Aureimonas jatrophae
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4295 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H0K3Y5|A0A1H0K3Y5_9RHIZ Voltage-gated potassium channel OS=Aureimonas jatrophae OX=1166073 GN=SAMN05192530_10779 PE=4 SV=1
MM1 pKa = 7.32 AAAYY5 pKa = 7.96 TGTRR9 pKa = 11.84 WGASDD14 pKa = 4.13 AAGTPGGVVTWSFNLLGAEE33 pKa = 3.92 FFGFDD38 pKa = 4.08 EE39 pKa = 5.66 SILSAPFQAAVRR51 pKa = 11.84 AAFDD55 pKa = 3.33 VWEE58 pKa = 4.31 SLANIDD64 pKa = 3.89 FQEE67 pKa = 4.19 VATASAQIQLGWDD80 pKa = 3.49 TFDD83 pKa = 3.84 GAGGTLALAYY93 pKa = 8.44 WQYY96 pKa = 11.61 QGAKK100 pKa = 8.06 TLQAEE105 pKa = 4.49 VAFDD109 pKa = 4.06 IAEE112 pKa = 4.12 NWNPTAGGASFQAVAIHH129 pKa = 6.67 EE130 pKa = 4.43 IGHH133 pKa = 6.83 AIGLAHH139 pKa = 6.92 TDD141 pKa = 3.65 DD142 pKa = 4.77 PTSIMYY148 pKa = 8.75 PYY150 pKa = 10.71 LSAQTLPSASDD161 pKa = 2.95 IAAIQGLYY169 pKa = 10.24 GPSDD173 pKa = 3.42 RR174 pKa = 11.84 GFSLPGTAGNDD185 pKa = 2.84 ILTGGRR191 pKa = 11.84 GNDD194 pKa = 3.5 VISGLEE200 pKa = 4.07 GADD203 pKa = 3.49 TLQGGLGRR211 pKa = 11.84 DD212 pKa = 3.5 VLQGNQGDD220 pKa = 4.43 DD221 pKa = 3.75 LVQGGHH227 pKa = 7.09 DD228 pKa = 3.7 GDD230 pKa = 4.3 EE231 pKa = 4.32 VSGGQGNDD239 pKa = 2.63 WVYY242 pKa = 11.67 GNLGNDD248 pKa = 3.56 TLSGNLGNDD257 pKa = 3.42 VLSGGAGADD266 pKa = 3.07 TFVFMPGSGADD277 pKa = 2.9 IVTDD281 pKa = 3.55 YY282 pKa = 11.75 AFAEE286 pKa = 4.35 GDD288 pKa = 3.53 RR289 pKa = 11.84 LNVGGRR295 pKa = 11.84 TYY297 pKa = 11.42 AVATASDD304 pKa = 4.33 GSALLQLADD313 pKa = 3.62 GDD315 pKa = 4.41 IIILQGVTQGEE326 pKa = 4.58 FSQSYY331 pKa = 7.79 VAA333 pKa = 5.39
Molecular weight: 34.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.592
IPC_protein 3.63
Toseland 3.389
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.605
Rodwell 3.452
Grimsley 3.3
Solomon 3.63
Lehninger 3.592
Nozaki 3.757
DTASelect 4.037
Thurlkill 3.465
EMBOSS 3.605
Sillero 3.757
Patrickios 1.1
IPC_peptide 3.617
IPC2_peptide 3.732
IPC2.peptide.svr19 3.713
Protein with the highest isoelectric point:
>tr|A0A1H0KQ96|A0A1H0KQ96_9RHIZ Uncharacterized protein OS=Aureimonas jatrophae OX=1166073 GN=SAMN05192530_10890 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 8.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.05 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4295
0
4295
1370096
31
2376
319.0
34.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.222 ± 0.046
0.735 ± 0.011
5.798 ± 0.03
5.891 ± 0.036
3.651 ± 0.025
8.902 ± 0.034
1.901 ± 0.021
4.531 ± 0.024
2.231 ± 0.028
10.472 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.23 ± 0.015
2.231 ± 0.021
5.39 ± 0.029
2.835 ± 0.021
8.377 ± 0.042
5.303 ± 0.03
5.307 ± 0.028
7.795 ± 0.03
1.246 ± 0.015
1.952 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here