pKa = 3.12 ACGIASMTVVPNTFDD446 pKa = 3.67 CSNIGANTVTLTVVDD461 pKa = 3.97 NNGNSSSATATVTVNDD477 pKa = 3.3 VTAAQVITQNTSIDD491 pKa = 3.4 LDD493 pKa = 4.08 ANGNASIVPADD504 pKa = 3.8 VDD506 pKa = 3.65 NGSNDD511 pKa = 3.17 ACGIASMTVTPNTFDD526 pKa = 3.9 CSNIGANTVTLTVVDD541 pKa = 4.76 LNGNSNSAMATVTVNDD557 pKa = 3.93 VIAAQVITQNISLDD571 pKa = 3.42 LDD573 pKa = 4.15 ANGGASIVPADD584 pKa = 3.9 VDD586 pKa = 3.79 NGSSDD591 pKa = 3.37 ACGIASMTVTPNTFDD606 pKa = 3.7 CSNVGANTVTLTVTDD621 pKa = 3.47 VNGNVSSATANVTVNDD637 pKa = 3.63 ITPAIVITQNTSIDD651 pKa = 3.4 LDD653 pKa = 4.08 ANGNASIVPADD664 pKa = 3.8 VDD666 pKa = 3.65 NGSNDD671 pKa = 3.04 ACGIANMTVSPNTFDD686 pKa = 4.69 CSNLGMNTVTLTVTDD701 pKa = 3.66 VNGNISSATAVVSISDD717 pKa = 3.48 NTAPDD722 pKa = 3.93 IVCVPDD728 pKa = 3.5 GTRR731 pKa = 11.84 DD732 pKa = 3.56 TDD734 pKa = 3.91 PGQCTYY740 pKa = 10.04 TIQGAEE746 pKa = 3.89 FDD748 pKa = 5.84 AIFTDD753 pKa = 3.86 NCSSGVITNSINGTATLAGEE773 pKa = 4.58 VFMQGATEE781 pKa = 4.21 VTWIVDD787 pKa = 3.59 DD788 pKa = 4.21 GHH790 pKa = 6.55 GQTASCTTTITVEE803 pKa = 4.14 DD804 pKa = 4.04 NEE806 pKa = 4.43 APIVDD811 pKa = 5.13 CINIQVLLDD820 pKa = 3.46 ASGNGTITVADD831 pKa = 3.82 INNNSTDD838 pKa = 3.13 NCGIASITLSQTSFDD853 pKa = 4.26 CSDD856 pKa = 2.98 IGGDD860 pKa = 3.74 LDD862 pKa = 3.71 EE863 pKa = 6.1 LIISEE868 pKa = 4.29 YY869 pKa = 11.04 SDD871 pKa = 3.13 GTGNNDD877 pKa = 4.48 CIEE880 pKa = 4.37 IYY882 pKa = 10.71 NGTGNPINLYY892 pKa = 10.8 AEE894 pKa = 5.54 GYY896 pKa = 6.78 TLRR899 pKa = 11.84 FYY901 pKa = 11.72 YY902 pKa = 10.49 NGSTSYY908 pKa = 9.54 TQVPLLGTVADD919 pKa = 3.98 RR920 pKa = 11.84 EE921 pKa = 4.72 VYY923 pKa = 9.54 VVCNPFGPGAMQADD937 pKa = 3.7 QTGNFGFDD945 pKa = 3.27 GNDD948 pKa = 3.47 AIALTKK954 pKa = 10.45 AGNAIDD960 pKa = 4.31 VIGQIGVNPGTGWTVGSNSTAGTTLVRR987 pKa = 11.84 NKK989 pKa = 10.56 DD990 pKa = 3.51 VLYY993 pKa = 11.3 GNINDD998 pKa = 3.49 ISGISSEE1005 pKa = 3.94 WTQYY1009 pKa = 9.75 AQNNTSNLGSHH1020 pKa = 6.87 EE1021 pKa = 4.1 IEE1023 pKa = 4.38 IADD1026 pKa = 3.86 LAKK1029 pKa = 10.79 NVILTVTDD1037 pKa = 3.2 TSGNVSTCEE1046 pKa = 3.96 GNVTVIDD1053 pKa = 4.45 NIAPVAMCQSVTIQLDD1069 pKa = 3.72 ANGVASVTASEE1080 pKa = 4.45 VDD1082 pKa = 3.22 NGSNDD1087 pKa = 3.16 ACGIASLVLDD1097 pKa = 3.75 QTDD1100 pKa = 4.32 FTCANLGDD1108 pKa = 3.86 NTVILTVTDD1117 pKa = 3.61 NNGNSSSCEE1126 pKa = 3.62 ATITVEE1132 pKa = 4.75 DD1133 pKa = 4.69 NIDD1136 pKa = 3.82 PTVMTQDD1143 pKa = 3.64 LTIQLDD1149 pKa = 4.07 ANGSASITPADD1160 pKa = 4.14 IDD1162 pKa = 3.69 NGSIDD1167 pKa = 3.49 NCGIATQTVTPNSFDD1182 pKa = 3.59 CSNVGVNTVTLTVTDD1197 pKa = 3.44 TSGNVSTGIATVTVEE1212 pKa = 5.14 DD1213 pKa = 3.93 NVAPIAICKK1222 pKa = 10.16 NITVEE1227 pKa = 4.28 LGINGEE1233 pKa = 4.13 ATITGADD1240 pKa = 3.56 VDD1242 pKa = 4.41 NGSSDD1247 pKa = 3.35 ACGIASLDD1255 pKa = 3.4 VSPSVFGCADD1265 pKa = 3.02 IGANTVTLTVTDD1277 pKa = 3.61 NNGNVSTCSATVTVTGIVPVVTISQGPLPEE1307 pKa = 4.45 FCQGAVLVLTAEE1319 pKa = 4.09 SDD1321 pKa = 3.44 EE1322 pKa = 4.41 DD1323 pKa = 5.2 FGYY1326 pKa = 11.06 LWTTGEE1332 pKa = 4.34 TTQSIEE1338 pKa = 3.71 IPGNGTYY1345 pKa = 10.78 GVTVTSLTNCSTEE1358 pKa = 3.97 AEE1360 pKa = 4.08 ITITGFNAGVLVSSYY1375 pKa = 10.74 TIIASEE1381 pKa = 4.28 KK1382 pKa = 10.43 VEE1384 pKa = 4.19 LKK1386 pKa = 11.13 NNVIVQTGGVGVTKK1400 pKa = 10.68 ASGEE1404 pKa = 4.08 IKK1406 pKa = 9.92 IEE1408 pKa = 4.05 KK1409 pKa = 9.54 ASHH1412 pKa = 5.5 IVGLGQANKK1421 pKa = 10.13 IEE1423 pKa = 4.28 IKK1425 pKa = 10.28 QGSSVGTAVYY1435 pKa = 10.33 QPANPTIPAFVYY1447 pKa = 10.26 NGYY1450 pKa = 10.08 SSSSSPDD1457 pKa = 2.81 VRR1459 pKa = 11.84 VNNNQTLTLSGGVYY1473 pKa = 10.46 NKK1475 pKa = 10.06 VEE1477 pKa = 4.23 LEE1479 pKa = 4.08 EE1480 pKa = 4.19 NATVLFTASNVYY1492 pKa = 10.3 INEE1495 pKa = 4.17 LKK1497 pKa = 9.52 TKK1499 pKa = 10.22 KK1500 pKa = 10.33 SATIEE1505 pKa = 4.12 FNGCTNVFLNKK1516 pKa = 9.67 EE1517 pKa = 4.22 FKK1519 pKa = 10.89 LDD1521 pKa = 3.79 DD1522 pKa = 3.97 NGVFNSNGYY1531 pKa = 8.33 MVTVYY1536 pKa = 10.59 INDD1539 pKa = 3.52 DD1540 pKa = 3.87 FEE1542 pKa = 4.69 VKK1544 pKa = 10.25 KK1545 pKa = 11.2 GSDD1548 pKa = 3.23 FTGRR1552 pKa = 11.84 VHH1554 pKa = 6.79 TNNHH1558 pKa = 6.24 DD1559 pKa = 3.7 IEE1561 pKa = 4.83 VEE1563 pKa = 4.11 GHH1565 pKa = 5.97 NSSTTYY1571 pKa = 7.51 MTGLFIGKK1579 pKa = 9.26 KK1580 pKa = 9.73 VEE1582 pKa = 3.81 ADD1584 pKa = 3.84 KK1585 pKa = 11.54 NVVWNADD1592 pKa = 3.95 QYY1594 pKa = 11.69 CDD1596 pKa = 3.93 PCPIEE1601 pKa = 5.2 APINNGGSEE1610 pKa = 4.8 PITDD1614 pKa = 3.53 YY1615 pKa = 11.25 TIIAFDD1621 pKa = 3.88 EE1622 pKa = 4.46 VHH1624 pKa = 6.27 LHH1626 pKa = 6.69 GDD1628 pKa = 3.73 NIVQTGGVGVTRR1640 pKa = 11.84 HH1641 pKa = 5.19 KK1642 pKa = 11.0 KK1643 pKa = 9.89 KK1644 pKa = 10.53 IKK1646 pKa = 9.35 LHH1648 pKa = 6.22 KK1649 pKa = 9.85 DD1650 pKa = 2.95 SHH1652 pKa = 5.22 ITEE1655 pKa = 4.38 FAKK1658 pKa = 10.7 ASQIQVNGGSSIGTRR1673 pKa = 11.84 ILSPADD1679 pKa = 3.82 PIIPLFVKK1687 pKa = 10.38 NVYY1690 pKa = 10.51 SNSSSPNATINSGQTVTLTGSVYY1713 pKa = 11.08 DD1714 pKa = 4.39 KK1715 pKa = 11.58 VDD1717 pKa = 3.72 LKK1719 pKa = 11.24 EE1720 pKa = 3.97 GATVIFAQSNVFINEE1735 pKa = 3.91 LKK1737 pKa = 7.7 TRR1739 pKa = 11.84 KK1740 pKa = 9.39 YY1741 pKa = 10.22 ATIKK1745 pKa = 10.71 FSGCTNVFIKK1755 pKa = 10.77 KK1756 pKa = 8.76 EE1757 pKa = 4.14 FKK1759 pKa = 10.61 LDD1761 pKa = 3.62 DD1762 pKa = 3.85 YY1763 pKa = 11.89 GVINPEE1769 pKa = 3.86 TDD1771 pKa = 2.59 GHH1773 pKa = 5.84 KK1774 pKa = 10.7 VVFHH1778 pKa = 6.73 VDD1780 pKa = 3.25 DD1781 pKa = 4.75 DD1782 pKa = 4.58 VEE1784 pKa = 4.12 IDD1786 pKa = 3.31 KK1787 pKa = 11.39 GSVVVASIYY1796 pKa = 10.69 AYY1798 pKa = 10.64 NDD1800 pKa = 3.41 EE1801 pKa = 4.41 IKK1803 pKa = 10.76 VDD1805 pKa = 4.09 GSSSDD1810 pKa = 3.68 PVSMKK1815 pKa = 10.87 GLFIAKK1821 pKa = 9.21 KK1822 pKa = 8.11 VHH1824 pKa = 5.15 AHH1826 pKa = 7.15 EE1827 pKa = 4.85 DD1828 pKa = 3.75 VLWNKK1833 pKa = 8.19 DD1834 pKa = 3.45 TSGSPCPVITPTAQAPVAPEE1854 pKa = 3.6 VGNRR1858 pKa = 11.84 SIEE1861 pKa = 4.39 LISVKK1866 pKa = 10.18 AWPNPSDD1873 pKa = 3.44 TVFNLRR1879 pKa = 11.84 LITEE1883 pKa = 4.13 NQVDD1887 pKa = 4.25 VVSILVFDD1895 pKa = 4.49 SNNKK1899 pKa = 9.0 LVHH1902 pKa = 6.35 SSTFRR1907 pKa = 11.84 PEE1909 pKa = 3.46 AVYY1912 pKa = 10.61 QFGNEE1917 pKa = 4.11 LEE1919 pKa = 4.24 SGVYY1923 pKa = 8.69 IVKK1926 pKa = 10.19 VSQAGSNAYY1935 pKa = 8.71 TRR1937 pKa = 11.84 VIKK1940 pKa = 10.41 FF1941 pKa = 3.24
Molecular weight: 200.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.643
IPC_protein 3.706
Toseland 3.465
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.528
Grimsley 3.376
Solomon 3.719
Lehninger 3.668
Nozaki 3.821
DTASelect 4.126
Thurlkill 3.528
EMBOSS 3.681
Sillero 3.834
Patrickios 0.68
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A4Q0XBG4|A0A4Q0XBG4_9FLAO Queuine tRNA-ribosyltransferase OS=Gelidibacter gilvus OX=59602 GN=tgt PE=3 SV=1
MM1 pKa = 7.05 TRR3 pKa = 11.84 GIRR6 pKa = 11.84 FSVGSLQFAVFSWQSSVGSLQLAVFSWQSSVGSLQLAVFRR46 pKa = 11.84 RR47 pKa = 11.84 LL48 pKa = 3.16
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 10.701
IPC_protein 12.31
Toseland 12.471
ProMoST 12.983
Dawson 12.471
Bjellqvist 12.471
Wikipedia 12.954
Rodwell 11.974
Grimsley 12.515
Solomon 12.983
Lehninger 12.881
Nozaki 12.471
DTASelect 12.471
Thurlkill 12.471
EMBOSS 12.983
Sillero 12.471
Patrickios 11.798
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.2
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3732
0
3732
1309719
24
3758
350.9
39.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.446 ± 0.037
0.695 ± 0.013
5.821 ± 0.029
6.442 ± 0.035
5.204 ± 0.028
6.328 ± 0.037
1.951 ± 0.019
7.91 ± 0.037
7.529 ± 0.043
9.329 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.345 ± 0.021
5.948 ± 0.042
3.447 ± 0.022
3.447 ± 0.019
3.523 ± 0.024
6.552 ± 0.034
5.794 ± 0.044
6.181 ± 0.03
1.052 ± 0.013
4.057 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here