marine gamma proteobacterium HTCC2148

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; unclassified Halieaceae

Average proteome isoelectric point is 5.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3825 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7S188|B7S188_9GAMM Uncharacterized protein OS=marine gamma proteobacterium HTCC2148 OX=247634 GN=GPB2148_1302 PE=4 SV=1
MM1 pKa = 7.68AFFSTANIANAVVGQNDD18 pKa = 2.93IDD20 pKa = 3.7EE21 pKa = 4.8ARR23 pKa = 11.84FITLPFDD30 pKa = 3.57EE31 pKa = 5.54VFDD34 pKa = 4.09NTGLQPDD41 pKa = 4.6AEE43 pKa = 4.06NDD45 pKa = 3.46ALGEE49 pKa = 4.18SFPEE53 pKa = 4.82DD54 pKa = 3.0ICEE57 pKa = 4.11EE58 pKa = 4.31GGVGYY63 pKa = 7.7MHH65 pKa = 6.87YY66 pKa = 10.09KK67 pKa = 10.0YY68 pKa = 10.63KK69 pKa = 9.4PTEE72 pKa = 3.83DD73 pKa = 3.26QMINIIASGDD83 pKa = 3.45DD84 pKa = 3.35TEE86 pKa = 4.59IRR88 pKa = 11.84VINADD93 pKa = 3.84VPPADD98 pKa = 5.39DD99 pKa = 4.18NSCLYY104 pKa = 10.94SQDD107 pKa = 4.67DD108 pKa = 3.74DD109 pKa = 3.76NLNYY113 pKa = 10.35FEE115 pKa = 6.19GDD117 pKa = 3.37DD118 pKa = 3.86VNGVFVPDD126 pKa = 3.56TDD128 pKa = 3.35EE129 pKa = 4.28SGNYY133 pKa = 9.11IPASDD138 pKa = 4.48DD139 pKa = 3.0NSARR143 pKa = 11.84VGEE146 pKa = 4.25APHH149 pKa = 7.4WYY151 pKa = 9.53QEE153 pKa = 4.9LYY155 pKa = 10.72FSDD158 pKa = 4.43PGACTHH164 pKa = 6.81ACSEE168 pKa = 4.15DD169 pKa = 3.58TKK171 pKa = 11.46DD172 pKa = 4.59GVEE175 pKa = 4.05LTANTTYY182 pKa = 10.5IIQIAANYY190 pKa = 9.27SGRR193 pKa = 11.84GNLGDD198 pKa = 3.79EE199 pKa = 4.94CDD201 pKa = 3.81NTTLPDD207 pKa = 4.54GSCEE211 pKa = 3.9GDD213 pKa = 3.04NRR215 pKa = 11.84SPTFVSIAVGGLGAAKK231 pKa = 10.08DD232 pKa = 4.22RR233 pKa = 11.84PTLAPVTPVPTLPLFGLGILVSLLGFFGLRR263 pKa = 11.84KK264 pKa = 9.99LKK266 pKa = 10.32QQ267 pKa = 3.06

Molecular weight: 28.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7S2A5|B7S2A5_9GAMM HemY N-terminal domain protein OS=marine gamma proteobacterium HTCC2148 OX=247634 GN=GPB2148_291 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.87RR14 pKa = 11.84THH16 pKa = 5.99GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.44GGRR28 pKa = 11.84AVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3825

0

3825

1273162

29

6746

332.9

36.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.036 ± 0.042

1.075 ± 0.014

5.961 ± 0.037

6.413 ± 0.04

3.817 ± 0.026

8.043 ± 0.099

2.128 ± 0.022

5.368 ± 0.028

3.614 ± 0.027

10.299 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.637 ± 0.023

3.507 ± 0.025

4.506 ± 0.031

4.064 ± 0.026

5.631 ± 0.034

6.451 ± 0.037

5.113 ± 0.029

7.043 ± 0.032

1.394 ± 0.017

2.888 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski