Vibrio virus VpV262
Average proteome isoelectric point is 5.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8LT38|Q8LT38_9CAUD Uncharacterized protein OS=Vibrio virus VpV262 OX=194802 PE=4 SV=1
MM1 pKa = 7.55 LVTGNGTFPLAQGANWSASALYY23 pKa = 9.99 TDD25 pKa = 4.35 ALGGATVQLQVNGVDD40 pKa = 3.98 LVDD43 pKa = 4.95 GALADD48 pKa = 3.53 NTQYY52 pKa = 11.37 VVTHH56 pKa = 6.19 GKK58 pKa = 9.07 RR59 pKa = 11.84 AVVEE63 pKa = 4.36 VVVTGYY69 pKa = 8.11 TADD72 pKa = 3.85 FNIWTYY78 pKa = 11.15 DD79 pKa = 3.15 ASS81 pKa = 3.6
Molecular weight: 8.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.792
IPC2_protein 3.973
IPC_protein 3.834
Toseland 3.63
ProMoST 4.062
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.884
Rodwell 3.681
Grimsley 3.554
Solomon 3.834
Lehninger 3.795
Nozaki 4.024
DTASelect 4.291
Thurlkill 3.732
EMBOSS 3.884
Sillero 3.973
Patrickios 0.604
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.865
Protein with the highest isoelectric point:
>tr|Q8LT57|Q8LT57_9CAUD Uncharacterized protein OS=Vibrio virus VpV262 OX=194802 PE=4 SV=1
MM1 pKa = 7.26 SKK3 pKa = 10.54 AVRR6 pKa = 11.84 FIGGAVQVIAGIYY19 pKa = 6.19 TANPALIASGVGSIAGGIAEE39 pKa = 4.75 QKK41 pKa = 10.54 RR42 pKa = 11.84 EE43 pKa = 4.05 DD44 pKa = 3.6 AAKK47 pKa = 10.37 QAVQEE52 pKa = 3.93 QRR54 pKa = 11.84 KK55 pKa = 8.77 AVAINNAQQAIQRR68 pKa = 11.84 QRR70 pKa = 11.84 QIRR73 pKa = 11.84 QTIAEE78 pKa = 4.04 ARR80 pKa = 11.84 VRR82 pKa = 11.84 RR83 pKa = 11.84 ALIQSRR89 pKa = 11.84 GFEE92 pKa = 4.34 GGPAGAGDD100 pKa = 4.72 SIYY103 pKa = 11.16 GDD105 pKa = 3.43 AASAIGAANTQQAAAFGISAARR127 pKa = 11.84 NRR129 pKa = 11.84 AAMFGLEE136 pKa = 3.97 ARR138 pKa = 11.84 SSNSFDD144 pKa = 3.52 AFAGAANLFGQGYY157 pKa = 5.65 TAYY160 pKa = 9.8 KK161 pKa = 9.87 GGSFEE166 pKa = 4.64 GLFGSGTADD175 pKa = 3.12 TGRR178 pKa = 11.84 TSDD181 pKa = 4.46 FGRR184 pKa = 11.84 SGNNSANFLEE194 pKa = 4.61 FGGAVSGGRR203 pKa = 3.44
Molecular weight: 20.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 9.531
IPC_protein 10.306
Toseland 10.365
ProMoST 10.321
Dawson 10.526
Bjellqvist 10.248
Wikipedia 10.745
Rodwell 10.687
Grimsley 10.599
Solomon 10.613
Lehninger 10.57
Nozaki 10.335
DTASelect 10.248
Thurlkill 10.394
EMBOSS 10.774
Sillero 10.438
Patrickios 10.394
IPC_peptide 10.613
IPC2_peptide 9.004
IPC2.peptide.svr19 8.817
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
67
0
67
14092
46
1536
210.3
23.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.501 ± 0.506
1.072 ± 0.178
7.245 ± 0.241
7.181 ± 0.296
3.655 ± 0.128
7.387 ± 0.384
2.25 ± 0.211
5.081 ± 0.179
5.954 ± 0.392
7.827 ± 0.254
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.824 ± 0.166
4.435 ± 0.254
3.626 ± 0.147
4.052 ± 0.282
5.23 ± 0.226
5.329 ± 0.183
6.209 ± 0.397
7.039 ± 0.27
1.476 ± 0.114
3.626 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here