Salmon aquaparamyxovirus
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G2KTE9|A0A3G2KTE9_9MONO Hemagglutinin-neuraminidase protein OS=Salmon aquaparamyxovirus OX=381543 GN=HN PE=3 SV=1
MM1 pKa = 7.4 SSYY4 pKa = 10.73 GRR6 pKa = 11.84 SAGKK10 pKa = 10.04 DD11 pKa = 3.15 KK12 pKa = 10.89 QVEE15 pKa = 4.08 RR16 pKa = 11.84 QEE18 pKa = 4.41 TSRR21 pKa = 11.84 HH22 pKa = 4.81 PNEE25 pKa = 4.52 RR26 pKa = 11.84 EE27 pKa = 3.87 SLLDD31 pKa = 3.56 SGLSNIQSLLRR42 pKa = 11.84 DD43 pKa = 3.75 INSSTRR49 pKa = 11.84 TSQKK53 pKa = 10.68 KK54 pKa = 9.84 KK55 pKa = 10.25 LNSGADD61 pKa = 3.92 DD62 pKa = 3.63 TSSTHH67 pKa = 6.06 SASSSDD73 pKa = 3.53 RR74 pKa = 11.84 PRR76 pKa = 11.84 DD77 pKa = 3.72 NAGGTSDD84 pKa = 3.65 RR85 pKa = 11.84 RR86 pKa = 11.84 VRR88 pKa = 11.84 SQSQDD93 pKa = 2.47 AVTQRR98 pKa = 11.84 DD99 pKa = 3.86 ISSLHH104 pKa = 6.58 CDD106 pKa = 3.63 DD107 pKa = 5.06 PRR109 pKa = 11.84 SQVNCSANKK118 pKa = 10.16 RR119 pKa = 11.84 STGPPAPQPRR129 pKa = 11.84 GRR131 pKa = 11.84 DD132 pKa = 3.29 LHH134 pKa = 7.54 KK135 pKa = 10.94 GVVGVNEE142 pKa = 3.87 IPPYY146 pKa = 10.47 EE147 pKa = 5.12 GGDD150 pKa = 3.12 VDD152 pKa = 3.84 QLVNEE157 pKa = 4.54 EE158 pKa = 4.31 EE159 pKa = 4.25 EE160 pKa = 4.45 ADD162 pKa = 3.39 IPMAEE167 pKa = 4.21 QQDD170 pKa = 4.29 GADD173 pKa = 3.6 EE174 pKa = 4.11 EE175 pKa = 5.38 SYY177 pKa = 10.92 PEE179 pKa = 3.75 QAGRR183 pKa = 11.84 GSSARR188 pKa = 11.84 SSLGGGSALAGWGRR202 pKa = 11.84 QANTSSPAEE211 pKa = 3.8 ISEE214 pKa = 4.71 GYY216 pKa = 10.8 DD217 pKa = 4.33 DD218 pKa = 3.89 VEE220 pKa = 4.08 RR221 pKa = 11.84 WAKK224 pKa = 10.3 HH225 pKa = 4.13 ATLPEE230 pKa = 4.38 LFTRR234 pKa = 11.84 VQSGPNGVCSPVAIVAEE251 pKa = 4.45 EE252 pKa = 3.96 AQLLYY257 pKa = 10.14 TVEE260 pKa = 4.51 PEE262 pKa = 3.84 PVMSEE267 pKa = 4.02 EE268 pKa = 4.15 EE269 pKa = 3.77 EE270 pKa = 4.41 AMIDD274 pKa = 3.63 QALNDD279 pKa = 4.22 TLDD282 pKa = 3.36 VDD284 pKa = 4.75 SDD286 pKa = 3.97 GGDD289 pKa = 3.49 DD290 pKa = 4.25 SPLIEE295 pKa = 4.3 QMSVLLDD302 pKa = 5.88 DD303 pKa = 4.07 EE304 pKa = 5.17 QEE306 pKa = 4.22 WNNLDD311 pKa = 4.12 QNRR314 pKa = 11.84 GDD316 pKa = 3.34 QDD318 pKa = 3.87 QNQEE322 pKa = 4.11 WKK324 pKa = 10.72 DD325 pKa = 3.42 SALNIGKK332 pKa = 9.62 KK333 pKa = 6.65 GHH335 pKa = 5.85 RR336 pKa = 11.84 RR337 pKa = 11.84 EE338 pKa = 3.8 VSIYY342 pKa = 8.27 TRR344 pKa = 11.84 NGICYY349 pKa = 9.97 LDD351 pKa = 3.68 TWCNPCCVPIRR362 pKa = 11.84 VHH364 pKa = 5.37 PTRR367 pKa = 11.84 EE368 pKa = 3.5 RR369 pKa = 11.84 CICRR373 pKa = 11.84 LCPEE377 pKa = 4.31 TCGDD381 pKa = 3.86 CAVATEE387 pKa = 4.95 DD388 pKa = 3.73 PTIPSII394 pKa = 4.6
Molecular weight: 43.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.543
IPC2_protein 4.495
IPC_protein 4.457
Toseland 4.291
ProMoST 4.571
Dawson 4.418
Bjellqvist 4.571
Wikipedia 4.291
Rodwell 4.291
Grimsley 4.202
Solomon 4.418
Lehninger 4.368
Nozaki 4.52
DTASelect 4.711
Thurlkill 4.304
EMBOSS 4.317
Sillero 4.571
Patrickios 3.846
IPC_peptide 4.418
IPC2_peptide 4.571
IPC2.peptide.svr19 4.492
Protein with the highest isoelectric point:
>tr|A0A3G2KTD2|A0A3G2KTD2_9MONO V protein OS=Salmon aquaparamyxovirus OX=381543 GN=V PE=4 SV=1
MM1 pKa = 7.72 AGLLARR7 pKa = 11.84 INKK10 pKa = 8.09 WRR12 pKa = 11.84 GKK14 pKa = 9.55 KK15 pKa = 9.52 QADD18 pKa = 3.44 TPMRR22 pKa = 11.84 EE23 pKa = 4.31 SLYY26 pKa = 9.78 STQDD30 pKa = 3.02 FPTFSPSSEE39 pKa = 3.7 ISTPPPEE46 pKa = 4.27 PPKK49 pKa = 10.5 RR50 pKa = 11.84 RR51 pKa = 11.84 NLTVVPMTPRR61 pKa = 11.84 APTPPQVLTGPGTMLVVHH79 pKa = 7.09 PIGGYY84 pKa = 8.88 DD85 pKa = 3.61 PKK87 pKa = 11.27 AKK89 pKa = 9.35 TLSPSGILAAYY100 pKa = 8.99 IATIQGARR108 pKa = 11.84 SIAVLTRR115 pKa = 11.84 DD116 pKa = 4.06 LQDD119 pKa = 3.68 LLLRR123 pKa = 11.84 SPEE126 pKa = 3.63 EE127 pKa = 4.21 GIFIRR132 pKa = 11.84 EE133 pKa = 4.09 LLEE136 pKa = 3.8 STRR139 pKa = 11.84 YY140 pKa = 10.28 LLMRR144 pKa = 11.84 VEE146 pKa = 4.32 MWISLLTRR154 pKa = 11.84 KK155 pKa = 9.35 KK156 pKa = 10.43 RR157 pKa = 11.84 PTYY160 pKa = 9.16 QWLNNRR166 pKa = 11.84 MEE168 pKa = 4.04 QMKK171 pKa = 10.25 RR172 pKa = 11.84 VTLNKK177 pKa = 9.82 PVEE180 pKa = 4.38 DD181 pKa = 4.53 LLLDD185 pKa = 3.75 QAWVVVVPWLDD196 pKa = 3.41 GEE198 pKa = 4.72 GRR200 pKa = 11.84 QILPARR206 pKa = 11.84 QKK208 pKa = 10.57 SLRR211 pKa = 11.84 DD212 pKa = 3.3 MTTLRR217 pKa = 11.84 GGQSMQHH224 pKa = 5.43 SQSFSPASRR233 pKa = 11.84 ADD235 pKa = 3.42 RR236 pKa = 11.84 TEE238 pKa = 3.84 YY239 pKa = 10.28 VAQQ242 pKa = 3.89
Molecular weight: 27.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.235
IPC2_protein 9.458
IPC_protein 9.823
Toseland 10.306
ProMoST 9.97
Dawson 10.452
Bjellqvist 10.131
Wikipedia 10.628
Rodwell 10.76
Grimsley 10.526
Solomon 10.511
Lehninger 10.482
Nozaki 10.262
DTASelect 10.131
Thurlkill 10.321
EMBOSS 10.701
Sillero 10.365
Patrickios 10.438
IPC_peptide 10.511
IPC2_peptide 8.712
IPC2.peptide.svr19 8.696
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
5450
242
2213
681.3
75.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.294 ± 0.385
1.945 ± 0.273
6.11 ± 0.43
5.89 ± 0.574
2.917 ± 0.655
7.009 ± 0.615
1.725 ± 0.231
6.018 ± 0.586
5.138 ± 0.487
9.156 ± 0.463
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.789 ± 0.427
4.422 ± 0.336
4.899 ± 0.854
3.927 ± 0.369
5.266 ± 0.506
8.661 ± 0.786
6.312 ± 0.29
7.064 ± 0.362
1.229 ± 0.169
3.229 ± 0.343
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here