Amino acid dipepetide frequency for marine gamma proteobacterium HTCC2148

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.116AlaAla: 11.116 ± 0.121	1.187AlaCys: 1.187 ± 0.032	5.876AlaAsp: 5.876 ± 0.078	6.473AlaGlu: 6.473 ± 0.09	3.41AlaPhe: 3.41 ± 0.055	8.595AlaGly: 8.595 ± 0.118	1.809AlaHis: 1.809 ± 0.043	5.537AlaIle: 5.537 ± 0.065	3.428AlaLys: 3.428 ± 0.069	10.681AlaLeu: 10.681 ± 0.107	3.038AlaMet: 3.038 ± 0.055	3.295AlaAsn: 3.295 ± 0.052	3.952AlaPro: 3.952 ± 0.063	4.062AlaGln: 4.062 ± 0.064	5.466AlaArg: 5.466 ± 0.074	6.28AlaSer: 6.28 ± 0.082	5.098AlaThr: 5.098 ± 0.074	7.222AlaVal: 7.222 ± 0.092	1.288AlaTrp: 1.288 ± 0.033	2.55AlaTyr: 2.55 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	1.071CysAla: 1.071 ± 0.03	0.142CysCys: 0.142 ± 0.012	0.725CysAsp: 0.725 ± 0.023	0.651CysGlu: 0.651 ± 0.024	0.409CysPhe: 0.409 ± 0.016	1.0CysGly: 1.0 ± 0.035	0.313CysHis: 0.313 ± 0.015	0.527CysIle: 0.527 ± 0.021	0.309CysLys: 0.309 ± 0.016	1.068CysLeu: 1.068 ± 0.033	0.239CysMet: 0.239 ± 0.014	0.357CysAsn: 0.357 ± 0.017	0.556CysPro: 0.556 ± 0.023	0.411CysGln: 0.411 ± 0.02	0.613CysArg: 0.613 ± 0.024	0.742CysSer: 0.742 ± 0.025	0.514CysThr: 0.514 ± 0.022	0.648CysVal: 0.648 ± 0.026	0.144CysTrp: 0.144 ± 0.01	0.309CysTyr: 0.309 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	5.528AspAla: 5.528 ± 0.075	0.689AspCys: 0.689 ± 0.028	3.326AspAsp: 3.326 ± 0.062	3.991AspGlu: 3.991 ± 0.062	2.544AspPhe: 2.544 ± 0.051	4.633AspGly: 4.633 ± 0.101	1.262AspHis: 1.262 ± 0.03	3.643AspIle: 3.643 ± 0.059	2.253AspLys: 2.253 ± 0.045	5.78AspLeu: 5.78 ± 0.08	1.616AspMet: 1.616 ± 0.034	2.122AspAsn: 2.122 ± 0.045	2.943AspPro: 2.943 ± 0.056	2.25AspGln: 2.25 ± 0.044	3.128AspArg: 3.128 ± 0.05	3.763AspSer: 3.763 ± 0.063	2.934AspThr: 2.934 ± 0.054	3.974AspVal: 3.974 ± 0.066	1.012AspTrp: 1.012 ± 0.029	2.219AspTyr: 2.219 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	6.192GluAla: 6.192 ± 0.081	0.492GluCys: 0.492 ± 0.02	3.499GluAsp: 3.499 ± 0.064	4.336GluGlu: 4.336 ± 0.081	2.37GluPhe: 2.37 ± 0.043	4.383GluGly: 4.383 ± 0.061	1.545GluHis: 1.545 ± 0.036	3.553GluIle: 3.553 ± 0.06	2.712GluLys: 2.712 ± 0.051	7.177GluLeu: 7.177 ± 0.073	1.599GluMet: 1.599 ± 0.037	2.236GluAsn: 2.236 ± 0.043	2.521GluPro: 2.521 ± 0.044	3.549GluGln: 3.549 ± 0.059	4.023GluArg: 4.023 ± 0.064	3.701GluSer: 3.701 ± 0.056	3.135GluThr: 3.135 ± 0.054	4.617GluVal: 4.617 ± 0.064	0.767GluTrp: 0.767 ± 0.024	1.726GluTyr: 1.726 ± 0.039	0.001GluXaa: 0.001 ± 0.001
Phe	3.599PheAla: 3.599 ± 0.066	0.489PheCys: 0.489 ± 0.022	2.743PheAsp: 2.743 ± 0.047	2.374PheGlu: 2.374 ± 0.043	1.58PhePhe: 1.58 ± 0.049	3.328PheGly: 3.328 ± 0.057	0.805PheHis: 0.805 ± 0.023	2.053PheIle: 2.053 ± 0.043	1.232PheLys: 1.232 ± 0.032	3.361PheLeu: 3.361 ± 0.064	0.998PheMet: 0.998 ± 0.032	1.525PheAsn: 1.525 ± 0.042	1.598PhePro: 1.598 ± 0.032	1.208PheGln: 1.208 ± 0.028	1.775PheArg: 1.775 ± 0.039	2.961PheSer: 2.961 ± 0.051	2.18PheThr: 2.18 ± 0.042	2.487PheVal: 2.487 ± 0.043	0.523PheTrp: 0.523 ± 0.025	1.353PheTyr: 1.353 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	7.327GlyAla: 7.327 ± 0.135	0.885GlyCys: 0.885 ± 0.026	4.799GlyAsp: 4.799 ± 0.108	5.211GlyGlu: 5.211 ± 0.08	3.409GlyPhe: 3.409 ± 0.049	6.881GlyGly: 6.881 ± 0.482	1.716GlyHis: 1.716 ± 0.039	4.695GlyIle: 4.695 ± 0.078	3.508GlyLys: 3.508 ± 0.053	8.065GlyLeu: 8.065 ± 0.086	2.392GlyMet: 2.392 ± 0.05	2.758GlyAsn: 2.758 ± 0.086	2.605GlyPro: 2.605 ± 0.045	3.023GlyGln: 3.023 ± 0.046	4.27GlyArg: 4.27 ± 0.062	5.179GlySer: 5.179 ± 0.109	3.968GlyThr: 3.968 ± 0.073	5.889GlyVal: 5.889 ± 0.087	1.247GlyTrp: 1.247 ± 0.033	2.605GlyTyr: 2.605 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.785HisAla: 1.785 ± 0.041	0.349HisCys: 0.349 ± 0.018	1.012HisAsp: 1.012 ± 0.03	1.074HisGlu: 1.074 ± 0.032	0.961HisPhe: 0.961 ± 0.032	1.799HisGly: 1.799 ± 0.036	0.604HisHis: 0.604 ± 0.023	1.119HisIle: 1.119 ± 0.03	0.675HisLys: 0.675 ± 0.023	2.212HisLeu: 2.212 ± 0.047	0.556HisMet: 0.556 ± 0.02	0.711HisAsn: 0.711 ± 0.021	1.259HisPro: 1.259 ± 0.031	0.884HisGln: 0.884 ± 0.028	1.411HisArg: 1.411 ± 0.032	1.363HisSer: 1.363 ± 0.036	0.991HisThr: 0.991 ± 0.027	1.288HisVal: 1.288 ± 0.028	0.406HisTrp: 0.406 ± 0.02	0.818HisTyr: 0.818 ± 0.025	0.001HisXaa: 0.001 ± 0.001
Ile	6.214IleAla: 6.214 ± 0.08	0.635IleCys: 0.635 ± 0.023	3.851IleAsp: 3.851 ± 0.059	3.798IleGlu: 3.798 ± 0.059	1.877IlePhe: 1.877 ± 0.04	4.315IleGly: 4.315 ± 0.073	1.071IleHis: 1.071 ± 0.033	2.688IleIle: 2.688 ± 0.052	1.926IleLys: 1.926 ± 0.049	4.384IleLeu: 4.384 ± 0.075	1.155IleMet: 1.155 ± 0.035	2.264IleAsn: 2.264 ± 0.044	2.581IlePro: 2.581 ± 0.054	1.607IleGln: 1.607 ± 0.039	2.73IleArg: 2.73 ± 0.042	3.748IleSer: 3.748 ± 0.06	3.107IleThr: 3.107 ± 0.053	3.641IleVal: 3.641 ± 0.061	0.601IleTrp: 0.601 ± 0.022	1.487IleTyr: 1.487 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	3.719LysAla: 3.719 ± 0.068	0.254LysCys: 0.254 ± 0.013	1.809LysAsp: 1.809 ± 0.035	2.008LysGlu: 2.008 ± 0.045	1.117LysPhe: 1.117 ± 0.033	2.586LysGly: 2.586 ± 0.051	0.814LysHis: 0.814 ± 0.023	1.999LysIle: 1.999 ± 0.04	1.689LysLys: 1.689 ± 0.071	3.849LysLeu: 3.849 ± 0.062	0.921LysMet: 0.921 ± 0.03	1.344LysAsn: 1.344 ± 0.032	1.896LysPro: 1.896 ± 0.046	1.61LysGln: 1.61 ± 0.042	2.301LysArg: 2.301 ± 0.052	2.268LysSer: 2.268 ± 0.044	1.97LysThr: 1.97 ± 0.041	2.629LysVal: 2.629 ± 0.05	0.399LysTrp: 0.399 ± 0.018	0.964LysTyr: 0.964 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	11.479LeuAla: 11.479 ± 0.105	1.13LeuCys: 1.13 ± 0.033	6.292LeuAsp: 6.292 ± 0.079	6.632LeuGlu: 6.632 ± 0.084	3.685LeuPhe: 3.685 ± 0.072	8.073LeuGly: 8.073 ± 0.084	2.079LeuHis: 2.079 ± 0.041	5.03LeuIle: 5.03 ± 0.073	3.773LeuLys: 3.773 ± 0.063	10.735LeuLeu: 10.735 ± 0.138	2.546LeuMet: 2.546 ± 0.045	3.615LeuAsn: 3.615 ± 0.055	5.054LeuPro: 5.054 ± 0.067	4.392LeuGln: 4.392 ± 0.071	5.753LeuArg: 5.753 ± 0.082	6.804LeuSer: 6.804 ± 0.073	5.131LeuThr: 5.131 ± 0.064	7.045LeuVal: 7.045 ± 0.081	1.287LeuTrp: 1.287 ± 0.034	2.457LeuTyr: 2.457 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	2.894MetAla: 2.894 ± 0.048	0.203MetCys: 0.203 ± 0.014	1.438MetAsp: 1.438 ± 0.035	1.533MetGlu: 1.533 ± 0.035	0.86MetPhe: 0.86 ± 0.03	2.103MetGly: 2.103 ± 0.04	0.556MetHis: 0.556 ± 0.023	1.347MetIle: 1.347 ± 0.038	1.04MetLys: 1.04 ± 0.028	2.758MetLeu: 2.758 ± 0.053	0.7MetMet: 0.7 ± 0.026	1.012MetAsn: 1.012 ± 0.028	1.331MetPro: 1.331 ± 0.033	1.088MetGln: 1.088 ± 0.035	1.495MetArg: 1.495 ± 0.033	1.909MetSer: 1.909 ± 0.038	1.55MetThr: 1.55 ± 0.034	1.812MetVal: 1.812 ± 0.044	0.244MetTrp: 0.244 ± 0.015	0.499MetTyr: 0.499 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.394AsnAla: 3.394 ± 0.058	0.403AsnCys: 0.403 ± 0.02	1.858AsnAsp: 1.858 ± 0.042	1.976AsnGlu: 1.976 ± 0.038	1.453AsnPhe: 1.453 ± 0.034	2.719AsnGly: 2.719 ± 0.074	0.71AsnHis: 0.71 ± 0.025	2.046AsnIle: 2.046 ± 0.04	1.19AsnLys: 1.19 ± 0.034	3.354AsnLeu: 3.354 ± 0.055	0.891AsnMet: 0.891 ± 0.029	1.313AsnAsn: 1.313 ± 0.031	2.085AsnPro: 2.085 ± 0.042	1.276AsnGln: 1.276 ± 0.033	2.048AsnArg: 2.048 ± 0.039	2.269AsnSer: 2.269 ± 0.048	1.994AsnThr: 1.994 ± 0.049	2.332AsnVal: 2.332 ± 0.047	0.585AsnTrp: 0.585 ± 0.024	1.177AsnTyr: 1.177 ± 0.032	0.001AsnXaa: 0.001 ± 0.001
Pro	4.364ProAla: 4.364 ± 0.065	0.378ProCys: 0.378 ± 0.018	3.125ProAsp: 3.125 ± 0.053	3.788ProGlu: 3.788 ± 0.063	1.683ProPhe: 1.683 ± 0.045	3.975ProGly: 3.975 ± 0.056	0.926ProHis: 0.926 ± 0.027	2.136ProIle: 2.136 ± 0.047	1.492ProLys: 1.492 ± 0.036	4.568ProLeu: 4.568 ± 0.059	1.209ProMet: 1.209 ± 0.028	1.514ProAsn: 1.514 ± 0.037	1.771ProPro: 1.771 ± 0.042	1.798ProGln: 1.798 ± 0.029	2.184ProArg: 2.184 ± 0.042	2.531ProSer: 2.531 ± 0.043	2.172ProThr: 2.172 ± 0.044	3.563ProVal: 3.563 ± 0.051	0.672ProTrp: 0.672 ± 0.023	1.214ProTyr: 1.214 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	4.281GlnAla: 4.281 ± 0.059	0.39GlnCys: 0.39 ± 0.018	1.96GlnAsp: 1.96 ± 0.045	2.33GlnGlu: 2.33 ± 0.05	1.466GlnPhe: 1.466 ± 0.035	3.018GlnGly: 3.018 ± 0.051	0.919GlnHis: 0.919 ± 0.031	1.925GlnIle: 1.925 ± 0.041	1.302GlnLys: 1.302 ± 0.032	4.772GlnLeu: 4.772 ± 0.071	1.082GlnMet: 1.082 ± 0.028	1.111GlnAsn: 1.111 ± 0.03	1.743GlnPro: 1.743 ± 0.038	2.341GlnGln: 2.341 ± 0.052	2.82GlnArg: 2.82 ± 0.057	2.513GlnSer: 2.513 ± 0.069	1.853GlnThr: 1.853 ± 0.044	2.982GlnVal: 2.982 ± 0.051	0.705GlnTrp: 0.705 ± 0.026	1.126GlnTyr: 1.126 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	4.929ArgAla: 4.929 ± 0.069	0.589ArgCys: 0.589 ± 0.023	3.383ArgAsp: 3.383 ± 0.051	4.157ArgGlu: 4.157 ± 0.066	2.458ArgPhe: 2.458 ± 0.049	3.835ArgGly: 3.835 ± 0.059	1.308ArgHis: 1.308 ± 0.034	3.095ArgIle: 3.095 ± 0.052	2.281ArgLys: 2.281 ± 0.043	5.998ArgLeu: 5.998 ± 0.074	1.525ArgMet: 1.525 ± 0.034	1.996ArgAsn: 1.996 ± 0.038	2.268ArgPro: 2.268 ± 0.043	2.455ArgGln: 2.455 ± 0.051	3.362ArgArg: 3.362 ± 0.071	3.429ArgSer: 3.429 ± 0.058	2.463ArgThr: 2.463 ± 0.052	3.925ArgVal: 3.925 ± 0.057	0.939ArgTrp: 0.939 ± 0.027	1.916ArgTyr: 1.916 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	6.397SerAla: 6.397 ± 0.082	0.653SerCys: 0.653 ± 0.025	3.775SerAsp: 3.775 ± 0.068	3.915SerGlu: 3.915 ± 0.054	2.601SerPhe: 2.601 ± 0.045	5.93SerGly: 5.93 ± 0.134	1.38SerHis: 1.38 ± 0.033	3.476SerIle: 3.476 ± 0.061	2.22SerLys: 2.22 ± 0.05	6.823SerLeu: 6.823 ± 0.079	1.744SerMet: 1.744 ± 0.038	2.183SerAsn: 2.183 ± 0.046	2.905SerPro: 2.905 ± 0.049	2.418SerGln: 2.418 ± 0.044	3.594SerArg: 3.594 ± 0.056	4.117SerSer: 4.117 ± 0.065	3.113SerThr: 3.113 ± 0.057	4.508SerVal: 4.508 ± 0.086	0.905SerTrp: 0.905 ± 0.029	1.854SerTyr: 1.854 ± 0.038	0.001SerXaa: 0.001 ± 0.001
Thr	5.177ThrAla: 5.177 ± 0.075	0.525ThrCys: 0.525 ± 0.02	3.103ThrAsp: 3.103 ± 0.057	3.1ThrGlu: 3.1 ± 0.051	1.82ThrPhe: 1.82 ± 0.036	4.723ThrGly: 4.723 ± 0.079	1.049ThrHis: 1.049 ± 0.033	2.671ThrIle: 2.671 ± 0.055	1.327ThrLys: 1.327 ± 0.033	5.673ThrLeu: 5.673 ± 0.076	1.226ThrMet: 1.226 ± 0.033	1.588ThrAsn: 1.588 ± 0.041	2.846ThrPro: 2.846 ± 0.05	1.804ThrGln: 1.804 ± 0.042	2.815ThrArg: 2.815 ± 0.046	3.088ThrSer: 3.088 ± 0.058	2.619ThrThr: 2.619 ± 0.045	3.864ThrVal: 3.864 ± 0.068	0.714ThrTrp: 0.714 ± 0.03	1.393ThrTyr: 1.393 ± 0.039	0.001ThrXaa: 0.001 ± 0.001
Val	7.327ValAla: 7.327 ± 0.078	0.801ValCys: 0.801 ± 0.028	4.539ValAsp: 4.539 ± 0.066	4.46ValGlu: 4.46 ± 0.07	2.708ValPhe: 2.708 ± 0.056	5.212ValGly: 5.212 ± 0.096	1.375ValHis: 1.375 ± 0.034	4.221ValIle: 4.221 ± 0.068	2.462ValLys: 2.462 ± 0.046	6.833ValLeu: 6.833 ± 0.083	1.83ValMet: 1.83 ± 0.045	2.641ValAsn: 2.641 ± 0.051	3.201ValPro: 3.201 ± 0.055	2.23ValGln: 2.23 ± 0.041	3.51ValArg: 3.51 ± 0.056	4.944ValSer: 4.944 ± 0.079	4.117ValThr: 4.117 ± 0.08	5.359ValVal: 5.359 ± 0.073	0.824ValTrp: 0.824 ± 0.027	1.84ValTyr: 1.84 ± 0.031	0.001ValXaa: 0.001 ± 0.001
Trp	1.063TrpAla: 1.063 ± 0.034	0.176TrpCys: 0.176 ± 0.011	0.773TrpAsp: 0.773 ± 0.025	0.745TrpGlu: 0.745 ± 0.024	0.525TrpPhe: 0.525 ± 0.022	0.99TrpGly: 0.99 ± 0.039	0.355TrpHis: 0.355 ± 0.02	0.619TrpIle: 0.619 ± 0.023	0.442TrpLys: 0.442 ± 0.019	1.762TrpLeu: 1.762 ± 0.042	0.405TrpMet: 0.405 ± 0.019	0.5TrpAsn: 0.5 ± 0.02	0.598TrpPro: 0.598 ± 0.024	0.845TrpGln: 0.845 ± 0.028	0.981TrpArg: 0.981 ± 0.03	0.949TrpSer: 0.949 ± 0.029	0.661TrpThr: 0.661 ± 0.022	0.934TrpVal: 0.934 ± 0.032	0.21TrpTrp: 0.21 ± 0.012	0.407TrpTyr: 0.407 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.507TyrAla: 2.507 ± 0.054	0.379TyrCys: 0.379 ± 0.017	1.724TyrAsp: 1.724 ± 0.043	1.582TyrGlu: 1.582 ± 0.044	1.236TyrPhe: 1.236 ± 0.032	2.334TyrGly: 2.334 ± 0.046	0.682TyrHis: 0.682 ± 0.022	1.303TyrIle: 1.303 ± 0.033	0.882TyrLys: 0.882 ± 0.03	3.139TyrLeu: 3.139 ± 0.059	0.699TyrMet: 0.699 ± 0.022	0.987TyrAsn: 0.987 ± 0.032	1.351TyrPro: 1.351 ± 0.034	1.387TyrGln: 1.387 ± 0.035	2.032TyrArg: 2.032 ± 0.043	1.951TyrSer: 1.951 ± 0.043	1.555TyrThr: 1.555 ± 0.037	1.714TyrVal: 1.714 ± 0.035	0.467TyrTrp: 0.467 ± 0.021	0.966TyrTyr: 0.966 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.096XaaXaa: 0.096 ± 0.057

Statistics based on 3825 proteins (1273162 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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