Proteome-pI 2.0 - Proteome Isoelectric Point Database

Erwinia billingiae (strain Eb661)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Erwinia; Erwinia billingiae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 4915 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|D8MWM6|D8MWM6_ERWBE DNA helicase OS=Erwinia billingiae (strain Eb661) OX=634500 GN=EbC_37020 PE=4 SV=1

MM1 pKa = 7.67NIGLFYY7 pKa = 10.91GSSTCYY13 pKa = 10.49TEE15 pKa = 3.84MAAEE19 pKa = 5.11KK20 pKa = 10.16IRR22 pKa = 11.84DD23 pKa = 4.29FIGEE27 pKa = 4.21DD28 pKa = 3.73LVTLHH33 pKa = 6.33NLKK36 pKa = 10.63DD37 pKa = 3.77DD38 pKa = 4.37APQLMEE44 pKa = 4.34QYY46 pKa = 11.77DD47 pKa = 4.12MLILGIPTWDD57 pKa = 3.34FGEE60 pKa = 4.35LQEE63 pKa = 4.51DD64 pKa = 4.18WEE66 pKa = 4.97AIWTEE71 pKa = 4.19LPALNLQGKK80 pKa = 8.37VVALYY85 pKa = 11.15GMGDD89 pKa = 3.11QGEE92 pKa = 4.2YY93 pKa = 10.79SEE95 pKa = 4.9WFLDD99 pKa = 3.85ALGMLHH105 pKa = 7.16DD106 pKa = 4.33VLMPSGVKK114 pKa = 9.38FVGYY118 pKa = 9.01WPNEE122 pKa = 3.96GYY124 pKa = 10.81EE125 pKa = 4.26FTSKK129 pKa = 10.78KK130 pKa = 10.14PLTSDD135 pKa = 3.47GSHH138 pKa = 6.06FVGLALDD145 pKa = 5.65DD146 pKa = 4.27INQFDD151 pKa = 3.85ATDD154 pKa = 3.26EE155 pKa = 5.63RR156 pKa = 11.84IAQWCEE162 pKa = 3.59QILTEE167 pKa = 4.28MVEE170 pKa = 4.39SLL172 pKa = 4.07

Molecular weight: 19.54 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|D8MLY6|D8MLY6_ERWBE Conserved uncharacterized protein OS=Erwinia billingiae (strain Eb661) OX=634500 GN=EbC_03390 PE=4 SV=1

MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGQQVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84SRR41 pKa = 11.84LTVSKK46 pKa = 11.03

Molecular weight: 5.37 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
4915	0	4915	1558479	37	6067	317.1	34.89

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
10.12 ± 0.049	0.962 ± 0.011	5.222 ± 0.03	5.423 ± 0.041	3.808 ± 0.025	7.564 ± 0.04	2.192 ± 0.019	5.601 ± 0.028	4.108 ± 0.029	10.945 ± 0.047

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.73 ± 0.018	3.647 ± 0.029	4.517 ± 0.026	4.613 ± 0.032	5.559 ± 0.041	6.29 ± 0.032	5.445 ± 0.047	7.101 ± 0.033	1.493 ± 0.015	2.66 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski